Journal: Brazilian Journal of Medical and Biological Research
Article Title: LTF regulates glioblastoma progression and temozolomide resistance via the NF-κB signaling pathway
doi: 10.1590/1414-431X2025e13821
Figure Lengend Snippet: Lactotransferrin (LTF) competitively bound p65. A , Phosphorylation levels of p65 in control or LTF-depleted glioblastoma (GBM) cells was analyzed by western blot. B , U251 cells were transfected with LTF with or without four phosphatases. Phosphorylation levels of p65 in control or LTF-depleted GBM cells were analyzed by western blot. C , Immunoprecipitates were prepared with anti-LTF antibody. p65 and PP2A expression in immunoprecipitates was measured by western blot. D , Control and LTF-depleted cells were transfected with Flag-p65. Immunoprecipitates were prepared with anti-Flag antibody. PP2A expression in immunoprecipitates was measured by western blot. E , Schematic representation of Flag-tagged full-length LTF (#1) and various deletion mutants (#2-4). F , Immunoprecipitation and western blot assays using Flag antibody showing the interaction between p65 and LTF protein in U251 cells transfected with a series of truncations of Flag-tagged LTF. G , Schematic representation of Flag-tagged full-length p65 (#1) and various deletion mutants (#2-5). H , Immunoprecipitation and western blot assays using Flag antibody showing the interaction between p65 and LTF protein in U251 cells transfected with a series truncations of Flag-tagged p65.
Article Snippet: Antibodies against LTF (10933-1-AP), Cyclin E1 (11554-1-AP), Cyclin D1 (26939-1-AP), E-cadherin (20874-1-AP), N-cadherin (22018-1-AP), γ-H2AX (29380-1-AP), PP2A (13482-1-AP), GAPDH (10494-1-AP), and H3 (17168-1-AP) were from Proteintech (China).
Techniques: Phospho-proteomics, Control, Western Blot, Transfection, Expressing, Immunoprecipitation
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