polydatin Search Results


polydatin  (MedChemExpress)
94
MedChemExpress polydatin
Effects of <t>polydatin</t> on drug sensitivity and ATG5 expression in AML cell lines (A) . The IC50 value of polydatin on KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were determined using the CCK-8 assay. (B) The cell viability of polydatin on KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were assessed using the CCK-8 assay. Compared with KASUMI-1, ** P < 0.01. (C) The ATG5 mRNA expression of KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were detected using the RT-qPCR assay. Compare with KASUMI-1 cell, * P < 0.05, ** P < 0.01.
Polydatin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/Polydatin/pmc11613146-35-73-78
Average 94 stars, based on 1 article reviews
polydatin - by Bioz Stars, 2026-09
94/100 stars
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polydatin  (Tokyo Chemical Industry)
86
Tokyo Chemical Industry polydatin
Effects of <t>polydatin</t> on drug sensitivity and ATG5 expression in AML cell lines (A) . The IC50 value of polydatin on KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were determined using the CCK-8 assay. (B) The cell viability of polydatin on KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were assessed using the CCK-8 assay. Compared with KASUMI-1, ** P < 0.01. (C) The ATG5 mRNA expression of KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were detected using the RT-qPCR assay. Compare with KASUMI-1 cell, * P < 0.05, ** P < 0.01.
Polydatin, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/polydatin/pmc12671244-583-43-44
Average 86 stars, based on 1 article reviews
polydatin - by Bioz Stars, 2026-09
86/100 stars
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iκbα  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology iκbα
a Sunitinib (Sun) induces degradation of <t>IκBα</t> and promotes nuclear translocation of Rel-A in 786-O and PNX0010 ccRCC cells. Cells were treated with 10 μM of sunitinib for 3 h. Cytoplasmic and nuclear extracts were subjected to western blot analysis using <t>specific</t> <t>antibodies.</t> b 786-O and PNX0010 cells were treated with 10 μM of sunitinib for 4 h. NF-κB (p65) DNA binding activity was examined using TransAM assay. Data are presented as the mean ± S.D. *** P < 0.0001 when compared with cells cultured in medium only (Med). c 786-O cells were treated as described above. NF-κB activity was examined by EMSA in nuclear extracts prepared from 786-O cells. Supershift analysis using p50 antibody was performed to confirm that the observed bands are specific for NF-κB. d Sunitinib augments expression of IL-6, IL-8 and TNF-α in ccRCC cells. 786-O cells were treated with 10 μM of sunitinib for 10 h. The levels of IL-6, IL-8 and TNF-α were evaluated by ELISA (enzyme-linked immunosorbent assay). Data are presented as the mean ± S.D. *** P < 0.0005 when compared with cells cultured in medium only
Iκbα, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/Polydatin/pmc05841329-151-4-15
Average 92 stars, based on 1 article reviews
iκbα - by Bioz Stars, 2026-09
92/100 stars
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polydatin  (Selleck Chemicals)
93
Selleck Chemicals polydatin
Identification of common binding residues with Go 6983 in a natural compounds library SBVS: EGCG, <t>polydatin,</t> and cyanidin inhibit MMP-1 expression in HaCaT cells. ( a ) Natural product library SBVS a search was conducted to identify common binding residues with Go 6983, leading to the selection of EGCG, polydatin, and cyanidin. Red writing, common binding residues. ( b ) Cells were treated with candidate; EGCG, polydatin and cyanidin for 24 h. Protein expression of MMP-1 was determined by western blotting analysis and quantified with Image J software. ### p < 0.001, ∗∗∗ p < 0.001 .
Polydatin, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/Polydatin/pmc11567019-47-2-6
Average 93 stars, based on 1 article reviews
polydatin - by Bioz Stars, 2026-09
93/100 stars
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europe equivalent  (European Directorate for the Quality of Medicines and HealthCare)
88
European Directorate for the Quality of Medicines and HealthCare europe equivalent
Identification of common binding residues with Go 6983 in a natural compounds library SBVS: EGCG, <t>polydatin,</t> and cyanidin inhibit MMP-1 expression in HaCaT cells. ( a ) Natural product library SBVS a search was conducted to identify common binding residues with Go 6983, leading to the selection of EGCG, polydatin, and cyanidin. Red writing, common binding residues. ( b ) Cells were treated with candidate; EGCG, polydatin and cyanidin for 24 h. Protein expression of MMP-1 was determined by western blotting analysis and quantified with Image J software. ### p < 0.001, ∗∗∗ p < 0.001 .
Europe Equivalent, supplied by European Directorate for the Quality of Medicines and HealthCare, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/Polydatin+CRS/10__3390_slash_molecules26102915-208-21-19
Average 88 stars, based on 1 article reviews
europe equivalent - by Bioz Stars, 2026-09
88/100 stars
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piceid polydatin  (ChromaDex)
90
ChromaDex piceid polydatin
Identification of common binding residues with Go 6983 in a natural compounds library SBVS: EGCG, <t>polydatin,</t> and cyanidin inhibit MMP-1 expression in HaCaT cells. ( a ) Natural product library SBVS a search was conducted to identify common binding residues with Go 6983, leading to the selection of EGCG, polydatin, and cyanidin. Red writing, common binding residues. ( b ) Cells were treated with candidate; EGCG, polydatin and cyanidin for 24 h. Protein expression of MMP-1 was determined by western blotting analysis and quantified with Image J software. ### p < 0.001, ∗∗∗ p < 0.001 .
Piceid Polydatin, supplied by ChromaDex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/Polydatin/pm18979462-44-30-34
