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v4 10x genomics pn 1000691 primescript rt master mix takara bio rr036a xf cell mito stress test kit agilent technologies  (TaKaRa)


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    TaKaRa v4 10x genomics pn 1000691 primescript rt master mix takara bio rr036a xf cell mito stress test kit agilent technologies
    V4 10x Genomics Pn 1000691 Primescript Rt Master Mix Takara Bio Rr036a Xf Cell Mito Stress Test Kit Agilent Technologies, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 27259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pn/PrimeScript+RT+Master+Mix/pm42887499-260-80-88
    Average 99 stars, based on 27259 article reviews
    v4 10x genomics pn 1000691 primescript rt master mix takara bio rr036a xf cell mito stress test kit agilent technologies - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Dynamics and necessity of SIRT1 for maternal–zygotic transition
    Article Snippet: The quantity and quality of the isolated RNA were measured using a NanoDrop ND-2000 (NanoDrop Technologies) and analysed with an Agilent 2100 Bioanalyzer (Agilent Technologies). .. The isolated RNA was processed using the Takara Smarter Stranded Total RNA-seq Kit v2 Pico Input Mammalian (PN:634,413) according to the manufacturer's instructions. .. Libraries were sequenced on an Illumina NextSeq® 500 instrument using a 75 bp single-end configuration on a high-output flowcell.

    RNA Sequencing:

    Article Title: Dynamics and necessity of SIRT1 for maternal–zygotic transition
    Article Snippet: The quantity and quality of the isolated RNA were measured using a NanoDrop ND-2000 (NanoDrop Technologies) and analysed with an Agilent 2100 Bioanalyzer (Agilent Technologies). .. The isolated RNA was processed using the Takara Smarter Stranded Total RNA-seq Kit v2 Pico Input Mammalian (PN:634,413) according to the manufacturer's instructions. .. Libraries were sequenced on an Illumina NextSeq® 500 instrument using a 75 bp single-end configuration on a high-output flowcell.

    Cell Culture:

    Article Title: Cdc25A phosphatase is activated and mediates neuronal cell death by PUMA via pRB/E2F1 pathway in a model of Parkinson’s disease
    Article Snippet: NGF (cat no. N2513) and poly-d-lysine (cat no. P6407) were purchased from Sigma. .. Cell culture media Dulbecco’s modified Eagle’s medium (DMEM) (cat no. 31600-034), Neurobasal (cat no. 21103-049), B27, Pen Strep (cat no. 15140-122) Lipofectamine 2000 (cat no. PN 52887), Alexa Fluor (Cat no. A11008, A11003), horse serum (HS) (cat no. 26050088), and fetal bovine serum (FBS) (cat no.10082147) were purchased from Thermo Fisher Scientific. cDNA synthesis kit (cat no. 6110B) and real-time PCR kit (cat no. PN 4367659) were purchased from TAKARA. .. ECL reagent (cat no. RPN2232) and PVDF membrane (cat no. 10600023) were purchased from GE Healthcare.

    Article Title: Organotypic vascularization improves maturation of human stem cell-derived islets and restores normoglycemia in diabetic mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Glucose Sigma G7021 Sodium bicarbonate Sigma S6014 Bovine Serum Albumin (BSA) Proliant Biologicals 68700 GlutaMax Thermo Fisher 35050061 Recombinant human Activin A protein Stem Cell Technologies 78001 Recombinant human FGF2 protein Miltenyi Biotec 130-093-838 CHIR99021 Tocris 4423 L-Ascorbic acid Sigma A4544 Recombinant human KGF protein PeproTech AF-100-19 ITS-X Thermo Fisher 51500056 LDN193189 Reprocell 04-0074 TPPB Tocris 5343 Retinoic acid Sigma R2625 SANT1 Tocris 1974 Heparin Sigma H3149 ALK5i II Tocris 3742 XXI Stem Cell Technologies 73954 T3 Sigma T2877 latrunculin A Tocris 3973 MEM nonessential amino acids Thermo Fisher 11-140-050 ZnSO4 Sigma 1.08883 Trace Elements A Corning 25-021-CI Trace Elements B Corning 25-022-CI KnockOut DMEM/F12 medium Thermo Fisher 12660012 KnockOut Serum Replacement Thermo Fisher 10828028 2-Mercaptoethanol Sigma M3148 DMEM/F12 medium Thermo Fisher 11330032 Neurobasal medium Thermo Fisher 21103049 B27 supplement Thermo Fisher 12587010 N2 supplement Thermo Fisher 17502048 Recombinant human BMP4 protein R&D 314-BP Recombinant human VEGF-A protein PeproTech 100-20 Recombinant human NOGGIN protein PeproTech 120-10C Forskolin Sigma F3917 StemPro-34 SFM medium Thermo Fisher 10639011 Fetal Bovine Serum (FBS) Thermo Fisher 26140079 Collagen I solution Advanced Biomatrix 5005 Optimal Cutting Temperature (OCT) Thermo Fisher 23-730-571 Triton X-100 Sigma T8787 DAPI Thermo Fisher D3571 Tween 20 Sigma P7949 Dichloromethane (DCM) Sigma 270997 Glutaraldehyde Sigma G5882 Thapsigargin Cayman Chemical Company 10522 Lenvatinib MCE HY-10981 Fixation and Permeabilization Solution BD 554722 FastStart Universal SYBR Green Master BD 4913850001 Krebs buffer Thermo Fisher J67795.AP CalbryteTM 590 AM AAT Bioquest 20700 (Continued on next page) ll OPEN ACCESSArticle Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Streptozotocin (STZ) R&D 1621 Lysine fixable Texas Red-Dextran Thermo Fisher D1864 Propidium iodide (PI) Stem Cell Technologies 75002 Anti-Adherence Rinsing Solution Stem Cell Technologies 07010 Type IV collagenase Worthington Biochemical LS004189 TSQ (N-(6-Methoxy-8-quinolyl)-p-toluene sulfonamide) Sigma SML2656 Critical commercial assays Human C-peptide ELISA Kit Mercodia 10-1136-01 Human INSULIN ELISA Kit Mercodia 10-1113-01 Ultrasensitive Human C-peptide ELISA kit Mercodia 10-1141-01 Chromium Next GEM Single Cell 3’ Kit v3.1 10x Genomics PN-1000268 Chromium GEM-X Single Cell 3’ Kit v4 10x Genomics PN-1000691 Primescript RT Master Mix Takara Bio RR036A XF CELL MITO STRESS TEST Kit Agilent Technologies 103015-100 Deposited data scRNA-seq data This paper GEO: GSE309003 Human fetal endothelial cell atlas scRNA-seq data Cao et al.89 https://www.science.org/doi/10.1126/ science.aba7721 Human adult endothelial cell atlas scRNA-seq data Tabula Sapiens Consortium90 https://www.science.org/doi/10.1126/ science.abl4896 Human pancreatic islet and acinar endothelial cell scRNA-seq data Craig-Schapiro et al.30 GEO: GSE221237 Primary pancreatic islet endothelial cell scRNA-seq data Yang et al.44 GEO: GSE247806 HUVEC culture scRNA-seq data Jun et al.24 GEO: GSE276815 iPSC-derived endothelial cell culture scRNA-seq data Yang et al.44 GEO: GSE247807 Islet organoid–endothelial cell co-culture scRNA-seq data Yang et al.44 GEO: GSE247807 Assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 BMP4-treated assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 Vascularized macrophage-islet organoid scRNA-seq data Yang et al.44 GEO: GSE247807 Pancreatic islet organoid control scRNA-seq data Yang et al.44 GEO: GSE247807 Human pediatric pancreatic islet scRNA-seq data Zhu et al.62 GEO: GSE202491 Human adult pancreatic islet scRNA-seq data Yang et al.44 GEO: GSE247806 Human adult pancreatic β-cell scRNA-seq data Xin et al.91 GEO: GSE114297 Human adult pancreatic islet scRNA-seq data (HPAP) Human Pancreas Analysis Program (HPAP)92 GEO: GSE148073 Human fetal pancreatic endocrine scRNA-seq data Yu et al.61 OMIX236 Experimental models: Cell lines WTC11 The Coriell Institute GM25256 WTC11-mTagRFP The Coriell Institute AICS-0054 H1-INS-GFP Generous gift from Dr. Francis Lynn Novakovsky et al.41 Experimental models: Organisms/strains Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory JAX strain #005557 Oligonucleotides Primers for qPCR, see Table S1 This paper N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ (Continued on next page) ll OPEN ACCESS Article e3 Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 ..

