pparα  (Proteintech)

Journal: The Journal of Biological Chemistry

Article Title: Neurodevelopmental disease-causing variants in choline kinase CHKA gene couple phosphatidylcholine synthesis to oxidative stress damage and disease etiology

doi: 10.1016/j.jbc.2025.110983

Figure Lengend Snippet: Biallelic CHKA variants (p.Pro194Ser or P.Arg141Trp) are associated with altered expression of key lipid metabolism regulators . A–B , quantitative PCR analysis of CHKA , CHKB , PPARA , PPARB and CPT1B mRNA levels in fibroblasts carrying biallelic CHKA variants. Control is the mean of three different normal human skin fibroblast lines. C , Western blot analysis of PPARA, PPARB, and CPT1B protein expression. D , quantification of PPARA, PPARB, and CPT1B western blots relative to GAPDH. Results are the mean ± SD from three independent experiments. Statistical significance was determined by one-way ANOVA followed by Tukey’s test for multiple comparisons; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001.

Article Snippet: Quantitative real-time PCR was performed on a Bio-Rad CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using TaqMan Fast Advanced Master Mix (Cat. no. 4444557, ThermoFisher Scientific), and TaqMan Gene Expression Assays (Cat. no. 4331182, ThermoFisher Scientific) for CHKA (RRID: Hs00957878_m1), CHKB (RRID: Hs01925200_s1), CNSK2A2 (RRID: Hs00751002_s1), CPT1B (RRID: Hs03046298_s1), PPARA (RRID: Hs00947536_m1), and PPARB/D (RRID: Hs04187066_g1).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Western Blot

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: The protective effect of probiotic therapy on gut microbiota and the activation of ω-oxidation after ovarian cancer surgery

doi: 10.1007/s00432-025-06398-1

Figure Lengend Snippet: Expression of ω-oxidation-related molecules in mice after probiotic treatment following ovarian cancer surgery. A – C qPCR analysis of PPARα, CYP4F3, and CYP4A10 mRNA expression in mouse intestinal tissues. D Western blot detection of PPARα, CYP4F3, and CYP4A10 protein expression. E Measurement of MDA content in mouse intestinal tissues. F Flow cytometry analysis of ROS levels in mouse intestinal tissues. vs. Control, *** P < 0.001, **** P < 0.0001; vs. Model Group, # P < 0.05, ### P < 0.001, n = 5. Model Group: ovarian cancer surgery without probiotic treatment; Probiotic Group: ovarian cancer surgery with probiotic treatment

Article Snippet: Membranes were blocked with 5% non-fat milk at room temperature for 2 h. Subsequently, membranes were incubated overnight at 4 °C with primary antibodies against PPARα (Proteintech, 66826-1-Ig), CYP4F3 (Proteintech, 19701-1-AP), and CYP4A10 (Abcam, ab3573) diluted 1:1000 in blocking buffer.

Techniques: Expressing, Western Blot, Flow Cytometry, Control

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: The protective effect of probiotic therapy on gut microbiota and the activation of ω-oxidation after ovarian cancer surgery

doi: 10.1007/s00432-025-06398-1

Figure Lengend Snippet: Expression of ω-oxidation-related molecules in mice after probiotic treatment following ovarian cancer surgery. A – C qPCR analysis of PPARα, CYP4F3, and CYP4A10 mRNA expression in mouse intestinal tissues. D Western blot detection of PPARα, CYP4F3, and CYP4A10 protein expression. E Measurement of MDA content in mouse intestinal tissues. F Flow cytometry analysis of ROS levels in mouse intestinal tissues. vs. Control, *** P < 0.001, **** P < 0.0001; vs. Model Group, # P < 0.05, ### P < 0.001, n = 5. Model Group: ovarian cancer surgery without probiotic treatment; Probiotic Group: ovarian cancer surgery with probiotic treatment

Article Snippet: Membranes were blocked with 5% non-fat milk at room temperature for 2 h. Subsequently, membranes were incubated overnight at 4 °C with primary antibodies against PPARα (Proteintech, 66826-1-Ig), CYP4F3 (Proteintech, 19701-1-AP), and CYP4A10 (Abcam, ab3573) diluted 1:1000 in blocking buffer.

Techniques: Expressing, Western Blot, Flow Cytometry, Control