Average 90 stars, based on 1 article reviews
piceid polydatin - by Bioz Stars, 2026-09
90/100 stars
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piceid standard  (ChromaDex)
92
ChromaDex piceid standard
Identification of common binding residues with Go 6983 in a natural compounds library SBVS: EGCG, <t>polydatin,</t> and cyanidin inhibit MMP-1 expression in HaCaT cells. ( a ) Natural product library SBVS a search was conducted to identify common binding residues with Go 6983, leading to the selection of EGCG, polydatin, and cyanidin. Red writing, common binding residues. ( b ) Cells were treated with candidate; EGCG, polydatin and cyanidin for 24 h. Protein expression of MMP-1 was determined by western blotting analysis and quantified with Image J software. ### p < 0.001, ∗∗∗ p < 0.001 .
Piceid Standard, supplied by ChromaDex, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/Polydatin/us07799357-186-1-6
Average 92 stars, based on 1 article reviews
piceid standard - by Bioz Stars, 2026-09
92/100 stars
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tablet containing m-pea plus polydatin (m-pea-pol; pelvilen forte, epitech spa)  (Epitech Group Srl)
90
Epitech Group Srl tablet containing m-pea plus polydatin (m-pea-pol; pelvilen forte, epitech spa)
Identification of common binding residues with Go 6983 in a natural compounds library SBVS: EGCG, <t>polydatin,</t> and cyanidin inhibit MMP-1 expression in HaCaT cells. ( a ) Natural product library SBVS a search was conducted to identify common binding residues with Go 6983, leading to the selection of EGCG, polydatin, and cyanidin. Red writing, common binding residues. ( b ) Cells were treated with candidate; EGCG, polydatin and cyanidin for 24 h. Protein expression of MMP-1 was determined by western blotting analysis and quantified with Image J software. ### p < 0.001, ∗∗∗ p < 0.001 .
Tablet Containing M Pea Plus Polydatin (M Pea Pol; Pelvilen Forte, Epitech Spa), supplied by Epitech Group Srl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/tablet+containing+m+pea+plus+polydatin++m+pea+pol++pelvilen+forte++epitech+spa+/pmc06885282-67-13-17
Average 90 stars, based on 1 article reviews
tablet containing m-pea plus polydatin (m-pea-pol; pelvilen forte, epitech spa) - by Bioz Stars, 2026-09
90/100 stars
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polydatin #a10741  (Adooq Bioscience LLC)
90
Adooq Bioscience LLC polydatin #a10741
Identification of common binding residues with Go 6983 in a natural compounds library SBVS: EGCG, <t>polydatin,</t> and cyanidin inhibit MMP-1 expression in HaCaT cells. ( a ) Natural product library SBVS a search was conducted to identify common binding residues with Go 6983, leading to the selection of EGCG, polydatin, and cyanidin. Red writing, common binding residues. ( b ) Cells were treated with candidate; EGCG, polydatin and cyanidin for 24 h. Protein expression of MMP-1 was determined by western blotting analysis and quantified with Image J software. ### p < 0.001, ∗∗∗ p < 0.001 .
Polydatin #A10741, supplied by Adooq Bioscience LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/polydatin++a10741/pm37515179-67-23-25
Average 90 stars, based on 1 article reviews
polydatin #a10741 - by Bioz Stars, 2026-09
90/100 stars
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polydatin chengdu herbpurify, cat#h-012-171216  (Chengdu Herbpurify CO)
90
Chengdu Herbpurify CO polydatin chengdu herbpurify, cat#h-012-171216
Identification of common binding residues with Go 6983 in a natural compounds library SBVS: EGCG, <t>polydatin,</t> and cyanidin inhibit MMP-1 expression in HaCaT cells. ( a ) Natural product library SBVS a search was conducted to identify common binding residues with Go 6983, leading to the selection of EGCG, polydatin, and cyanidin. Red writing, common binding residues. ( b ) Cells were treated with candidate; EGCG, polydatin and cyanidin for 24 h. Protein expression of MMP-1 was determined by western blotting analysis and quantified with Image J software. ### p < 0.001, ∗∗∗ p < 0.001 .
Polydatin Chengdu Herbpurify, Cat#H 012 171216, supplied by Chengdu Herbpurify CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/polydatin+Chengdu+Herbpurify++cat+H+012+171216/pmc10591627-58-11-13
Average 90 stars, based on 1 article reviews
polydatin chengdu herbpurify, cat#h-012-171216 - by Bioz Stars, 2026-09
90/100 stars
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co-micronized form pea/pd 200 mg/20  (Epitech Group Srl)
90
Epitech Group Srl co-micronized form pea/pd 200 mg/20
Identification of common binding residues with Go 6983 in a natural compounds library SBVS: EGCG, <t>polydatin,</t> and cyanidin inhibit MMP-1 expression in HaCaT cells. ( a ) Natural product library SBVS a search was conducted to identify common binding residues with Go 6983, leading to the selection of EGCG, polydatin, and cyanidin. Red writing, common binding residues. ( b ) Cells were treated with candidate; EGCG, polydatin and cyanidin for 24 h. Protein expression of MMP-1 was determined by western blotting analysis and quantified with Image J software. ### p < 0.001, ∗∗∗ p < 0.001 .
Co Micronized Form Pea/Pd 200 Mg/20, supplied by Epitech Group Srl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/co+micronised+form+palmithoylethanolamide+polydatin+200+mg+20/pm38479039-64-18-14
Average 90 stars, based on 1 article reviews
co-micronized form pea/pd 200 mg/20 - by Bioz Stars, 2026-09
90/100 stars
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polydatin  (Extrasynthese SA)
90
Extrasynthese SA polydatin
Composition and antioxidant activity of the Sorbus aucuparia L. ethanolic extract (the results are expressed as means ± standard deviations of three experiments).
Polydatin, supplied by Extrasynthese SA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydatin/polydatin/pmc08270275-219-2-19
Average 90 stars, based on 1 article reviews
polydatin - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