    Modification:

    Article Title: Cdc25A phosphatase is activated and mediates neuronal cell death by PUMA via pRB/E2F1 pathway in a model of Parkinson’s disease
    Article Snippet: NGF (cat no. N2513) and poly-d-lysine (cat no. P6407) were purchased from Sigma. .. Cell culture media Dulbecco’s modified Eagle’s medium (DMEM) (cat no. 31600-034), Neurobasal (cat no. 21103-049), B27, Pen Strep (cat no. 15140-122) Lipofectamine 2000 (cat no. PN 52887), Alexa Fluor (Cat no. A11008, A11003), horse serum (HS) (cat no. 26050088), and fetal bovine serum (FBS) (cat no.10082147) were purchased from Thermo Fisher Scientific. cDNA synthesis kit (cat no. 6110B) and real-time PCR kit (cat no. PN 4367659) were purchased from TAKARA. .. ECL reagent (cat no. RPN2232) and PVDF membrane (cat no. 10600023) were purchased from GE Healthcare.

    cDNA Synthesis:

    Article Title: Cdc25A phosphatase is activated and mediates neuronal cell death by PUMA via pRB/E2F1 pathway in a model of Parkinson’s disease
    Article Snippet: NGF (cat no. N2513) and poly-d-lysine (cat no. P6407) were purchased from Sigma. .. Cell culture media Dulbecco’s modified Eagle’s medium (DMEM) (cat no. 31600-034), Neurobasal (cat no. 21103-049), B27, Pen Strep (cat no. 15140-122) Lipofectamine 2000 (cat no. PN 52887), Alexa Fluor (Cat no. A11008, A11003), horse serum (HS) (cat no. 26050088), and fetal bovine serum (FBS) (cat no.10082147) were purchased from Thermo Fisher Scientific. cDNA synthesis kit (cat no. 6110B) and real-time PCR kit (cat no. PN 4367659) were purchased from TAKARA. .. ECL reagent (cat no. RPN2232) and PVDF membrane (cat no. 10600023) were purchased from GE Healthcare.

    Real-time Polymerase Chain Reaction:

    Article Title: Cdc25A phosphatase is activated and mediates neuronal cell death by PUMA via pRB/E2F1 pathway in a model of Parkinson’s disease
    Article Snippet: NGF (cat no. N2513) and poly-d-lysine (cat no. P6407) were purchased from Sigma. .. Cell culture media Dulbecco’s modified Eagle’s medium (DMEM) (cat no. 31600-034), Neurobasal (cat no. 21103-049), B27, Pen Strep (cat no. 15140-122) Lipofectamine 2000 (cat no. PN 52887), Alexa Fluor (Cat no. A11008, A11003), horse serum (HS) (cat no. 26050088), and fetal bovine serum (FBS) (cat no.10082147) were purchased from Thermo Fisher Scientific. cDNA synthesis kit (cat no. 6110B) and real-time PCR kit (cat no. PN 4367659) were purchased from TAKARA. .. ECL reagent (cat no. RPN2232) and PVDF membrane (cat no. 10600023) were purchased from GE Healthcare.

