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Selleck Chemicals
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European Directorate for the Quality of Medicines and HealthCare
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ChromaDex
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ChromaDex
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Epitech Group Srl
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Beijing Solarbio Science
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Adooq Bioscience LLC
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Chengdu Herbpurify CO
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Cayman Chemical
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Epitech Group Srl
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Image Search Results
Journal: Cell Death & Disease
Article Title: The convergent roles of NF-κB and ER stress in sunitinib-mediated expression of pro-tumorigenic cytokines and refractory phenotype in renal cell carcinoma
doi: 10.1038/s41419-018-0388-1
Figure Lengend Snippet: a Sunitinib (Sun) induces degradation of IκBα and promotes nuclear translocation of Rel-A in 786-O and PNX0010 ccRCC cells. Cells were treated with 10 μM of sunitinib for 3 h. Cytoplasmic and nuclear extracts were subjected to western blot analysis using specific antibodies. b 786-O and PNX0010 cells were treated with 10 μM of sunitinib for 4 h. NF-κB (p65) DNA binding activity was examined using TransAM assay. Data are presented as the mean ± S.D. *** P < 0.0001 when compared with cells cultured in medium only (Med). c 786-O cells were treated as described above. NF-κB activity was examined by EMSA in nuclear extracts prepared from 786-O cells. Supershift analysis using p50 antibody was performed to confirm that the observed bands are specific for NF-κB. d Sunitinib augments expression of IL-6, IL-8 and TNF-α in ccRCC cells. 786-O cells were treated with 10 μM of sunitinib for 10 h. The levels of IL-6, IL-8 and TNF-α were evaluated by ELISA (enzyme-linked immunosorbent assay). Data are presented as the mean ± S.D. *** P < 0.0005 when compared with cells cultured in medium only
Article Snippet: ATF6 (sc-22799), Topo-1 (sc-32736),
Techniques: Translocation Assay, Western Blot, Binding Assay, Activity Assay, Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay