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puc118 hinc ii bap  (TaKaRa)


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    Structured Review

    TaKaRa puc118 hinc ii bap
    Puc118 Hinc Ii Bap, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc118/Hinc+II/pmc13050634-200-13-17
    Average 95 stars, based on 437 article reviews
    puc118 hinc ii bap - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Mutant type 2-deoxy-scyllo-inosose synthase
    Article Snippet: .. In the case of using, for example, Escherichia coli as a host cell, examples of the plasmid include pBTrp2, pBTac1, and pBTac2 (manufactured by Boehringer Mannheim), pKK233-2 (manufactured by Pharmacia), pSE280 (manufactured by Invitrogen), pGEMEX-1 (manufactured by Promega), pQE-8 and pQE-30 (manufactured by QIAGEN), pBluescript II SK (+) and pBluescript II SK (−) (manufactured by Stratagene), pET-3 (manufactured by Novagen), pUC18, pSTV28, pSTV29, and pUC118 (manufactured by Takara Shuzo Co., Ltd.), pLEX (manufactured by Invitrogen), pQE80L (manufactured by QIAGEN), and pBR322. .. In the case of using, for example, Escherichia coli as a host cell, examples of the plasmid include pBTrp2, pBTac1, and pBTac2 (manufactured by Boehringer Mannheim), pKK233-2 (manufactured by Pharmacia), pSE280 (manufactured by Invitrogen), pGEMEX-1 (manufactured by Promega), pQE-8 and pQE-30 (manufactured by QIAGEN), pBluescript II SK (+) and pBluescript II SK (−) (manufactured by Stratagene), pET-3 (manufactured by Novagen), pUC18, pSTV28, pSTV29, and pUC118 (manufactured by Takara Shuzo Co., Ltd.), pLEX (manufactured by Invitrogen), pQE80L (manufactured by QIAGEN), and pBR322.

    Article Title: Muconic acid-producing transformed microorganism and use thereof
    Article Snippet: .. 212,p77-86,1998; the entire description is incorporated by reference herein), pPS858 (see Gene,vol.212,p77-86,1998; the entire description is incorporated by reference herein), pUC118 (Takara Bio), pJB866 (see Plasmid,vol.38,p35-51,1997; the entire description is incorporated by reference herein), pMCL200 (see Gene,vol.162,p157-158,1995; the entire description is incorporated by reference herein), pQE30 (Qiagen), pUC119 (Takara Bio), pUC18 (Takara Bio), and pBR322 (Takara Bio); known or commercially available bacteriophage vectors, such as AEMBL3 (Stratagene), and pAK405 (Andreas Kaczmarczyk et al., Applied and Environmental Microbiology, 2012, 78 (10) 3774-3777; the entire description is incorporated by reference herein). .. If a large number of the constructed recombinant DNA is required, for example, Escherichia coli, preferably Escherichia coli JM109 strain (Takara Bio) or Escherichia coli DH5a strain (Takara Bio) may be transformed with the recombinant DNA.

    Article Title: Method of increasing lipid productivity in nannochloropsis by introducing a gene encoding both a thioredoxin domain and a thioredoxin reductase domain
    Article Snippet: Furthermore, the vector may also be a vector such as a plasmid capable of self-proliferation and self-replication outside the chromosome, or may also be a vector which is incorporated into the chromosome. .. Specific examples of the vector that can be used preferably in the present invention include pUC18 (manufactured by Takara Bio), pUC19 (manufactured by Takara Bio), pUC118 (manufactured by Takara Bio), P66 (Chlamydomonas Center), P-322 (Chlamydomonas Center), pPha-T1 (see Journal of Basic Microbiology, 2011, vol. .. 51, p. 666-672) and pJET1 (manufactured by COSMO BIO).

    Amplification:

    Article Title: Modified promoter
    Article Snippet: .. Using the genomes of the PC-3-7 strain as a template, primer xyn3reps (SEQ ID NO:25) and primer xyn3repa (SEQ ID NO:26), a 748 bp of downstream region of the xyn3 was amplified and introduced into HincII site of the pUC118 (Takara) (pUxyn3DR). ..

    Purification:

    Article Title: Genetic resistance in barley against Japanese soil-borne wheat mosaic virus functions in the roots
    Article Snippet: The resulting amplicon (expected size: 338 bp) was verified by separation through a 1.5% agarose gel and purified using a QIA quick PCR Purification Kit (Qiagen) following the manufacturer’s protocol. .. The purified amplicons were ligated into pUC118 (Takara Bio inc.) and transformed into E. coli DH5α competent cells (Invitrogen) for sequencing using a Hitachi 3130X Genetic Analyzer. .. The RNAs required to conduct a TaqMan assay of JSBWMV were extracted from 100 mg fresh plant material using a Qiagen RNeasy Plant Mini Kit fitted with a column DNase Digestion Kit (Takara Bio inc.) using the suppliers’ protocols.

    Transformation Assay:

    Article Title: Genetic resistance in barley against Japanese soil-borne wheat mosaic virus functions in the roots
    Article Snippet: The resulting amplicon (expected size: 338 bp) was verified by separation through a 1.5% agarose gel and purified using a QIA quick PCR Purification Kit (Qiagen) following the manufacturer’s protocol. .. The purified amplicons were ligated into pUC118 (Takara Bio inc.) and transformed into E. coli DH5α competent cells (Invitrogen) for sequencing using a Hitachi 3130X Genetic Analyzer. .. The RNAs required to conduct a TaqMan assay of JSBWMV were extracted from 100 mg fresh plant material using a Qiagen RNeasy Plant Mini Kit fitted with a column DNase Digestion Kit (Takara Bio inc.) using the suppliers’ protocols.

    Sequencing:

    Article Title: Genetic resistance in barley against Japanese soil-borne wheat mosaic virus functions in the roots
    Article Snippet: The resulting amplicon (expected size: 338 bp) was verified by separation through a 1.5% agarose gel and purified using a QIA quick PCR Purification Kit (Qiagen) following the manufacturer’s protocol. .. The purified amplicons were ligated into pUC118 (Takara Bio inc.) and transformed into E. coli DH5α competent cells (Invitrogen) for sequencing using a Hitachi 3130X Genetic Analyzer. .. The RNAs required to conduct a TaqMan assay of JSBWMV were extracted from 100 mg fresh plant material using a Qiagen RNeasy Plant Mini Kit fitted with a column DNase Digestion Kit (Takara Bio inc.) using the suppliers’ protocols.

    Clone Assay:

    Article Title: A novel high-throughput approach for transforming filamentous fungi employing a droplet-based microfluidic platform.
    Article Snippet: .. The DNA fragment was cloned into the EcoRV site of pUC118 (Takara Bio, Shiga, Japan) using the In-Fusion Cloning Kit (Takara Bio, Shiga, Japan). ..

    Cloning:

    Article Title: A novel high-throughput approach for transforming filamentous fungi employing a droplet-based microfluidic platform.
    Article Snippet: .. The DNA fragment was cloned into the EcoRV site of pUC118 (Takara Bio, Shiga, Japan) using the In-Fusion Cloning Kit (Takara Bio, Shiga, Japan). ..



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