    Article Title: Organotypic vascularization improves maturation of human stem cell-derived islets and restores normoglycemia in diabetic mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Glucose Sigma G7021 Sodium bicarbonate Sigma S6014 Bovine Serum Albumin (BSA) Proliant Biologicals 68700 GlutaMax Thermo Fisher 35050061 Recombinant human Activin A protein Stem Cell Technologies 78001 Recombinant human FGF2 protein Miltenyi Biotec 130-093-838 CHIR99021 Tocris 4423 L-Ascorbic acid Sigma A4544 Recombinant human KGF protein PeproTech AF-100-19 ITS-X Thermo Fisher 51500056 LDN193189 Reprocell 04-0074 TPPB Tocris 5343 Retinoic acid Sigma R2625 SANT1 Tocris 1974 Heparin Sigma H3149 ALK5i II Tocris 3742 XXI Stem Cell Technologies 73954 T3 Sigma T2877 latrunculin A Tocris 3973 MEM nonessential amino acids Thermo Fisher 11-140-050 ZnSO4 Sigma 1.08883 Trace Elements A Corning 25-021-CI Trace Elements B Corning 25-022-CI KnockOut DMEM/F12 medium Thermo Fisher 12660012 KnockOut Serum Replacement Thermo Fisher 10828028 2-Mercaptoethanol Sigma M3148 DMEM/F12 medium Thermo Fisher 11330032 Neurobasal medium Thermo Fisher 21103049 B27 supplement Thermo Fisher 12587010 N2 supplement Thermo Fisher 17502048 Recombinant human BMP4 protein R&D 314-BP Recombinant human VEGF-A protein PeproTech 100-20 Recombinant human NOGGIN protein PeproTech 120-10C Forskolin Sigma F3917 StemPro-34 SFM medium Thermo Fisher 10639011 Fetal Bovine Serum (FBS) Thermo Fisher 26140079 Collagen I solution Advanced Biomatrix 5005 Optimal Cutting Temperature (OCT) Thermo Fisher 23-730-571 Triton X-100 Sigma T8787 DAPI Thermo Fisher D3571 Tween 20 Sigma P7949 Dichloromethane (DCM) Sigma 270997 Glutaraldehyde Sigma G5882 Thapsigargin Cayman Chemical Company 10522 Lenvatinib MCE HY-10981 Fixation and Permeabilization Solution BD 554722 FastStart Universal SYBR Green Master BD 4913850001 Krebs buffer Thermo Fisher J67795.AP CalbryteTM 590 AM AAT Bioquest 20700 (Continued on next page) ll OPEN ACCESSArticle Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Streptozotocin (STZ) R&D 1621 Lysine fixable Texas Red-Dextran Thermo Fisher D1864 Propidium iodide (PI) Stem Cell Technologies 75002 Anti-Adherence Rinsing Solution Stem Cell Technologies 07010 Type IV collagenase Worthington Biochemical LS004189 TSQ (N-(6-Methoxy-8-quinolyl)-p-toluene sulfonamide) Sigma SML2656 Critical commercial assays Human C-peptide ELISA Kit Mercodia 10-1136-01 Human INSULIN ELISA Kit Mercodia 10-1113-01 Ultrasensitive Human C-peptide ELISA kit Mercodia 10-1141-01 Chromium Next GEM Single Cell 3’ Kit v3.1 10x Genomics PN-1000268 Chromium GEM-X Single Cell 3’ Kit v4 10x Genomics PN-1000691 Primescript RT Master Mix Takara Bio RR036A XF CELL MITO STRESS TEST Kit Agilent Technologies 103015-100 Deposited data scRNA-seq data This paper GEO: GSE309003 Human fetal endothelial cell atlas scRNA-seq data Cao et al.89 https://www.science.org/doi/10.1126/ science.aba7721 Human adult endothelial cell atlas scRNA-seq data Tabula Sapiens Consortium90 https://www.science.org/doi/10.1126/ science.abl4896 Human pancreatic islet and acinar endothelial cell scRNA-seq data Craig-Schapiro et al.30 GEO: GSE221237 Primary pancreatic islet endothelial cell scRNA-seq data Yang et al.44 GEO: GSE247806 HUVEC culture scRNA-seq data Jun et al.24 GEO: GSE276815 iPSC-derived endothelial cell culture scRNA-seq data Yang et al.44 GEO: GSE247807 Islet organoid–endothelial cell co-culture scRNA-seq data Yang et al.44 GEO: GSE247807 Assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 BMP4-treated assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 Vascularized macrophage-islet organoid scRNA-seq data Yang et al.44 GEO: GSE247807 Pancreatic islet organoid control scRNA-seq data Yang et al.44 GEO: GSE247807 Human pediatric pancreatic islet scRNA-seq data Zhu et al.62 GEO: GSE202491 Human adult pancreatic islet scRNA-seq data Yang et al.44 GEO: GSE247806 Human adult pancreatic β-cell scRNA-seq data Xin et al.91 GEO: GSE114297 Human adult pancreatic islet scRNA-seq data (HPAP) Human Pancreas Analysis Program (HPAP)92 GEO: GSE148073 Human fetal pancreatic endocrine scRNA-seq data Yu et al.61 OMIX236 Experimental models: Cell lines WTC11 The Coriell Institute GM25256 WTC11-mTagRFP The Coriell Institute AICS-0054 H1-INS-GFP Generous gift from Dr. Francis Lynn Novakovsky et al.41 Experimental models: Organisms/strains Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory JAX strain #005557 Oligonucleotides Primers for qPCR, see Table S1 This paper N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ (Continued on next page) ll OPEN ACCESS Article e3 Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 ..

    cDNA Library Assay:

    Article Title: Defining the cellular origin of seminoma by transcriptional and epigenetic mapping to the normal human germline.
    Article Snippet: .. The cDNA library was prepared with an SMART-Seq HT Plus Kit (PN R400749, Takara) according to the manufacturer’s instructions. cDNA was purified with AMPure XP beads, and 75-base pair reads were sequenced on the Illumina NextSeq 500 platform according to the manufacturer’s protocol. ..

    Article Title: Defining the cellular origin of seminoma by transcriptional and epigenetic mapping to the normal human germline
    Article Snippet: Briefly, total RNA was quantified using Qubit Fluorometric quantification, and RNA integrity was verified with a TapeStation (Agilent). .. RNA Integrity Number equivalent (RINe) above 8 was used for downstream libraries preparation. cDNA library was prepared using Clontech SMART-Seq HT Plus Kit (PN R400749) according to the manufacturer’s instructions. cDNA was purified with AMPure XP beads. .. 75-base pair reads were sequenced on an Illumina NextSeq 500 machine according to the manufacturer’s protocol.

    Purification:

    Article Title: Defining the cellular origin of seminoma by transcriptional and epigenetic mapping to the normal human germline.
    Article Snippet: .. The cDNA library was prepared with an SMART-Seq HT Plus Kit (PN R400749, Takara) according to the manufacturer’s instructions. cDNA was purified with AMPure XP beads, and 75-base pair reads were sequenced on the Illumina NextSeq 500 platform according to the manufacturer’s protocol. ..

    Article Title: Defining the cellular origin of seminoma by transcriptional and epigenetic mapping to the normal human germline
    Article Snippet: Briefly, total RNA was quantified using Qubit Fluorometric quantification, and RNA integrity was verified with a TapeStation (Agilent). .. RNA Integrity Number equivalent (RINe) above 8 was used for downstream libraries preparation. cDNA library was prepared using Clontech SMART-Seq HT Plus Kit (PN R400749) according to the manufacturer’s instructions. cDNA was purified with AMPure XP beads. .. 75-base pair reads were sequenced on an Illumina NextSeq 500 machine according to the manufacturer’s protocol.