Effects of polydatin on drug sensitivity and ATG5 expression in AML cell lines (A) . The IC50 value of polydatin on KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were determined using the CCK-8 assay. (B) The cell viability of polydatin on KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were assessed using the CCK-8 assay. Compared with KASUMI-1, ** P < 0.01. (C) The ATG5 mRNA expression of KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were detected using the RT-qPCR assay. Compare with KASUMI-1 cell, * P < 0.05, ** P < 0.01.

Journal: Frontiers in Pharmacology

Article Title: Targeting autophagy: polydatin’s role in inducing cell death in AML

doi: 10.3389/fphar.2024.1470217

Figure Lengend Snippet: Effects of polydatin on drug sensitivity and ATG5 expression in AML cell lines (A) . The IC50 value of polydatin on KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were determined using the CCK-8 assay. (B) The cell viability of polydatin on KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were assessed using the CCK-8 assay. Compared with KASUMI-1, ** P < 0.01. (C) The ATG5 mRNA expression of KG-1, HL-60, C1498, KASUMI-1 and KASUMI-6 cells were detected using the RT-qPCR assay. Compare with KASUMI-1 cell, * P < 0.05, ** P < 0.01.

Article Snippet: Mouse hemoglobin (Hb) ELISA kit (No. LE-M1587) was purchased from Hefei Lyle Biotechnology Co., Ltd., China, and mouse erythropoietin (Epo) ELISA kit (No.XG-E989530) was purchased from Shanghai Xige Biotechnology Co., Ltd., China. human B-cell lymphoma-2 (BCL2) antibody (No.12789-1-AP), human Bcl-2 Associated X protein (BAX) antibody (No. 50599-2-Ig), human Poly ADP-ribose polymerase (PARP) antibody (No.13371-1-AP), ATG5 Monoclonal antibody (No. 66744-1-Ig) and human Cleaved caspase3 antibody (No.19677-1-AP) were purchased from Wuhan Proteintech Biological Co., Ltd. Polydatin (No.HY-N0120A) was purchased from MCE Biological Co., Ltd.

