rapa  (MedChemExpress)

Journal: iScience

Article Title: KNSTRN knockdown impairs autophagy flux to inhibit bladder cancer progression

doi: 10.1016/j.isci.2026.114734

Figure Lengend Snippet: KNSTRN knockdown suppresses autophagic flux in BLCA cells (A and B) T24 and 5637 cells were first transfected with mCherry-GFP-LC3 for 24 h and subsequently transfected with either si-NC or si-KNSTRN for an additional 48 h. The number of LC3 puncta (yellow for autophagosomes, red for autolysosomes) was quantified. And images were captured using a fluorescence microscope. Scale bars, 20 μm. (C) Western blot was used to determine the protein expression levels of P62. β-actin served as a control. KNSTRN-silenced and control cells were treated with either DMSO or rapamycin (Rapa, T24, 20 nM; 5637, and 50 nM) for 24 h. (D) Western blot was used to determine the protein expression levels of LC3. β-actin served as a control. KNSTRN-silenced and control cells were treated with either DMSO or chloroquine (CQ, T24, 50 μM; 5637, and 100 μM) for 24 h. Significance is determined by one-way ANOVA. Data are presented as the mean ± SD ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significantly different.

Article Snippet: Rapamycin (Rapa) , Selleck , Cat# S1039.

Techniques: Knockdown, Transfection, Fluorescence, Microscopy, Western Blot, Expressing, Control

Journal: iScience

Article Title: KNSTRN knockdown impairs autophagy flux to inhibit bladder cancer progression

doi: 10.1016/j.isci.2026.114734

Figure Lengend Snippet: Rapamycin inhibits KNSTRN knockdown-induced cell death in vitro (A and B) CCK-8 and plate colony formation assays in T24 and 5637 cells. KNSTRN-silenced and si-NC cells were treated with either DMSO or rapamycin (Rapa, T24, 20 nM; 5637, 50 nM) for 24 h. (C and D) Flow cytometric analysis of early and late apoptosis and western blot analysis of the expression levels of BAX and BCL2 in T24 and 5637 cells. KNSTRN-silenced and si-NC cells were treated with either DMSO or rapamycin (Rapa, T24, 20 nM; 5637, 50 nM) for 24 h. Significance is determined by one-way ANOVA. Data are presented as the mean ± SD ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: Rapamycin (Rapa) , Selleck , Cat# S1039.

Techniques: Knockdown, In Vitro, CCK-8 Assay, Western Blot, Expressing

Journal: iScience

Article Title: KNSTRN knockdown impairs autophagy flux to inhibit bladder cancer progression

doi: 10.1016/j.isci.2026.114734

Figure Lengend Snippet: Knockdown of KNSTRN inhibits tumor growth and suppresses autophagic flux in vivo (A and B) Three groups of tumors, sh-NC, sh-KNSTRN, sh-KNSTRN+Rapa, were harvested and photographed ( n = 5). (C) Tumor growth curves in xenograft mice treated with sh-NC, sh-KNSTRN, and sh-KNSTRN+Rapa. (D) Immunohistochemical staining of KNSTRN, LC3, and p62 in xenograft tumor tissues treated with sh-NC, sh-KNSTRN, and sh-KNSTRN+Rapa. Significance is determined by one-way ANOVA. Data are presented as the mean ± SD ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns, not significantly different.

Article Snippet: Rapamycin (Rapa) , Selleck , Cat# S1039.

Techniques: Knockdown, In Vivo, Immunohistochemical staining, Staining