    Stem Cell:

    Article Title: Organotypic vascularization improves maturation of human stem cell-derived islets and restores normoglycemia in diabetic mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Glucose Sigma G7021 Sodium bicarbonate Sigma S6014 Bovine Serum Albumin (BSA) Proliant Biologicals 68700 GlutaMax Thermo Fisher 35050061 Recombinant human Activin A protein Stem Cell Technologies 78001 Recombinant human FGF2 protein Miltenyi Biotec 130-093-838 CHIR99021 Tocris 4423 L-Ascorbic acid Sigma A4544 Recombinant human KGF protein PeproTech AF-100-19 ITS-X Thermo Fisher 51500056 LDN193189 Reprocell 04-0074 TPPB Tocris 5343 Retinoic acid Sigma R2625 SANT1 Tocris 1974 Heparin Sigma H3149 ALK5i II Tocris 3742 XXI Stem Cell Technologies 73954 T3 Sigma T2877 latrunculin A Tocris 3973 MEM nonessential amino acids Thermo Fisher 11-140-050 ZnSO4 Sigma 1.08883 Trace Elements A Corning 25-021-CI Trace Elements B Corning 25-022-CI KnockOut DMEM/F12 medium Thermo Fisher 12660012 KnockOut Serum Replacement Thermo Fisher 10828028 2-Mercaptoethanol Sigma M3148 DMEM/F12 medium Thermo Fisher 11330032 Neurobasal medium Thermo Fisher 21103049 B27 supplement Thermo Fisher 12587010 N2 supplement Thermo Fisher 17502048 Recombinant human BMP4 protein R&D 314-BP Recombinant human VEGF-A protein PeproTech 100-20 Recombinant human NOGGIN protein PeproTech 120-10C Forskolin Sigma F3917 StemPro-34 SFM medium Thermo Fisher 10639011 Fetal Bovine Serum (FBS) Thermo Fisher 26140079 Collagen I solution Advanced Biomatrix 5005 Optimal Cutting Temperature (OCT) Thermo Fisher 23-730-571 Triton X-100 Sigma T8787 DAPI Thermo Fisher D3571 Tween 20 Sigma P7949 Dichloromethane (DCM) Sigma 270997 Glutaraldehyde Sigma G5882 Thapsigargin Cayman Chemical Company 10522 Lenvatinib MCE HY-10981 Fixation and Permeabilization Solution BD 554722 FastStart Universal SYBR Green Master BD 4913850001 Krebs buffer Thermo Fisher J67795.AP CalbryteTM 590 AM AAT Bioquest 20700 (Continued on next page) ll OPEN ACCESSArticle Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Streptozotocin (STZ) R&D 1621 Lysine fixable Texas Red-Dextran Thermo Fisher D1864 Propidium iodide (PI) Stem Cell Technologies 75002 Anti-Adherence Rinsing Solution Stem Cell Technologies 07010 Type IV collagenase Worthington Biochemical LS004189 TSQ (N-(6-Methoxy-8-quinolyl)-p-toluene sulfonamide) Sigma SML2656 Critical commercial assays Human C-peptide ELISA Kit Mercodia 10-1136-01 Human INSULIN ELISA Kit Mercodia 10-1113-01 Ultrasensitive Human C-peptide ELISA kit Mercodia 10-1141-01 Chromium Next GEM Single Cell 3’ Kit v3.1 10x Genomics PN-1000268 Chromium GEM-X Single Cell 3’ Kit v4 10x Genomics PN-1000691 Primescript RT Master Mix Takara Bio RR036A XF CELL MITO STRESS TEST Kit Agilent Technologies 103015-100 Deposited data scRNA-seq data This paper GEO: GSE309003 Human fetal endothelial cell atlas scRNA-seq data Cao et al.89 https://www.science.org/doi/10.1126/ science.aba7721 Human adult endothelial cell atlas scRNA-seq data Tabula Sapiens Consortium90 https://www.science.org/doi/10.1126/ science.abl4896 Human pancreatic islet and acinar endothelial cell scRNA-seq data Craig-Schapiro et al.30 GEO: GSE221237 Primary pancreatic islet endothelial cell scRNA-seq data Yang et al.44 GEO: GSE247806 HUVEC culture scRNA-seq data Jun et al.24 GEO: GSE276815 iPSC-derived endothelial cell culture scRNA-seq data Yang et al.44 GEO: GSE247807 Islet organoid–endothelial cell co-culture scRNA-seq data Yang et al.44 GEO: GSE247807 Assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 BMP4-treated assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 Vascularized macrophage-islet organoid scRNA-seq data Yang et al.44 GEO: GSE247807 Pancreatic islet organoid control scRNA-seq data Yang et al.44 GEO: GSE247807 Human pediatric pancreatic islet scRNA-seq data Zhu et al.62 GEO: GSE202491 Human adult pancreatic islet scRNA-seq data Yang et al.44 GEO: GSE247806 Human adult pancreatic β-cell scRNA-seq data Xin et al.91 GEO: GSE114297 Human adult pancreatic islet scRNA-seq data (HPAP) Human Pancreas Analysis Program (HPAP)92 GEO: GSE148073 Human fetal pancreatic endocrine scRNA-seq data Yu et al.61 OMIX236 Experimental models: Cell lines WTC11 The Coriell Institute GM25256 WTC11-mTagRFP The Coriell Institute AICS-0054 H1-INS-GFP Generous gift from Dr. Francis Lynn Novakovsky et al.41 Experimental models: Organisms/strains Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory JAX strain #005557 Oligonucleotides Primers for qPCR, see Table S1 This paper N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ (Continued on next page) ll OPEN ACCESS Article e3 Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Organotypic vascularization improves maturation of human stem cell-derived islets and restores normoglycemia in diabetic mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Glucose Sigma G7021 Sodium bicarbonate Sigma S6014 Bovine Serum Albumin (BSA) Proliant Biologicals 68700 GlutaMax Thermo Fisher 35050061 Recombinant human Activin A protein Stem Cell Technologies 78001 Recombinant human FGF2 protein Miltenyi Biotec 130-093-838 CHIR99021 Tocris 4423 L-Ascorbic acid Sigma A4544 Recombinant human KGF protein PeproTech AF-100-19 ITS-X Thermo Fisher 51500056 LDN193189 Reprocell 04-0074 TPPB Tocris 5343 Retinoic acid Sigma R2625 SANT1 Tocris 1974 Heparin Sigma H3149 ALK5i II Tocris 3742 XXI Stem Cell Technologies 73954 T3 Sigma T2877 latrunculin A Tocris 3973 MEM nonessential amino acids Thermo Fisher 11-140-050 ZnSO4 Sigma 1.08883 Trace Elements A Corning 25-021-CI Trace Elements B Corning 25-022-CI KnockOut DMEM/F12 medium Thermo Fisher 12660012 KnockOut Serum Replacement Thermo Fisher 10828028 2-Mercaptoethanol Sigma M3148 DMEM/F12 medium Thermo Fisher 11330032 Neurobasal medium Thermo Fisher 21103049 B27 supplement Thermo Fisher 12587010 N2 supplement Thermo Fisher 17502048 Recombinant human BMP4 protein R&D 314-BP Recombinant human VEGF-A protein PeproTech 100-20 Recombinant human NOGGIN protein PeproTech 120-10C Forskolin Sigma F3917 StemPro-34 SFM medium Thermo Fisher 10639011 Fetal Bovine Serum (FBS) Thermo Fisher 26140079 Collagen I solution Advanced Biomatrix 5005 Optimal Cutting Temperature (OCT) Thermo Fisher 23-730-571 Triton X-100 Sigma T8787 DAPI Thermo Fisher D3571 Tween 20 Sigma P7949 Dichloromethane (DCM) Sigma 270997 Glutaraldehyde Sigma G5882 Thapsigargin Cayman Chemical Company 10522 Lenvatinib MCE HY-10981 Fixation and Permeabilization Solution BD 554722 FastStart Universal SYBR Green Master BD 4913850001 Krebs buffer Thermo Fisher J67795.AP CalbryteTM 590 AM AAT Bioquest 20700 (Continued on next page) ll OPEN ACCESSArticle Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Streptozotocin (STZ) R&D 1621 Lysine fixable Texas Red-Dextran Thermo Fisher D1864 Propidium iodide (PI) Stem Cell Technologies 75002 Anti-Adherence Rinsing Solution Stem Cell Technologies 07010 Type IV collagenase Worthington Biochemical LS004189 TSQ (N-(6-Methoxy-8-quinolyl)-p-toluene sulfonamide) Sigma SML2656 Critical commercial assays Human C-peptide ELISA Kit Mercodia 10-1136-01 Human INSULIN ELISA Kit Mercodia 10-1113-01 Ultrasensitive Human C-peptide ELISA kit Mercodia 10-1141-01 Chromium Next GEM Single Cell 3’ Kit v3.1 10x Genomics PN-1000268 Chromium GEM-X Single Cell 3’ Kit v4 10x Genomics PN-1000691 Primescript RT Master Mix Takara Bio RR036A XF CELL MITO STRESS TEST Kit Agilent Technologies 103015-100 Deposited data scRNA-seq data This paper GEO: GSE309003 Human fetal endothelial cell atlas scRNA-seq data Cao et al.89 https://www.science.org/doi/10.1126/ science.aba7721 Human adult endothelial cell atlas scRNA-seq data Tabula Sapiens Consortium90 https://www.science.org/doi/10.1126/ science.abl4896 Human pancreatic islet and acinar endothelial cell scRNA-seq data Craig-Schapiro et al.30 GEO: GSE221237 Primary pancreatic islet endothelial cell scRNA-seq data Yang et al.44 GEO: GSE247806 HUVEC culture scRNA-seq data Jun et al.24 GEO: GSE276815 iPSC-derived endothelial cell culture scRNA-seq data Yang et al.44 GEO: GSE247807 Islet organoid–endothelial cell co-culture scRNA-seq data Yang et al.44 GEO: GSE247807 Assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 BMP4-treated assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 Vascularized macrophage-islet organoid scRNA-seq data Yang et al.44 GEO: GSE247807 Pancreatic islet organoid control scRNA-seq data Yang et al.44 GEO: GSE247807 Human pediatric pancreatic islet scRNA-seq data Zhu et al.62 GEO: GSE202491 Human adult pancreatic islet scRNA-seq data Yang et al.44 GEO: GSE247806 Human adult pancreatic β-cell scRNA-seq data Xin et al.91 GEO: GSE114297 Human adult pancreatic islet scRNA-seq data (HPAP) Human Pancreas Analysis Program (HPAP)92 GEO: GSE148073 Human fetal pancreatic endocrine scRNA-seq data Yu et al.61 OMIX236 Experimental models: Cell lines WTC11 The Coriell Institute GM25256 WTC11-mTagRFP The Coriell Institute AICS-0054 H1-INS-GFP Generous gift from Dr. Francis Lynn Novakovsky et al.41 Experimental models: Organisms/strains Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory JAX strain #005557 Oligonucleotides Primers for qPCR, see Table S1 This paper N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ (Continued on next page) ll OPEN ACCESS Article e3 Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 ..

