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crat overexpression plx304 empty vector  (Addgene inc)


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    Addgene inc crat overexpression plx304 empty vector
    Crat Overexpression Plx304 Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plx304+empty+vector/pLX304+(Plasmid+%2325890)/pm41216931-58-2-8
    Average 95 stars, based on 297 article reviews
    crat overexpression plx304 empty vector - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma
    Article Snippet: .. A mixture of vsvg/viral envelope plasmid, dvpr/viral packaging vector, PLX304 empty vector (Addgene) or Nudt2 WT vector or PLX304-Luciferase-V5 vector were co-transfected into the 293T cells. ..

    Article Title: IGF2BP1 regulates the cargo of extracellular vesicles and promotes neuroblastoma metastasis
    Article Snippet: .. IGF2BP1 rescue M1 line was obtained using the same GFP-tagged IGF2BP1 construct and verified by western ( Supplementary Figure 3E ). . SEMA3A and SHMT2 OE/KD cell lines SEMA3A_pDONR221 and SHMT2_pLX304 plasmids were obtained from DNASU plasmid repository at Arizona State University. pDONR221_EGFP (RRID:Addgene_25899) and pLX304 empty vector (RRID:Addgene_25890) were obtained from Addgene. .. SEMA3A and EGFP were then cloned into pLX304 by LR recombination reaction (Gateway TM LR Clonase TM II Enzyme mix, Invitrogen) as per Invitrogen’s Gateway cloning protocol.

    Article Title: Dualism of FGF and TGF-β Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant
    Article Snippet: .. The pLX304 empty vector was used to produce control lentiviruses (Addgene plasmid #25890) ( ). ..

    Article Title: IGF2BP1 regulates the cargo of extracellular vesicles and promotes neuroblastoma metastasis
    Article Snippet: .. SEMA3A_pDONR221 and SHMT2_pLX304 plasmids were obtained from DNASU plasmid repository at Arizona State University. pDONR221_EGFP (RRID:Addgene_25899) and pLX304 empty vector (RRID:Addgene_25890) were obtained from Addgene. .. SEMA3A and EGFP were then cloned into pLX304 by LR recombination reaction (Gateway TM LR Clonase TM II Enzyme mix, Invitrogen) as per Invitrogen’s Gateway cloning protocol.

    Article Title: Dualism of FGF and TGF-β Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant
    Article Snippet: pLKO.1 human shETV1-5 , Dharmacon , TRCN0000013925. .. pLX304 empty vector , , Addgene plasmid #25890. .. pLX304-ETV1 , Broad Institute , CloneID: ccsbBroad304_00520.

    Construct:

    Article Title: IGF2BP1 regulates the cargo of extracellular vesicles and promotes neuroblastoma metastasis
    Article Snippet: .. IGF2BP1 rescue M1 line was obtained using the same GFP-tagged IGF2BP1 construct and verified by western ( Supplementary Figure 3E ). . SEMA3A and SHMT2 OE/KD cell lines SEMA3A_pDONR221 and SHMT2_pLX304 plasmids were obtained from DNASU plasmid repository at Arizona State University. pDONR221_EGFP (RRID:Addgene_25899) and pLX304 empty vector (RRID:Addgene_25890) were obtained from Addgene. .. SEMA3A and EGFP were then cloned into pLX304 by LR recombination reaction (Gateway TM LR Clonase TM II Enzyme mix, Invitrogen) as per Invitrogen’s Gateway cloning protocol.

    Western Blot:

    Article Title: IGF2BP1 regulates the cargo of extracellular vesicles and promotes neuroblastoma metastasis
    Article Snippet: .. IGF2BP1 rescue M1 line was obtained using the same GFP-tagged IGF2BP1 construct and verified by western ( Supplementary Figure 3E ). . SEMA3A and SHMT2 OE/KD cell lines SEMA3A_pDONR221 and SHMT2_pLX304 plasmids were obtained from DNASU plasmid repository at Arizona State University. pDONR221_EGFP (RRID:Addgene_25899) and pLX304 empty vector (RRID:Addgene_25890) were obtained from Addgene. .. SEMA3A and EGFP were then cloned into pLX304 by LR recombination reaction (Gateway TM LR Clonase TM II Enzyme mix, Invitrogen) as per Invitrogen’s Gateway cloning protocol.

    Control:

    Article Title: Dualism of FGF and TGF-β Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant
    Article Snippet: .. The pLX304 empty vector was used to produce control lentiviruses (Addgene plasmid #25890) ( ). ..

