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rab11fip5 (OriGene)

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OriGene rab11fip5

FIGURE 1 Extracellular vesicles derived from brain-seeking MDA-MB-231 cells (Br-EVs) modulate the expression of multiple Rab11 effector proteins (rab11fips) involved in intracytoplasmic vesicle trafficking and recycling. (a) Schematic representing degradation and recycling pathways in brain endothelial cells (BECs). Created using Biorender. (b) Schematic depicting the most widely acknowledged mechanisms of interaction between Rab11fip2, Rab11fip3, <t>Rab11fip5,</t> and the respective motor proteins for the tethering and transport of rab11 vesicles on cytoskeleton filaments. Created using Biorender. (c–e) Western blot quantification, representative immunofluorescent image, and fluorescence intensity quantification of Rab11fip2, (f–h) Rab11fip3, and (j–k) Rab11fip5 protein expression in BECs following treatment with PBS, P-EVs, and Br-EVs (mean ± SD; technical triplicates in 3

Rab11fip5, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rab11fip5/RAB11FIP5+(NM_015470)+Human+Tagged+ORF+Clone/pm39868462-69-25-31
Average 93 stars, based on 1 article reviews

rab11fip5 - by Bioz Stars, 2026-09

93/100 stars

Images

1) Product Images from "Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells."

Article Title: Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells.

Journal: Journal of extracellular vesicles

doi: 10.1002/jev2.70038

Figure Legend Snippet: FIGURE 1 Extracellular vesicles derived from brain-seeking MDA-MB-231 cells (Br-EVs) modulate the expression of multiple Rab11 effector proteins (rab11fips) involved in intracytoplasmic vesicle trafficking and recycling. (a) Schematic representing degradation and recycling pathways in brain endothelial cells (BECs). Created using Biorender. (b) Schematic depicting the most widely acknowledged mechanisms of interaction between Rab11fip2, Rab11fip3, Rab11fip5, and the respective motor proteins for the tethering and transport of rab11 vesicles on cytoskeleton filaments. Created using Biorender. (c–e) Western blot quantification, representative immunofluorescent image, and fluorescence intensity quantification of Rab11fip2, (f–h) Rab11fip3, and (j–k) Rab11fip5 protein expression in BECs following treatment with PBS, P-EVs, and Br-EVs (mean ± SD; technical triplicates in 3

Techniques Used: Derivative Assay, Expressing, Western Blot, Fluorescence

FIGURE 2 Cerebral microvessels isolated from the brain cortex of mice treated with Br-EVs show decreased Rab11fip2 and increased Rab11fip3 and Rab11fip5 levels in response to Br-EV treatment. (a) Western blot quantification of Rab11fip2, Rab11fip3, and Rab11fip5 in cerebral microvessel lysates of mice treated with PBS, P-EVs, and Br-EVs. PBS, P-EV and Br-EV groups were run on separate gels under the same conditions and normalization to GAPDH was performed individually for each sample with raw values being presented. Uncropped gels are provided in the Figure S5 (mean ± SD; n = 4 for PBS and Br-EV groups and n = 6 and 5 for the P-EV group). (b) Representative fluorescent images of cerebral microvessels from the brains of mice treated with PBS, P-EVs, and Br-EVs and stained with Rab11fip2, Rab11fip3, and Rab11fip5 (green) and nuclei (blue). Scale bars 50 µm. (c) Quantification of the signal mean fluorescence intensity (MFI) of the cerebral microvessels of mice treated with PBS, P-EVs, and Br-EVs and stained for Rab11fip2, Rab11fip3, and Rab11fip5 (normalized to the image background; mean ± SD; n = 5 for the PBS group, n = 6 for the P-EV group, and n = 4 for the Br-EV group). Statistical analysis was performed using the Mann–Whitney test (a) and one-way ANOVA with Tukey’s multiple comparison test (c) (Wu and Voeltz 2021; Lee et al. 2019; Mohrmann et al. 2002; Busatto et al. 2020). ns = not significant; *p ≤0.0332; **p ≤0.0021; ****p ≤0.0001.