    Single Cell:

    Article Title: Organotypic vascularization improves maturation of human stem cell-derived islets and restores normoglycemia in diabetic mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Glucose Sigma G7021 Sodium bicarbonate Sigma S6014 Bovine Serum Albumin (BSA) Proliant Biologicals 68700 GlutaMax Thermo Fisher 35050061 Recombinant human Activin A protein Stem Cell Technologies 78001 Recombinant human FGF2 protein Miltenyi Biotec 130-093-838 CHIR99021 Tocris 4423 L-Ascorbic acid Sigma A4544 Recombinant human KGF protein PeproTech AF-100-19 ITS-X Thermo Fisher 51500056 LDN193189 Reprocell 04-0074 TPPB Tocris 5343 Retinoic acid Sigma R2625 SANT1 Tocris 1974 Heparin Sigma H3149 ALK5i II Tocris 3742 XXI Stem Cell Technologies 73954 T3 Sigma T2877 latrunculin A Tocris 3973 MEM nonessential amino acids Thermo Fisher 11-140-050 ZnSO4 Sigma 1.08883 Trace Elements A Corning 25-021-CI Trace Elements B Corning 25-022-CI KnockOut DMEM/F12 medium Thermo Fisher 12660012 KnockOut Serum Replacement Thermo Fisher 10828028 2-Mercaptoethanol Sigma M3148 DMEM/F12 medium Thermo Fisher 11330032 Neurobasal medium Thermo Fisher 21103049 B27 supplement Thermo Fisher 12587010 N2 supplement Thermo Fisher 17502048 Recombinant human BMP4 protein R&D 314-BP Recombinant human VEGF-A protein PeproTech 100-20 Recombinant human NOGGIN protein PeproTech 120-10C Forskolin Sigma F3917 StemPro-34 SFM medium Thermo Fisher 10639011 Fetal Bovine Serum (FBS) Thermo Fisher 26140079 Collagen I solution Advanced Biomatrix 5005 Optimal Cutting Temperature (OCT) Thermo Fisher 23-730-571 Triton X-100 Sigma T8787 DAPI Thermo Fisher D3571 Tween 20 Sigma P7949 Dichloromethane (DCM) Sigma 270997 Glutaraldehyde Sigma G5882 Thapsigargin Cayman Chemical Company 10522 Lenvatinib MCE HY-10981 Fixation and Permeabilization Solution BD 554722 FastStart Universal SYBR Green Master BD 4913850001 Krebs buffer Thermo Fisher J67795.AP CalbryteTM 590 AM AAT Bioquest 20700 (Continued on next page) ll OPEN ACCESSArticle Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Streptozotocin (STZ) R&D 1621 Lysine fixable Texas Red-Dextran Thermo Fisher D1864 Propidium iodide (PI) Stem Cell Technologies 75002 Anti-Adherence Rinsing Solution Stem Cell Technologies 07010 Type IV collagenase Worthington Biochemical LS004189 TSQ (N-(6-Methoxy-8-quinolyl)-p-toluene sulfonamide) Sigma SML2656 Critical commercial assays Human C-peptide ELISA Kit Mercodia 10-1136-01 Human INSULIN ELISA Kit Mercodia 10-1113-01 Ultrasensitive Human C-peptide ELISA kit Mercodia 10-1141-01 Chromium Next GEM Single Cell 3’ Kit v3.1 10x Genomics PN-1000268 Chromium GEM-X Single Cell 3’ Kit v4 10x Genomics PN-1000691 Primescript RT Master Mix Takara Bio RR036A XF CELL MITO STRESS TEST Kit Agilent Technologies 103015-100 Deposited data scRNA-seq data This paper GEO: GSE309003 Human fetal endothelial cell atlas scRNA-seq data Cao et al.89 https://www.science.org/doi/10.1126/ science.aba7721 Human adult endothelial cell atlas scRNA-seq data Tabula Sapiens Consortium90 https://www.science.org/doi/10.1126/ science.abl4896 Human pancreatic islet and acinar endothelial cell scRNA-seq data Craig-Schapiro et al.30 GEO: GSE221237 Primary pancreatic islet endothelial cell scRNA-seq data Yang et al.44 GEO: GSE247806 HUVEC culture scRNA-seq data Jun et al.24 GEO: GSE276815 iPSC-derived endothelial cell culture scRNA-seq data Yang et al.44 GEO: GSE247807 Islet organoid–endothelial cell co-culture scRNA-seq data Yang et al.44 GEO: GSE247807 Assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 BMP4-treated assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 Vascularized macrophage-islet organoid scRNA-seq data Yang et al.44 GEO: GSE247807 Pancreatic islet organoid control scRNA-seq data Yang et al.44 GEO: GSE247807 Human pediatric pancreatic islet scRNA-seq data Zhu et al.62 GEO: GSE202491 Human adult pancreatic islet scRNA-seq data Yang et al.44 GEO: GSE247806 Human adult pancreatic β-cell scRNA-seq data Xin et al.91 GEO: GSE114297 Human adult pancreatic islet scRNA-seq data (HPAP) Human Pancreas Analysis Program (HPAP)92 GEO: GSE148073 Human fetal pancreatic endocrine scRNA-seq data Yu et al.61 OMIX236 Experimental models: Cell lines WTC11 The Coriell Institute GM25256 WTC11-mTagRFP The Coriell Institute AICS-0054 H1-INS-GFP Generous gift from Dr. Francis Lynn Novakovsky et al.41 Experimental models: Organisms/strains Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory JAX strain #005557 Oligonucleotides Primers for qPCR, see Table S1 This paper N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ (Continued on next page) ll OPEN ACCESS Article e3 Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 ..

