anti rab7  (Proteintech)

Journal: bioRxiv

Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells

doi: 10.64898/2026.02.12.705585

Figure Lengend Snippet: (A-B) Wing discs endogenously expressing either YFP-Rab4 (A) or YFP-Rab7 (B) and stained for Wg. One example of a colocalising WgLV is shown for each Rab. (C) Average radial fluorescence profiles of YFP-Rab4-positive (N= 13 wing discs) or YFP-Rab7-positive (N = 4 wing discs) WgLVs. Error bars indicate SD. Magenta indicates the Wg profile, and green the corresponding Rab. In grey and light grey we show the profiles of bright Wg-containing particles that did not pass our pipeline to identify WgLVs and hence removed from the WgLV plots (“Rmvd”). (D) Examples of WgLVs ( magenta/grayscale ) positive for YFP-Rab4 ( green ), anti-Rab7 ( blue ) and TagRFP-Vps35 ( yellow ). (E) Diagram illustrating the different populations of WgLVs in terms of Rab4/7 colocalization.

Article Snippet: The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).

Techniques: Expressing, Staining, Fluorescence

Journal: Life Science Alliance

Article Title: Elevated Piezo levels cause structural and functional alterations in Drosophila garland nephrocytes

doi: 10.26508/lsa.202503515

Figure Lengend Snippet: (A) Assessment of cortical Cubilin and Cubilin 2 expression reveals a significant increase of Cubilin in garland nephrocytes expressing Piezo wild type. t test: * P < 0.05. Control: w; sns -Gal4/+;UAS- dicer2 /+. Piezo WT: w; sns -Gal4/+;UAS- dicer2 /UAS- Piezo -FLAG. (B) Loss of Piezo does not result in alterations of cortical Cubilin and Cubilin 2. Wildtype: w 1118 ; Piezo KO : w;+; Piezo ko . (C) AgNO 3 toxin assays showing no delay in pupation caused by GFP expression in nephrocytes. Control: w; sns -Gal4/+;UAS- dicer2 /+. UAS-GFP: w; sns -Gal4/+;UAS- dicer2 /UAS-GFP. Depicted are means ± SEM. Each timepoint represents three independent n’s with 15–20 first instar larvae each. (D) Rab7 antibody has been used to visualize late endosomes. Neither overexpression (28°C) nor depletion of Piezo resulted in significant changes of Rab7. Scale bar = 25 and 5 μm (magnification). t test. (E) FITC-Albumin uptake assays reveal no dilution effect of expressing two constructs under UAS control (Piezo WT + GFP). FITC-Albumin accumulation has been visualized by anti-albumin antibody–based staining coupled to a Cy3 secondary antibody, to exclude an overlap of the FITC signal with the GFP signal. Scale bar = 25 μm. One-way ANOVA with Tukey’s multiple comparisons test: *** P < 0.001. Control: w; sns -Gal4/+;UAS- dicer2 /+; UAS-GFP: w; sns -Gal4/+;UAS- dicer2 /UAS-GFP; Piezo WT: w; sns -Gal4/+; UAS- Piezo -GFP/+; Piezo WT + GFP: w; sns -Gal4/+; UAS-GFPUAS- Piezo -GFP.

Article Snippet: Rab7 , Developmental Studies Hybridoma Bank , Rab7 , Mouse , 1:25.