Techniques: Expressing, CCK-8 Assay, Quantitative RT-PCR

Polydatin inhibits the proliferation of AML cells and the expression of inflammatory factors in vitro (A) . CCK-8 assay was used to detect the effect of different concentrations of polydatin on the proliferation of KASUMI-1 cells; (B) . EDU assay was used to detect the effect of different concentrations of polydatin on the proliferation of KASUMI-1 cell; (C) . Statistical analysis of EDU results; (D–F) ELISA was used to detect the expression levels of IL-6, TNF-α and IL-1β in different concentrations of polydatin. * P < 0.05; ** P < 0.01; * P < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Targeting autophagy: polydatin’s role in inducing cell death in AML

doi: 10.3389/fphar.2024.1470217

Figure Lengend Snippet: Polydatin inhibits the proliferation of AML cells and the expression of inflammatory factors in vitro (A) . CCK-8 assay was used to detect the effect of different concentrations of polydatin on the proliferation of KASUMI-1 cells; (B) . EDU assay was used to detect the effect of different concentrations of polydatin on the proliferation of KASUMI-1 cell; (C) . Statistical analysis of EDU results; (D–F) ELISA was used to detect the expression levels of IL-6, TNF-α and IL-1β in different concentrations of polydatin. * P < 0.05; ** P < 0.01; * P < 0.001.

Article Snippet: Mouse hemoglobin (Hb) ELISA kit (No. LE-M1587) was purchased from Hefei Lyle Biotechnology Co., Ltd., China, and mouse erythropoietin (Epo) ELISA kit (No.XG-E989530) was purchased from Shanghai Xige Biotechnology Co., Ltd., China. human B-cell lymphoma-2 (BCL2) antibody (No.12789-1-AP), human Bcl-2 Associated X protein (BAX) antibody (No. 50599-2-Ig), human Poly ADP-ribose polymerase (PARP) antibody (No.13371-1-AP), ATG5 Monoclonal antibody (No. 66744-1-Ig) and human Cleaved caspase3 antibody (No.19677-1-AP) were purchased from Wuhan Proteintech Biological Co., Ltd. Polydatin (No.HY-N0120A) was purchased from MCE Biological Co., Ltd.

Techniques: Expressing, In Vitro, CCK-8 Assay, EdU Assay, Enzyme-linked Immunosorbent Assay

Polydatin induces apoptosis of AML cells in vitro (A) . Flow cytometry was used to detect the effect of different concentrations of polydatin on the apoptosis of KASUMI-1 cells; (B) . Statistical analysis of flow cytometry apoptosis test results; (C) . Western blot was used to detect the effects of different concentrations of polydatin on apoptosis-related proteins in KASUMI-1 cells; *** P < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Targeting autophagy: polydatin’s role in inducing cell death in AML

doi: 10.3389/fphar.2024.1470217

Figure Lengend Snippet: Polydatin induces apoptosis of AML cells in vitro (A) . Flow cytometry was used to detect the effect of different concentrations of polydatin on the apoptosis of KASUMI-1 cells; (B) . Statistical analysis of flow cytometry apoptosis test results; (C) . Western blot was used to detect the effects of different concentrations of polydatin on apoptosis-related proteins in KASUMI-1 cells; *** P < 0.001.

Article Snippet: Mouse hemoglobin (Hb) ELISA kit (No. LE-M1587) was purchased from Hefei Lyle Biotechnology Co., Ltd., China, and mouse erythropoietin (Epo) ELISA kit (No.XG-E989530) was purchased from Shanghai Xige Biotechnology Co., Ltd., China. human B-cell lymphoma-2 (BCL2) antibody (No.12789-1-AP), human Bcl-2 Associated X protein (BAX) antibody (No. 50599-2-Ig), human Poly ADP-ribose polymerase (PARP) antibody (No.13371-1-AP), ATG5 Monoclonal antibody (No. 66744-1-Ig) and human Cleaved caspase3 antibody (No.19677-1-AP) were purchased from Wuhan Proteintech Biological Co., Ltd. Polydatin (No.HY-N0120A) was purchased from MCE Biological Co., Ltd.

