Journal: Oncology Letters

Article Title: Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma

doi: 10.3892/ol.2014.2356

Figure Lengend Snippet: Representative immunohistochemical streptavidin-peroxidase staining in extranodal natural killer/T-cell lymphoma (upper row) and rhinitis tissues (lower row). The positive cases of (A and D) programmed death 1, (B and E) PD-L1 and (C and F) PD-L2 (magnification, ×200). PD-L, programmed death ligand.

Article Snippet: The antigen retrieval was conducted in 0.01 mol/l citrate (pH 6.0) and the slides were incubated overnight with rabbit anti-human PD-L1 polyclonal antibody (1:120; Proteintech, Chicago, IL, USA), rabbit anti-human PD-L2 polyclonal antibody (1:150) and mouse anti-human PD-1 monoclonal antibody (mAb; 1:100) (both ZSGB-BIO, Beijing, China) and phosphate-buffered saline was used as a blank control.

Techniques: Immunohistochemical staining, Staining

Journal: Oncology Letters

Article Title: Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma

doi: 10.3892/ol.2014.2356

Figure Lengend Snippet: PD-1 expression in (A) CD4 + and (B) CD8 + T-cell subsets in 20 ENKL patients was significantly increased compared with that in 10 HVs (P<0.05). Representative PD-1 expression in (C) CD4 + and (D) CD8 + T-cell subsets in six ENKL patients was (E) downregulated with chemotherapy. (F) T-helper cell type 1 cytokine (IL-2 and IFN-γ) mean production levels in the serum of 20 ENKL patients were significantly lower than those in 10 HVs (P<0.05). PD1, programmed death 1; ENKL, extranodal natural killer/T-cell lymphoma; HVs, healthy volunteers; IL-2 interleukin 2; IFN-γ, interferon γ.

Article Snippet: The antigen retrieval was conducted in 0.01 mol/l citrate (pH 6.0) and the slides were incubated overnight with rabbit anti-human PD-L1 polyclonal antibody (1:120; Proteintech, Chicago, IL, USA), rabbit anti-human PD-L2 polyclonal antibody (1:150) and mouse anti-human PD-1 monoclonal antibody (mAb; 1:100) (both ZSGB-BIO, Beijing, China) and phosphate-buffered saline was used as a blank control.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma

doi: 10.3892/ol.2014.2356

Figure Lengend Snippet: (A) Purity of CD8 + T cells separated by magnetic-activated cell sorting was 99%. (B) Purity of CD8 + PD-1 + T cells was 96.2% following the stimulation of allogeneic CD8 + T cells with phytohemagglutinin for 48 h. (C) SNK-6 cells were used as the control group and, following the coculture of SNK-6 cells and CD8 + T cells for 72 h, a significant inhibitory effect of PD-L1 on allogeneic CD8 + T-helper type 1 cytokine (IL-2 and IFN-γ) secretion was observed; (A and B) P<0.05. (D) CD8 + T-cell apoptosis in groups A and B was not altered significantly compared with activated CD8 + T cells at 72 h (P>0.05). (E) SNK-6 cells were used as the control group and cells harvested at 0, 24, 48 and 72 h were analyzed by flow cytometry gating CFSE + events. The proliferation index was not significantly different among the groups (P>0.05). PD-1, programme death 1; PD-L. programmed death ligand; IL-2 interleukin 2; IFN-γ, interferon γ; CFSE, carboxy-fluorescein succinimidyl ester.

Article Snippet: The antigen retrieval was conducted in 0.01 mol/l citrate (pH 6.0) and the slides were incubated overnight with rabbit anti-human PD-L1 polyclonal antibody (1:120; Proteintech, Chicago, IL, USA), rabbit anti-human PD-L2 polyclonal antibody (1:150) and mouse anti-human PD-1 monoclonal antibody (mAb; 1:100) (both ZSGB-BIO, Beijing, China) and phosphate-buffered saline was used as a blank control.

Techniques: FACS, Flow Cytometry

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: PDCD2 expression in pan-cancer. (A) Examining the TIMER database uncovers PDCD2 expression in pan-cancers. (B) Pan-cancer analysis of PDCD2 expression in TCGA/GTEx. (C) Data from the UALCAN database on PDCD2 protein in pan-cancer analysis. ns, P > 0.05; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques: Expressing

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: The PDCD2 prognostic values in pan-cancer. (A) The impact of PDCD2 expression on the overall survival rate of patients with ACC, GBMLGG, LIHC, KIRC, or SARC. (B) The disease-specific survival for PDCD2 in ACC, GBMLGG, and UCEC. (C) The progress-free survival for PDCD2 in ACC, GBMLGG, HNSC, LIHC, OV, and UCEC.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques: Expressing

