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megalign program  (DNASTAR)


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    Structured Review

    DNASTAR megalign program
    Megalign Program, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 5966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/programming/MegAlign/pm42873425-62-6-8
    Average 99 stars, based on 5966 article reviews
    megalign program - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Software:

    Article Title: Molecular and Histopathological Findings of Giardia duodenalis in Rodents and Bats from the Atlantic Forest and Cerrado of Southeastern Brazil
    Article Snippet: PCR products were purified using the Wizard SV gel and PCR Clean-Up System kit (Promega, Madison, WI, USA), sequenced in both directions using the BigDye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA), and run on the ABI 3730 Sequencing Platform. .. The sequences were analyzed and edited using the SeqMan software 6 (DNASTAR software package, DNASTAR Inc., Madison, WI, USA) and compared with previously published sequences using the BLASTN software from the NCBI server (http://www.ncbi.nlm.nih.gov/BLAST, accessed on 15 May 2026). .. The consensus sequences were compared with reference sequences available in the National Center for Biotechnology Information (NCBI) database using the Basic Local Alignment Search Tool (BLAST) to determine sequence similarity.

    Article Title: Pathological injury and gastrointestinal microbiota changes in lambs infected with an Eimeria ovinoidalis isolate from Gansu, China.
    Article Snippet: Coccidiosis poses a serious threat to the sheep industry, with Eimeria ovinoidalis recognized as the primary causative agent.. However, quantitative analyses of the pathological damage induced by Eimeria ovinoidalis infection across the entire intestinal tract of sheep, together with investigations into the structural characteristics of the gastrointestinal microbiota, remain scarce.. In this study, Eimeria ovinoidalis was isolated and purified from the feces of naturally infected sheep in Gansu Province, China.

    Article Title: The dependence of Rev-mediated nuclear export of EIAV mRNA on Rev multimerization is modulated by mRNA length.
    Article Snippet: .. All rev sequences obtained in this study were organized and translated into amino acid sequences using the EditSeq tool in the Lasergene DNAStar 7.1 software. ..

    Article Title: Chitinase production and effects of land use change on fungal communities in the soil of the Brazilian Cerrado.
    Article Snippet: The resulting amplicons were subjected to Sanger sequencing at Instituto René Rachou (FIOCRUZ Minas). .. Sanger sequencing chromatograms were processed using DNASTAR Lasergene software to generate consensus ITS sequences. ..

    Article Title: Transient peak of p53 induction persists at low radiation doses in MCF-7 cells.
    Article Snippet: .. All primers were designed using the Primer designing tool from the National Library of Medicine NIH [21] and DNASTAR Lasergene 13 SeqBuilder software [22]. ..

    Article Title: High Lipid Yield and Efficient Biodiesel Production from Fusarium solani 102F Using Waste Eggshell as Biocatalyst
    Article Snippet: Resulting sequences were queried against the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov) using BLAST (version 2.17.0+). .. Phylogenetic relationships were inferred by aligning query sequences with reference sequences from GenBank using ClustalW implemented in MegAlign software version 5.05 (DNASTAR Inc., Madison, WI, USA). ..

    Article Title: Disk diffusion and ETEST (gradient diffusion) may produce false susceptible results for Pseudomonas aeruginosa from cystic fibrosis patients due to inducible colistin tolerance.
    Article Snippet: Processing of the P. aeruginosa GeneChip (Affymetrix) was performed at the Department of Clinical Biochemistry, Microarray Core Unit, Rigshospitalet, University of Copenhagen, Denmark. .. The gene expression analysis was done by ArrayStar v.5 Software (DNASTAR). ..

    Article Title: Functional characterization of compound heterozygous HLCS variants reveals exon skipping and impaired biotin responsiveness in holocarboxylase synthetase deficiency.
    Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof Holocarboxylase synthetase deficiency (HLCSD) is a rare inherited metabolic disorder caused by variants in the HLCS gene.. Functional characterization can aid the interpretation of rare HLCS variants.. Clinical, biochemical, genetic, transcriptional, functional, and structural analyses were performed, including urine organic acid analysis, targeted next-generation sequencing, Sanger validation, RT-PCR, cDNA sequencing, fibroblast propionyl-CoA carboxylase (PCC) activity before and after biotin treatment, and AlphaFold3-based structural modeling.

    Sequencing:

    Article Title: Pathological injury and gastrointestinal microbiota changes in lambs infected with an Eimeria ovinoidalis isolate from Gansu, China.
    Article Snippet: Coccidiosis poses a serious threat to the sheep industry, with Eimeria ovinoidalis recognized as the primary causative agent.. However, quantitative analyses of the pathological damage induced by Eimeria ovinoidalis infection across the entire intestinal tract of sheep, together with investigations into the structural characteristics of the gastrointestinal microbiota, remain scarce.. In this study, Eimeria ovinoidalis was isolated and purified from the feces of naturally infected sheep in Gansu Province, China.

    Article Title: Functional characterization of compound heterozygous HLCS variants reveals exon skipping and impaired biotin responsiveness in holocarboxylase synthetase deficiency.
    Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof Holocarboxylase synthetase deficiency (HLCSD) is a rare inherited metabolic disorder caused by variants in the HLCS gene.. Functional characterization can aid the interpretation of rare HLCS variants.. Clinical, biochemical, genetic, transcriptional, functional, and structural analyses were performed, including urine organic acid analysis, targeted next-generation sequencing, Sanger validation, RT-PCR, cDNA sequencing, fibroblast propionyl-CoA carboxylase (PCC) activity before and after biotin treatment, and AlphaFold3-based structural modeling.

    DNA Sequencing:

    Article Title: Chitinase production and effects of land use change on fungal communities in the soil of the Brazilian Cerrado.
    Article Snippet: The resulting amplicons were subjected to Sanger sequencing at Instituto René Rachou (FIOCRUZ Minas). .. Sanger sequencing chromatograms were processed using DNASTAR Lasergene software to generate consensus ITS sequences. ..

    Gene Expression:

    Article Title: Disk diffusion and ETEST (gradient diffusion) may produce false susceptible results for Pseudomonas aeruginosa from cystic fibrosis patients due to inducible colistin tolerance.
    Article Snippet: Processing of the P. aeruginosa GeneChip (Affymetrix) was performed at the Department of Clinical Biochemistry, Microarray Core Unit, Rigshospitalet, University of Copenhagen, Denmark. .. The gene expression analysis was done by ArrayStar v.5 Software (DNASTAR). ..

    Analysis:

    Article Title: Disk diffusion and ETEST (gradient diffusion) may produce false susceptible results for Pseudomonas aeruginosa from cystic fibrosis patients due to inducible colistin tolerance.
    Article Snippet: Processing of the P. aeruginosa GeneChip (Affymetrix) was performed at the Department of Clinical Biochemistry, Microarray Core Unit, Rigshospitalet, University of Copenhagen, Denmark. .. The gene expression analysis was done by ArrayStar v.5 Software (DNASTAR). ..



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