Techniques: In Vitro, Flow Cytometry, Western Blot

Polydatin activates ATG5-mediated autophagy pathway in vitro (A) . Molecular docking diagram of polydatin and ATG5; (B) . Western blot was used to detected the effect of polydatin on the expression of autophagy-related proteins; (C) . Statistical analysis of Western blot results. (D) Western-Blot analysis the level of ATG5 ubiquitination in KASUMI-1 cells treated with 0, 10, 20, 40 μM Polydatin; (E) Schematic illustration of the molecular mechanism of polydatin inhibits ATG5 ubiquitination to promote autophagy * P < 0.05; ** P < 0.01; * P < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Targeting autophagy: polydatin’s role in inducing cell death in AML

doi: 10.3389/fphar.2024.1470217

Figure Lengend Snippet: Polydatin activates ATG5-mediated autophagy pathway in vitro (A) . Molecular docking diagram of polydatin and ATG5; (B) . Western blot was used to detected the effect of polydatin on the expression of autophagy-related proteins; (C) . Statistical analysis of Western blot results. (D) Western-Blot analysis the level of ATG5 ubiquitination in KASUMI-1 cells treated with 0, 10, 20, 40 μM Polydatin; (E) Schematic illustration of the molecular mechanism of polydatin inhibits ATG5 ubiquitination to promote autophagy * P < 0.05; ** P < 0.01; * P < 0.001.

Article Snippet: Mouse hemoglobin (Hb) ELISA kit (No. LE-M1587) was purchased from Hefei Lyle Biotechnology Co., Ltd., China, and mouse erythropoietin (Epo) ELISA kit (No.XG-E989530) was purchased from Shanghai Xige Biotechnology Co., Ltd., China. human B-cell lymphoma-2 (BCL2) antibody (No.12789-1-AP), human Bcl-2 Associated X protein (BAX) antibody (No. 50599-2-Ig), human Poly ADP-ribose polymerase (PARP) antibody (No.13371-1-AP), ATG5 Monoclonal antibody (No. 66744-1-Ig) and human Cleaved caspase3 antibody (No.19677-1-AP) were purchased from Wuhan Proteintech Biological Co., Ltd. Polydatin (No.HY-N0120A) was purchased from MCE Biological Co., Ltd.

Techniques: In Vitro, Western Blot, Expressing, Ubiquitin Proteomics

Polydatin exerts anti-AML tumor effect by regulating ATG5-mediated autophagy pathway (A) . EDU was used to detect the effect of polydatin combined with agent-82 on the proliferation of KASUMI-1 cells. (B) Statistical analysis of EDU results; (C) ELISA was used to detect the effect of polydatin combined with agent-82 on the expression of IL-6, IL-1β and TNF-α in KASUMI-1 cells. (D) Flow cytometry was used to detect the effect of polydatin combined with agent-82 on the apoptosis of KASUMI-1 cells; (E) Statistical analysis of flow cytometry apoptosis test results; (F) Western blot was used to detect the effect of polydatin combined with agent-82 on apoptotic proteins in KASUMI-1 cells; (G) Statistical analysis of apoptotic protein expression results. Compared with control group, * P < 0.05; ** P < 0.01; * P < 0.001; compared with polydatin group, # P < 0.05; ## P < 0.01; ### P < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Targeting autophagy: polydatin’s role in inducing cell death in AML

doi: 10.3389/fphar.2024.1470217

Figure Lengend Snippet: Polydatin exerts anti-AML tumor effect by regulating ATG5-mediated autophagy pathway (A) . EDU was used to detect the effect of polydatin combined with agent-82 on the proliferation of KASUMI-1 cells. (B) Statistical analysis of EDU results; (C) ELISA was used to detect the effect of polydatin combined with agent-82 on the expression of IL-6, IL-1β and TNF-α in KASUMI-1 cells. (D) Flow cytometry was used to detect the effect of polydatin combined with agent-82 on the apoptosis of KASUMI-1 cells; (E) Statistical analysis of flow cytometry apoptosis test results; (F) Western blot was used to detect the effect of polydatin combined with agent-82 on apoptotic proteins in KASUMI-1 cells; (G) Statistical analysis of apoptotic protein expression results. Compared with control group, * P < 0.05; ** P < 0.01; * P < 0.001; compared with polydatin group, # P < 0.05; ## P < 0.01; ### P < 0.001.

