anti plin1  (Proteintech)

Journal: Advanced Science

Article Title: CGRP Enhances the Regeneration of Bone Defects by Regulating Bone Marrow Mesenchymal Stem Cells Through Promoting ANGPTL4 Secretion by Bone Blood Vessels

doi: 10.1002/advs.202522295

Figure Lengend Snippet: ANGPTL4 secreted by bone vessels restores bone formation by coupling with osteogenic differentiation of bone marrow mesenchymal stem cells. (A) RT‐qPCR analysis of Fabp4, Pparg, LPL, Id4, Adipoq, and perilipin expression levels in bone marrow mesenchymal stem cells (BMSCs) treated with different concentrations of recombinant Angptl4 protein. (B) Western blotting to determine Fabp4, Pparg, Lpl, Plin, Adipoq, Id4, and GAPDH expression levels in BMSCs treated with 50 ng/mL recombinant Angptl4 protein. n = 3 mice in each group. (C and D) Representative images of alizarin red S staining and quantification of calcification of BMSCs treated with recombinant Angptl4 protein. (E and F) Representative images of Alpl staining and quantification of Alpl activity in BMSCs treated with recombinant Angptl4 protein. (G and H) Representative images of oil red O staining and quantification of lipid formation by BMSCs treated with recombinant Angptl4 protein. (I–M) Representative micro‐CT images and quantitative micro‐CT analysis of the trabecular bone microarchitecture of WT mice injected with recombinant Angptl4 protein after bone defect introduction. (N) Representative images of hematoxylin–eosin staining in the bone regeneration area of the PBS and Angptl4 treatment groups. (O) Schematic diagram of the effects of CGRP. Scale bar, 1 mm. The data are shown as the mean ± standard deviation. * p < 0.05, ** p < 0.01; and *** p < 0.001 by Student's t ‐test. BV/TV, trabecular bone volume per tissue volume; Tb. N, trabecular number; Tb. Sp, trabecular separation; Tb. Th, trabecular thickness.

Article Snippet: The membranes were then blocked with 5% milk and incubated overnight with primary antibodies against p‐FAK (Tyr397) (Cell Signaling Technology, 3283), p‐FAK (Tyr576/577) (Cell Signaling Technology, 3281), p‐FAK (Tyr925) (Cell Signaling Technology, 3284), FAK (Cell Signaling Technology, 3285), p‐AKT (Ser473) (Cell Signaling Technology, 4060), AKT (Cell Signaling Technology, 9272), p‐ERK (Thr202/Tyr204) (Cell Signaling Technology, 9101), ERK (Cell Signaling Technology, 9102), VEGFA (Proteintech, 19003‐1‐AP), OPN (Proteintech, 22952‐1‐AP), BMP2 (Abcam, ab214821), Runx2 (Abcam, ab214821), β‐actin (Cell Signaling Technology, 4967), CGRP (Cell Signaling Technology, 14 959), Angptl4 (Proteintech, 18374‐1‐AP), Alpl (Proteintech, 11187‐1‐AP), Ocn (Proteintech, 16157‐1‐AP), Spp1 (Proteintech, 22952‐1‐AP), Sp7 (Proteintech, 28694‐1‐AP), Postn (Proteintech, 66491‐1‐Ig), Fabp4 (Proteintech, 12802‐1‐AP), Pparg (Proteintech, 16643‐1‐AP), Lpl (Proteintech, 28602‐1‐AP), Plin1 (Proteintech, 83905‐4‐RR), Adipoq (Proteintech, 83961‐4‐RR), Id4 (Proteintech, 21803‐1‐AP), GAPDH (Proteintech, 60004‐1‐Ig), and Tubulin (Cell Signaling Technology, 2146) at 4°C.

Techniques: Quantitative RT-PCR, Expressing, Recombinant, Western Blot, Staining, Activity Assay, Micro-CT, Injection, Standard Deviation

Journal: Molecular Metabolism

Article Title: The transcription factor CUX1 exerts opposing roles in human and mouse adipocyte differentiation

doi: 10.1016/j.molmet.2025.102290

Figure Lengend Snippet: Loss of Cux1 in mouse APCs enhances high fat diet-induced adipocyte differentiation. Control and KO mice harboring a tdTomato reporter gene were injected with tamoxifen (TMX) and fed a high fat diet (HFD) for 8 weeks. (A) Body weights. (B) Adipose tissue depot weights. (C) Immunofluorescence staining of PLIN1 (green) and tdTomato (new adipocytes, red) in iWAT (Top) and eWAT (Bottom). Scale 50 μm. (Right panels) Quantification of new adipocytes (tdTomato+; PLIN1+) as proportion of total adipocytes (PLIN1+). Values represent mean ± SEM, n > 5 sections from n = 5 animals per genotype. ( D , E ) H&E staining of iWAT (D) and eWAT (E). Scale 50 μm. (Right panels) Quantification of average adipocyte size in adipose tissue depots. Values represent mean ± SEM, n > 5 sections from n = 5 animals per genotype. Unpaired two-sided t-test. ∗P < 0.05.