Techniques: Expressing, Control, Over Expression, Construct, Staining

Journal: bioRxiv

Article Title: Smoothened turnover regulated by Hedgehog signaling in Drosophila

doi: 10.64898/2026.01.08.698469

Figure Lengend Snippet: (A) Schematic of frontal depiction of the wing pouch of the wing disc with the A (gray) and P (green) compartments indicated by gray and green, respectively; boxes indicate positions of (F-K) images. (B) White dotted lines delineate wing pouch and compartment border of disc expressing BAC encoded Hh:GFP. (C) Sagittal section of the wing disc expressing BAC encoded Hh:GFP (α-GFP antibody staining). Septate junction stained with α-DLG (red) marks septate junction; phalloidin marks adherens junction (blue). (D) Graph of Hh:GFP fluorescence in A and P compartments. (E) Schematic of sagittal wing pouch section with apical and basolateral compartments, Hh:GFP (green) and Rab7 (red) indicated. (F-G”) Frontal views of region of the A compartment of wing discs with BAC-encoded Hh:GFP detected with α-GFP antibody (green) and stained with α-Rab7 antibody (red) and Phalloidin (blue). (H,H’) Sagittal section showing total Hh signal (H) and Hh not colocalized with Rab7. White and yellow arrows indicate peripodial membrane and apical compartment, respectively. (I-K’) Same as (F-H’) for P compartment. (K) Graph showing the proportion of BAC encoded Hh:GFP signal that overlaps with Rab7 in apical/basolateral optical sections.

Article Snippet: The following antibodies were used: mouse α-GFP (Roche), rabbit α-RFP (Rockland), mouse α-Ptc (DSHB, Apa1), mouse α-En (DSHB, 4D9), DAPI, Phalloidin, mouse α-Dlg1, (DSHB, 4F3), α-Rab7 , α-Smo (DSHB, 20C6), Alexa633 conjugated-Phalloidin (Invitrogen), DAPI (Invitrogen), AbN ( ).

Techniques: Expressing, Staining, Fluorescence, Membrane

Journal: bioRxiv

Article Title: Smoothened turnover regulated by Hedgehog signaling in Drosophila

doi: 10.64898/2026.01.08.698469

Figure Lengend Snippet: (A-E) Frontal views of unfixed wing discs, optical section close to the apical surface; left, anterior; right, posterior. (A,B) Wing discs expressing BAC-encoded GFP:Cherry:Smo without (A) and with ammonium chloride (B); GFP channel (green), RFP channel (red). (C) Wing disc expressing BAC-encoded GFP:Smo (green) and Rbcn3A RNAi (expressed during L3 at 29°C) in the dorsal region (below white dashed line) using ( ap-Gal4/tub-Gal80 ts ; GFP:Smo/Rbcn3A-RNAi ). (D,E) Unfixed wing discs expressing Cherry:Smo (red) and Rab5:YFP (green) (D) or Cherry:Smo (red) and Rab7:YFP (green). (F) Amount of Cherry:Smo/Rab7 double positive and Cherry:Smo /Rab5 double positive puncta.

Article Snippet: The following antibodies were used: mouse α-GFP (Roche), rabbit α-RFP (Rockland), mouse α-Ptc (DSHB, Apa1), mouse α-En (DSHB, 4D9), DAPI, Phalloidin, mouse α-Dlg1, (DSHB, 4F3), α-Rab7 , α-Smo (DSHB, 20C6), Alexa633 conjugated-Phalloidin (Invitrogen), DAPI (Invitrogen), AbN ( ).

Techniques: Expressing

Journal: Journal of Virology

Article Title: A recurrent adaptive mutation in the transmembrane 2B protein of an insect picorna-like virus in a nonnative host

doi: 10.1128/jvi.01239-25

Figure Lengend Snippet: Subcellular localization of the WT and D29N CrPV 2B proteins. Confocal microscopy images of Drosophila S2 cells transfected with plasmids encoding the indicated proteins. Cells were fixed 48 h after transfection and stained with ( A ) antibodies against the ER protein Calnexin 99A (Cnx99a) and the cis Golgi protein GM130, and ( B ) antibodies against the lysosome protein Rab7 (magenta). Nuclei were stained with DAPI. In the merged images in ( A ), eGFP is shown in green, Cnx99a in red, GM130 in magenta, and DAPI in blue.

Article Snippet: Calnexin 99A antibody (Developmental Studies Hybridoma Bank, Cnx99A 6-2-1; RRID:AB_2722011) was diluted 1:10, GM130 antibody (Abcam, ab30637) was diluted 1:250, Rab7 antibody (Developmental Studies Hybridoma Bank, RRID:AB_2722471) was diluted 1:10, and V5 antibody (Invitrogen, 46-0705) was diluted 1:200.

Techniques: Confocal Microscopy, Transfection, Staining