    Control:

    Article Title: Organotypic vascularization improves maturation of human stem cell-derived islets and restores normoglycemia in diabetic mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Glucose Sigma G7021 Sodium bicarbonate Sigma S6014 Bovine Serum Albumin (BSA) Proliant Biologicals 68700 GlutaMax Thermo Fisher 35050061 Recombinant human Activin A protein Stem Cell Technologies 78001 Recombinant human FGF2 protein Miltenyi Biotec 130-093-838 CHIR99021 Tocris 4423 L-Ascorbic acid Sigma A4544 Recombinant human KGF protein PeproTech AF-100-19 ITS-X Thermo Fisher 51500056 LDN193189 Reprocell 04-0074 TPPB Tocris 5343 Retinoic acid Sigma R2625 SANT1 Tocris 1974 Heparin Sigma H3149 ALK5i II Tocris 3742 XXI Stem Cell Technologies 73954 T3 Sigma T2877 latrunculin A Tocris 3973 MEM nonessential amino acids Thermo Fisher 11-140-050 ZnSO4 Sigma 1.08883 Trace Elements A Corning 25-021-CI Trace Elements B Corning 25-022-CI KnockOut DMEM/F12 medium Thermo Fisher 12660012 KnockOut Serum Replacement Thermo Fisher 10828028 2-Mercaptoethanol Sigma M3148 DMEM/F12 medium Thermo Fisher 11330032 Neurobasal medium Thermo Fisher 21103049 B27 supplement Thermo Fisher 12587010 N2 supplement Thermo Fisher 17502048 Recombinant human BMP4 protein R&D 314-BP Recombinant human VEGF-A protein PeproTech 100-20 Recombinant human NOGGIN protein PeproTech 120-10C Forskolin Sigma F3917 StemPro-34 SFM medium Thermo Fisher 10639011 Fetal Bovine Serum (FBS) Thermo Fisher 26140079 Collagen I solution Advanced Biomatrix 5005 Optimal Cutting Temperature (OCT) Thermo Fisher 23-730-571 Triton X-100 Sigma T8787 DAPI Thermo Fisher D3571 Tween 20 Sigma P7949 Dichloromethane (DCM) Sigma 270997 Glutaraldehyde Sigma G5882 Thapsigargin Cayman Chemical Company 10522 Lenvatinib MCE HY-10981 Fixation and Permeabilization Solution BD 554722 FastStart Universal SYBR Green Master BD 4913850001 Krebs buffer Thermo Fisher J67795.AP CalbryteTM 590 AM AAT Bioquest 20700 (Continued on next page) ll OPEN ACCESSArticle Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Streptozotocin (STZ) R&D 1621 Lysine fixable Texas Red-Dextran Thermo Fisher D1864 Propidium iodide (PI) Stem Cell Technologies 75002 Anti-Adherence Rinsing Solution Stem Cell Technologies 07010 Type IV collagenase Worthington Biochemical LS004189 TSQ (N-(6-Methoxy-8-quinolyl)-p-toluene sulfonamide) Sigma SML2656 Critical commercial assays Human C-peptide ELISA Kit Mercodia 10-1136-01 Human INSULIN ELISA Kit Mercodia 10-1113-01 Ultrasensitive Human C-peptide ELISA kit Mercodia 10-1141-01 Chromium Next GEM Single Cell 3’ Kit v3.1 10x Genomics PN-1000268 Chromium GEM-X Single Cell 3’ Kit v4 10x Genomics PN-1000691 Primescript RT Master Mix Takara Bio RR036A XF CELL MITO STRESS TEST Kit Agilent Technologies 103015-100 Deposited data scRNA-seq data This paper GEO: GSE309003 Human fetal endothelial cell atlas scRNA-seq data Cao et al.89 https://www.science.org/doi/10.1126/ science.aba7721 Human adult endothelial cell atlas scRNA-seq data Tabula Sapiens Consortium90 https://www.science.org/doi/10.1126/ science.abl4896 Human pancreatic islet and acinar endothelial cell scRNA-seq data Craig-Schapiro et al.30 GEO: GSE221237 Primary pancreatic islet endothelial cell scRNA-seq data Yang et al.44 GEO: GSE247806 HUVEC culture scRNA-seq data Jun et al.24 GEO: GSE276815 iPSC-derived endothelial cell culture scRNA-seq data Yang et al.44 GEO: GSE247807 Islet organoid–endothelial cell co-culture scRNA-seq data Yang et al.44 GEO: GSE247807 Assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 BMP4-treated assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 Vascularized macrophage-islet organoid scRNA-seq data Yang et al.44 GEO: GSE247807 Pancreatic islet organoid control scRNA-seq data Yang et al.44 GEO: GSE247807 Human pediatric pancreatic islet scRNA-seq data Zhu et al.62 GEO: GSE202491 Human adult pancreatic islet scRNA-seq data Yang et al.44 GEO: GSE247806 Human adult pancreatic β-cell scRNA-seq data Xin et al.91 GEO: GSE114297 Human adult pancreatic islet scRNA-seq data (HPAP) Human Pancreas Analysis Program (HPAP)92 GEO: GSE148073 Human fetal pancreatic endocrine scRNA-seq data Yu et al.61 OMIX236 Experimental models: Cell lines WTC11 The Coriell Institute GM25256 WTC11-mTagRFP The Coriell Institute AICS-0054 H1-INS-GFP Generous gift from Dr. Francis Lynn Novakovsky et al.41 Experimental models: Organisms/strains Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory JAX strain #005557 Oligonucleotides Primers for qPCR, see Table S1 This paper N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ (Continued on next page) ll OPEN ACCESS Article e3 Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 ..

