reca 1  (Proteintech)

Journal: Bioactive Materials

Article Title: Targeting VEGFR2 inhibition within a spatially-confined conduit promotes nerve self-resolution and alleviates mechanical allodynia

doi: 10.1016/j.bioactmat.2026.03.009

Figure Lengend Snippet: Prevention of neuroma formation by a spatially confined conduit filled with GelMA MAVP MPS. ( A ) Illustration of 3D-printed GelMA MPs loaded with MAVP and the proposed mechanism of action within the neural conduit. ( B ) Representative images and ( C ) quantitative scores of autotomy behavior over 12 weeks (n = 6). ( D ) Representative gait footprints at 12 weeks post-surgery. ( E ) Quantification of left hindlimb stance duration (n = 6) and ( F ) maximum contact area (n = 6). ( G ) IF staining of p-VEGFR2 activation and ( H ) IF staining of neovascularization marker RECA-1. (I) Quantification of p-VEGFR2-positive area percentage (n = 6). and ( J ) quantification of RECA-1-positive area percentage (n = 6). ( K ) Regenerated nerve length measurements (n = 6). Mean values are shown and error bars represent ± s.d., as analyzed by one-way ANOVA followed by the Tukey-Kramer test in ( C , E , F , I , J and K ). Biological replicates were used for all experiments. ns, p > 0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: The following primary antibodies were used for the subsequent steps: anti-Yap (mouse, 1:200, Santa sc-376830); anti-p-VEGFR2 (rabbit, 1:100 Invitrogen, PA5-105765); α-SMA (rabbit, 1:200, Proteintech 14395-1-AP); Reca-1 (mouse, 1:200, Santa sc-52665); anti-CD31 (mouse, 1:200, Santa sc-13537); anti-Ki67 (rabbit, 1:150, Cell Signaling 9129S); anti-NF-200 (mouse, 1:200, Sigma, SAB4200747); anti-MBP (rabbit, 1:200, Abcam ab218011); anti-F4/80 (mouse, 1:200, Santa sc-377009); Iba-1 (rabbit, 1:150, Abcam ab178846); anti-CGRP (rabbit, 1:400, Abcam ab283568); anti-TRPA1 (mouse, 1:200, Santa sc-376495); anti-CD86 (rabbit, 1:200, Proteintech 30691-1-AP); CD206 (rabbit, 1:200, Proteintech 18704-1-AP).

Techniques: Staining, Activation Assay, Marker

Journal: bioRxiv

Article Title: Homologous recombination delayed repair in oocytes in the bdelloid rotifer Adineta vaga post radiation

doi: 10.64898/2026.04.30.722046

Figure Lengend Snippet: Boxplots of the expression levels (Transcript Per Millions: TPMs) of genes involved in HR: Rad51_1, Rad51_2, and Rad54 showing low expression levels (TPMs<50) in all conditions. Rad51_1 and Rad54 do not show any over-expression post X-rays radiation (500 Gy) whereas Rad51_2 shows a little over-expression 2.5 and 8 hours post radiation. Transcriptomic data from .

Article Snippet: Strand cDNA was synthesized from isolated total RNA (1 μg, DNase-treated) of A. vaga for the genes 60S ribosomal protein, Actin, or from irradiated A. vaga 2.5h post 500 Gy X- ray radiation (when the expression was observed to be at its highest level: ( ) for the genes Ligase E, Heat Shock Protein HSP82, KU80, Rad51_1, Rad51_2, Rad54, PARP_1, PARP_2 using Ambion RNAqueous®-4PCR Kit ( Fisher Scientific, Aalst, Belgium).

Techniques: Expressing, Over Expression

Journal: bioRxiv

Article Title: Homologous recombination delayed repair in oocytes in the bdelloid rotifer Adineta vaga post radiation

doi: 10.64898/2026.04.30.722046

Figure Lengend Snippet: Spatial expression of genes involved in the HR repair pathway (Rad51_1, Rad51_2, Rad54) and PCNA in cryosections of the bdelloid rotifer Adineta vaga . The first row represents the DAPI staining, the second row the probe staining through ISH. The dotted violet line surrounds nurse nuclei, while the continuous red line surrounds a maturing oocyte, the green dotted line surrounds the entire section of a rotifer individual, the blue line surrounds the laid egg. The first column represents the hydrated non-irradiated rotifers (control), the second column hydrated rotifers 2.5h post X-rays radiation (500 Gy), the third column a hydrated rotifer 5-days post-irradiation with a developing oocyte, and in the fourth column a developing egg laid by a mother, 5 days post-irradiation.

Article Snippet: Strand cDNA was synthesized from isolated total RNA (1 μg, DNase-treated) of A. vaga for the genes 60S ribosomal protein, Actin, or from irradiated A. vaga 2.5h post 500 Gy X- ray radiation (when the expression was observed to be at its highest level: ( ) for the genes Ligase E, Heat Shock Protein HSP82, KU80, Rad51_1, Rad51_2, Rad54, PARP_1, PARP_2 using Ambion RNAqueous®-4PCR Kit ( Fisher Scientific, Aalst, Belgium).

Techniques: Expressing, Staining, Irradiation, Control