Article Snippet: Sections were deparaffinized, rehydrated, and subjected to antigen retrieval before staining with anti-PLIN1 antibody (3470S, Cell Signaling Technology) and anti-tdTomato antibody (600-401-379, Rockland).

Techniques: Control, Injection, Immunofluorescence, Staining

Journal: Hereditas

Article Title: A combined transcriptomic, epigenetic, and functional analysis identifies novel biomarkers in breast cancer

doi: 10.1186/s41065-025-00630-1

Figure Lengend Snippet: Differential expression analysis and identification of hub genes in breast cancer. A Volcano plots showing significantly upregulated (red) and downregulated (blue) genes in three GEO datasets ( GSE42568 , GSE29431 , GSE21422 ). B Venn diagram showing 231 overlapping differentially expressed genes (DEGs) across the three datasets. C Protein–protein interaction (PPI) network of overlapping DEGs constructed using STRING and visualized in Cytoscape. D RT-qPCR analysis of PPARG, LEP, CD36, and PLIN1 expression in six breast cancer and five normal breast epithelial cell lines. E ROC curve analysis of hub genes showing their discriminatory power (AUC) between breast cancer and normal samples. F ELISA-based quantification of PPARG, LEP, CD36, and PLIN1 protein levels in the same cell lines, confirming reduced protein expression in cancer cells. P -value < 0.05

Article Snippet: The following TaqMan Assay IDs were used: PPARG (Hs01115513_m1), LEP (Hs00174877_m1), CD36 (Hs00354519_m1), PLIN1 (Hs00160173_m1), and GAPDH (Hs02758991_g1).

Techniques: Quantitative Proteomics, Construct, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Hereditas

Article Title: A combined transcriptomic, epigenetic, and functional analysis identifies novel biomarkers in breast cancer

doi: 10.1186/s41065-025-00630-1

Figure Lengend Snippet: Genomic alteration profiles of hub genes in breast cancer. A Oncoprint showing mutation frequency and types of hub genes in breast cancer samples (cBioPortal). B Heatmap of single nucleotide variant (SNV) percentages across hub genes. C Classification of mutation types, SNV classes, and mutation frequencies in hub genes. D Lollipop plots showing mutation positions across protein domains of PPARG, CD36, and PLIN1. E Pie charts illustrating copy number alterations (CNA) including amplifications and deletions for each hub gene. P -value < 0.05

Article Snippet: The following TaqMan Assay IDs were used: PPARG (Hs01115513_m1), LEP (Hs00174877_m1), CD36 (Hs00354519_m1), PLIN1 (Hs00160173_m1), and GAPDH (Hs02758991_g1).

Techniques: Mutagenesis, Variant Assay

Journal: Hereditas

Article Title: A combined transcriptomic, epigenetic, and functional analysis identifies novel biomarkers in breast cancer

doi: 10.1186/s41065-025-00630-1

Figure Lengend Snippet: Overexpression of CD36 and PLIN1 inhibits oncogenic behavior in MCF-7 cells. A , B mRNA and protein levels of CD36 and PLIN1 are significantly increased in MCF-7 cells transfected with expression vectors. C Overexpression significantly reduces proliferation ( p < 0.001). D , E Colony formation is markedly suppressed. F , G Wound closure is reduced, indicating decreased cell motility. P ***-value < 0.001

Article Snippet: The following TaqMan Assay IDs were used: PPARG (Hs01115513_m1), LEP (Hs00174877_m1), CD36 (Hs00354519_m1), PLIN1 (Hs00160173_m1), and GAPDH (Hs02758991_g1).

Techniques: Over Expression, Transfection, Expressing

Journal: Hereditas

Article Title: A combined transcriptomic, epigenetic, and functional analysis identifies novel biomarkers in breast cancer

doi: 10.1186/s41065-025-00630-1

Figure Lengend Snippet: CD36 and PLIN1 overexpression reduces proliferation and migration in T47D cells. A , B Confirmation of CD36 and PLIN1 overexpression at transcript and protein levels. C Cell proliferation is significantly inhibited in both overexpression groups. D , E Fewer colonies are formed, indicating reduced clonogenic capacity. F , G Migration is impaired in wound healing assays at 24 h. P ***-value < 0.001

Article Snippet: The following TaqMan Assay IDs were used: PPARG (Hs01115513_m1), LEP (Hs00174877_m1), CD36 (Hs00354519_m1), PLIN1 (Hs00160173_m1), and GAPDH (Hs02758991_g1).

Techniques: Over Expression, Migration