primaquine  (MedChemExpress)

Journal: Medical Oncology (Northwood, London, England)

Article Title: A combination of artemisinin, moxidectin, and doxorubicin drugs can selectively and efficiently induce apoptosis in acute lymphoblastic and chronic myeloid leukemia cells in vitro and ex vivo

doi: 10.1007/s12032-026-03320-8

Figure Lengend Snippet: Antimalarial, anthelmintic, and antibiotic drugs affect the cell cycle and increase SubG1 phase in K562 cells. Representative histogram and quantitative data showing the SubG1, G1, S and G2/M positive populations in K562 cells treated with 0, 1, 10 and 100 µM artemisinin (AM; A-B ), chloroquine (CQ; C-D ), primaquine (PQ; E-F ), mefloquine (MQ; G-H ), ivermectin (IM; I-J ), moxidectin (MD; K-L ), doxorubicin (DR; M-N ), minocycline (MC; O-P ), N, N,N’,N’-Tetrakis(2-pyridylmethyl)ethylenediamine (TPEN; Q-R ) and 0, 1, 3, 5 and 10 µM Arsenic trioxide (As 2 O 3 ; S-T ) evaluated with propidium iodide (PI) staining. Histograms represent 1 out of 3 independent experiments and quantitative numbers (0–100%) represent the PI positive mean percentage of three independent experiments ( n = 3). Statistically significance at * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: Finally, both cell lines, PBL or BM cells were seeded at 1× 10 6 cells/cm 2 and incubated with 0, 1, 10 and 100 μM Artemisinin (Cat. HY-B0094; MedChemExpress, Monmouth Junction, NJ, USA), Chloroquine (Cat. HY-17589 A; MedChemExpress, Monmouth Junction, NJ, USA), Mefloquine (Cat. HY-17437 A; MedChemExpress, Monmouth Junction, NJ, USA), Primaquine (Cat. HY-12651 A; MedChemExpress, Monmouth Junction, NJ, USA), Ivermectin (Cat. HY-15310; MedChemExpress, Monmouth Junction, NJ, USA), Moxidectin (Cat. HY-B0777; MedChemExpress, Monmouth Junction, NJ, USA), Minocycline (Cat. M9511, Sigma-Aldrich, Saint Louis, MO, USA), TPEN (N, N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine; Cat. P4413 Sigma-Aldrich, Saint Louis, MO, USA) and doxorubicin (Cat. 44583, Sigma-Aldrich, Saint Louis, MO, USA) or with 0, 1, 3, 5 and 10 μM Arsenic trioxide (Cat. A1010, Sigma-Aldrich, Saint Louis, MO, USA) for 24 h at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Staining

Journal: Medical Oncology (Northwood, London, England)

Article Title: A combination of artemisinin, moxidectin, and doxorubicin drugs can selectively and efficiently induce apoptosis in acute lymphoblastic and chronic myeloid leukemia cells in vitro and ex vivo

doi: 10.1007/s12032-026-03320-8

Figure Lengend Snippet: Antimalarial, antiparasitic, and antibiotic drugs induce the dissipation of mitochondrial membrane potential (ΔΨm) in K562 cells. Representative histogram and quantitative data showing the MitoTracker ® (MT) low populations in K562 cells treated with 0, 1, 10 and 100 µM artemisinin (AM; A and B ), chloroquine (CQ; C and D ), primaquine (PQ; E and F ), mefloquine (MQ; G and H ), ivermectin (IM; I and J ), moxidectin (MD; K and L ), doxorubicin (DR; M and N) , minocycline (MC; O and P) , TPEN ( Q and R ) and 0, 1, 3, 5 and 10 µM As 2 O 3 ( S and T ). Histograms represent 1 out of 3 independent experiments and quantitative numbers (0–100%) represent the MT-low mean percentage of three independent experiments (black dots in bars). Statistically significance at * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: Finally, both cell lines, PBL or BM cells were seeded at 1× 10 6 cells/cm 2 and incubated with 0, 1, 10 and 100 μM Artemisinin (Cat. HY-B0094; MedChemExpress, Monmouth Junction, NJ, USA), Chloroquine (Cat. HY-17589 A; MedChemExpress, Monmouth Junction, NJ, USA), Mefloquine (Cat. HY-17437 A; MedChemExpress, Monmouth Junction, NJ, USA), Primaquine (Cat. HY-12651 A; MedChemExpress, Monmouth Junction, NJ, USA), Ivermectin (Cat. HY-15310; MedChemExpress, Monmouth Junction, NJ, USA), Moxidectin (Cat. HY-B0777; MedChemExpress, Monmouth Junction, NJ, USA), Minocycline (Cat. M9511, Sigma-Aldrich, Saint Louis, MO, USA), TPEN (N, N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine; Cat. P4413 Sigma-Aldrich, Saint Louis, MO, USA) and doxorubicin (Cat. 44583, Sigma-Aldrich, Saint Louis, MO, USA) or with 0, 1, 3, 5 and 10 μM Arsenic trioxide (Cat. A1010, Sigma-Aldrich, Saint Louis, MO, USA) for 24 h at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Membrane

Journal: Medical Oncology (Northwood, London, England)

Article Title: A combination of artemisinin, moxidectin, and doxorubicin drugs can selectively and efficiently induce apoptosis in acute lymphoblastic and chronic myeloid leukemia cells in vitro and ex vivo

doi: 10.1007/s12032-026-03320-8

Figure Lengend Snippet: Antimalarial, antiparasitic, and antibiotic drugs induce the activation of the protease CASPASE 3 into cleaved caspase 3 (CC3) in K562 cells. Representative histogram and quantitative data showing the CC3 positive populations in K562 cells treated with 0, 1, 10 and 100 µM artemisinin (AM; A and B ), chloroquine (CQ; C and D ), primaquine (PQ; E and F ), mefloquine (MQ; G and H ), ivermectin (IM; I and J ), moxidectin (MD; K and L ), doxorubicin (DR; M and N ), minocycline (MC; O and P ), TPEN ( Q and R ) and 0, 1, 3, 5 and 10 µM As 2 O 3 ( S and T ). Histograms represent 1 out of 3 independent experiments and quantitative numbers (0–100%) represent the mean percentage CC3 determination of three independent experiments (black dots in bars). Statistically significance at * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: Finally, both cell lines, PBL or BM cells were seeded at 1× 10 6 cells/cm 2 and incubated with 0, 1, 10 and 100 μM Artemisinin (Cat. HY-B0094; MedChemExpress, Monmouth Junction, NJ, USA), Chloroquine (Cat. HY-17589 A; MedChemExpress, Monmouth Junction, NJ, USA), Mefloquine (Cat. HY-17437 A; MedChemExpress, Monmouth Junction, NJ, USA), Primaquine (Cat. HY-12651 A; MedChemExpress, Monmouth Junction, NJ, USA), Ivermectin (Cat. HY-15310; MedChemExpress, Monmouth Junction, NJ, USA), Moxidectin (Cat. HY-B0777; MedChemExpress, Monmouth Junction, NJ, USA), Minocycline (Cat. M9511, Sigma-Aldrich, Saint Louis, MO, USA), TPEN (N, N,N′,N′-Tetrakis(2-pyridylmethyl)ethylenediamine; Cat. P4413 Sigma-Aldrich, Saint Louis, MO, USA) and doxorubicin (Cat. 44583, Sigma-Aldrich, Saint Louis, MO, USA) or with 0, 1, 3, 5 and 10 μM Arsenic trioxide (Cat. A1010, Sigma-Aldrich, Saint Louis, MO, USA) for 24 h at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Activation Assay