    Analysis:

    Article Title: Organotypic vascularization improves maturation of human stem cell-derived islets and restores normoglycemia in diabetic mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Glucose Sigma G7021 Sodium bicarbonate Sigma S6014 Bovine Serum Albumin (BSA) Proliant Biologicals 68700 GlutaMax Thermo Fisher 35050061 Recombinant human Activin A protein Stem Cell Technologies 78001 Recombinant human FGF2 protein Miltenyi Biotec 130-093-838 CHIR99021 Tocris 4423 L-Ascorbic acid Sigma A4544 Recombinant human KGF protein PeproTech AF-100-19 ITS-X Thermo Fisher 51500056 LDN193189 Reprocell 04-0074 TPPB Tocris 5343 Retinoic acid Sigma R2625 SANT1 Tocris 1974 Heparin Sigma H3149 ALK5i II Tocris 3742 XXI Stem Cell Technologies 73954 T3 Sigma T2877 latrunculin A Tocris 3973 MEM nonessential amino acids Thermo Fisher 11-140-050 ZnSO4 Sigma 1.08883 Trace Elements A Corning 25-021-CI Trace Elements B Corning 25-022-CI KnockOut DMEM/F12 medium Thermo Fisher 12660012 KnockOut Serum Replacement Thermo Fisher 10828028 2-Mercaptoethanol Sigma M3148 DMEM/F12 medium Thermo Fisher 11330032 Neurobasal medium Thermo Fisher 21103049 B27 supplement Thermo Fisher 12587010 N2 supplement Thermo Fisher 17502048 Recombinant human BMP4 protein R&D 314-BP Recombinant human VEGF-A protein PeproTech 100-20 Recombinant human NOGGIN protein PeproTech 120-10C Forskolin Sigma F3917 StemPro-34 SFM medium Thermo Fisher 10639011 Fetal Bovine Serum (FBS) Thermo Fisher 26140079 Collagen I solution Advanced Biomatrix 5005 Optimal Cutting Temperature (OCT) Thermo Fisher 23-730-571 Triton X-100 Sigma T8787 DAPI Thermo Fisher D3571 Tween 20 Sigma P7949 Dichloromethane (DCM) Sigma 270997 Glutaraldehyde Sigma G5882 Thapsigargin Cayman Chemical Company 10522 Lenvatinib MCE HY-10981 Fixation and Permeabilization Solution BD 554722 FastStart Universal SYBR Green Master BD 4913850001 Krebs buffer Thermo Fisher J67795.AP CalbryteTM 590 AM AAT Bioquest 20700 (Continued on next page) ll OPEN ACCESSArticle Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Streptozotocin (STZ) R&D 1621 Lysine fixable Texas Red-Dextran Thermo Fisher D1864 Propidium iodide (PI) Stem Cell Technologies 75002 Anti-Adherence Rinsing Solution Stem Cell Technologies 07010 Type IV collagenase Worthington Biochemical LS004189 TSQ (N-(6-Methoxy-8-quinolyl)-p-toluene sulfonamide) Sigma SML2656 Critical commercial assays Human C-peptide ELISA Kit Mercodia 10-1136-01 Human INSULIN ELISA Kit Mercodia 10-1113-01 Ultrasensitive Human C-peptide ELISA kit Mercodia 10-1141-01 Chromium Next GEM Single Cell 3’ Kit v3.1 10x Genomics PN-1000268 Chromium GEM-X Single Cell 3’ Kit v4 10x Genomics PN-1000691 Primescript RT Master Mix Takara Bio RR036A XF CELL MITO STRESS TEST Kit Agilent Technologies 103015-100 Deposited data scRNA-seq data This paper GEO: GSE309003 Human fetal endothelial cell atlas scRNA-seq data Cao et al.89 https://www.science.org/doi/10.1126/ science.aba7721 Human adult endothelial cell atlas scRNA-seq data Tabula Sapiens Consortium90 https://www.science.org/doi/10.1126/ science.abl4896 Human pancreatic islet and acinar endothelial cell scRNA-seq data Craig-Schapiro et al.30 GEO: GSE221237 Primary pancreatic islet endothelial cell scRNA-seq data Yang et al.44 GEO: GSE247806 HUVEC culture scRNA-seq data Jun et al.24 GEO: GSE276815 iPSC-derived endothelial cell culture scRNA-seq data Yang et al.44 GEO: GSE247807 Islet organoid–endothelial cell co-culture scRNA-seq data Yang et al.44 GEO: GSE247807 Assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 BMP4-treated assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 Vascularized macrophage-islet organoid scRNA-seq data Yang et al.44 GEO: GSE247807 Pancreatic islet organoid control scRNA-seq data Yang et al.44 GEO: GSE247807 Human pediatric pancreatic islet scRNA-seq data Zhu et al.62 GEO: GSE202491 Human adult pancreatic islet scRNA-seq data Yang et al.44 GEO: GSE247806 Human adult pancreatic β-cell scRNA-seq data Xin et al.91 GEO: GSE114297 Human adult pancreatic islet scRNA-seq data (HPAP) Human Pancreas Analysis Program (HPAP)92 GEO: GSE148073 Human fetal pancreatic endocrine scRNA-seq data Yu et al.61 OMIX236 Experimental models: Cell lines WTC11 The Coriell Institute GM25256 WTC11-mTagRFP The Coriell Institute AICS-0054 H1-INS-GFP Generous gift from Dr. Francis Lynn Novakovsky et al.41 Experimental models: Organisms/strains Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory JAX strain #005557 Oligonucleotides Primers for qPCR, see Table S1 This paper N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ (Continued on next page) ll OPEN ACCESS Article e3 Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 ..

