Review



phenylmethylsulfonyl fluoride  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    MedChemExpress phenylmethylsulfonyl fluoride
    Phenylmethylsulfonyl Fluoride, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsf/PMSF/pm42864360-513-11-14
    Average 97 stars, based on 370 article reviews
    phenylmethylsulfonyl fluoride - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Co-Immunoprecipitation Assay:

    Article Title: Mycobacterium tuberculosis-induced PCBP1 degradation drives macrophage ferroptosis to promote infection: a lung-macrophage-targeted RNAa nanotherapy.
    Article Snippet: .. PMSF (MCE, catalog: HY-B0496) and phosphatase inhibitors (Applygen, catalog: P1260) were added to the Co-IP buffer a few minutes before cell lysis to prevent protein degradation and dephosphorylation. .. In the Co-IP experiment, the anti-PCBP1 antibody (MBL, catalog: RN024P) and the anti-GPX4 antibody (antibodies-online, catalog: ABIN3015729) were both used at 5 μg/reaction.

    Lysis:

    Article Title: Mycobacterium tuberculosis-induced PCBP1 degradation drives macrophage ferroptosis to promote infection: a lung-macrophage-targeted RNAa nanotherapy.
    Article Snippet: .. PMSF (MCE, catalog: HY-B0496) and phosphatase inhibitors (Applygen, catalog: P1260) were added to the Co-IP buffer a few minutes before cell lysis to prevent protein degradation and dephosphorylation. .. In the Co-IP experiment, the anti-PCBP1 antibody (MBL, catalog: RN024P) and the anti-GPX4 antibody (antibodies-online, catalog: ABIN3015729) were both used at 5 μg/reaction.

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Article Title: PRMT5–mediated symmetric dimethylation of SHBs at Arg169 stabilizes SHBs and promotes angiogenesis and tumor growth
    Article Snippet: .. Cells were lysed in Western & IP lysis buffer (Beyotime Biotechnology, China) containing 20 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1% Triton X–100, sodium pyrophosphate, β–glycerophosphate, EDTA, Na 3 VO 4 , and leupeptin, supplemented with protease inhibitor cocktail and PMSF (MedChemExpress, USA), for 30 min at 4 °C. .. Protein concentrations were determined using a BCA Protein Assay Kit (Vazyme Biotech, China) according to the manufacturer's instructions.

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators.
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buf fered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incuba ted directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemEx press, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    De-Phosphorylation Assay:

    Article Title: Mycobacterium tuberculosis-induced PCBP1 degradation drives macrophage ferroptosis to promote infection: a lung-macrophage-targeted RNAa nanotherapy.
    Article Snippet: .. PMSF (MCE, catalog: HY-B0496) and phosphatase inhibitors (Applygen, catalog: P1260) were added to the Co-IP buffer a few minutes before cell lysis to prevent protein degradation and dephosphorylation. .. In the Co-IP experiment, the anti-PCBP1 antibody (MBL, catalog: RN024P) and the anti-GPX4 antibody (antibodies-online, catalog: ABIN3015729) were both used at 5 μg/reaction.

    Transfection:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Protease Inhibitor:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Article Title: PRMT5–mediated symmetric dimethylation of SHBs at Arg169 stabilizes SHBs and promotes angiogenesis and tumor growth
    Article Snippet: .. Cells were lysed in Western & IP lysis buffer (Beyotime Biotechnology, China) containing 20 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1% Triton X–100, sodium pyrophosphate, β–glycerophosphate, EDTA, Na 3 VO 4 , and leupeptin, supplemented with protease inhibitor cocktail and PMSF (MedChemExpress, USA), for 30 min at 4 °C. .. Protein concentrations were determined using a BCA Protein Assay Kit (Vazyme Biotech, China) according to the manufacturer's instructions.

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators.
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buf fered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incuba ted directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemEx press, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    Magnetic Beads:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators.
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buf fered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incuba ted directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemEx press, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    cDNA Synthesis:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Real-time Polymerase Chain Reaction:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    SYBR Green Assay:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    CCK-8 Assay:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Multiple Displacement Amplification:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Protein Extraction:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Bicinchoninic Acid Protein Assay:

    Article Title: Troxerutin mitigates ferroptosis-related neuroinflammation by regulating the microglial NOX4/Nrf2 axis in Parkinson's disease.
    Article Snippet: 22 Parkinson’s disease (PD) is characterized by progressive dopaminergic neurodegeneration and 23 chronic neuroinflammation.. Increasing evidence suggests that microglial ferroptosis plays a 24 critical role in the pathogenesis of PD.. Troxerutin (TRX), a natural flavonoid derivative with 25 potent antioxidant and anti-inflammatory activities, has shown neuroprotective potential; however, 26 its effects on microglial ferroptosis and the underlying mechanisms in PD remain unclear.

