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proteintech 22280 1 ap rbms1 (Proteintech)

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Proteintech proteintech 22280 1 ap rbms1
Proteintech 22280 1 Ap Rbms1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteintech 22280 1 ap rbms1 - by Bioz Stars, 2026-03

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( A ) Transcriptional expression of RBMS1 in RNA-seq data ( GSE135055 ) including Control ( n = 9) and HF patients ( n = 21). Control (minimum: 0.072, Q1: 0.075, median: 0.08, Q3: 0.083, maximum: 0.087), HF (minimum: 0.069, Q1: 0.095, median: 0.1, Q3: 0.108, maximum: 0.128). ( B ) Pearson correlation analysis of the expression of RBMS1 with NPPA and NPPB in RNA-seq data ( GSE135055 ). ( C ) Transcriptional expression of RBMS1 in snRNA-seq data (SCP1303) ( n = 16). Control (minimum: 0, Q1: 1.045, median: 1.42, Q3: 1.90, maximum: 4.23), HCM (minimum: 0, Q1: 1.33, median: 1.69, Q3: 2.12, maximum: 4.59). ( D , E ) Western blot and qRT-PCR showing the protein and mRNA expression of RBMS1 in Control and DCM patients ( n = 5). ( F ) Quantification of mRNA levels of RBMS1, MYH7, NPPA, and NPPB in Sham and TAC mice ( n = 6). ( G ) Western blotting and quantification showing the protein expression of β-MHC and RBMS1 ( n = 5). ( H ) Schematic diagram showing the isolation of cardiomyocytes from Ang II and TAC mice. ( I , J ) Quantification of mRNA levels of RBMS1 in cardiomyocytes isolated from Ang II and TAC mice ( n = 6). ( K ) Quantification of mRNA levels of RBMS1, MYH7, NPPA, and NPPB in NMCMs induced by Ang II ( n = 5). ( L ) Western blotting showing protein expression levels of β-MHC, RBMS1, and ANP ( n = 6). ( M ) Quantification of mRNA levels of RBMS1, MYH7, NPPA, and NPPB in NMCMs induced by ISO ( n = 5). ( N ) Western blotting showing protein expression levels of β-MHC, RBMS1, and ANP ( n = 5). Data information: data are shown as mean ± SEM, P values were analyzed with unpaired Student’s t test ( A , C , D , E , F for MYH7, NPPA, and NPPB, G , I , J , K for RBMS1, MYH7, and NPPA, L , M for MYH7 and NPPB, N ) and Mann–Whitney U test ( F for RBMS1, K for NPPB, M for RBMS1 and NPPA). 2.2e-16 is the threshold commonly used in R language output, indicating a highly statistically significant result ( C ). A dot represents an independent biological sample. .

