Journal: Clinical and Translational Medicine
Article Title: Time‐restricted feeding improves the reproductive function of female mice via liver fibroblast growth factor 21
doi: 10.1002/ctm2.195
Figure Lengend Snippet: Time‐restricted feeding prevents hypothalamic hypogonadism. Female mice were fed with chow diet or high‐fat diet for 22 weeks with or without TRF treatment. (A) Serum LH concentrations at diestrus and proestrus; (B) GnRH test: blood LH levels at basal or 30 min after intraperitoneal GnRH injection (0.25 μg) in female mice; (C) Kisspeptin test: blood LH levels at basal and 30 min after intraperitoneal Kisspeptin (1 nmol) injection in female mice; Serum FGF21 (D), leptin (E), and adiponectin (F) levels at diestrus and proestrus; Gnrh (G) and Kiss‐1 (H) mRNA expression levels in hypothalamus during proestrus, n = 6–9 /group. (I) Culture medium GnRH levels after rFGF21 treatment for 12h in GT1‐7 cell line; (J) Culture medium GnRH level, cells were pretreated with inhibitors (U0126) 30 min before rFGF21 (50 nM) treatment and were treated with rFGF21 plus inhibitors for 12h. Data are means ± SEM; Statistical significance was evaluated by Unpaired t‐test. * P < 0.05, ** P < 0.01, *** P < 0.001
Article Snippet: GT1‐7 cells were treated with rFGF21 (8409; Bio‐Techne, Minneapolis, MN, USA) with or without the ERK 1/2 inhibitor U0126 (U120; Sigma, St. Louis, MO, USA; 10 μM).
Techniques: Injection, Expressing