Review



primer3plus  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Qiagen primer3plus
    Primer3plus, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer3plus/QIAGEN+Multiplex+PCR+Kit+x+%E2%80%8B%E2%80%8B100u/pm41375073-74-25-40
    Average 95 stars, based on 277 article reviews
    primer3plus - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Reverse Transcription:

    Article Title: Processed Transcript Insertion as a Novel Germline Mutational Mechanism in BRCA1 -Associated Hereditary Breast Cancer.
    Article Snippet: RNA was isolated either from peripheral blood with the Tempus Spin RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) or short-term–cultured peripheral blood mononuclear cells (PBMC) by the miRNeasy kit (103873, QIAGEN, Hilden, Germany), adhering to the protocols. .. First-strand reverse transcription was carried out by ProtoScript II Reverse Transcriptase (cat. no. E6560, New England Biolabs, Ipswich, MA, USA). cDNA primers were designed by Primer3Plus (https://www.primer3plus.com, accessed on 16 October 2023 and RT-PCR reactions were carried out with the QIAGEN Multiplex PCR Kit (cat. no. 206143, QIAGEN, Hilden, Germany). .. Amplification products were visualized on 1% agarose gel next to Hyper Ladder 1kb DNA sizing standard (cat. no. 33053, Bioline, London, UK) and subsequently sequenced by the conventional Sanger sequencing method on the ABI3130 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: Processed Transcript Insertion as a Novel Germline Mutational Mechanism in BRCA1 -Associated Hereditary Breast Cancer
    Article Snippet: RNA was isolated either from peripheral blood with the Tempus Spin RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) or short-term–cultured peripheral blood mononuclear cells (PBMC) by the miRNeasy kit (103873, QIAGEN, Hilden, Germany), adhering to the protocols. .. First-strand reverse transcription was carried out by ProtoScript II Reverse Transcriptase (cat. no. E6560, New England Biolabs, Ipswich, MA, USA). cDNA primers were designed by Primer3Plus ( https://www.primer3plus.com , accessed on 16 October 2023 and RT-PCR reactions were carried out with the QIAGEN Multiplex PCR Kit (cat. no. 206143, QIAGEN, Hilden, Germany). .. Amplification products were visualized on 1% agarose gel next to Hyper Ladder 1kb DNA sizing standard (cat. no. 33053, Bioline, London, UK) and subsequently sequenced by the conventional Sanger sequencing method on the ABI3130 Genetic Analyzer (Thermo Fisher Scientific).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Processed Transcript Insertion as a Novel Germline Mutational Mechanism in BRCA1 -Associated Hereditary Breast Cancer.
    Article Snippet: RNA was isolated either from peripheral blood with the Tempus Spin RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) or short-term–cultured peripheral blood mononuclear cells (PBMC) by the miRNeasy kit (103873, QIAGEN, Hilden, Germany), adhering to the protocols. .. First-strand reverse transcription was carried out by ProtoScript II Reverse Transcriptase (cat. no. E6560, New England Biolabs, Ipswich, MA, USA). cDNA primers were designed by Primer3Plus (https://www.primer3plus.com, accessed on 16 October 2023 and RT-PCR reactions were carried out with the QIAGEN Multiplex PCR Kit (cat. no. 206143, QIAGEN, Hilden, Germany). .. Amplification products were visualized on 1% agarose gel next to Hyper Ladder 1kb DNA sizing standard (cat. no. 33053, Bioline, London, UK) and subsequently sequenced by the conventional Sanger sequencing method on the ABI3130 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: Processed Transcript Insertion as a Novel Germline Mutational Mechanism in BRCA1 -Associated Hereditary Breast Cancer
    Article Snippet: RNA was isolated either from peripheral blood with the Tempus Spin RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) or short-term–cultured peripheral blood mononuclear cells (PBMC) by the miRNeasy kit (103873, QIAGEN, Hilden, Germany), adhering to the protocols. .. First-strand reverse transcription was carried out by ProtoScript II Reverse Transcriptase (cat. no. E6560, New England Biolabs, Ipswich, MA, USA). cDNA primers were designed by Primer3Plus ( https://www.primer3plus.com , accessed on 16 October 2023 and RT-PCR reactions were carried out with the QIAGEN Multiplex PCR Kit (cat. no. 206143, QIAGEN, Hilden, Germany). .. Amplification products were visualized on 1% agarose gel next to Hyper Ladder 1kb DNA sizing standard (cat. no. 33053, Bioline, London, UK) and subsequently sequenced by the conventional Sanger sequencing method on the ABI3130 Genetic Analyzer (Thermo Fisher Scientific).