Journal: EMBO Molecular Medicine

Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics

doi: 10.1038/s44321-025-00334-z

Figure Lengend Snippet: ( A ) Transcriptional expression of RBMS1 in RNA-seq data ( GSE135055 ) including Control ( n = 9) and HF patients ( n = 21). Control (minimum: 0.072, Q1: 0.075, median: 0.08, Q3: 0.083, maximum: 0.087), HF (minimum: 0.069, Q1: 0.095, median: 0.1, Q3: 0.108, maximum: 0.128). ( B ) Pearson correlation analysis of the expression of RBMS1 with NPPA and NPPB in RNA-seq data ( GSE135055 ). ( C ) Transcriptional expression of RBMS1 in snRNA-seq data (SCP1303) ( n = 16). Control (minimum: 0, Q1: 1.045, median: 1.42, Q3: 1.90, maximum: 4.23), HCM (minimum: 0, Q1: 1.33, median: 1.69, Q3: 2.12, maximum: 4.59). ( D , E ) Western blot and qRT-PCR showing the protein and mRNA expression of RBMS1 in Control and DCM patients ( n = 5). ( F ) Quantification of mRNA levels of RBMS1, MYH7, NPPA, and NPPB in Sham and TAC mice ( n = 6). ( G ) Western blotting and quantification showing the protein expression of β-MHC and RBMS1 ( n = 5). ( H ) Schematic diagram showing the isolation of cardiomyocytes from Ang II and TAC mice. ( I , J ) Quantification of mRNA levels of RBMS1 in cardiomyocytes isolated from Ang II and TAC mice ( n = 6). ( K ) Quantification of mRNA levels of RBMS1, MYH7, NPPA, and NPPB in NMCMs induced by Ang II ( n = 5). ( L ) Western blotting showing protein expression levels of β-MHC, RBMS1, and ANP ( n = 6). ( M ) Quantification of mRNA levels of RBMS1, MYH7, NPPA, and NPPB in NMCMs induced by ISO ( n = 5). ( N ) Western blotting showing protein expression levels of β-MHC, RBMS1, and ANP ( n = 5). Data information: data are shown as mean ± SEM, P values were analyzed with unpaired Student’s t test ( A , C , D , E , F for MYH7, NPPA, and NPPB, G , I , J , K for RBMS1, MYH7, and NPPA, L , M for MYH7 and NPPB, N ) and Mann–Whitney U test ( F for RBMS1, K for NPPB, M for RBMS1 and NPPA). 2.2e-16 is the threshold commonly used in R language output, indicating a highly statistically significant result ( C ). A dot represents an independent biological sample. .

Article Snippet: RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

Techniques: Expressing, RNA Sequencing, Control, Western Blot, Quantitative RT-PCR, Isolation, MANN-WHITNEY

( A ) Experimental protocol of mice with AAV9 injection and TAC surgery, C57BL/6J mice were administered AAV9-RBMS1 via tail vein injection at a concentration of 5 × 10 11 vg/mL. ( B and C ) Western blot and qRT-PCR showing protein and mRNA levels of RBMS1 in cardiomyocytes isolated from AAV9-RBMS1 mice ( n = 4). ( D ) Representative echocardiography of WT mice injected with AAV9-Vector or AAV9-RBMS1 for 1 week and subsequently subjected to sham or TAC surgery for 8 weeks. ( E ) Quantification of LVEF ( n = 14). ( F ) Heart size and histological sections of heart samples were stained with Masson staining, WGA staining, and H&E staining. For heart size, scale bar = 5 mm; for cross-section of the heart, scale bar = 1 mm; for Masson, WGA, and H&E staining, scale bar = 50 μm. ( G – I ) HW/BW, HW/TL ( n = 14), perivascular fibrotic area ( n = 6), and cross-sectional area of cardiomyocytes ( n = 70) were examined. ( J , K ) Western blotting and quantification showing protein levels of β-MHC and ANP ( n = 5). ( L ) Quantification of mRNA levels of MYH7, NPPA, and NPPB ( n = 5). ( M ) Representative immunofluorescence staining of α-actinin and quantification in NMCMs transfected with Ad-RBMS1 in response to Ang II ( n = 6). Scale bar = 50 μm. Data information: data are shown as mean ± SEM, P values were analyzed with unpaired Student’s t test ( B , C ) and one-way ANOVA test ( E , G , H , I , K , L , M ). A dot represents an independent biological sample. .

Journal: EMBO Molecular Medicine

Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics

doi: 10.1038/s44321-025-00334-z

Figure Lengend Snippet: ( A ) Experimental protocol of mice with AAV9 injection and TAC surgery, C57BL/6J mice were administered AAV9-RBMS1 via tail vein injection at a concentration of 5 × 10 11 vg/mL. ( B and C ) Western blot and qRT-PCR showing protein and mRNA levels of RBMS1 in cardiomyocytes isolated from AAV9-RBMS1 mice ( n = 4). ( D ) Representative echocardiography of WT mice injected with AAV9-Vector or AAV9-RBMS1 for 1 week and subsequently subjected to sham or TAC surgery for 8 weeks. ( E ) Quantification of LVEF ( n = 14). ( F ) Heart size and histological sections of heart samples were stained with Masson staining, WGA staining, and H&E staining. For heart size, scale bar = 5 mm; for cross-section of the heart, scale bar = 1 mm; for Masson, WGA, and H&E staining, scale bar = 50 μm. ( G – I ) HW/BW, HW/TL ( n = 14), perivascular fibrotic area ( n = 6), and cross-sectional area of cardiomyocytes ( n = 70) were examined. ( J , K ) Western blotting and quantification showing protein levels of β-MHC and ANP ( n = 5). ( L ) Quantification of mRNA levels of MYH7, NPPA, and NPPB ( n = 5). ( M ) Representative immunofluorescence staining of α-actinin and quantification in NMCMs transfected with Ad-RBMS1 in response to Ang II ( n = 6). Scale bar = 50 μm. Data information: data are shown as mean ± SEM, P values were analyzed with unpaired Student’s t test ( B , C ) and one-way ANOVA test ( E , G , H , I , K , L , M ). A dot represents an independent biological sample. .