    Western Blot:

    Article Title: PRMT5–mediated symmetric dimethylation of SHBs at Arg169 stabilizes SHBs and promotes angiogenesis and tumor growth
    Article Snippet: .. Cells were lysed in Western & IP lysis buffer (Beyotime Biotechnology, China) containing 20 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1% Triton X–100, sodium pyrophosphate, β–glycerophosphate, EDTA, Na 3 VO 4 , and leupeptin, supplemented with protease inhibitor cocktail and PMSF (MedChemExpress, USA), for 30 min at 4 °C. .. Protein concentrations were determined using a BCA Protein Assay Kit (Vazyme Biotech, China) according to the manufacturer's instructions.

    Saline:

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators.
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buf fered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incuba ted directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemEx press, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    Immunoprecipitation:

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators.
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buf fered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incuba ted directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemEx press, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).

    Gentle:

    Article Title: Nipah virus matrix protein promotes NF-κB activation by targeting multiple signaling modulators.
    Article Snippet: .. At 24 hpt, cells were washed with cold phosphate-buf fered saline (PBS) and lysed in lysis buffer (P0013; Beyotime Biotechnology, Shanghai, China) containing protease inhibitor cocktail (HY-K0010; MedChemExpress, Shanghai, China) and PMSF (HY-B0496; MedChemExpress, Shanghai, China) on ice for 10 min. For immunoprecipitation (IP) experiments, the supernatant was then incuba ted directly with anti-Flag magnetic beads (HY-K0207; MedChemExpress, Shanghai, China) or anti-HA magnetic beads (HY-K0201; MedChemExpress, Shanghai, China) at 4°C overnight with gentle rotation. .. Alternatively, before cell lysis, Myc, V5, or IgG antibodies were incubated with Protein A/G magnetic beads (HY-K0202; MedChemEx press, Shanghai, China) in PBS at 4°C for at least 4 h. The antibody-conjugated beads were washed thrice with PBST (HY-K1022; MedChemExpress, Shanghai, China).



    Similar Products

    97
    MedChemExpress phenylmethylsulfonyl fluoride
    Phenylmethylsulfonyl Fluoride, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsf/PMSF/pm42864360-513-11-14
    Average 97 stars, based on 1 article reviews
    phenylmethylsulfonyl fluoride - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    94
    TargetMol pmsf
    Pmsf, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsf/PMSF/pmc13475575-333-15-16
    Average 94 stars, based on 1 article reviews
    pmsf - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    97
    MedChemExpress protease inhibitors pmsf
    Protease Inhibitors Pmsf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsf/PMSF/10__1038_slash_s41419___026___09318___4-57-14-17
    Average 97 stars, based on 1 article reviews
    protease inhibitors pmsf - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    96
    TargetMol phenylmethylsulfonyl fluoride pmsf
    Phenylmethylsulfonyl Fluoride Pmsf, supplied by TargetMol, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsf/Phosphatase+Inhibitor+Cocktail+I/pm42754128-98-14-17
    Average 96 stars, based on 1 article reviews
    phenylmethylsulfonyl fluoride pmsf - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    86
    Sangon Biotech pmsf
    Pmsf, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsf/pmsf/pmc13276161-160-27-29
    Average 86 stars, based on 1 article reviews
    pmsf - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    97
    MedChemExpress pmsf
    Pmsf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsf/PMSF/custom%40hy-b0496%4042519832
    Average 97 stars, based on 1 article reviews
    pmsf - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    97
    MedChemExpress hy b0496
    Hy B0496, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsf/PMSF/pm42519832-241-214-219
    Average 97 stars, based on 1 article reviews
    hy b0496 - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    86
    Servicebio Inc pmsf
    Pmsf, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsf/pmsf/pmc13276768-26-0-4
    Average 86 stars, based on 1 article reviews
    pmsf - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    97
    MedChemExpress phenylmethylsulfonyl fluoride pmsf
    Phenylmethylsulfonyl Fluoride Pmsf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmsf/PMSF/pmc13415939-102-26-34
    Average 97 stars, based on 1 article reviews
    phenylmethylsulfonyl fluoride pmsf - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    Image Search Results