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Soft agar colony formation in cells overexpressing WT <t>Nudt2(PLX304</t> − V5 − Nudt2). Stable overexpression of WT Nudt2 was generated in CHL − 1 melanoma cell and the cells were seeded at 5000 cells/250 µL in 24-well plates for 21 days. ( A ). Expression of endogenous Nudt2, overexpressed Nudt2 (V5 − Nudt2) was analyzed via Western blot. ( B ). Representative photomicrographs: colonies were stained with p-iodonitrotetrazolium violet stain. ( C ). Colony numbers were counted using the Image J software(ij153-win-java8); n = 7. The data are represented as mean ± SEM. For statistical analysis, Wilcoxon signed-rank test was used. * p < 0.01.
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Soft agar colony formation in cells overexpressing WT Nudt2(PLX304 − V5 − Nudt2). Stable overexpression of WT Nudt2 was generated in CHL − 1 melanoma cell and the cells were seeded at 5000 cells/250 µL in 24-well plates for 21 days. ( A ). Expression of endogenous Nudt2, overexpressed Nudt2 (V5 − Nudt2) was analyzed via Western blot. ( B ). Representative photomicrographs: colonies were stained with p-iodonitrotetrazolium violet stain. ( C ). Colony numbers were counted using the Image J software(ij153-win-java8); n = 7. The data are represented as mean ± SEM. For statistical analysis, Wilcoxon signed-rank test was used. * p < 0.01.

Journal: International Journal of Molecular Sciences

Article Title: Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma

doi: 10.3390/ijms241310513

Figure Lengend Snippet: Soft agar colony formation in cells overexpressing WT Nudt2(PLX304 − V5 − Nudt2). Stable overexpression of WT Nudt2 was generated in CHL − 1 melanoma cell and the cells were seeded at 5000 cells/250 µL in 24-well plates for 21 days. ( A ). Expression of endogenous Nudt2, overexpressed Nudt2 (V5 − Nudt2) was analyzed via Western blot. ( B ). Representative photomicrographs: colonies were stained with p-iodonitrotetrazolium violet stain. ( C ). Colony numbers were counted using the Image J software(ij153-win-java8); n = 7. The data are represented as mean ± SEM. For statistical analysis, Wilcoxon signed-rank test was used. * p < 0.01.

Article Snippet: 1 , Empty PLX304 vector (lentiviral-expressing vector) , # 25890, Addgene Plasmid , Used to prepare Control cells for overexpression system.

Techniques: Over Expression, Generated, Expressing, Western Blot, Staining, Software

The lists of constructs and vectors used in this study.

Journal: International Journal of Molecular Sciences

Article Title: Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma

doi: 10.3390/ijms241310513

Figure Lengend Snippet: The lists of constructs and vectors used in this study.

Article Snippet: 1 , Empty PLX304 vector (lentiviral-expressing vector) , # 25890, Addgene Plasmid , Used to prepare Control cells for overexpression system.

Techniques: Construct, Plasmid Preparation, Over Expression, Expressing, Luciferase, In Vivo