Article Snippet: RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

Techniques: Injection, Concentration Assay, Western Blot, Quantitative RT-PCR, Isolation, Plasmid Preparation, Staining, Immunofluorescence, Transfection

( A ) Cardiomyocyte-specific RBMS1 knockout (RBMS1-cKO) mice were reared until 8 weeks of age, subjected to either sham or TAC surgery, and then maintained for an additional 8 weeks until tissue harvest. RBMS1-cKO mice were generated by crossing RBMS1 flox/flox mice with MYH6-Cre mice. ( B ) Representative echocardiography of control and RBMS1-cKO mice with sham or TAC surgery. ( C ) Quantification of LVEF ( n = 10). ( D ) Heart size and histological sections of heart samples were stained with Masson, WGA, and H&E staining. For heart size, scale bar = 5 mm; for cross section of the heart, scale bar = 1 mm; for Masson, WGA, and H&E staining, scale bar = 50 μm. ( E – G ) HW/BW, HW/TL ( n = 10), perivascular fibrotic area ( n = 6 mice), and cross sectional area of cardiomyocytes ( n = 50) were examined. ( H , I ) Western blotting and quantification showing protein levels of β-MHC and ANP ( n = 6). ( J ) Quantification of mRNA levels of MYH7, NPPA, and NPPB ( n = 6). ( K ) Representative immunofluorescence staining of α-actinin and quantification in NMCMs transfected with si-RBMS1 and subsequently treated with Ang II ( n = 6). Scale bar = 50 μm. Data information: data are shown as mean ± SEM, P values were analyzed with one-way ANOVA test ( C , E , F , I , J for NPPA and NPPB, K ) and Kruskal–Wallis test ( G , J for MYH7). A dot represents an independent biological sample. .

Journal: EMBO Molecular Medicine

Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics

doi: 10.1038/s44321-025-00334-z

Figure Lengend Snippet: ( A ) Cardiomyocyte-specific RBMS1 knockout (RBMS1-cKO) mice were reared until 8 weeks of age, subjected to either sham or TAC surgery, and then maintained for an additional 8 weeks until tissue harvest. RBMS1-cKO mice were generated by crossing RBMS1 flox/flox mice with MYH6-Cre mice. ( B ) Representative echocardiography of control and RBMS1-cKO mice with sham or TAC surgery. ( C ) Quantification of LVEF ( n = 10). ( D ) Heart size and histological sections of heart samples were stained with Masson, WGA, and H&E staining. For heart size, scale bar = 5 mm; for cross section of the heart, scale bar = 1 mm; for Masson, WGA, and H&E staining, scale bar = 50 μm. ( E – G ) HW/BW, HW/TL ( n = 10), perivascular fibrotic area ( n = 6 mice), and cross sectional area of cardiomyocytes ( n = 50) were examined. ( H , I ) Western blotting and quantification showing protein levels of β-MHC and ANP ( n = 6). ( J ) Quantification of mRNA levels of MYH7, NPPA, and NPPB ( n = 6). ( K ) Representative immunofluorescence staining of α-actinin and quantification in NMCMs transfected with si-RBMS1 and subsequently treated with Ang II ( n = 6). Scale bar = 50 μm. Data information: data are shown as mean ± SEM, P values were analyzed with one-way ANOVA test ( C , E , F , I , J for NPPA and NPPB, K ) and Kruskal–Wallis test ( G , J for MYH7). A dot represents an independent biological sample. .

