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polydatin (TargetMol)

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TargetMol polydatin

<t>Polydatin</t> inhibits HNRNPA1 lactylation and its biological activity in bladder cancer. A Binding free energy of ten candidate compounds docked to the HNRNPA1 active pocket. B Chemical structure of polydatin. C Docking visualization of polydatin within the active pocket of HNRNPA1. D IC 50 values of polydatin in the EJ-1 cell line, as determined by CCK8 assay. E EJ-1 cells were lysed and immunoprecipitated using an anti-HNRNPA1 antibody, followed by detection of HNRNPA1-K350 lactyl lysine. F Colony formation assays were performed to assess the proliferative capacity of EJ-1 cells following 24-h treatment with 30 μM polydatin or 20 mM NaLa. G-H Tumor gross images and tumor weights were assessed in nude mice after subcutaneous injection of EJ-1 cells. Tumors were harvested on Day 19 after subcutaneous implantation. Statistical analyses were performed with unpaired two-tailed Student’s t-test ( n = 5). I Tumor volume measurements throughout the experiment. J Immunohistochemical staining visualized PKM2 levels in xenograft tumors. Scale bars: 100 μm (left panel), 50 μm (right panel). K Schematic representation of the proposed mechanism of HNRNPA1 lactylation. * p < 0.05, ** p < 0.01

Polydatin, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polydatin/product/TargetMol
Average 93 stars, based on 5 article reviews

polydatin - by Bioz Stars, 2026-04

93/100 stars

Images

1) Product Images from "Lactate-driven lactylation of HNRNPA1 orchestrates PKM2 splicing and glycolytic reprogramming in bladder cancer"

Article Title: Lactate-driven lactylation of HNRNPA1 orchestrates PKM2 splicing and glycolytic reprogramming in bladder cancer

Journal: Journal of Experimental & Clinical Cancer Research : CR

doi: 10.1186/s13046-025-03591-5

Polydatin inhibits HNRNPA1 lactylation and its biological activity in bladder cancer. A Binding free energy of ten candidate compounds docked to the HNRNPA1 active pocket. B Chemical structure of polydatin. C Docking visualization of polydatin within the active pocket of HNRNPA1. D IC 50 values of polydatin in the EJ-1 cell line, as determined by CCK8 assay. E EJ-1 cells were lysed and immunoprecipitated using an anti-HNRNPA1 antibody, followed by detection of HNRNPA1-K350 lactyl lysine. F Colony formation assays were performed to assess the proliferative capacity of EJ-1 cells following 24-h treatment with 30 μM polydatin or 20 mM NaLa. G-H Tumor gross images and tumor weights were assessed in nude mice after subcutaneous injection of EJ-1 cells. Tumors were harvested on Day 19 after subcutaneous implantation. Statistical analyses were performed with unpaired two-tailed Student’s t-test ( n = 5). I Tumor volume measurements throughout the experiment. J Immunohistochemical staining visualized PKM2 levels in xenograft tumors. Scale bars: 100 μm (left panel), 50 μm (right panel). K Schematic representation of the proposed mechanism of HNRNPA1 lactylation. * p < 0.05, ** p < 0.01

Figure Legend Snippet: Polydatin inhibits HNRNPA1 lactylation and its biological activity in bladder cancer. A Binding free energy of ten candidate compounds docked to the HNRNPA1 active pocket. B Chemical structure of polydatin. C Docking visualization of polydatin within the active pocket of HNRNPA1. D IC 50 values of polydatin in the EJ-1 cell line, as determined by CCK8 assay. E EJ-1 cells were lysed and immunoprecipitated using an anti-HNRNPA1 antibody, followed by detection of HNRNPA1-K350 lactyl lysine. F Colony formation assays were performed to assess the proliferative capacity of EJ-1 cells following 24-h treatment with 30 μM polydatin or 20 mM NaLa. G-H Tumor gross images and tumor weights were assessed in nude mice after subcutaneous injection of EJ-1 cells. Tumors were harvested on Day 19 after subcutaneous implantation. Statistical analyses were performed with unpaired two-tailed Student’s t-test ( n = 5). I Tumor volume measurements throughout the experiment. J Immunohistochemical staining visualized PKM2 levels in xenograft tumors. Scale bars: 100 μm (left panel), 50 μm (right panel). K Schematic representation of the proposed mechanism of HNRNPA1 lactylation. * p < 0.05, ** p < 0.01

Techniques Used: Activity Assay, Binding Assay, CCK-8 Assay, Immunoprecipitation, Injection, Two Tailed Test, Immunohistochemical staining, Staining

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Core Target Network of Polydatin against LIRI. ( A ) Venn diagram of polydatin and LIRI-related targets. ( B ) PPI network of the common target genes. ( C ) Protein interaction network of core targets. ( D ) Top 10 Ranked Core Common Targets.