Article Snippet: Mouse hemoglobin (Hb) ELISA kit (No. LE-M1587) was purchased from Hefei Lyle Biotechnology Co., Ltd., China, and mouse erythropoietin (Epo) ELISA kit (No.XG-E989530) was purchased from Shanghai Xige Biotechnology Co., Ltd., China. human B-cell lymphoma-2 (BCL2) antibody (No.12789-1-AP), human Bcl-2 Associated X protein (BAX) antibody (No. 50599-2-Ig), human Poly ADP-ribose polymerase (PARP) antibody (No.13371-1-AP), ATG5 Monoclonal antibody (No. 66744-1-Ig) and human Cleaved caspase3 antibody (No.19677-1-AP) were purchased from Wuhan Proteintech Biological Co., Ltd. Polydatin (No.HY-N0120A) was purchased from MCE Biological Co., Ltd.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Western Blot, Control

ATG5 inhibitor reversed polydatin-induced autophagy in AML cells. (A) Western blot was used to detect the effect of different concentrations of polydatin on autophagy protein in KASUMI-1 cells; (B – E) Statistical analysis of the results of western blot detection of the effect of polydatin on the expression of autophagy protein. Compared with control group, * P < 0.05; ** P < 0.01; * P < 0.001; compared with polydatin group, # P < 0.05; ## P < 0.01; ### P < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Targeting autophagy: polydatin’s role in inducing cell death in AML

doi: 10.3389/fphar.2024.1470217

Figure Lengend Snippet: ATG5 inhibitor reversed polydatin-induced autophagy in AML cells. (A) Western blot was used to detect the effect of different concentrations of polydatin on autophagy protein in KASUMI-1 cells; (B – E) Statistical analysis of the results of western blot detection of the effect of polydatin on the expression of autophagy protein. Compared with control group, * P < 0.05; ** P < 0.01; * P < 0.001; compared with polydatin group, # P < 0.05; ## P < 0.01; ### P < 0.001.

Article Snippet: Mouse hemoglobin (Hb) ELISA kit (No. LE-M1587) was purchased from Hefei Lyle Biotechnology Co., Ltd., China, and mouse erythropoietin (Epo) ELISA kit (No.XG-E989530) was purchased from Shanghai Xige Biotechnology Co., Ltd., China. human B-cell lymphoma-2 (BCL2) antibody (No.12789-1-AP), human Bcl-2 Associated X protein (BAX) antibody (No. 50599-2-Ig), human Poly ADP-ribose polymerase (PARP) antibody (No.13371-1-AP), ATG5 Monoclonal antibody (No. 66744-1-Ig) and human Cleaved caspase3 antibody (No.19677-1-AP) were purchased from Wuhan Proteintech Biological Co., Ltd. Polydatin (No.HY-N0120A) was purchased from MCE Biological Co., Ltd.

Techniques: Western Blot, Expressing, Control

Polydatin inhibits the growth of AML tumor cells in vivo . (A) Tumor gross appearance; (B) Average tumor volume; (C) Average tumor weight. (D) The survival curve of leukemia-bearing mice calculated by Kaplan-Meier estimate. (E) Body weight changes of mice; (F) The level of apoptosis was detected by immunofluorescence; (G) Statistical analysis of immunofluorescence results. Compare with DMSO group, * P < 0.05; ** P < 0.01; * P < 0.001; (H) Immunohistochemical staining for Ki67, Cleaved caspase 3, LC3 in tumor tissues at different doses.

Journal: Frontiers in Pharmacology

Article Title: Targeting autophagy: polydatin’s role in inducing cell death in AML

doi: 10.3389/fphar.2024.1470217

Figure Lengend Snippet: Polydatin inhibits the growth of AML tumor cells in vivo . (A) Tumor gross appearance; (B) Average tumor volume; (C) Average tumor weight. (D) The survival curve of leukemia-bearing mice calculated by Kaplan-Meier estimate. (E) Body weight changes of mice; (F) The level of apoptosis was detected by immunofluorescence; (G) Statistical analysis of immunofluorescence results. Compare with DMSO group, * P < 0.05; ** P < 0.01; * P < 0.001; (H) Immunohistochemical staining for Ki67, Cleaved caspase 3, LC3 in tumor tissues at different doses.