Article Snippet: RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

Techniques: Knock-Out, Generated, Control, Staining, Western Blot, Immunofluorescence, Transfection

( A , B ) Western blotting and quantification showing protein levels of β-MHC, RBMS1, and ANP in hiPSC-CMs transfected with RBMS1 in response to Ang II stimulation ( n = 3). ( C ) Quantification of mRNA levels of RBMS1, MYH7, NPPA, and NPPB ( n = 6). ( D ) Representative immunofluorescence staining of α-actinin and quantification in hiPSC-CMs transfected with RBMS1 ( n = 6). Scale bar = 50 μm. ( E ) Quantification of mRNA levels of RBMS1 in hiPSC-CMs transfected with si-NC or si-RBMS1 ( n = 3). ( F ) Representative immunofluorescence staining of α-actinin and quantification in hiPSC-CMs transfected with si-RBMS1 ( n = 6). Scale bar = 50 μm. Data information: data are shown as mean ± SEM, P values were analyzed with unpaired Student’s t test ( E ) and one-way ANOVA test ( B , C , D , F ). A dot represents an independent biological sample. .

Journal: EMBO Molecular Medicine

Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics

doi: 10.1038/s44321-025-00334-z

Figure Lengend Snippet: ( A , B ) Western blotting and quantification showing protein levels of β-MHC, RBMS1, and ANP in hiPSC-CMs transfected with RBMS1 in response to Ang II stimulation ( n = 3). ( C ) Quantification of mRNA levels of RBMS1, MYH7, NPPA, and NPPB ( n = 6). ( D ) Representative immunofluorescence staining of α-actinin and quantification in hiPSC-CMs transfected with RBMS1 ( n = 6). Scale bar = 50 μm. ( E ) Quantification of mRNA levels of RBMS1 in hiPSC-CMs transfected with si-NC or si-RBMS1 ( n = 3). ( F ) Representative immunofluorescence staining of α-actinin and quantification in hiPSC-CMs transfected with si-RBMS1 ( n = 6). Scale bar = 50 μm. Data information: data are shown as mean ± SEM, P values were analyzed with unpaired Student’s t test ( E ) and one-way ANOVA test ( B , C , D , F ). A dot represents an independent biological sample. .

Article Snippet: RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

Techniques: Western Blot, Transfection, Immunofluorescence, Staining

( A ) Representative immunofluorescence staining of RBMS1. Scale bar = 20 μm. ( B ) Percent of AS types in NMCMs transfected with Ad-RBMS1 ( n = 3). Skipping Exon (SE), Alternative 3′ Splice Site (A3SS), Mutually Exclusive Exons (MXE), Retained intron (RI), Alternative 5′ Splice Site (A5SS). ( C ) GO analysis of differential splicing genes. ( D ) Transmission electron microscope showed the disorganization of sarcomeres in RBMS1 overexpression mice treatment with TAC surgery ( n = 4), scale bar = 1 and 2 μm. The red and yellow arrows respectively represent the Z-disc and M-band. ( E ) Immunofluorescence of α-Actinin and ACTN2 in RBMS1 overexpression mice, scale bar = 20 μm. ( F ) Transmission electron microscope showed the disorganization of sarcomeres in RBMS1-cKO mice, scale bar = 1 and 2 μm. The red and yellow arrows respectively represent the Z-disc and M-band. ( G ) Immunofluorescence of α-Actinin and ACTN2 in RBMS1-cKO mice, scale bar=20 μm. ( H ) Immunofluorescence staining of ACTN2 and phalloidine showed the sarcomere and cytoskeleton of hiPSC-CMs and quantification of sarcomere length and representative polarity histogram of sarcomere organization ( n = 70), scale bar = 20 μm. ( I ) Immunofluorescence staining of ACTN2 and phalloidine showed the sarcomere and cytoskeleton of hiPSC-CMs and quantification of sarcomere length and representative polarity histogram of sarcomere organization ( n = 70), scale bar = 20 μm. Data information: data are shown as mean ± SEM, P values were analyzed with one-way ANOVA test ( H , I ), polarity histograms were generated by MATLAB R2024b ( H , I ). .

