jak2 stat3 inhibitor  (MedChemExpress)

Journal: Neurotherapeutics

Article Title: Reticuline modulates astrocyte and microglial responses to enhance prognosis after traumatic brain injury

doi: 10.1016/j.neurot.2025.e00709

Figure Lengend Snippet: Reticuline promoted tissue repair and inhibited inflammatory cell infiltration after TBI. (A) The number of cells in the Sham group was detected by HE staining. (B–F) After intraperitoneal injection of saline, reticuline, NT219, STAT3-IN-1, and STAT3-IN-12, respectively, and following the creation of the CCI model, the number of inflammatory cells in the lesion site as well as the area of lesion site discovered by HE staining (n ​= ​3). (G) Counts of inflammatory cells in the lesion site after different treatments. (H) Statistics of the area of lesion site after different treatments (∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001; ∗∗∗∗P ​< ​0.0001). One-way ANOVA for (G, H).

Article Snippet: Reticuline (MCE, HY-N1356) was dissolved in DMSO at a concentration of 10 mg/ml to prepare the stock solution, and the working solution was prepared using a solution of 10 % DMSO stock solution + 40 % PEG300/PEG400 + 5 % Tween-80 + 45 % saline.

Techniques: Staining, Injection, Saline

Journal: Neurotherapeutics

Article Title: Reticuline modulates astrocyte and microglial responses to enhance prognosis after traumatic brain injury

doi: 10.1016/j.neurot.2025.e00709

Figure Lengend Snippet: Reticuline promoted neurological recovery and suppressed neuroinflammation in TBI rats. (A–C) After CCI modeling and intervention with reticuline, the number of island penetrations and target quadrant dwell time of rats were detected using the Morris Water Maze test (n ​= ​12). (D–E) Statistics of de-adhesion experiments in 1-day, 7-day, and 14-day rats after TBI (n ​= ​12). (F–I) ELISA to identify the presence of IL-4, IL-10, IL-1β, and TNF-α in injured brain tissue (n ​= ​5). (∗P ​< ​0.0 5; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001; ∗∗∗∗P ​< ​0.0001). One-way ANOVA for (B–I). ELISA, enzyme-linked immunosorbent assay; IL-4, interleukin-4; TNF-α, tumor necrosis factor-α.

Article Snippet: Reticuline (MCE, HY-N1356) was dissolved in DMSO at a concentration of 10 mg/ml to prepare the stock solution, and the working solution was prepared using a solution of 10 % DMSO stock solution + 40 % PEG300/PEG400 + 5 % Tween-80 + 45 % saline.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Neurotherapeutics

Article Title: Reticuline modulates astrocyte and microglial responses to enhance prognosis after traumatic brain injury

doi: 10.1016/j.neurot.2025.e00709

Figure Lengend Snippet: Reticuline treatment after TBI can inhibit the inflammatory state of microglia. (A–B) Immunofluorescence detection of CD86 expression in the lesion site and co-localization of IBA1 (red) and CD86 (green) in the sham, control, and treatment groups (n ​= ​6). (C–D) Immunofluorescence detection of CD206 expression in the lesion site and co-localization of IBA1 (red) and CD206 (green) (n ​= ​6).(E–F) Western blot detection of CD86, iNOS, CD206, Arg1 expression in brain tissue in the lesion site (n ​= ​3). (G) Mean fluorescence intensity statistics of CD86 and CD206 (n ​= ​6). (H–I) CD86, iNOS, CD206, Arg1 grayscale value statistics. (J–K) Microglia were stimulated with LPS, followed by the administration of reticuline and detection of CD86 and CD206 expression by immunofluorescence (n ​= ​3). (∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001; ∗∗∗∗P ​< ​0.0001). One-way ANOVA for (G, H, I, K).

Article Snippet: Reticuline (MCE, HY-N1356) was dissolved in DMSO at a concentration of 10 mg/ml to prepare the stock solution, and the working solution was prepared using a solution of 10 % DMSO stock solution + 40 % PEG300/PEG400 + 5 % Tween-80 + 45 % saline.

Techniques: Immunofluorescence, Expressing, Control, Western Blot, Fluorescence

Journal: Neurotherapeutics

Article Title: Reticuline modulates astrocyte and microglial responses to enhance prognosis after traumatic brain injury

doi: 10.1016/j.neurot.2025.e00709

Figure Lengend Snippet: Reticuline treatment after TBI can inhibit the inflammatory state of astrocytes. (A–B) Immunofluorescence detection of C3 expression in the lesion site and co-localization of GFAP and C3 (n ​= ​6). (C–D) Immunofluorescence detection of S100A10 expression in the lesion site and co-localization of GFAP and S100A10 (n ​= ​6). (E–F) C3 and S100A10 expression in the lesion site of the brain tissue can be found using a Western blot. (n ​= ​3). (G) Mean fluorescence intensity statistics of C3 and S100A10. (H) C3 and S100A10 ​Gy value statistics. (I–J) Astrocytes were stimulated with LPS, followed by the administration of reticuline and detection of CD86 and CD206 expression by immunofluorescence (n ​= ​3). (∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001; ∗∗∗∗P ​< ​0.0001). One-way ANOVA for (G, H, J).

