Journal: bioRxiv

Article Title: A novel viral protein translation mechanism reveals mitochondria as a target for antiviral drug development

doi: 10.1101/2020.10.19.344713

Figure Lengend Snippet: (A) Codon preferences in 12 RNA viruses and five DNA viruses were downloaded from the Codon Usage Database and the graph was drawn using GraphPad Prism. (B) The codon bias of Ad11 fiber protein was analysed with the Countcodon program in the Codon Usage Database. (C) Protein expression of Ad11 fiber was determined by Western blotting after transfection into HEK-293 cells after transfection with the plasmid containing Ad11 fiber native gene with or without MRP sequences. (D) Human A549 cells were infected with Ad11 virus for 1 hour at MOI=0.5 PFU/cell and further incubated with fresh medium containing 10 μM polydatin or lonidamine for 72 hours. Virus was titrated by TCID assay using HEK293 cells (*, p<0.05). (E) HEK293 cells were transfected with UTR-S constructs and treated with mitochondria-targeted drugs polydatin or lonidamine for 36 hours. Total protein was detected using Western Blot assay. (F) Schematic diagram of the proposed hypothesis regarding the viral hijacking of the mitochondrial translation machinery to support viral infection.

Article Snippet: For drug treatment assays, fresh medium containing DMSO (Sigma, USA), 10 μM polydatin (MCE,USA) or 10 μM lonidamine (MCE,USA) was used after transfection.

Techniques: Expressing, Western Blot, Transfection, Plasmid Preparation, Infection, Virus, Incubation, Construct

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Lactate-driven lactylation of HNRNPA1 orchestrates PKM2 splicing and glycolytic reprogramming in bladder cancer

doi: 10.1186/s13046-025-03591-5

Figure Lengend Snippet: Polydatin inhibits HNRNPA1 lactylation and its biological activity in bladder cancer. A Binding free energy of ten candidate compounds docked to the HNRNPA1 active pocket. B Chemical structure of polydatin. C Docking visualization of polydatin within the active pocket of HNRNPA1. D IC 50 values of polydatin in the EJ-1 cell line, as determined by CCK8 assay. E EJ-1 cells were lysed and immunoprecipitated using an anti-HNRNPA1 antibody, followed by detection of HNRNPA1-K350 lactyl lysine. F Colony formation assays were performed to assess the proliferative capacity of EJ-1 cells following 24-h treatment with 30 μM polydatin or 20 mM NaLa. G-H Tumor gross images and tumor weights were assessed in nude mice after subcutaneous injection of EJ-1 cells. Tumors were harvested on Day 19 after subcutaneous implantation. Statistical analyses were performed with unpaired two-tailed Student’s t-test ( n = 5). I Tumor volume measurements throughout the experiment. J Immunohistochemical staining visualized PKM2 levels in xenograft tumors. Scale bars: 100 μm (left panel), 50 μm (right panel). K Schematic representation of the proposed mechanism of HNRNPA1 lactylation. * p < 0.05, ** p < 0.01

Article Snippet: Cells were treated with polydatin (27208–80-6, TargetMol, USA) for 24 h to examine cell proliferation.

Techniques: Activity Assay, Binding Assay, CCK-8 Assay, Immunoprecipitation, Injection, Two Tailed Test, Immunohistochemical staining, Staining

Journal: Cell Death & Disease

Article Title: The convergent roles of NF-κB and ER stress in sunitinib-mediated expression of pro-tumorigenic cytokines and refractory phenotype in renal cell carcinoma

doi: 10.1038/s41419-018-0388-1

Figure Lengend Snippet: a Sunitinib (Sun) induces degradation of IκBα and promotes nuclear translocation of Rel-A in 786-O and PNX0010 ccRCC cells. Cells were treated with 10 μM of sunitinib for 3 h. Cytoplasmic and nuclear extracts were subjected to western blot analysis using specific antibodies. b 786-O and PNX0010 cells were treated with 10 μM of sunitinib for 4 h. NF-κB (p65) DNA binding activity was examined using TransAM assay. Data are presented as the mean ± S.D. *** P < 0.0001 when compared with cells cultured in medium only (Med). c 786-O cells were treated as described above. NF-κB activity was examined by EMSA in nuclear extracts prepared from 786-O cells. Supershift analysis using p50 antibody was performed to confirm that the observed bands are specific for NF-κB. d Sunitinib augments expression of IL-6, IL-8 and TNF-α in ccRCC cells. 786-O cells were treated with 10 μM of sunitinib for 10 h. The levels of IL-6, IL-8 and TNF-α were evaluated by ELISA (enzyme-linked immunosorbent assay). Data are presented as the mean ± S.D. *** P < 0.0005 when compared with cells cultured in medium only

Article Snippet: ATF6 (sc-22799), Topo-1 (sc-32736), IκBα (sc-203), β-actin (sc-130300) and HA-probe (sc-7392) antibodies were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA).

Techniques: Translocation Assay, Western Blot, Binding Assay, Activity Assay, Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Molecules

Article Title: The Neuroprotective Role of Polydatin: Neuropharmacological Mechanisms, Molecular Targets, Therapeutic Potentials, and Clinical Perspective

doi: 10.3390/molecules26195985

Figure Lengend Snippet: Polydatin, a glycosylated form of resveratrol.

Article Snippet: To modulate these mechanisms, polydatin was injected into adult male Sprague–Dawley rats in a single intraperitoneal dose.

Techniques:

Journal: Molecules

Article Title: The Neuroprotective Role of Polydatin: Neuropharmacological Mechanisms, Molecular Targets, Therapeutic Potentials, and Clinical Perspective

doi: 10.3390/molecules26195985

Figure Lengend Snippet: Neuropharmacological mechanisms of polydatin against different NDDs.

Article Snippet: To modulate these mechanisms, polydatin was injected into adult male Sprague–Dawley rats in a single intraperitoneal dose.

Techniques: In Vivo, In Vitro

Journal: Molecules

Article Title: The Neuroprotective Role of Polydatin: Neuropharmacological Mechanisms, Molecular Targets, Therapeutic Potentials, and Clinical Perspective

doi: 10.3390/molecules26195985

Figure Lengend Snippet: Polydatin employs several mediators to combat PD, AD, TBI/SCI, and stroke.

Article Snippet: To modulate these mechanisms, polydatin was injected into adult male Sprague–Dawley rats in a single intraperitoneal dose.

Techniques:

Journal: Molecules

Article Title: The Neuroprotective Role of Polydatin: Neuropharmacological Mechanisms, Molecular Targets, Therapeutic Potentials, and Clinical Perspective

doi: 10.3390/molecules26195985

Figure Lengend Snippet: Novel delivery systems of polydatin: Reduction in the pharmacokinetic limitations.

Article Snippet: To modulate these mechanisms, polydatin was injected into adult male Sprague–Dawley rats in a single intraperitoneal dose.

Techniques:

Journal: Molecules

Article Title: The Neuroprotective Role of Polydatin: Neuropharmacological Mechanisms, Molecular Targets, Therapeutic Potentials, and Clinical Perspective

doi: 10.3390/molecules26195985

Figure Lengend Snippet: Neuroprotective mechanisms of polydatin.

Article Snippet: To modulate these mechanisms, polydatin was injected into adult male Sprague–Dawley rats in a single intraperitoneal dose.

Techniques: