reg3γ  (ATCC)

Journal: Immunologic Research

Article Title: Baicalin restores innate lymphoid immune imbalance during exacerbation of COPD

doi: 10.1007/s12026-025-09629-2

Figure Lengend Snippet: Primer sequences

Article Snippet: Reg3γ antibody (A2146) and Recombinant mouse IL- 22 (RP02942) were purchased from ABclonal Technology Co.,Ltd. (Wuhan, Hubei, China).

Techniques:

Journal: Immunologic Research

Article Title: Baicalin restores innate lymphoid immune imbalance during exacerbation of COPD

doi: 10.1007/s12026-025-09629-2

Figure Lengend Snippet: CS and LPS-induced ECOPD mice show an upregulation of IL- 22/Reg3γ axis. a Expression of IL- 22 in both protein and mRNA levels in BALF and lung tissues. b Expression of IL- 22, GATA3, and Reg3γ in lung tissues, assessed using immuohistochemical staining. c Expression of IL- 22, GATA3, and Reg3γ in lung tissues, assessed using western blotting. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. LTH: lung tissue homogenate

Article Snippet: Reg3γ antibody (A2146) and Recombinant mouse IL- 22 (RP02942) were purchased from ABclonal Technology Co.,Ltd. (Wuhan, Hubei, China).

Techniques: Expressing, Staining, Western Blot

Journal: Immunologic Research

Article Title: Baicalin restores innate lymphoid immune imbalance during exacerbation of COPD

doi: 10.1007/s12026-025-09629-2

Figure Lengend Snippet: Baicalin inhibits the IL- 22/Reg3γ axis in ECOPD mice. a The effect of baicalin on the expression of IL- 22 in lung tissue in ECOPD mice, assessed using ELISA and qPCR. b The effect of baicalin on the expression of IL- 22, GATA3, and Reg3γ in lung tissue in ECOPD mice, assessed using immuohistochemical staining. c The effect of baicalin on the expression of IL- 22 and Reg3γ in lung tissue in ECOPD mice, assessed using western blotting. d The effect of baicalin on the number of IL- 22-producing ILC3 s in lung tissue in ECOPD mice. e The effect of baicalin on the production of IL- 22 in isolated human blood ILC3 s. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. BA: baicalin; LTH: lung tissue homogenate

Article Snippet: Reg3γ antibody (A2146) and Recombinant mouse IL- 22 (RP02942) were purchased from ABclonal Technology Co.,Ltd. (Wuhan, Hubei, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Staining, Western Blot, Isolation

Journal: Gut microbes

Article Title: Intestinal epithelial pH-sensing receptor GPR65 maintains mucosal homeostasis via regulating antimicrobial defense and restrains gut inflammation in inflammatory bowel disease.

doi: 10.1080/19490976.2023.2257269

Figure Lengend Snippet: Figure 1. IEC-derived GPR65 preserves intestinal antimicrobial activity. (a-c) qPCR analysis of the indicated genes in IECs from the large bowels of Gpr65fl/fl and Gpr65ΔIEC mice. (d-f) qPCR analysis of the indicated genes in IECs from the small bowels of Gpr65fl/fl and Gpr65ΔIEC mice. (g) representative immunofluorescence staining of REG3γ in both large and small bowels from Gpr65fl/fl and Gpr65ΔIEC

Article Snippet: Primary antibodies against REG3γ (Cat: ab198216), β-actin (Cat: sc-8432), and GPR65 (Cat: PA5–111835) were purchased from Abcam (Cambridge, UK), Santa Cruz Biotechnology (Dallas, TX, USA), and Thermo Fisher Scientific, respectively.

Techniques: Derivative Assay, Activity Assay, Immunofluorescence, Staining