Journal: EMBO Molecular Medicine

Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics

doi: 10.1038/s44321-025-00334-z

Figure Lengend Snippet: ( A ) Representative immunofluorescence staining of RBMS1. Scale bar = 20 μm. ( B ) Percent of AS types in NMCMs transfected with Ad-RBMS1 ( n = 3). Skipping Exon (SE), Alternative 3′ Splice Site (A3SS), Mutually Exclusive Exons (MXE), Retained intron (RI), Alternative 5′ Splice Site (A5SS). ( C ) GO analysis of differential splicing genes. ( D ) Transmission electron microscope showed the disorganization of sarcomeres in RBMS1 overexpression mice treatment with TAC surgery ( n = 4), scale bar = 1 and 2 μm. The red and yellow arrows respectively represent the Z-disc and M-band. ( E ) Immunofluorescence of α-Actinin and ACTN2 in RBMS1 overexpression mice, scale bar = 20 μm. ( F ) Transmission electron microscope showed the disorganization of sarcomeres in RBMS1-cKO mice, scale bar = 1 and 2 μm. The red and yellow arrows respectively represent the Z-disc and M-band. ( G ) Immunofluorescence of α-Actinin and ACTN2 in RBMS1-cKO mice, scale bar=20 μm. ( H ) Immunofluorescence staining of ACTN2 and phalloidine showed the sarcomere and cytoskeleton of hiPSC-CMs and quantification of sarcomere length and representative polarity histogram of sarcomere organization ( n = 70), scale bar = 20 μm. ( I ) Immunofluorescence staining of ACTN2 and phalloidine showed the sarcomere and cytoskeleton of hiPSC-CMs and quantification of sarcomere length and representative polarity histogram of sarcomere organization ( n = 70), scale bar = 20 μm. Data information: data are shown as mean ± SEM, P values were analyzed with one-way ANOVA test ( H , I ), polarity histograms were generated by MATLAB R2024b ( H , I ). .

Article Snippet: RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

Techniques: Immunofluorescence, Staining, Transfection, Transmission Assay, Microscopy, Over Expression, Generated

( A ) Quantification of sarcomere length and representative polarity histogram of sarcomere organization in RBMS1 overexpression mice treatment with TAC surgery ( n = 70). ( B ) Quantification of sarcomere length and representative polarity histogram of sarcomere organization in RBMS1-cKO mice treatment with TAC surgery ( n = 70). ( C , D ) Quantification of sarcomere length and representative polarity histogram of sarcomere organization in NMCMs transfected with Ad-RBMS1 in response to Ang II stimulation ( n = 70). ( E , F ) Quantification of sarcomere length and representative polarity histogram of sarcomere organization in NMCMs transfected with si-RBMS1 and subsequently treated with Ang II ( n = 70). Data information: data are shown as mean ± SEM, P values were analyzed with one-way ANOVA test ( A , B , D , F ), polarity histograms were generated by MATLAB R2024b ( A , B , D , F ). .

Journal: EMBO Molecular Medicine

Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics

doi: 10.1038/s44321-025-00334-z

Figure Lengend Snippet: ( A ) Quantification of sarcomere length and representative polarity histogram of sarcomere organization in RBMS1 overexpression mice treatment with TAC surgery ( n = 70). ( B ) Quantification of sarcomere length and representative polarity histogram of sarcomere organization in RBMS1-cKO mice treatment with TAC surgery ( n = 70). ( C , D ) Quantification of sarcomere length and representative polarity histogram of sarcomere organization in NMCMs transfected with Ad-RBMS1 in response to Ang II stimulation ( n = 70). ( E , F ) Quantification of sarcomere length and representative polarity histogram of sarcomere organization in NMCMs transfected with si-RBMS1 and subsequently treated with Ang II ( n = 70). Data information: data are shown as mean ± SEM, P values were analyzed with one-way ANOVA test ( A , B , D , F ), polarity histograms were generated by MATLAB R2024b ( A , B , D , F ). .