Article Snippet: Mouse hemoglobin (Hb) ELISA kit (No. LE-M1587) was purchased from Hefei Lyle Biotechnology Co., Ltd., China, and mouse erythropoietin (Epo) ELISA kit (No.XG-E989530) was purchased from Shanghai Xige Biotechnology Co., Ltd., China. human B-cell lymphoma-2 (BCL2) antibody (No.12789-1-AP), human Bcl-2 Associated X protein (BAX) antibody (No. 50599-2-Ig), human Poly ADP-ribose polymerase (PARP) antibody (No.13371-1-AP), ATG5 Monoclonal antibody (No. 66744-1-Ig) and human Cleaved caspase3 antibody (No.19677-1-AP) were purchased from Wuhan Proteintech Biological Co., Ltd. Polydatin (No.HY-N0120A) was purchased from MCE Biological Co., Ltd.

Techniques: In Vivo, Immunofluorescence, Immunohistochemical staining, Staining

Polydatin improves AML tumor-related blood indicators in vivo (A) H&E staining for liver, spleen, kidney, lung and heart tissue sections from the DMSO or polydatin treated mice; (B) Analysis diagram of drug metabolism of polydatin in vivo ; (C) WBC counting of leukemia-bearing mice; (D, E) ELISA was used to detect the effect of polydatin on the expression of Hb and Epo in vivo . * P < 0.05; ** P < 0.01.

Journal: Frontiers in Pharmacology

Article Title: Targeting autophagy: polydatin’s role in inducing cell death in AML

doi: 10.3389/fphar.2024.1470217

Figure Lengend Snippet: Polydatin improves AML tumor-related blood indicators in vivo (A) H&E staining for liver, spleen, kidney, lung and heart tissue sections from the DMSO or polydatin treated mice; (B) Analysis diagram of drug metabolism of polydatin in vivo ; (C) WBC counting of leukemia-bearing mice; (D, E) ELISA was used to detect the effect of polydatin on the expression of Hb and Epo in vivo . * P < 0.05; ** P < 0.01.

Article Snippet: Mouse hemoglobin (Hb) ELISA kit (No. LE-M1587) was purchased from Hefei Lyle Biotechnology Co., Ltd., China, and mouse erythropoietin (Epo) ELISA kit (No.XG-E989530) was purchased from Shanghai Xige Biotechnology Co., Ltd., China. human B-cell lymphoma-2 (BCL2) antibody (No.12789-1-AP), human Bcl-2 Associated X protein (BAX) antibody (No. 50599-2-Ig), human Poly ADP-ribose polymerase (PARP) antibody (No.13371-1-AP), ATG5 Monoclonal antibody (No. 66744-1-Ig) and human Cleaved caspase3 antibody (No.19677-1-AP) were purchased from Wuhan Proteintech Biological Co., Ltd. Polydatin (No.HY-N0120A) was purchased from MCE Biological Co., Ltd.

Techniques: In Vivo, Staining, Enzyme-linked Immunosorbent Assay, Expressing

a Sunitinib (Sun) induces degradation of IκBα and promotes nuclear translocation of Rel-A in 786-O and PNX0010 ccRCC cells. Cells were treated with 10 μM of sunitinib for 3 h. Cytoplasmic and nuclear extracts were subjected to western blot analysis using specific antibodies. b 786-O and PNX0010 cells were treated with 10 μM of sunitinib for 4 h. NF-κB (p65) DNA binding activity was examined using TransAM assay. Data are presented as the mean ± S.D. *** P < 0.0001 when compared with cells cultured in medium only (Med). c 786-O cells were treated as described above. NF-κB activity was examined by EMSA in nuclear extracts prepared from 786-O cells. Supershift analysis using p50 antibody was performed to confirm that the observed bands are specific for NF-κB. d Sunitinib augments expression of IL-6, IL-8 and TNF-α in ccRCC cells. 786-O cells were treated with 10 μM of sunitinib for 10 h. The levels of IL-6, IL-8 and TNF-α were evaluated by ELISA (enzyme-linked immunosorbent assay). Data are presented as the mean ± S.D. *** P < 0.0005 when compared with cells cultured in medium only

