Saline:Article Title: CUDC-907 inhibits osteosarcoma through upregulation of PTX3 and inhibition of PI3K-AKT signaling.
Article Snippet: Osteosarcoma is the most common primary malignant bone tumor in adolescents, causing severe socioeconomic burden on society.. CUDC-907 is a newly developed drug by directly inhibiting PI3K and exerts effects on many tumors.. It has also been reported that CUDC-907 inhibits the stemness of neuroblastoma by targeting on pentraxin 3 (PTX3), which, as an innate immune molecule, is widely involved in a wide range of physiological and pathological activities of the organism and in the development of a variety of tumors, but its role in osteosarcoma has not been investigated.
Incubation:Article Title: CUDC-907 inhibits osteosarcoma through upregulation of PTX3 and inhibition of PI3K-AKT signaling.
Article Snippet: Osteosarcoma is the most common primary malignant bone tumor in adolescents, causing severe socioeconomic burden on society.. CUDC-907 is a newly developed drug by directly inhibiting PI3K and exerts effects on many tumors.. It has also been reported that CUDC-907 inhibits the stemness of neuroblastoma by targeting on pentraxin 3 (PTX3), which, as an innate immune molecule, is widely involved in a wide range of physiological and pathological activities of the organism and in the development of a variety of tumors, but its role in osteosarcoma has not been investigated.
Article Title: Identification of Potential Therapeutic Targets for Sepsis Using Mendelian Randomization and Integrated eQTL/pQTL Analysis
Article Snippet: Equal protein amounts (approximately 20–40 μg per sample) were separated using SDS-PAGE gels and electrophoretically transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. Membranes underwent blocking with 5% skimmed milk in TBST for one hour at ambient temperature and were subsequently incubated overnight (4°C) with primary antibodies specific to BCL6 (ab33901), PTX3 (ab90806), IL7R (ab314106), BTN3A2 (Cat No. 15148-1-AP, Proteintech), LGALS1 (ab150427), and the internal control GAPDH (60004-1-Ig, Proteintech). .. Tissue samples and cells were lysed using RIPA buffer containing protease inhibitors, and protein concentrations were measured via a BCA assay kit (Beyotime, China).
Article Title: Identification of Potential Therapeutic Targets for Sepsis Using Mendelian Randomization and Integrated eQTL/pQTL Analysis
Article Snippet: Equal protein amounts (approximately 20–40 μg per sample) were separated using SDS-PAGE gels and electrophoretically transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. Membranes underwent blocking with 5% skimmed milk in TBST for one hour at ambient temperature and were subsequently incubated overnight (4°C) with primary antibodies specific to BCL6 (ab33901), PTX3 (ab90806), IL7R (ab314106), BTN3A2 (Cat No. 15148-1-AP, Proteintech), LGALS1 (ab150427), and the internal control GAPDH (60004-1-Ig, Proteintech). .. Following washing steps, the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Proteintech) at room temperature for one hour.
Article Title: TAF15 promotes the healing of diabetic foot ulcers by mediating the transcriptional activation of APOE through CEBPB to regulate PTX3.
Article Snippet: Over the past 40 years, the number of patients with diabetes has increased dramatically worldwide.. Diabetic foot ulcers (DFU) are among the most feared complications, ultimately necessitating more than one million lower-limb amputations each year (McDermott et al. 2023).. Current treatment approaches, such as managing blood glucose levels, protecting nerves, and enhancing circulation, do not significantly aid in ulcer healing, exposing patients to life-threatening infections or soaring healthcare costs (Jeffcoate et al. 2024).
Article Title: A study on the establishment of a traumatic brain injury model of tree shrews and the observation of neuroinflammatory responses
Article Snippet: Total protein was separated by 10 % SDS-PAGE and transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore, Billerica, USA). .. Membranes were blocked with 5 % BSA at room temperature for 2 h and incubated overnight at 4°C with primary antibodies: Serping1 (66882–1-Ig, Proteintech, 1:1000), Ptx3 (13797–1-AP, Proteintech, 1:1000), and rabbit anti-β-actin (20536–1-AP, Proteintech, 1:5000). ..