Article Snippet: RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

Techniques: Over Expression, Transfection, Generated

( A ) Volcanic plot elucidated the differential splicing genes. ( B ) Schematic diagrams showed the detailed information of differential splicing genes. ( C ) Splicing pattern and quantification of CTTN in NMCMs transfected with Ad-RBMS1 ( n = 5). ( D ) snRNA-seq dataset (SCP1303) analysis of the distribution of CTTN expression in various cardiac cells. ( E ) Splicing pattern and quantification of CTTN in sham and TAC mice ( n = 3). ( F ) Splicing pattern and quantification of CTTN in NMCMs treatment with Ang II ( n = 4). ( G ) Splicing pattern and quantification of CTTN in Control and DCM patients ( n = 5). ( H ) Pearson correlation analysis of the expression of CTTN-Δe11 with RBMS1 in DCM patients. ( I and J ) RNA pull-down and RIP assay showed the binding of RBMS1 protein and CTTN mRNA in NMCMs. ( K ) Left, Schematic diagram of minigene with the exon 10 to exon 12 of CTTN. Right, the splicing pattern and quantification of CTTN ( n = 3). ( L ) Left, Schematic diagram of CTTN mutation minigene with RBMS1-binding sites. Right, the splicing pattern and quantification of CTTN ( n = 3). Data information: data are shown as mean ± SEM, P values were analyzed with unpaired Student’s t test ( C , E , F , G ) and two-way ANOVA test ( K , L ). A dot represents an independent biological sample. .

Journal: EMBO Molecular Medicine

Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics

doi: 10.1038/s44321-025-00334-z

Figure Lengend Snippet: ( A ) Volcanic plot elucidated the differential splicing genes. ( B ) Schematic diagrams showed the detailed information of differential splicing genes. ( C ) Splicing pattern and quantification of CTTN in NMCMs transfected with Ad-RBMS1 ( n = 5). ( D ) snRNA-seq dataset (SCP1303) analysis of the distribution of CTTN expression in various cardiac cells. ( E ) Splicing pattern and quantification of CTTN in sham and TAC mice ( n = 3). ( F ) Splicing pattern and quantification of CTTN in NMCMs treatment with Ang II ( n = 4). ( G ) Splicing pattern and quantification of CTTN in Control and DCM patients ( n = 5). ( H ) Pearson correlation analysis of the expression of CTTN-Δe11 with RBMS1 in DCM patients. ( I and J ) RNA pull-down and RIP assay showed the binding of RBMS1 protein and CTTN mRNA in NMCMs. ( K ) Left, Schematic diagram of minigene with the exon 10 to exon 12 of CTTN. Right, the splicing pattern and quantification of CTTN ( n = 3). ( L ) Left, Schematic diagram of CTTN mutation minigene with RBMS1-binding sites. Right, the splicing pattern and quantification of CTTN ( n = 3). Data information: data are shown as mean ± SEM, P values were analyzed with unpaired Student’s t test ( C , E , F , G ) and two-way ANOVA test ( K , L ). A dot represents an independent biological sample. .

Article Snippet: RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

Techniques: Transfection, Expressing, Control, Binding Assay, Mutagenesis

( A ) Experimental protocol of mice with AAV9 injection and TAC surgery. C57BL/6J mice were administered AAV9-RBMS1 (5 × 10 11 vg/mL) and AAV9-sh-CTTN-Δe11 (1 × 10 11 vg/mL) via tail vein injection. ( B ) Representative echocardiography of different group mice. ( C ) Quantification of LVEF ( n = 12). ( D ) Heart size and histological sections of heart samples were stained with Masson and WGA staining. For heart size, scale bar = 5 mm; for cross-section of the heart, scale bar = 1 mm; for Masson and WGA staining, scale bar = 50 μm. ( E – G ) HW/BW, HW/TL ( n = 12), perivascular fibrotic area ( n = 6), and cross-sectional area of cardiomyocytes ( n = 70) were examined in different groups. ( H , I ) Western blotting and quantification showing protein levels of β-MHC and ANP ( n = 6). ( J ) Transmission electron microscope showed the disorganization of sarcomeres ( n = 4), scale bar = 1 and 2 μm. The red and yellow arrows respectively represent the Z-disc and M-band. ( K ) Immunofluorescence showed the disorganization of sarcomeres ( n = 6), scale bar = 20 μm. Data information: data are shown as mean ± SEM, P values were analyzed with one-way ANOVA test ( C , E , F , G , I ). A dot represents an independent biological sample. .