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: ROC curve analysis of PDCD2 in pan-cancer. ROC curve analysis of PDCD2 in CHOL, COAD, LIHC, STAD, PAAD, and READ (A) , ESAD, ESCA, and LUSC (B) , GBMLGG, GBM, and LGG (C) , KICH, TGCT, and UCS (D) . TPR, true positive rate; FPR, false positive rate.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques:

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: Mutational analysis of PDCD2. (A) Hot spots of mutation of PDCD2. (B) A summary of PDCD2 mutation types and their distribution among different cancers. (C) Correlation of PDCD2 CNV with mRNA expression in pan-cancer. ns, P > 0.05; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques: Mutagenesis, Expressing

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: PDCD2 is correlated with clinical characteristics in GBMLGG. The PDCD2 was up-regulated in GBMLGG (A) , and associated with WHO grade, IDH status, primary therapy outcome, age, race, histological type, OS event, DSS event, and PFI event (B – J) (data from TCGA). PDCD2 is correlated with clinical characteristics in glioma, including the WHO grade, IDH mutation status, 1p/19q co-deletion status, IDH mutation status & 1p/19q co-deletion status, gender, and histology (K–P) (data from CGGA). G2, grade II; G3, grade III; G4, grade IV; PR, partial response; CR, complete response; PD, progressive disease; OS, overall survival; DSS, disease-specific survival; PFS, progression-free survival; WT, wild type; Mut, mutant. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques: Mutagenesis

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: PDCD2 is correlated with prognosis in GBMLGG. The correlation between PDCD2 and OS in 1p/19q co-deletion status (A) , gender (both female and male) (B, C) , race (white) (D) , age (E, F) , and histological type (G) of GBMLGG.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques:

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: Univariate and multivariate Cox regression analyses of clinical characteristics associated with OS of glioma.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques:

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: The ability of PDCD2 to predict the GBMLGG patients' prognosis. Construction and evaluation of nomogram used for predicting OS (A) , DFS (B) , and PFS (C) of GBMLGG patients. The calibration curve and Hosmer–Lemeshow test of nomograms in the TCGA-GBMLGG cohort for OS (D) , DFS (E) , and PFS (F) .

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques:

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: Interaction network of PDCD2. The network of PDCD2 at the protein (A) and gene (B) levels in pan-cancer. Correlation between PDCD2 expression and the expression of PDCD2L, NFKBIB, PRS2, and NFKB1 in GBMLGG (C) . (D, E) Expression and prognosis of PDCD2L, NFKBIB, PRS2, and NFKB1 in GBMLGG.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques: Expressing

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: KEGG and GO enrichment analysis for PDCD2. The top 50 genes positively (A) and negatively (B) correlated with PDCD2 are shown in the heat map. (C, D) KEGG pathway (C) and biology process analysis (D) of PDCD2.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques:

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: GSEA enrichment PDCD2-related signaling pathway in GBMLGG.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques:

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: KEGG enrichment PDCD2-related signaling pathway in GBMLGG.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques:

Journal: Genes & Diseases

Article Title: PDCD2 as a prognostic biomarker in glioma correlates with malignant phenotype

doi: 10.1016/j.gendis.2023.101106

Figure Lengend Snippet: PDCD2 promotes GBMLGG progression. (A, B) To determine the levels of PDCD2 expression in U251 and U87 cells transfected with siRNA targeting PDCD2, qPCR and Western blotting were employed. (C) Cell growth ability was assessed using CCK8 assays at the specified time intervals in U251 and U87 cells transfected with PDCD2 siRNAs. (D) PDCD2 knockdown inhibited colony formation of both U251 and U87 cells. (E, F) The migration and invasion of U251 and U87 cells were found to be inhibited by PDCD2 knockdown, as demonstrated by the transwell migration and invasion assays (scale bars = 200 μm). U87 cells were injected subcutaneously into nude mice to establish a xenograft model. Following the development of the tumor, intertumoral injection of si-PDCD2 or NC was performed ( n = 3/group). (G – I) Representative pictures and tumor growth chart at 16 days post-injection of si-PDCD2 or NC in GBMLGG xenograft model. (J) The weight of the xenograft tumor was measured and graphed 16 days after the injection. (K) IHC staining was used to examine the alterations in the levels of PDCD2 and Ki-67 in xenograft tumors. Representative images are shown (scale bars = 20 μm). The data were presented as mean ± standard deviation of three independent experiments. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: The technique of immunohistochemistry (IHC) was carried out following the methods described earlier., The xenograft sections of nude mice were treated with primary antibodies against PDCD2 (Proteintech, 10725-1-AP) and Ki67 (sc-23900, Santa Cruz) for overnight incubation at 4 °C.

Techniques: Expressing, Transfection, Western Blot, Knockdown, Migration, Injection, Immunohistochemistry, Standard Deviation