Article Snippet: Reticuline (MCE, HY-N1356) was dissolved in DMSO at a concentration of 10 mg/ml to prepare the stock solution, and the working solution was prepared using a solution of 10 % DMSO stock solution + 40 % PEG300/PEG400 + 5 % Tween-80 + 45 % saline.

Techniques: Immunofluorescence, Expressing, Western Blot, Fluorescence

Journal: Neurotherapeutics

Article Title: Reticuline modulates astrocyte and microglial responses to enhance prognosis after traumatic brain injury

doi: 10.1016/j.neurot.2025.e00709

Figure Lengend Snippet: Reticuline exerted its function by inhibiting the phosphorylation of STAT3. (A) Microglia exert their anti-inflammatory functions after TBI through STAT3 phosphorylation, which phosphorylates downstream ERK1/2 and IRF3 while inhibiting AKT phosphorylation. (B) Astrocytes exert their anti-inflammatory functions after TBI through STAT3 phosphorylation, which phosphorylates downstream ERK1/2 and p65 while inhibiting AKT phosphorylation. (C) Molecular docking results of reticuline with target molecules.

Article Snippet: Reticuline (MCE, HY-N1356) was dissolved in DMSO at a concentration of 10 mg/ml to prepare the stock solution, and the working solution was prepared using a solution of 10 % DMSO stock solution + 40 % PEG300/PEG400 + 5 % Tween-80 + 45 % saline.

Techniques: Phospho-proteomics

Journal: Neurotherapeutics

Article Title: Reticuline modulates astrocyte and microglial responses to enhance prognosis after traumatic brain injury

doi: 10.1016/j.neurot.2025.e00709

Figure Lengend Snippet: Reticuline promoted AKT phosphorylation in microglia while inhibiting STAT3, ERK1/2, and IRF3 phosphorylation. (A–D) Primary microglia were stimulated with LPS and then administered reticuline. Immunofluorescence was used to identify the expression of p-STAT3, p -ERK1/2, p -AKT, and p -IRF3; the fluorescence intensity was then quantified (n ​= ​3). (E–H) The protein expression of p-STAT3, p -ERK1/2, p -AKT, and p -IRF3 was discovered using a Western blot. Grayscale values were then tallied (n ​= ​3). (∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001; ∗∗∗∗P ​< ​0.0001). One-way ANOVA for (A–H).

Article Snippet: Reticuline (MCE, HY-N1356) was dissolved in DMSO at a concentration of 10 mg/ml to prepare the stock solution, and the working solution was prepared using a solution of 10 % DMSO stock solution + 40 % PEG300/PEG400 + 5 % Tween-80 + 45 % saline.

Techniques: Phospho-proteomics, Immunofluorescence, Expressing, Fluorescence, Western Blot

Journal: Neurotherapeutics

Article Title: Reticuline modulates astrocyte and microglial responses to enhance prognosis after traumatic brain injury

doi: 10.1016/j.neurot.2025.e00709

Figure Lengend Snippet: Reticuline inhibited the phosphorylation of STAT3, ERK1/2, and p65 while promoting AKT phosphorylation in astrocytes. (A–D) LPS was used to excite primary astrocytes, and reticuline was given to them. Immunofluorescence was used to identify the expression of p-STAT3, p -ERK1/2, p -AKT, and p -IRF3; the fluorescence intensity was then quantified (n ​= ​3). (E–H) Grayscale values were tallied after a Western blot was used to identify the expression of p-STAT3, p -ERK1/2, p -AKT, and p -IRF3 (n ​= ​3). (∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001; ∗∗∗∗P ​< ​0.0001). One-way ANOVA for (A–H).

Article Snippet: Reticuline (MCE, HY-N1356) was dissolved in DMSO at a concentration of 10 mg/ml to prepare the stock solution, and the working solution was prepared using a solution of 10 % DMSO stock solution + 40 % PEG300/PEG400 + 5 % Tween-80 + 45 % saline.

Techniques: Phospho-proteomics, Immunofluorescence, Expressing, Fluorescence, Western Blot

Journal: Neurotherapeutics

Article Title: Reticuline modulates astrocyte and microglial responses to enhance prognosis after traumatic brain injury

doi: 10.1016/j.neurot.2025.e00709

Figure Lengend Snippet: The ability of reticuline to reduce neuroinflammation was attenuated after re-stimulation of STAT3 phosphorylation. (A–B) After intraperitoneal injection of reticuline and reticuline ​+ ​Colivelin, HE staining was used to determine the number of cells and the size of the lesion site (n ​= ​3). (C) Data on the number of cells in the lesion site following various treatments. (D) Statistics of the area of lesion site after different treatments. (E–I) Primary microglia were stimulated with LPS followed by administration of reticuline or reticuline ​+ ​Colivelin. Immunofluorescence detected the expression of p-STAT3, p -ERK1/2, p -AKT, and p -IRF3, and the fluorescence intensity was measured (n ​= ​3). (J–N) Primary astrocytes were stimulated with LPS followed by administration of reticuline or reticuline ​+ ​Colivelin. Immunofluorescence detected the expression of p-STAT3, p -ERK1/2, p -AKT, and p-65, and the fluorescence intensity was measured (n ​= ​3). (∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001; ∗∗∗∗P ​< ​0.0001). Unpaired t -test for (C–D, F–I, K–N).