Journal: Journal of Inflammation Research

Article Title: Exploring the Mechanism of Therapeutic Effects of Polydatin in Lung Ischemia-Reperfusion Injury by Network Pharmacology and Experiment Validation

doi: 10.2147/JIR.S577340

Figure Lengend Snippet: Core Target Network of Polydatin against LIRI. ( A ) Venn diagram of polydatin and LIRI-related targets. ( B ) PPI network of the common target genes. ( C ) Protein interaction network of core targets. ( D ) Top 10 Ranked Core Common Targets.

Article Snippet: Polydatin (MedChemExpress, Shanghai, China) was first dissolved in dimethyl sulfoxide (DMSO) to yield a 10 mM stock solution.

Techniques:

GO and KEGG analysis. ( A ) GO functional analysis and ( B ) Enriched KEGG pathways associated with core targets of polydatin against LIRI.

Journal: Journal of Inflammation Research

Article Title: Exploring the Mechanism of Therapeutic Effects of Polydatin in Lung Ischemia-Reperfusion Injury by Network Pharmacology and Experiment Validation

doi: 10.2147/JIR.S577340

Figure Lengend Snippet: GO and KEGG analysis. ( A ) GO functional analysis and ( B ) Enriched KEGG pathways associated with core targets of polydatin against LIRI.

Article Snippet: Polydatin (MedChemExpress, Shanghai, China) was first dissolved in dimethyl sulfoxide (DMSO) to yield a 10 mM stock solution.

Techniques: Functional Assay

Polydatin protects A549 cells against H/R injury. ( A ) chemical structure of polydatin. ( B ) cell viability detected by CCK-8 assay after polydatin treatment. ( C ) CCK-8 detection of viability of H/R-induced cells following treatment with polydatin. Data are expressed as the mean ± SD (n=3). *** p < 0.001, **** p < 0.0001, ns: not statistically significant.

Journal: Journal of Inflammation Research

Article Title: Exploring the Mechanism of Therapeutic Effects of Polydatin in Lung Ischemia-Reperfusion Injury by Network Pharmacology and Experiment Validation

doi: 10.2147/JIR.S577340

Figure Lengend Snippet: Polydatin protects A549 cells against H/R injury. ( A ) chemical structure of polydatin. ( B ) cell viability detected by CCK-8 assay after polydatin treatment. ( C ) CCK-8 detection of viability of H/R-induced cells following treatment with polydatin. Data are expressed as the mean ± SD (n=3). *** p < 0.001, **** p < 0.0001, ns: not statistically significant.

Article Snippet: Polydatin (MedChemExpress, Shanghai, China) was first dissolved in dimethyl sulfoxide (DMSO) to yield a 10 mM stock solution.

Techniques: CCK-8 Assay

Effects of polydatin on apoptosis induced by H/R. ( A ) Fluorescence images showing TUNEL staining in A549 cells across experimental groups. Scale bar: 200 μm. ( B ) Statistical analysis of the proportion of TUNEL‑positive area. Data are expressed as the mean ± SD (n=3). ** p < 0.01, *** p < 0.001.

Journal: Journal of Inflammation Research

Article Title: Exploring the Mechanism of Therapeutic Effects of Polydatin in Lung Ischemia-Reperfusion Injury by Network Pharmacology and Experiment Validation

doi: 10.2147/JIR.S577340

Figure Lengend Snippet: Effects of polydatin on apoptosis induced by H/R. ( A ) Fluorescence images showing TUNEL staining in A549 cells across experimental groups. Scale bar: 200 μm. ( B ) Statistical analysis of the proportion of TUNEL‑positive area. Data are expressed as the mean ± SD (n=3). ** p < 0.01, *** p < 0.001.

Article Snippet: Polydatin (MedChemExpress, Shanghai, China) was first dissolved in dimethyl sulfoxide (DMSO) to yield a 10 mM stock solution.

Techniques: Fluorescence, TUNEL Assay, Staining

Effects of polydatin on H/R-induced levels of oxidative stress. ( A ) SOD, ( B ) MDA. Data are expressed as the mean ± SD (n=3). * p < 0.05, ** p < 0.01, ns: not statistically significant.

Journal: Journal of Inflammation Research

Article Title: Exploring the Mechanism of Therapeutic Effects of Polydatin in Lung Ischemia-Reperfusion Injury by Network Pharmacology and Experiment Validation

doi: 10.2147/JIR.S577340

Figure Lengend Snippet: Effects of polydatin on H/R-induced levels of oxidative stress. ( A ) SOD, ( B ) MDA. Data are expressed as the mean ± SD (n=3). * p < 0.05, ** p < 0.01, ns: not statistically significant.

Article Snippet: Polydatin (MedChemExpress, Shanghai, China) was first dissolved in dimethyl sulfoxide (DMSO) to yield a 10 mM stock solution.

Techniques:

Effects of polydatin on H/R-induced levels of ROS. ( A ) Representative fluorescence images illustrating intracellular ROS levels (scale bar: 200 µm). ( B ) Quantification of ROS-associated fluorescence intensity. Data are expressed as the mean ± SD (n=3). *** p < 0.001.