Journal: EMBO Molecular Medicine

Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics

doi: 10.1038/s44321-025-00334-z

Figure Lengend Snippet: ( A ) Experimental protocol of mice with AAV9 injection and TAC surgery. C57BL/6J mice were administered AAV9-RBMS1 (5 × 10 11 vg/mL) and AAV9-sh-CTTN-Δe11 (1 × 10 11 vg/mL) via tail vein injection. ( B ) Representative echocardiography of different group mice. ( C ) Quantification of LVEF ( n = 12). ( D ) Heart size and histological sections of heart samples were stained with Masson and WGA staining. For heart size, scale bar = 5 mm; for cross-section of the heart, scale bar = 1 mm; for Masson and WGA staining, scale bar = 50 μm. ( E – G ) HW/BW, HW/TL ( n = 12), perivascular fibrotic area ( n = 6), and cross-sectional area of cardiomyocytes ( n = 70) were examined in different groups. ( H , I ) Western blotting and quantification showing protein levels of β-MHC and ANP ( n = 6). ( J ) Transmission electron microscope showed the disorganization of sarcomeres ( n = 4), scale bar = 1 and 2 μm. The red and yellow arrows respectively represent the Z-disc and M-band. ( K ) Immunofluorescence showed the disorganization of sarcomeres ( n = 6), scale bar = 20 μm. Data information: data are shown as mean ± SEM, P values were analyzed with one-way ANOVA test ( C , E , F , G , I ). A dot represents an independent biological sample. .

Article Snippet: RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

Techniques: Injection, Staining, Western Blot, Transmission Assay, Microscopy, Immunofluorescence

( A ) Western blotting and quantification showing protein levels of β-MHC and ANP in RBMS1 overexpression NMCMs transfected with si-Δe11 in response to Ang II stimulation ( n = 6). ( B ) Quantification of mRNA levels of MYH7, NPPA, and NPPB ( n = 6). ( C , D ) Representative immunofluorescence staining of α-actinin and quantification of cell surface area ( n = 8), scale bar = 50 μm. ( E ) Immunofluorescence staining of ACTN2 and phalloidine showed the disorganization of sarcomere and cytoskeleton in NMCMs, scale bar = 20 μm. ( F , G ) Quantification of sarcomere length and representative polarity histogram of sarcomere organization in NMCMs ( n = 70). Data information: data are shown as mean ± SEM, P values were analyzed with one-way ANOVA test ( A , B , D , F ), polarity histograms were generated by MATLAB R2024b ( G ). .

Journal: EMBO Molecular Medicine

Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics

doi: 10.1038/s44321-025-00334-z

Figure Lengend Snippet: ( A ) Western blotting and quantification showing protein levels of β-MHC and ANP in RBMS1 overexpression NMCMs transfected with si-Δe11 in response to Ang II stimulation ( n = 6). ( B ) Quantification of mRNA levels of MYH7, NPPA, and NPPB ( n = 6). ( C , D ) Representative immunofluorescence staining of α-actinin and quantification of cell surface area ( n = 8), scale bar = 50 μm. ( E ) Immunofluorescence staining of ACTN2 and phalloidine showed the disorganization of sarcomere and cytoskeleton in NMCMs, scale bar = 20 μm. ( F , G ) Quantification of sarcomere length and representative polarity histogram of sarcomere organization in NMCMs ( n = 70). Data information: data are shown as mean ± SEM, P values were analyzed with one-way ANOVA test ( A , B , D , F ), polarity histograms were generated by MATLAB R2024b ( G ). .