Figure Legend Snippet: FIGURE 2 Cerebral microvessels isolated from the brain cortex of mice treated with Br-EVs show decreased Rab11fip2 and increased Rab11fip3 and Rab11fip5 levels in response to Br-EV treatment. (a) Western blot quantification of Rab11fip2, Rab11fip3, and Rab11fip5 in cerebral microvessel lysates of mice treated with PBS, P-EVs, and Br-EVs. PBS, P-EV and Br-EV groups were run on separate gels under the same conditions and normalization to GAPDH was performed individually for each sample with raw values being presented. Uncropped gels are provided in the Figure S5 (mean ± SD; n = 4 for PBS and Br-EV groups and n = 6 and 5 for the P-EV group). (b) Representative fluorescent images of cerebral microvessels from the brains of mice treated with PBS, P-EVs, and Br-EVs and stained with Rab11fip2, Rab11fip3, and Rab11fip5 (green) and nuclei (blue). Scale bars 50 µm. (c) Quantification of the signal mean fluorescence intensity (MFI) of the cerebral microvessels of mice treated with PBS, P-EVs, and Br-EVs and stained for Rab11fip2, Rab11fip3, and Rab11fip5 (normalized to the image background; mean ± SD; n = 5 for the PBS group, n = 6 for the P-EV group, and n = 4 for the Br-EV group). Statistical analysis was performed using the Mann–Whitney test (a) and one-way ANOVA with Tukey’s multiple comparison test (c) (Wu and Voeltz 2021; Lee et al. 2019; Mohrmann et al. 2002; Busatto et al. 2020). ns = not significant; *p ≤0.0332; **p ≤0.0021; ****p ≤0.0001.

Techniques Used: Isolation, Western Blot, Staining, Fluorescence, MANN-WHITNEY, Comparison

FIGURE 5 BECs knocked down (KD) for Rab7 and overexpressing (OE) Rab11fip5 display motility and morphology patterns similar to those of BECs treated with Br-EVs. UMAP representation of BEC populations KD for Rab7 and Rab11fip2 and OE for Rab11fip3 and Rab11fip5 (from left to right), analysed for morphology features using (a) confocal microscopy (to be compared with Figure 3c) and (c) live cell imaging (to be compared with Figure 3j), and for motility features using (e) QPI (to be compared with Figure 4c) and (g) live cell imaging (to be compared with Figure 4j). (b, d, f and h) Tables showing variations in cluster distribution and the percentage of each cluster within each respective sample.

Figure Legend Snippet: FIGURE 5 BECs knocked down (KD) for Rab7 and overexpressing (OE) Rab11fip5 display motility and morphology patterns similar to those of BECs treated with Br-EVs. UMAP representation of BEC populations KD for Rab7 and Rab11fip2 and OE for Rab11fip3 and Rab11fip5 (from left to right), analysed for morphology features using (a) confocal microscopy (to be compared with Figure 3c) and (c) live cell imaging (to be compared with Figure 3j), and for motility features using (e) QPI (to be compared with Figure 4c) and (g) live cell imaging (to be compared with Figure 4j). (b, d, f and h) Tables showing variations in cluster distribution and the percentage of each cluster within each respective sample.