    Article Title: Yap1 Mediates Trametinib Resistance in Head and Neck Squamous Cell Carcinomas
    Article Snippet: After 48–72 hours, transfected cells were harvested, and lysates were analyzed using the Dual-Luciferase Reporter Assay Kit (Promega). .. pLenti-CRISPRv2 containing sgNT (single-guide nontargeting RNA), sgYAP1 #1–5 pLKO.1 containing scramble-shRNA (short hairpin RNA) control, shYAP1 #1–5 pLKO.1 containing YAP1 targeting sequences (see Supplementary Table S1), pLX304-empty vector (RRID:Addgene_25890), pLX304-human YAP1-WT, and pLX304human YAP1–5SA were generously provided by David Barbie (DanaFarber Cancer Institute, Boston,MA). .. HEK293T cells were transfected with 1 mg lentivirus-based expression vectors and packaging plasmids using X-tremeGENE 9 transfection reagent (Roche) according to the manufacturer’s instructions.

    shRNA:

    Article Title: Yap1 Mediates Trametinib Resistance in Head and Neck Squamous Cell Carcinomas
    Article Snippet: After 48–72 hours, transfected cells were harvested, and lysates were analyzed using the Dual-Luciferase Reporter Assay Kit (Promega). .. pLenti-CRISPRv2 containing sgNT (single-guide nontargeting RNA), sgYAP1 #1–5 pLKO.1 containing scramble-shRNA (short hairpin RNA) control, shYAP1 #1–5 pLKO.1 containing YAP1 targeting sequences (see Supplementary Table S1), pLX304-empty vector (RRID:Addgene_25890), pLX304-human YAP1-WT, and pLX304human YAP1–5SA were generously provided by David Barbie (DanaFarber Cancer Institute, Boston,MA). .. HEK293T cells were transfected with 1 mg lentivirus-based expression vectors and packaging plasmids using X-tremeGENE 9 transfection reagent (Roche) according to the manufacturer’s instructions.



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    Soft agar colony formation in cells overexpressing WT <t>Nudt2(PLX304</t> − V5 − Nudt2). Stable overexpression of WT Nudt2 was generated in CHL − 1 melanoma cell and the cells were seeded at 5000 cells/250 µL in 24-well plates for 21 days. ( A ). Expression of endogenous Nudt2, overexpressed Nudt2 (V5 − Nudt2) was analyzed via Western blot. ( B ). Representative photomicrographs: colonies were stained with p-iodonitrotetrazolium violet stain. ( C ). Colony numbers were counted using the Image J software(ij153-win-java8); n = 7. The data are represented as mean ± SEM. For statistical analysis, Wilcoxon signed-rank test was used. * p < 0.01.
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    Image Search Results


    Soft agar colony formation in cells overexpressing WT Nudt2(PLX304 − V5 − Nudt2). Stable overexpression of WT Nudt2 was generated in CHL − 1 melanoma cell and the cells were seeded at 5000 cells/250 µL in 24-well plates for 21 days. ( A ). Expression of endogenous Nudt2, overexpressed Nudt2 (V5 − Nudt2) was analyzed via Western blot. ( B ). Representative photomicrographs: colonies were stained with p-iodonitrotetrazolium violet stain. ( C ). Colony numbers were counted using the Image J software(ij153-win-java8); n = 7. The data are represented as mean ± SEM. For statistical analysis, Wilcoxon signed-rank test was used. * p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma

    doi: 10.3390/ijms241310513

    Figure Lengend Snippet: Soft agar colony formation in cells overexpressing WT Nudt2(PLX304 − V5 − Nudt2). Stable overexpression of WT Nudt2 was generated in CHL − 1 melanoma cell and the cells were seeded at 5000 cells/250 µL in 24-well plates for 21 days. ( A ). Expression of endogenous Nudt2, overexpressed Nudt2 (V5 − Nudt2) was analyzed via Western blot. ( B ). Representative photomicrographs: colonies were stained with p-iodonitrotetrazolium violet stain. ( C ). Colony numbers were counted using the Image J software(ij153-win-java8); n = 7. The data are represented as mean ± SEM. For statistical analysis, Wilcoxon signed-rank test was used. * p < 0.01.

    Article Snippet: 1 , Empty PLX304 vector (lentiviral-expressing vector) , # 25890, Addgene Plasmid , Used to prepare Control cells for overexpression system.

    Techniques: Over Expression, Generated, Expressing, Western Blot, Staining, Software

    The lists of constructs and vectors used in this study.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma

    doi: 10.3390/ijms241310513

    Figure Lengend Snippet: The lists of constructs and vectors used in this study.

    Article Snippet: 1 , Empty PLX304 vector (lentiviral-expressing vector) , # 25890, Addgene Plasmid , Used to prepare Control cells for overexpression system.

    Techniques: Construct, Plasmid Preparation, Over Expression, Expressing, Luciferase, In Vivo