c glutamicum atcc 13032 reference genome  (ATCC)

Journal: Synthetic and Systems Biotechnology

Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress

doi: 10.1016/j.synbio.2026.01.020

Figure Lengend Snippet: Improving the tolerance to formaldehyde via ALE. (A) Growth of FM-1 in minimal medium with 10 g/L glucose and different formaldehyde concentrations. 0 mM (square), 0.5 mM (triangle), 0.8 mM (circle), and 1 mM (inverted triangle). (B) ALE procedure of culture-1 in CGXII minimal medium supplemented with different formaldehyde concentrations and 10 g/L glucose. (C) Growth curve of the evolved mutants in CGXII minimal medium supplemented with 10 g/L glucose and 0.8 mM formaldehyde. (D) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose and 1.6 mM formaldehyde. (E) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose. (F) Formaldehyde degradation during cell growth of wild-type C. glutamicum ATCC 13032, FM-1 and FM-3. Values and error bars reflect the mean ± s.d. of three biological replicates (N = 3).

Article Snippet: Each evolved strain possesses more than 100 mutations including single nucleotide polymorphisms (SNPs), insertions, and deletions, which were aligned against the C. glutamicum ATCC 13032 reference genome (GenBank accession number GCA_000011325.1).

Techniques: Mutagenesis

Journal: International Dental Journal

Article Title: Immunological Features of Peri-Implant Soft Tissue After Healing Abutment Dislodgement: A Comparative Human Study

doi: 10.1016/j.identj.2026.109521

Figure Lengend Snippet: Immunohistochemical staining for immune cell markers in the epithelial + superficial connective tissue region. Representative immunohistochemical images of the superficial peri-implant soft tissue zone stained for MPO, CD68, CD3, CD20, and CD138. Scale bars: 100 μm. Red dashed boxes and red arrows indicate the magnified regions (insets).

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies (MPO, CD68, CD3, CD20 and CD138, all purchased from Boster, China; dilution 1:400).

Techniques: Immunohistochemical staining, Staining