protoscript ii first strand cdna synthesis kit (New England Biolabs)

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New England Biolabs protoscript ii first strand cdna synthesis kit
Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protoscript+kit/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pmc13141466-260-13-20
Average 99 stars, based on 1832 article reviews

protoscript ii first strand cdna synthesis kit - by Bioz Stars, 2026-09

99/100 stars

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Reverse Transcription:

Article Title: Regulation of pericyte metabolic reprogramming restricts the AKI to CKD transition.
Article Snippet: Background and aims: Acute kidney injury (AKI) is associated with high morbidity and mortality and is recognized as a long-term risk factor for progression to chronic kidney disease (CKD).. The AKI to CKD transition is characterized by interstitial fibrosis and the proliferation of collagen-secreting myofibroblasts.. Pericytes are the major source of myofibroblasts in kidney fibrosis.

Article Title: Hippo pathway activated by circulating reactive oxygen species mediates cardiac diastolic dysfunction after acute kidney injury.
Article Snippet: Abbreviation: AKI, Acute kidney injury; BCAA, Branched-chain amino acids; BUN, blood urea nitrogen; CRS, cardiorenal syndrome; DHE, Dihydroethidium; EF, ejection fraction; FS, fractional shortening; H&E, Hematoxylin and eosin; IRI, Ischemia-reperfusion injury; IVRT, isovolumic relaxation time; IVSd, interventricular septum thickness in diastole; IVSs, interventricular septum thickness in systole; LVIDd, left ventricle internal diameter in diastole; LVIDs, left ventricle internal diameter in systole; LVPWd, left ventricular posterior wall thickness in diastole; LVPWs, left ventricular posterior wall thickness in systole; MV DT, mitral valve descending time; PAS, Periodic acid–Schiff; ROS, Reactive oxygen species; SOD, Superoxide dismutase Jo ur na l P re -p ro of Journal Pre-proof 2 Acute kidney injury (AKI) can cause distal cardiac dysfunction; however, the underlying mechanism is unknown.. Oxidative stress is proved prominent in AKIinduced cardiac dysfunction, and a possible bridge role of oxidative-stress products in cardio-renal interaction has been reported.. Therefore, this study aimed to investigate the critical role of circulating reactive oxygen species (ROS) in mediating cardiac dysfunction after bilateral renal ischemia-reperfusion injury (IRI).

Real-time Polymerase Chain Reaction:

Sequencing:

Synthesized:

Article Title: The role of the atypical chemokine receptor CCRL2 in myelodysplastic syndrome and secondary acute myeloid leukemia
Article Snippet: .. In brief, total RNA was extracted by using the RNeasy Mini Kit (Qiagen, Valencia, CA), and complementary DNA was synthesized by using the ProtoScript kit (New England BioLabs). .. Quantitative real-time PCR was conducted by using sequence-specific primers (CCRL2 forward 5’-CTTCGAGAAAAACGTCTC-3’ and reverse 5’-CATCATATTCATCCTCTGGTG-3’, DNMT1 forward 5’-CGTAAAGAAGAATTATCCGAGG-3’ and reverse 5’-GTTTTCTAGACGTCCATTCAC-3’, PIM1 forward 5’-CTCTTCAGAATGTCAGGATC-3’ and reverse 5’-GGATGGTTCTGGATTTCTTC-3’, BCL2 forward 5’-GATTGTGGCCTTCTTTGAG-3’ and reverse 5’-GTTCCACAAAGGCATCC-3’, MCL1 forward 5’-TAGTTAAACAAGAGGCTGG-3’ and reverse 5’-ATAAACTGGTTTTGGTGGTG-3’, MYC forward 5’-TGAGGAGGAACAACAAGATG-3’ and reverse 5’-ATCCAGACTCTGACCTTTTG-3’ and GAPDH forward 5’-CTTTTGCGTCGCCAG-3’ and reverse 5’-TTGATGGCAACAATATCCA-3’) (Sigma-Aldrich), the Radian SYBR Green Lo-ROX qPCR Kit (Alkali Scientific, Fort Lauderdale, FL), and the CFX96 real-time PCR detection system (Bio-Rad).

Article Title: A SNP of miR-146a is involved in bladder cancer relapse by affecting the function of bladder cancer stem cells via the miR-146a signallings.
Article Snippet: In the next step, the host DNA in each isolated RNA sample was eliminated by subjecting the sample to a DNase treatment with a Heat & Run kit (ArcticZymes), and the isolated RNA was then inactivated for 5 minutes by heating to 80°C. .. Subsequently, first-strand cDNA corresponding to each RNA sample was synthesized using a Protoscript kit (New England Biolabs) following the recommendations on the kit manual. .. Finally, the miR-146a gene in each sample was amplified by real-time PCR and the genotypes of rs2910164 SNP located in miR146a were determined by direct sequencing using Illumina NextSeq 500 sequencer.

Article Title: MICA/B antibody induces macrophage-mediated immunity against acute myeloid leukemia
Article Snippet: NB4, C1498-MICB, and WEHI-3-MICB had messenger RNAs (mRNAs) isolated with RNeasy Plus Mini Kit (QIAGEN), which includes genomic DNA Eliminator Columns. .. Complementary DNA (cDNA) was synthesized with ProtoScript Kit (New England BioLabs), and polymerase chain reaction (PCR) performed with DreamTaq Green Master Mix (Thermo Fisher Scientific). ..

Polymerase Chain Reaction:

Control:

cDNA Synthesis:

Article Title: Genome-wide miRNAs profiles of pearl millet under contrasting high vapor pressure deficit reveal their functional roles in drought stress adaptations.
Article Snippet: The obtained sRNAs were sequentially ligated to 30 and 50 adapters using T4 RNA ligase 1 (NEB). .. The adapter-ligated sRNAs were used for cDNA synthesis using the ProtoScript kit (NEB). .. The cDNA was further purified and subjected to sequencing using the Illumina MiSeq platform.

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