Blocking Assay:Article Title: Identification of Potential Therapeutic Targets for Sepsis Using Mendelian Randomization and Integrated eQTL/pQTL Analysis
Article Snippet: Equal protein amounts (approximately 20–40 μg per sample) were separated using SDS-PAGE gels and electrophoretically transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. Membranes underwent blocking with 5% skimmed milk in TBST for one hour at ambient temperature and were subsequently incubated overnight (4°C) with primary antibodies specific to BCL6 (ab33901), PTX3 (ab90806), IL7R (ab314106), BTN3A2 (Cat No. 15148-1-AP, Proteintech), LGALS1 (ab150427), and the internal control GAPDH (60004-1-Ig, Proteintech). .. Tissue samples and cells were lysed using RIPA buffer containing protease inhibitors, and protein concentrations were measured via a BCA assay kit (Beyotime, China).
Article Title: Pentraxin 3 ameliorates glucocorticoid-induced osteonecrosis of the femoral head via TLR4/NF-κB/FGF21 signaling axis
Article Snippet: .. The primary antibodies including beta-Actin (1:1000 dilution, M20005F, Abmart), PTX3 (1:1000, 13797-1-AP, Proteintech), Bax (1:1000, T40051 , Abmart), Bcl-2 (1:500, sc-509, Santa Cruz Biotechnology), Osteocalcin (1:1000 dilution, DF12303, Affinity), ALP (1:1000 dilution, DF6225, Affinity), Collagen I (1:1000 dilution, A24112, ABclonal), Runx2 (1:1000 dilution, AF5186, Affinity), OPN (1:200 dilution, AF0227, Affinity), phosphorylated p65 (p-p65,1:1000 dilution, AF2006, Affinity), phosphorylated IκB alpha (p-IκB, 1:1000 dilution, 82349-1-RR, Proteintech), Toll like receptor 4 (TLR4, 1:1000 dilution, AF7017, Affinity), Mutant myeloid differentiation primary response 88 (MyD88, 1:1000 dilution, AF5195, Affinity), p65 (1:1000 dilution, AF5006, Affinity), IκB alpha (1:1000 dilution, AF5002, Affinity) and FGF21 (1:1000 dilution, TC52978S, Abmart) diluted in the blocking buffer, were applied to the membrane and allowed to incubate overnight at 4 °C to detect the target proteins. .. Following incubation with the appropriate anti-rabbit or anti-mouse secondary antibodies for 1 h at 37 °C, the protein bands were visualized using the Odyssey infrared imaging system (LI-COR® Biosciences, USA) and analyzed according to the Odyssey software manual instructions. β-actin served as the internal control.
Article Title: Identification of Potential Therapeutic Targets for Sepsis Using Mendelian Randomization and Integrated eQTL/pQTL Analysis
Article Snippet: Equal protein amounts (approximately 20–40 μg per sample) were separated using SDS-PAGE gels and electrophoretically transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. Membranes underwent blocking with 5% skimmed milk in TBST for one hour at ambient temperature and were subsequently incubated overnight (4°C) with primary antibodies specific to BCL6 (ab33901), PTX3 (ab90806), IL7R (ab314106), BTN3A2 (Cat No. 15148-1-AP, Proteintech), LGALS1 (ab150427), and the internal control GAPDH (60004-1-Ig, Proteintech). .. Following washing steps, the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Proteintech) at room temperature for one hour.
Article Title: Pentraxin 3 ameliorates glucocorticoid-induced osteonecrosis of the femoral head via TLR4/NF-κB/FGF21 signaling axis.
Article Snippet: .. The primary antibodies including beta-Actin (1:1000 dilution, M20005F, Abmart), PTX3 (1:1000, 13797-1-AP, Proteintech), Bax (1:1000, T40051, Abmart), Bcl-2 (1:500, sc-509, Santa Cruz Biotechnology), Osteocalcin (1:1000 dilution, DF12303, Affinity), ALP (1:1000 dilution, DF6225, Affinity), Collagen I (1:1000 dilution, A24112, ABclonal), Runx2 (1:1000 AR TI CL E IN P RE SS ARTICLE IN PRESS dilution, AF5186, Affinity), OPN (1:200 dilution, AF0227, Affinity), phosphorylated p65 (pp65,1:1000 dilution, AF2006, Affinity), phosphorylated IκB alpha (p-IκB, 1:1000 dilution, 82349- 1-RR, Proteintech), Toll like receptor 4 (TLR4, 1:1000 dilution, AF7017, Affinity), Mutant myeloid differentiation primary response 88 (MyD88, 1:1000 dilution, AF5195, Affinity), p65 (1:1000 dilution, AF5006, Affinity), IκB alpha (1:1000 dilution, AF5002, Affinity) and FGF21 (1:1000 dilution, TC52978S, Abmart) diluted in the blocking buffer, were applied to the membrane and allowed to incubate overnight at 4°C to detect the target proteins. .. Following incubation with the appropriate anti-rabbit or anti-mouse secondary antibodies for 1 hour at 37°C, the protein bands were visualized using the Odyssey infrared imaging system (LI-COR® Biosciences, USA) and analyzed according to the Odyssey software manual instructions. β-actin served as the internal control.
