psb603  (Tocris)

Journal: American Journal of Clinical and Experimental Urology

Article Title: Transmembrane prostatic acid phosphatase: a therapeutic target in advanced prostate cancer

doi: 10.62347/dziu5992

Figure Lengend Snippet: Figure 7. Adenosine receptor antagonist inhibited prostate xenografts in mice: VCaP cells were grown subcutaneously in SCID mice. Treatment with A2BR antagonist PSB (A) and A3R antagonist MRS1220 (B) inhibited tumor growth compared to untreated. Values represent Geometric Average ± SD, signifi cance as indicated by * = P < 0.05 as measured by Bonferroni’s multiple comparisons test, Control vs. Treatment at each timepoint.

Article Snippet: The adenosine receptor inhibitor treatment groups were as follows: (1) Control (PBS injection), (2) the A2BR antagonist PSB603 (Cat # 3198, Tocris Biosciences) 0.05 mg/ kg and (3) the A3R antagonist MRS1220 (Cat # 1217, Tocris Biosciences) 0.05 mg/kg, all given intraperitoneally three times weekly for 4 weeks.

Techniques: Control

Journal: Purinergic signalling

Article Title: Blockage of the adenosine A 2B receptor prevents cardiac fibroblasts overgrowth in rats with pulmonary arterial hypertension.

doi: 10.1007/s11302-023-09952-z

Figure Lengend Snippet: Fig. 7 Selective A2B receptor blockage with PSB603 (100 nM) attenuates NECA-induced overgrowth of cardiac fibroblasts (CFs) from the RV of MCT-treated rats. NECA (10 μM) with or without PSB603 (100 nM) was incorporated in culture media throughout the whole assay. The ordinates represent NECA- and/or PSB603-induced changes in cell growth (MTT assay, A) and type I collagen produc- tion (Sirius Red assay, B) compared to the control situation using the same cell batch in the absence of test drugs at culture days 7, 14, 21, and 28. Zero represents the similarity between the two values (drug vs. control); positive and negative values represent facilitation or inhi- bition of either cell growth or type I collagen production relative to control data obtained at the same time points. Each bar represents pooled data from 5 (CTRL) and 6 (MCT) animals; 3 to 4 replicas were performed for each experiment. The vertical bars represent SEM. *p < 0.05 (ANOVA, one-way analysis of variance) represents significant differences from control values obtained in the absence of test drugs; #p < 0.05 (ANOVA, one-way analysis of variance) repre- sents significant differences compared to the effect of NECA alone

Article Snippet: 8-[4-[4-(4-Chlorophenzyl)piperazide-1-sulfonyl)phenyl]]-1-propyl xanthine (PSB603) was from Tocris Cookson Inc. (Bristol, UK).

Techniques: MTT Assay, Control