Article Snippet: Reticuline (MCE, HY-N1356) was dissolved in DMSO at a concentration of 10 mg/ml to prepare the stock solution, and the working solution was prepared using a solution of 10 % DMSO stock solution + 40 % PEG300/PEG400 + 5 % Tween-80 + 45 % saline.

Techniques: Phospho-proteomics, Injection, Staining, Immunofluorescence, Expressing, Fluorescence

Journal: Neurotherapeutics

Article Title: Reticuline modulates astrocyte and microglial responses to enhance prognosis after traumatic brain injury

doi: 10.1016/j.neurot.2025.e00709

Figure Lengend Snippet: The therapeutic effect of reticuline was inhibited after re-stimulation of STAT3 phosphorylation. (A–F) Swimming speed, escape latency, number of island penetrations, and target quadrant dwell time in rats detected by the Morris water maze test (n ​= ​12). (G–H) Statistics of de-adhesion experiments in 1-day, 7-day, and 14-day rats after TBI (n ​= ​12). (∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001; ∗∗∗∗P ​< ​0.0001). Unpaired t -test for (C–F), Two-way ANOVA for (G–H).

Article Snippet: Reticuline (MCE, HY-N1356) was dissolved in DMSO at a concentration of 10 mg/ml to prepare the stock solution, and the working solution was prepared using a solution of 10 % DMSO stock solution + 40 % PEG300/PEG400 + 5 % Tween-80 + 45 % saline.

Techniques: Phospho-proteomics

Journal: Neurotherapeutics

Article Title: Reticuline modulates astrocyte and microglial responses to enhance prognosis after traumatic brain injury

doi: 10.1016/j.neurot.2025.e00709

Figure Lengend Snippet: A schematic model showing the mechanism of reticuline-mediated reduction of neuroinflammation after traumatic brain injury. Reticuline plays a vital role in neuroprotection and immunomodulation. After TBI onset, reticuline modulates the inflammatory state of microglia and astrocytes by inhibiting STAT3, ERK1/2, P65, and IRF3 phosphorylation and promoting AKT phosphorylation in them. This significantly promoted the suppression of neuroinflammation and the recovery of neurological function in TBI rats.

Article Snippet: Reticuline (MCE, HY-N1356) was dissolved in DMSO at a concentration of 10 mg/ml to prepare the stock solution, and the working solution was prepared using a solution of 10 % DMSO stock solution + 40 % PEG300/PEG400 + 5 % Tween-80 + 45 % saline.

Techniques: Phospho-proteomics

Journal: RSC Advances

Article Title: Characterization and quantification of the phytochemical constituents and anti-inflammatory properties of Lindera aggregata †

doi: 10.1039/d4ra05643d

Figure Lengend Snippet: Identification of compounds in LAE using UHPLC-HRESI-Q-orbitrap-MS in positive mode

Article Snippet: Boldine (P/N, X23N9Y73113), isolinderalactone (P/N, S30HB196732), reticuline (P/N, N28HB202502), linderone (P/N, D0HB03066), and methyllinderone (P/N, D0HB03065) were purchase from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China).

Techniques:

Journal: RSC Advances

Article Title: Characterization and quantification of the phytochemical constituents and anti-inflammatory properties of Lindera aggregata †

doi: 10.1039/d4ra05643d

Figure Lengend Snippet: MS/MS spectra and the proposed fragmentation pathway of norisoboldine (A), reticuline (B), coclaurine (C), higenamine (D), linderane (E), isolinderalactone (F), and linderone (G).

Article Snippet: Boldine (P/N, X23N9Y73113), isolinderalactone (P/N, S30HB196732), reticuline (P/N, N28HB202502), linderone (P/N, D0HB03066), and methyllinderone (P/N, D0HB03065) were purchase from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China).

Techniques: Tandem Mass Spectroscopy

Journal: RSC Advances

Article Title: Characterization and quantification of the phytochemical constituents and anti-inflammatory properties of Lindera aggregata †

doi: 10.1039/d4ra05643d

Figure Lengend Snippet: Validation results of the developed quantification method of 16 compounds ( n = 6)

Article Snippet: Boldine (P/N, X23N9Y73113), isolinderalactone (P/N, S30HB196732), reticuline (P/N, N28HB202502), linderone (P/N, D0HB03066), and methyllinderone (P/N, D0HB03065) were purchase from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China).

Techniques: Biomarker Discovery