Journal: Cell Death & Disease

Article Title: The convergent roles of NF-κB and ER stress in sunitinib-mediated expression of pro-tumorigenic cytokines and refractory phenotype in renal cell carcinoma

doi: 10.1038/s41419-018-0388-1

Figure Lengend Snippet: a Sunitinib (Sun) induces degradation of IκBα and promotes nuclear translocation of Rel-A in 786-O and PNX0010 ccRCC cells. Cells were treated with 10 μM of sunitinib for 3 h. Cytoplasmic and nuclear extracts were subjected to western blot analysis using specific antibodies. b 786-O and PNX0010 cells were treated with 10 μM of sunitinib for 4 h. NF-κB (p65) DNA binding activity was examined using TransAM assay. Data are presented as the mean ± S.D. *** P < 0.0001 when compared with cells cultured in medium only (Med). c 786-O cells were treated as described above. NF-κB activity was examined by EMSA in nuclear extracts prepared from 786-O cells. Supershift analysis using p50 antibody was performed to confirm that the observed bands are specific for NF-κB. d Sunitinib augments expression of IL-6, IL-8 and TNF-α in ccRCC cells. 786-O cells were treated with 10 μM of sunitinib for 10 h. The levels of IL-6, IL-8 and TNF-α were evaluated by ELISA (enzyme-linked immunosorbent assay). Data are presented as the mean ± S.D. *** P < 0.0005 when compared with cells cultured in medium only

Article Snippet: ATF6 (sc-22799), Topo-1 (sc-32736), IκBα (sc-203), β-actin (sc-130300) and HA-probe (sc-7392) antibodies were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA).

Techniques: Translocation Assay, Western Blot, Binding Assay, Activity Assay, Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay

Identification of common binding residues with Go 6983 in a natural compounds library SBVS: EGCG, polydatin, and cyanidin inhibit MMP-1 expression in HaCaT cells. ( a ) Natural product library SBVS a search was conducted to identify common binding residues with Go 6983, leading to the selection of EGCG, polydatin, and cyanidin. Red writing, common binding residues. ( b ) Cells were treated with candidate; EGCG, polydatin and cyanidin for 24 h. Protein expression of MMP-1 was determined by western blotting analysis and quantified with Image J software. ### p < 0.001, ∗∗∗ p < 0.001 .

Journal: Heliyon

Article Title: Structure-based virtual screening of natural compounds in preventing skin senescence: The role of epigallocatechin gallate in protein kinase C alpha-specific inhibition against UV-induced photoaging

doi: 10.1016/j.heliyon.2024.e39933

Figure Lengend Snippet: Identification of common binding residues with Go 6983 in a natural compounds library SBVS: EGCG, polydatin, and cyanidin inhibit MMP-1 expression in HaCaT cells. ( a ) Natural product library SBVS a search was conducted to identify common binding residues with Go 6983, leading to the selection of EGCG, polydatin, and cyanidin. Red writing, common binding residues. ( b ) Cells were treated with candidate; EGCG, polydatin and cyanidin for 24 h. Protein expression of MMP-1 was determined by western blotting analysis and quantified with Image J software. ### p < 0.001, ∗∗∗ p < 0.001 .

Article Snippet: EGCG and polydatin were obtained from Selleckchem (Houston, TX, USA).

Techniques: Binding Assay, Expressing, Selection, Western Blot, Software

Composition and antioxidant activity of the Sorbus aucuparia L. ethanolic extract (the results are expressed as means ± standard deviations of three experiments).

Journal: Molecules

Article Title: The Influence of Temperature, Storage Conditions, pH, and Ionic Strength on the Antioxidant Activity and Color Parameters of Rowan Berry Extracts

doi: 10.3390/molecules26133786

Figure Lengend Snippet: Composition and antioxidant activity of the Sorbus aucuparia L. ethanolic extract (the results are expressed as means ± standard deviations of three experiments).

Article Snippet: Procyanidin B1, procyanidin B2, polydatin, hyperoside, carotenoid standards, β -carotene, lycopene, lutein, zeaxanthin, and β -cryptoxanthin were purchased from Extrasynthese (Genay, France).

Techniques: Antioxidant Activity Assay

Polyphenols used as standards in HPLC analysis and their retention times.

Journal: Molecules

Article Title: The Influence of Temperature, Storage Conditions, pH, and Ionic Strength on the Antioxidant Activity and Color Parameters of Rowan Berry Extracts

doi: 10.3390/molecules26133786

Figure Lengend Snippet: Polyphenols used as standards in HPLC analysis and their retention times.

Article Snippet: Procyanidin B1, procyanidin B2, polydatin, hyperoside, carotenoid standards, β -carotene, lycopene, lutein, zeaxanthin, and β -cryptoxanthin were purchased from Extrasynthese (Genay, France).

Techniques: