Journal: Frontiers in Pharmacology
Article Title: Exercise ameliorates hepatic lipid accumulation via upregulating serum PRL and activating hepatic PRLR-mediated JAK2/STAT5 signaling pathway in NAFLD mice
doi: 10.3389/fphar.2025.1647231
Figure Lengend Snippet: Expression of PRL/PRLR signaling pathway related proteins in liver tissue, and expression levels of PRLR (A) , JAK2 (B) , p-JAK2 (C) , STAT5a (D) , and p-STAT5 (E) in liver tissue of mice in each group.
Article Snippet: The PVDF membrane was blocked with 5% bovine serum albumin (BSA) at room temperature for 1 h and then incubated overnight at 4°C with gentle shaking in the presence of the following primary rabbit monoclonal antibodies: total PRLR (1:500 dilution, ER 1915-43, Huabio), JAK2 (1:1000 dilution, ET1607-35, Huabio), phosphorylated JAK2 (p-JAK2; 1:1000 dilution, ET1607-34, Huabio), STAT5a (1:1000 dilution, ET1610-58, Huabio), phosphorylated STAT5 (p-STAT5; 1:1000 dilution, ET1610-48, Huabio), and β-actin (1:100,000 dilution, AC026, Abclonal).
Techniques: Expressing