Control:Article Title: Identification of Potential Therapeutic Targets for Sepsis Using Mendelian Randomization and Integrated eQTL/pQTL Analysis
Article Snippet: Equal protein amounts (approximately 20–40 μg per sample) were separated using SDS-PAGE gels and electrophoretically transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. Membranes underwent blocking with 5% skimmed milk in TBST for one hour at ambient temperature and were subsequently incubated overnight (4°C) with primary antibodies specific to BCL6 (ab33901), PTX3 (ab90806), IL7R (ab314106), BTN3A2 (Cat No. 15148-1-AP, Proteintech), LGALS1 (ab150427), and the internal control GAPDH (60004-1-Ig, Proteintech). .. Tissue samples and cells were lysed using RIPA buffer containing protease inhibitors, and protein concentrations were measured via a BCA assay kit (Beyotime, China).
Article Title: Identification of Potential Therapeutic Targets for Sepsis Using Mendelian Randomization and Integrated eQTL/pQTL Analysis
Article Snippet: Equal protein amounts (approximately 20–40 μg per sample) were separated using SDS-PAGE gels and electrophoretically transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. Membranes underwent blocking with 5% skimmed milk in TBST for one hour at ambient temperature and were subsequently incubated overnight (4°C) with primary antibodies specific to BCL6 (ab33901), PTX3 (ab90806), IL7R (ab314106), BTN3A2 (Cat No. 15148-1-AP, Proteintech), LGALS1 (ab150427), and the internal control GAPDH (60004-1-Ig, Proteintech). .. Following washing steps, the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Proteintech) at room temperature for one hour.
Mutagenesis:Article Title: Pentraxin 3 ameliorates glucocorticoid-induced osteonecrosis of the femoral head via TLR4/NF-κB/FGF21 signaling axis
Article Snippet: .. The primary antibodies including beta-Actin (1:1000 dilution, M20005F, Abmart), PTX3 (1:1000, 13797-1-AP, Proteintech), Bax (1:1000, T40051 , Abmart), Bcl-2 (1:500, sc-509, Santa Cruz Biotechnology), Osteocalcin (1:1000 dilution, DF12303, Affinity), ALP (1:1000 dilution, DF6225, Affinity), Collagen I (1:1000 dilution, A24112, ABclonal), Runx2 (1:1000 dilution, AF5186, Affinity), OPN (1:200 dilution, AF0227, Affinity), phosphorylated p65 (p-p65,1:1000 dilution, AF2006, Affinity), phosphorylated IκB alpha (p-IκB, 1:1000 dilution, 82349-1-RR, Proteintech), Toll like receptor 4 (TLR4, 1:1000 dilution, AF7017, Affinity), Mutant myeloid differentiation primary response 88 (MyD88, 1:1000 dilution, AF5195, Affinity), p65 (1:1000 dilution, AF5006, Affinity), IκB alpha (1:1000 dilution, AF5002, Affinity) and FGF21 (1:1000 dilution, TC52978S, Abmart) diluted in the blocking buffer, were applied to the membrane and allowed to incubate overnight at 4 °C to detect the target proteins. .. Following incubation with the appropriate anti-rabbit or anti-mouse secondary antibodies for 1 h at 37 °C, the protein bands were visualized using the Odyssey infrared imaging system (LI-COR® Biosciences, USA) and analyzed according to the Odyssey software manual instructions. β-actin served as the internal control.
Article Title: Pentraxin 3 ameliorates glucocorticoid-induced osteonecrosis of the femoral head via TLR4/NF-κB/FGF21 signaling axis.