    Software:

    Article Title: Organotypic vascularization improves maturation of human stem cell-derived islets and restores normoglycemia in diabetic mice.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Glucose Sigma G7021 Sodium bicarbonate Sigma S6014 Bovine Serum Albumin (BSA) Proliant Biologicals 68700 GlutaMax Thermo Fisher 35050061 Recombinant human Activin A protein Stem Cell Technologies 78001 Recombinant human FGF2 protein Miltenyi Biotec 130-093-838 CHIR99021 Tocris 4423 L-Ascorbic acid Sigma A4544 Recombinant human KGF protein PeproTech AF-100-19 ITS-X Thermo Fisher 51500056 LDN193189 Reprocell 04-0074 TPPB Tocris 5343 Retinoic acid Sigma R2625 SANT1 Tocris 1974 Heparin Sigma H3149 ALK5i II Tocris 3742 XXI Stem Cell Technologies 73954 T3 Sigma T2877 latrunculin A Tocris 3973 MEM nonessential amino acids Thermo Fisher 11-140-050 ZnSO4 Sigma 1.08883 Trace Elements A Corning 25-021-CI Trace Elements B Corning 25-022-CI KnockOut DMEM/F12 medium Thermo Fisher 12660012 KnockOut Serum Replacement Thermo Fisher 10828028 2-Mercaptoethanol Sigma M3148 DMEM/F12 medium Thermo Fisher 11330032 Neurobasal medium Thermo Fisher 21103049 B27 supplement Thermo Fisher 12587010 N2 supplement Thermo Fisher 17502048 Recombinant human BMP4 protein R&D 314-BP Recombinant human VEGF-A protein PeproTech 100-20 Recombinant human NOGGIN protein PeproTech 120-10C Forskolin Sigma F3917 StemPro-34 SFM medium Thermo Fisher 10639011 Fetal Bovine Serum (FBS) Thermo Fisher 26140079 Collagen I solution Advanced Biomatrix 5005 Optimal Cutting Temperature (OCT) Thermo Fisher 23-730-571 Triton X-100 Sigma T8787 DAPI Thermo Fisher D3571 Tween 20 Sigma P7949 Dichloromethane (DCM) Sigma 270997 Glutaraldehyde Sigma G5882 Thapsigargin Cayman Chemical Company 10522 Lenvatinib MCE HY-10981 Fixation and Permeabilization Solution BD 554722 FastStart Universal SYBR Green Master BD 4913850001 Krebs buffer Thermo Fisher J67795.AP CalbryteTM 590 AM AAT Bioquest 20700 (Continued on next page) ll OPEN ACCESSArticle Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Streptozotocin (STZ) R&D 1621 Lysine fixable Texas Red-Dextran Thermo Fisher D1864 Propidium iodide (PI) Stem Cell Technologies 75002 Anti-Adherence Rinsing Solution Stem Cell Technologies 07010 Type IV collagenase Worthington Biochemical LS004189 TSQ (N-(6-Methoxy-8-quinolyl)-p-toluene sulfonamide) Sigma SML2656 Critical commercial assays Human C-peptide ELISA Kit Mercodia 10-1136-01 Human INSULIN ELISA Kit Mercodia 10-1113-01 Ultrasensitive Human C-peptide ELISA kit Mercodia 10-1141-01 Chromium Next GEM Single Cell 3’ Kit v3.1 10x Genomics PN-1000268 Chromium GEM-X Single Cell 3’ Kit v4 10x Genomics PN-1000691 Primescript RT Master Mix Takara Bio RR036A XF CELL MITO STRESS TEST Kit Agilent Technologies 103015-100 Deposited data scRNA-seq data This paper GEO: GSE309003 Human fetal endothelial cell atlas scRNA-seq data Cao et al.89 https://www.science.org/doi/10.1126/ science.aba7721 Human adult endothelial cell atlas scRNA-seq data Tabula Sapiens Consortium90 https://www.science.org/doi/10.1126/ science.abl4896 Human pancreatic islet and acinar endothelial cell scRNA-seq data Craig-Schapiro et al.30 GEO: GSE221237 Primary pancreatic islet endothelial cell scRNA-seq data Yang et al.44 GEO: GSE247806 HUVEC culture scRNA-seq data Jun et al.24 GEO: GSE276815 iPSC-derived endothelial cell culture scRNA-seq data Yang et al.44 GEO: GSE247807 Islet organoid–endothelial cell co-culture scRNA-seq data Yang et al.44 GEO: GSE247807 Assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 BMP4-treated assembled stem cell-derived islet scRNA-seq data Jun et al.24 GEO: GSE276815 Vascularized macrophage-islet organoid scRNA-seq data Yang et al.44 GEO: GSE247807 Pancreatic islet organoid control scRNA-seq data Yang et al.44 GEO: GSE247807 Human pediatric pancreatic islet scRNA-seq data Zhu et al.62 GEO: GSE202491 Human adult pancreatic islet scRNA-seq data Yang et al.44 GEO: GSE247806 Human adult pancreatic β-cell scRNA-seq data Xin et al.91 GEO: GSE114297 Human adult pancreatic islet scRNA-seq data (HPAP) Human Pancreas Analysis Program (HPAP)92 GEO: GSE148073 Human fetal pancreatic endocrine scRNA-seq data Yu et al.61 OMIX236 Experimental models: Cell lines WTC11 The Coriell Institute GM25256 WTC11-mTagRFP The Coriell Institute AICS-0054 H1-INS-GFP Generous gift from Dr. Francis Lynn Novakovsky et al.41 Experimental models: Organisms/strains Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) The Jackson Laboratory JAX strain #005557 Oligonucleotides Primers for qPCR, see Table S1 This paper N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ (Continued on next page) ll OPEN ACCESS Article e3 Cell Stem Cell 33, 1–17.e1–e9, November 5, 2026 ..



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