Article Snippet: RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

Techniques: Western Blot, Over Expression, Transfection, Immunofluorescence, Staining, Generated

( A ) Division of cardiomyocytes with high and low expression of RBMS1 in snRNA-seq dataset (SCP1303). ( B ) Functional enrichment analysis of cardiomyocytes with high expression of RBMS1. ( C ) The score of PI3K/AKT in cardiomyocytes with high expression of RBMS1. RBMS1 - (minimum: −0.037, Q1: 0.044, median: 0.061, Q3: 0.077, maximum: 0.214), RBMS1 + (minimum: −0.02, Q1: 0.063, median: 0.078, Q3: 0.093, maximum: 0.211). ( D ) Western blotting and quantification showing protein levels of p-PI3K and p-AKT in AAV9-RBMS1 mice ( n = 6). ( E ) Western blotting and quantification showing protein levels of p-PI3K and p-AKT in RBMS1-cKO mice with TAC surgery ( n = 6). ( F ) Results of KEGG enrichment analysis based on differentially expressed differential splicing genes. ( G ) Western blotting and quantification showing protein levels of p-PI3K and p-AKT in CTTN-Δe11 knockdown mice with TAC surgery ( n = 6). ( H ) Western blotting and quantification showing protein levels of P100α and P100γ in RBMS1 overexpression NMCMs treatment with Alpelisib in response to Ang II stimulation ( n = 4). ( I ) Western blotting and quantification showing protein levels of p-PI3K and p-AKT in Ad-RBMS1 NMCMs treatment with Alpelisib ( n = 6). ( J ) Immunofluorescence staining of ACTN2 and phalloidine showed the disorganization of sarcomere and cytoskeleton, scale bar = 20 μm. Data information: data are shown as mean ± SEM, P values were analyzed with unpaired Student’s t test ( C , D ) and one-way ANOVA test ( E , G , H , I ). 2.2e-16 is the threshold commonly used in R language output, indicating a highly statistically significant result ( C ). A dot represents an independent biological sample. .

Journal: EMBO Molecular Medicine

Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics

doi: 10.1038/s44321-025-00334-z

Figure Lengend Snippet: ( A ) Division of cardiomyocytes with high and low expression of RBMS1 in snRNA-seq dataset (SCP1303). ( B ) Functional enrichment analysis of cardiomyocytes with high expression of RBMS1. ( C ) The score of PI3K/AKT in cardiomyocytes with high expression of RBMS1. RBMS1 - (minimum: −0.037, Q1: 0.044, median: 0.061, Q3: 0.077, maximum: 0.214), RBMS1 + (minimum: −0.02, Q1: 0.063, median: 0.078, Q3: 0.093, maximum: 0.211). ( D ) Western blotting and quantification showing protein levels of p-PI3K and p-AKT in AAV9-RBMS1 mice ( n = 6). ( E ) Western blotting and quantification showing protein levels of p-PI3K and p-AKT in RBMS1-cKO mice with TAC surgery ( n = 6). ( F ) Results of KEGG enrichment analysis based on differentially expressed differential splicing genes. ( G ) Western blotting and quantification showing protein levels of p-PI3K and p-AKT in CTTN-Δe11 knockdown mice with TAC surgery ( n = 6). ( H ) Western blotting and quantification showing protein levels of P100α and P100γ in RBMS1 overexpression NMCMs treatment with Alpelisib in response to Ang II stimulation ( n = 4). ( I ) Western blotting and quantification showing protein levels of p-PI3K and p-AKT in Ad-RBMS1 NMCMs treatment with Alpelisib ( n = 6). ( J ) Immunofluorescence staining of ACTN2 and phalloidine showed the disorganization of sarcomere and cytoskeleton, scale bar = 20 μm. Data information: data are shown as mean ± SEM, P values were analyzed with unpaired Student’s t test ( C , D ) and one-way ANOVA test ( E , G , H , I ). 2.2e-16 is the threshold commonly used in R language output, indicating a highly statistically significant result ( C ). A dot represents an independent biological sample. .

Article Snippet: RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

Techniques: Expressing, Functional Assay, Western Blot, Knockdown, Over Expression, Immunofluorescence, Staining

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