Journal: Journal of Inflammation Research

Article Title: Exploring the Mechanism of Therapeutic Effects of Polydatin in Lung Ischemia-Reperfusion Injury by Network Pharmacology and Experiment Validation

doi: 10.2147/JIR.S577340

Figure Lengend Snippet: Effects of polydatin on H/R-induced levels of ROS. ( A ) Representative fluorescence images illustrating intracellular ROS levels (scale bar: 200 µm). ( B ) Quantification of ROS-associated fluorescence intensity. Data are expressed as the mean ± SD (n=3). *** p < 0.001.

Article Snippet: Polydatin (MedChemExpress, Shanghai, China) was first dissolved in dimethyl sulfoxide (DMSO) to yield a 10 mM stock solution.

Techniques: Fluorescence

Effects of polydatin on H/R-induced levels of inflammatory response. ( A ) IL-6, ( B ) IL-1β, ( C ) TNF-α levels. Data are expressed as the mean ± SD (n=3). * p < 0.05, ** p < 0.01, **** p < 0.0001, ns: not statistically significant.

Journal: Journal of Inflammation Research

Article Title: Exploring the Mechanism of Therapeutic Effects of Polydatin in Lung Ischemia-Reperfusion Injury by Network Pharmacology and Experiment Validation

doi: 10.2147/JIR.S577340

Figure Lengend Snippet: Effects of polydatin on H/R-induced levels of inflammatory response. ( A ) IL-6, ( B ) IL-1β, ( C ) TNF-α levels. Data are expressed as the mean ± SD (n=3). * p < 0.05, ** p < 0.01, **** p < 0.0001, ns: not statistically significant.

Article Snippet: Polydatin (MedChemExpress, Shanghai, China) was first dissolved in dimethyl sulfoxide (DMSO) to yield a 10 mM stock solution.

Techniques:

Effects of Polydatin on H/R-Induced mRNA Expression of AKT1, ALB, and TP53. ( A ) AKT1, ( B ) ALB, ( C ) TP53 levels. Data are expressed as the mean ± SD (n=3). * p < 0.05, *** p < 0.001, **** p < 0.0001, ns: not statistically significant.

Journal: Journal of Inflammation Research

Article Title: Exploring the Mechanism of Therapeutic Effects of Polydatin in Lung Ischemia-Reperfusion Injury by Network Pharmacology and Experiment Validation

doi: 10.2147/JIR.S577340

Figure Lengend Snippet: Effects of Polydatin on H/R-Induced mRNA Expression of AKT1, ALB, and TP53. ( A ) AKT1, ( B ) ALB, ( C ) TP53 levels. Data are expressed as the mean ± SD (n=3). * p < 0.05, *** p < 0.001, **** p < 0.0001, ns: not statistically significant.

Article Snippet: Polydatin (MedChemExpress, Shanghai, China) was first dissolved in dimethyl sulfoxide (DMSO) to yield a 10 mM stock solution.

Techniques: Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Lactate-driven lactylation of HNRNPA1 orchestrates PKM2 splicing and glycolytic reprogramming in bladder cancer

doi: 10.1186/s13046-025-03591-5

Figure Lengend Snippet: Polydatin inhibits HNRNPA1 lactylation and its biological activity in bladder cancer. A Binding free energy of ten candidate compounds docked to the HNRNPA1 active pocket. B Chemical structure of polydatin. C Docking visualization of polydatin within the active pocket of HNRNPA1. D IC 50 values of polydatin in the EJ-1 cell line, as determined by CCK8 assay. E EJ-1 cells were lysed and immunoprecipitated using an anti-HNRNPA1 antibody, followed by detection of HNRNPA1-K350 lactyl lysine. F Colony formation assays were performed to assess the proliferative capacity of EJ-1 cells following 24-h treatment with 30 μM polydatin or 20 mM NaLa. G-H Tumor gross images and tumor weights were assessed in nude mice after subcutaneous injection of EJ-1 cells. Tumors were harvested on Day 19 after subcutaneous implantation. Statistical analyses were performed with unpaired two-tailed Student’s t-test ( n = 5). I Tumor volume measurements throughout the experiment. J Immunohistochemical staining visualized PKM2 levels in xenograft tumors. Scale bars: 100 μm (left panel), 50 μm (right panel). K Schematic representation of the proposed mechanism of HNRNPA1 lactylation. * p < 0.05, ** p < 0.01

Article Snippet: Cells were treated with polydatin (27208–80-6, TargetMol, USA) for 24 h to examine cell proliferation.

Techniques: Activity Assay, Binding Assay, CCK-8 Assay, Immunoprecipitation, Injection, Two Tailed Test, Immunohistochemical staining, Staining

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1) Product Images from "Lactate-driven lactylation of HNRNPA1 orchestrates PKM2 splicing and glycolytic reprogramming in bladder cancer"

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