Article Snippet: .. The primary antibodies including beta-Actin (1:1000 dilution, M20005F, Abmart), PTX3 (1:1000, 13797-1-AP, Proteintech), Bax (1:1000, T40051, Abmart), Bcl-2 (1:500, sc-509, Santa Cruz Biotechnology), Osteocalcin (1:1000 dilution, DF12303, Affinity), ALP (1:1000 dilution, DF6225, Affinity), Collagen I (1:1000 dilution, A24112, ABclonal), Runx2 (1:1000 AR TI CL E IN P RE SS ARTICLE IN PRESS dilution, AF5186, Affinity), OPN (1:200 dilution, AF0227, Affinity), phosphorylated p65 (pp65,1:1000 dilution, AF2006, Affinity), phosphorylated IκB alpha (p-IκB, 1:1000 dilution, 82349- 1-RR, Proteintech), Toll like receptor 4 (TLR4, 1:1000 dilution, AF7017, Affinity), Mutant myeloid differentiation primary response 88 (MyD88, 1:1000 dilution, AF5195, Affinity), p65 (1:1000 dilution, AF5006, Affinity), IκB alpha (1:1000 dilution, AF5002, Affinity) and FGF21 (1:1000 dilution, TC52978S, Abmart) diluted in the blocking buffer, were applied to the membrane and allowed to incubate overnight at 4°C to detect the target proteins. .. Following incubation with the appropriate anti-rabbit or anti-mouse secondary antibodies for 1 hour at 37°C, the protein bands were visualized using the Odyssey infrared imaging system (LI-COR® Biosciences, USA) and analyzed according to the Odyssey software manual instructions. β-actin served as the internal control.
Membrane:Article Title: Pentraxin 3 ameliorates glucocorticoid-induced osteonecrosis of the femoral head via TLR4/NF-κB/FGF21 signaling axis
Article Snippet: .. The primary antibodies including beta-Actin (1:1000 dilution, M20005F, Abmart), PTX3 (1:1000, 13797-1-AP, Proteintech), Bax (1:1000, T40051 , Abmart), Bcl-2 (1:500, sc-509, Santa Cruz Biotechnology), Osteocalcin (1:1000 dilution, DF12303, Affinity), ALP (1:1000 dilution, DF6225, Affinity), Collagen I (1:1000 dilution, A24112, ABclonal), Runx2 (1:1000 dilution, AF5186, Affinity), OPN (1:200 dilution, AF0227, Affinity), phosphorylated p65 (p-p65,1:1000 dilution, AF2006, Affinity), phosphorylated IκB alpha (p-IκB, 1:1000 dilution, 82349-1-RR, Proteintech), Toll like receptor 4 (TLR4, 1:1000 dilution, AF7017, Affinity), Mutant myeloid differentiation primary response 88 (MyD88, 1:1000 dilution, AF5195, Affinity), p65 (1:1000 dilution, AF5006, Affinity), IκB alpha (1:1000 dilution, AF5002, Affinity) and FGF21 (1:1000 dilution, TC52978S, Abmart) diluted in the blocking buffer, were applied to the membrane and allowed to incubate overnight at 4 °C to detect the target proteins. .. Following incubation with the appropriate anti-rabbit or anti-mouse secondary antibodies for 1 h at 37 °C, the protein bands were visualized using the Odyssey infrared imaging system (LI-COR® Biosciences, USA) and analyzed according to the Odyssey software manual instructions. β-actin served as the internal control.
Article Title: Pentraxin 3 ameliorates glucocorticoid-induced osteonecrosis of the femoral head via TLR4/NF-κB/FGF21 signaling axis.
Article Snippet: .. The primary antibodies including beta-Actin (1:1000 dilution, M20005F, Abmart), PTX3 (1:1000, 13797-1-AP, Proteintech), Bax (1:1000, T40051, Abmart), Bcl-2 (1:500, sc-509, Santa Cruz Biotechnology), Osteocalcin (1:1000 dilution, DF12303, Affinity), ALP (1:1000 dilution, DF6225, Affinity), Collagen I (1:1000 dilution, A24112, ABclonal), Runx2 (1:1000 AR TI CL E IN P RE SS ARTICLE IN PRESS dilution, AF5186, Affinity), OPN (1:200 dilution, AF0227, Affinity), phosphorylated p65 (pp65,1:1000 dilution, AF2006, Affinity), phosphorylated IκB alpha (p-IκB, 1:1000 dilution, 82349- 1-RR, Proteintech), Toll like receptor 4 (TLR4, 1:1000 dilution, AF7017, Affinity), Mutant myeloid differentiation primary response 88 (MyD88, 1:1000 dilution, AF5195, Affinity), p65 (1:1000 dilution, AF5006, Affinity), IκB alpha (1:1000 dilution, AF5002, Affinity) and FGF21 (1:1000 dilution, TC52978S, Abmart) diluted in the blocking buffer, were applied to the membrane and allowed to incubate overnight at 4°C to detect the target proteins. .. Following incubation with the appropriate anti-rabbit or anti-mouse secondary antibodies for 1 hour at 37°C, the protein bands were visualized using the Odyssey infrared imaging system (LI-COR® Biosciences, USA) and analyzed according to the Odyssey software manual instructions. β-actin served as the internal control.
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