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Structured Review

Millipore pki5–24
Pki5–24, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pki5%E2%80%9324/pki5+24/10__1096_slash_fj__201802354r-100-44-48
Average 90 stars, based on 1 article reviews
pki5–24 - by Bioz Stars, 2026-10
90/100 stars

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Incubation:

Article Title: Protein Kinase A Phosphorylation of the Cardiac Calcium Release Channel (Ryanodine Receptor) in Normal and Failing Hearts
Article Snippet: Protein A-Sepharose beads were added, incubated at 4 °C for 1 h, washed with 1 phosphorylation buffer (8 mM MgCl2, 10 mM EGTA, and 50 mM Tris/PIPES, pH 6.8), and resuspended in 10 l of a 1.5 phosphorylation buffer containing either PKA catalytic subunit (Sigma Chemical Co., St. Louis, MO) or PKA plus a PKA inhibitor (PKI5–24, 500 nM, Calbiochem, San Diego, CA).

Article Title: Targeting RyR2 with a phosphorylation site–specific nanobody reverses dysfunction of failing cardiomyocytes in rats
Article Snippet: The samples were incubatedwith proteinA–sepharose beads for 1 h and then transferred into 10 ml of 1.53 phosphorylation buffer [8 mM MgCl2, 10 mM EGTA, 50 mM Tris, and PIPES (pH 6.8)] with either the PKA catalytic subunit (MilliporeSigma, Burlington, MA, USA) or the inhibitor PKI5–24 (500 nM; Calbiochem, San Diego, CA, USA)with or without different nanobody fragments (10 mg/ml).

Article Title: Kinase-dependent regulation of the intermediate conductance, calcium-dependent potassium channel, hIK1.
Article Snippet: Chemicals—Ionomycin, forskolin, AMP-PNP, AMP-PCP, GTP, PKI5– 24, CaMKII281–309, PKC19–31, and H-89 were purchased from Calbiochem.

Article Title: Targeting RyR2 with a phosphorylation site-specific nanobody Reverses Dysfunction of Failing Cardiomyocytes in Rat
Article Snippet: Samples were incubation with protein A-Sepharose beads for 1h and then transferred into 10 μl of 1.5× phosphorylation buffer (8 mM MgCl 2 /10 mM EGTA/50 mM Tris/Pipes, pH 6.8) added either PKA catalytic subunit (Sigma, St.Louis, MO) or inhibitor PKI5–24 (500 nM, Calbiochem, San Diego) with or without different nanobody fragments (10 ug/ml).

Phospho-proteomics:

Article Title: Protein Kinase A Phosphorylation of the Cardiac Calcium Release Channel (Ryanodine Receptor) in Normal and Failing Hearts
Article Snippet: Protein A-Sepharose beads were added, incubated at 4 °C for 1 h, washed with 1 phosphorylation buffer (8 mM MgCl2, 10 mM EGTA, and 50 mM Tris/PIPES, pH 6.8), and resuspended in 10 l of a 1.5 phosphorylation buffer containing either PKA catalytic subunit (Sigma Chemical Co., St. Louis, MO) or PKA plus a PKA inhibitor (PKI5–24, 500 nM, Calbiochem, San Diego, CA).

Article Title: Targeting RyR2 with a phosphorylation site–specific nanobody reverses dysfunction of failing cardiomyocytes in rats
Article Snippet: The samples were incubatedwith proteinA–sepharose beads for 1 h and then transferred into 10 ml of 1.53 phosphorylation buffer [8 mM MgCl2, 10 mM EGTA, 50 mM Tris, and PIPES (pH 6.8)] with either the PKA catalytic subunit (MilliporeSigma, Burlington, MA, USA) or the inhibitor PKI5–24 (500 nM; Calbiochem, San Diego, CA, USA)with or without different nanobody fragments (10 mg/ml).

Article Title: Kinase-dependent regulation of the intermediate conductance, calcium-dependent potassium channel, hIK1.
Article Snippet: Chemicals—Ionomycin, forskolin, AMP-PNP, AMP-PCP, GTP, PKI5– 24, CaMKII281–309, PKC19–31, and H-89 were purchased from Calbiochem.

Article Title: Targeting RyR2 with a phosphorylation site-specific nanobody Reverses Dysfunction of Failing Cardiomyocytes in Rat
Article Snippet: Samples were incubation with protein A-Sepharose beads for 1h and then transferred into 10 μl of 1.5× phosphorylation buffer (8 mM MgCl 2 /10 mM EGTA/50 mM Tris/Pipes, pH 6.8) added either PKA catalytic subunit (Sigma, St.Louis, MO) or inhibitor PKI5–24 (500 nM, Calbiochem, San Diego) with or without different nanobody fragments (10 ug/ml).



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