Techniques Used: Confocal Microscopy, Live Cell Imaging

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Journal: Journal of extracellular vesicles

Article Title: Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells.

doi: 10.1002/jev2.70038

Figure Lengend Snippet: FIGURE 1 Extracellular vesicles derived from brain-seeking MDA-MB-231 cells (Br-EVs) modulate the expression of multiple Rab11 effector proteins (rab11fips) involved in intracytoplasmic vesicle trafficking and recycling. (a) Schematic representing degradation and recycling pathways in brain endothelial cells (BECs). Created using Biorender. (b) Schematic depicting the most widely acknowledged mechanisms of interaction between Rab11fip2, Rab11fip3, Rab11fip5, and the respective motor proteins for the tethering and transport of rab11 vesicles on cytoskeleton filaments. Created using Biorender. (c–e) Western blot quantification, representative immunofluorescent image, and fluorescence intensity quantification of Rab11fip2, (f–h) Rab11fip3, and (j–k) Rab11fip5 protein expression in BECs following treatment with PBS, P-EVs, and Br-EVs (mean ± SD; technical triplicates in 3

Article Snippet: The cell pellet was resuspended in 100 μL of buffer, mixed with 2 μg of Rab11fip3 (human tagged orf clone rc227246, Origene Technologies Inc.) and Rab11fip5 (human tagged orf clone rc206173, Origene Technologies Inc.) plasmids, and electroporated using the S-05 Program of the Amaxa Biosystem Nucleofector.

Techniques: Derivative Assay, Expressing, Western Blot, Fluorescence

Journal: Journal of extracellular vesicles

Article Title: Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells.

doi: 10.1002/jev2.70038

Figure Lengend Snippet: FIGURE 2 Cerebral microvessels isolated from the brain cortex of mice treated with Br-EVs show decreased Rab11fip2 and increased Rab11fip3 and Rab11fip5 levels in response to Br-EV treatment. (a) Western blot quantification of Rab11fip2, Rab11fip3, and Rab11fip5 in cerebral microvessel lysates of mice treated with PBS, P-EVs, and Br-EVs. PBS, P-EV and Br-EV groups were run on separate gels under the same conditions and normalization to GAPDH was performed individually for each sample with raw values being presented. Uncropped gels are provided in the Figure S5 (mean ± SD; n = 4 for PBS and Br-EV groups and n = 6 and 5 for the P-EV group). (b) Representative fluorescent images of cerebral microvessels from the brains of mice treated with PBS, P-EVs, and Br-EVs and stained with Rab11fip2, Rab11fip3, and Rab11fip5 (green) and nuclei (blue). Scale bars 50 µm. (c) Quantification of the signal mean fluorescence intensity (MFI) of the cerebral microvessels of mice treated with PBS, P-EVs, and Br-EVs and stained for Rab11fip2, Rab11fip3, and Rab11fip5 (normalized to the image background; mean ± SD; n = 5 for the PBS group, n = 6 for the P-EV group, and n = 4 for the Br-EV group). Statistical analysis was performed using the Mann–Whitney test (a) and one-way ANOVA with Tukey’s multiple comparison test (c) (Wu and Voeltz 2021; Lee et al. 2019; Mohrmann et al. 2002; Busatto et al. 2020). ns = not significant; *p ≤0.0332; **p ≤0.0021; ****p ≤0.0001.

Techniques: Isolation, Western Blot, Staining, Fluorescence, MANN-WHITNEY, Comparison

Journal: Journal of extracellular vesicles

Article Title: Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells.

doi: 10.1002/jev2.70038

Figure Lengend Snippet: FIGURE 5 BECs knocked down (KD) for Rab7 and overexpressing (OE) Rab11fip5 display motility and morphology patterns similar to those of BECs treated with Br-EVs. UMAP representation of BEC populations KD for Rab7 and Rab11fip2 and OE for Rab11fip3 and Rab11fip5 (from left to right), analysed for morphology features using (a) confocal microscopy (to be compared with Figure 3c) and (c) live cell imaging (to be compared with Figure 3j), and for motility features using (e) QPI (to be compared with Figure 4c) and (g) live cell imaging (to be compared with Figure 4j). (b, d, f and h) Tables showing variations in cluster distribution and the percentage of each cluster within each respective sample.

Techniques: Confocal Microscopy, Live Cell Imaging

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Structured Review

Images

1) Product Images from "Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells."

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