    Multiplex Assay:

    Article Title: Processed Transcript Insertion as a Novel Germline Mutational Mechanism in BRCA1 -Associated Hereditary Breast Cancer.
    Article Snippet: RNA was isolated either from peripheral blood with the Tempus Spin RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) or short-term–cultured peripheral blood mononuclear cells (PBMC) by the miRNeasy kit (103873, QIAGEN, Hilden, Germany), adhering to the protocols. .. First-strand reverse transcription was carried out by ProtoScript II Reverse Transcriptase (cat. no. E6560, New England Biolabs, Ipswich, MA, USA). cDNA primers were designed by Primer3Plus (https://www.primer3plus.com, accessed on 16 October 2023 and RT-PCR reactions were carried out with the QIAGEN Multiplex PCR Kit (cat. no. 206143, QIAGEN, Hilden, Germany). .. Amplification products were visualized on 1% agarose gel next to Hyper Ladder 1kb DNA sizing standard (cat. no. 33053, Bioline, London, UK) and subsequently sequenced by the conventional Sanger sequencing method on the ABI3130 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: Processed Transcript Insertion as a Novel Germline Mutational Mechanism in BRCA1 -Associated Hereditary Breast Cancer
    Article Snippet: RNA was isolated either from peripheral blood with the Tempus Spin RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) or short-term–cultured peripheral blood mononuclear cells (PBMC) by the miRNeasy kit (103873, QIAGEN, Hilden, Germany), adhering to the protocols. .. First-strand reverse transcription was carried out by ProtoScript II Reverse Transcriptase (cat. no. E6560, New England Biolabs, Ipswich, MA, USA). cDNA primers were designed by Primer3Plus ( https://www.primer3plus.com , accessed on 16 October 2023 and RT-PCR reactions were carried out with the QIAGEN Multiplex PCR Kit (cat. no. 206143, QIAGEN, Hilden, Germany). .. Amplification products were visualized on 1% agarose gel next to Hyper Ladder 1kb DNA sizing standard (cat. no. 33053, Bioline, London, UK) and subsequently sequenced by the conventional Sanger sequencing method on the ABI3130 Genetic Analyzer (Thermo Fisher Scientific).

    Polymerase Chain Reaction:

    Article Title: Processed Transcript Insertion as a Novel Germline Mutational Mechanism in BRCA1 -Associated Hereditary Breast Cancer.
    Article Snippet: RNA was isolated either from peripheral blood with the Tempus Spin RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) or short-term–cultured peripheral blood mononuclear cells (PBMC) by the miRNeasy kit (103873, QIAGEN, Hilden, Germany), adhering to the protocols. .. First-strand reverse transcription was carried out by ProtoScript II Reverse Transcriptase (cat. no. E6560, New England Biolabs, Ipswich, MA, USA). cDNA primers were designed by Primer3Plus (https://www.primer3plus.com, accessed on 16 October 2023 and RT-PCR reactions were carried out with the QIAGEN Multiplex PCR Kit (cat. no. 206143, QIAGEN, Hilden, Germany). .. Amplification products were visualized on 1% agarose gel next to Hyper Ladder 1kb DNA sizing standard (cat. no. 33053, Bioline, London, UK) and subsequently sequenced by the conventional Sanger sequencing method on the ABI3130 Genetic Analyzer (Thermo Fisher Scientific).

    Article Title: Processed Transcript Insertion as a Novel Germline Mutational Mechanism in BRCA1 -Associated Hereditary Breast Cancer
    Article Snippet: RNA was isolated either from peripheral blood with the Tempus Spin RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) or short-term–cultured peripheral blood mononuclear cells (PBMC) by the miRNeasy kit (103873, QIAGEN, Hilden, Germany), adhering to the protocols. .. First-strand reverse transcription was carried out by ProtoScript II Reverse Transcriptase (cat. no. E6560, New England Biolabs, Ipswich, MA, USA). cDNA primers were designed by Primer3Plus ( https://www.primer3plus.com , accessed on 16 October 2023 and RT-PCR reactions were carried out with the QIAGEN Multiplex PCR Kit (cat. no. 206143, QIAGEN, Hilden, Germany). .. Amplification products were visualized on 1% agarose gel next to Hyper Ladder 1kb DNA sizing standard (cat. no. 33053, Bioline, London, UK) and subsequently sequenced by the conventional Sanger sequencing method on the ABI3130 Genetic Analyzer (Thermo Fisher Scientific).



    Similar Products

    86
    Sangon Biotech primer3plus web tool
    Primer3plus Web Tool, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer3plus/primer3plus/10__1127_slash_entomologia_slash_4176-45-6-14
    Average 86 stars, based on 1 article reviews
    primer3plus web tool - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Sangon Biotech primer3plus
    Primer3plus, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer3plus/primer3plus/pmc12914535-45-6-13
    Average 86 stars, based on 1 article reviews
    primer3plus - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    99
    Roche primer3plus
    Primer3plus, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer3plus/LightCycler+480+System/pmc13066512-300-10-26
    Average 99 stars, based on 1 article reviews
    primer3plus - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    Biotechnology Information primer3plus software
    Primer3plus Software, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer3plus/primer3/pm41812550-78-27-50
    Average 86 stars, based on 1 article reviews
    primer3plus software - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    95
    Qiagen primer3plus
    Primer3plus, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer3plus/QIAGEN+Multiplex+PCR+Kit+x+%E2%80%8B%E2%80%8B100u/pm41375073-74-25-40
    Average 95 stars, based on 1 article reviews
    primer3plus - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Benchling Inc primer3plus
    Primer3plus, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer3plus/primer3+tool/pmc13069148-55-7-22
    Average 86 stars, based on 1 article reviews
    primer3plus - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    90
    Eurofins primer3plus
    Primer3plus, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primer3plus/primer3plus/pm40646820-66-17-23
    Average 90 stars, based on 1 article reviews
    primer3plus - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results