pinp elisa kit  (Cusabio)

Journal: Frontiers in Endocrinology

Article Title: Anoctamin 5 mutation leads to abnormal bone homeostasis of GDD by regulating AMPK-dependent glucose metabolism

doi: 10.3389/fendo.2026.1703491

Figure Lengend Snippet: AMPK inhibitor regulates osteogenesis and osteoclastogenesis of GDD in vivo . 12-week-old male mice were used to observe bone phenotype. (A) Representative photomicrographs and quantitative immuno-positive area analysis of OCN in tibia (bar=100 μm); (B–D) ELISA analysis of serum level of ALP, PINP (B) , OPG, OPG/RANKL (C) , and CTX (D, E) Representative images of TRAP staining and quantitative analysis of the osteoclast number (Oc.N)/perimeter of bone (B.Pm) in the germinal center of the tibia (bar=100 μm). Data are presented as mean ± SEM. Statistic significances are determined by one-way ANOVAs with Dunnett’s multiple comparison tests, with ns: no significance, *P < 0.05, **P < 0.01.

Article Snippet: Procollagen I N-terminal propeptide (PINP) and type I collagen cross-linked C-telopeptide (CTX) in the serum were measured by using mouse PINP ELISA Kit (Elabscience, E-EL-M0233) and mouse β-CTx ELISA Kit (Elabscience, E-EL-M0372) following the manufacturer’s protocol.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Staining, Comparison

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: The differences of optimal vibration frequency for accelerating OTM between the younger group and the older group (A and B) Micro-CT images and statistical analysis showed the orthodontic teeth of the younger rats in L-Vib group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (C) The content of CTX-1 in serum of the younger rats in control group, L-Vib group and H-Vib group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. (D and E) Representative images of TRAP staining of alveolar bone of the younger rats in control group, L-Vib group and H-Vib group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (F) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the younger rats in control group, L-Vib group and H-Vib group. Scale bar: 100 μm. (G) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in L-Vib group were higher than those in control group and H-Vib group. Mean ± SD. n = 3. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (H and I) Micro-CT images and statistical analysis showed the orthodontic teeth of the older rats in H-Vib group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5. ∗ p < 0.05. ∗∗∗ p < 0.001. (J) The content of CTX-1 in serum of the older rats in control group, L-Vib group and H-Vib group was detected by ELISA. Mean ± SD. n = 3–4. ∗ p < 0.05. ∗∗ p < 0.01. (K and L) Representative images of TRAP staining of alveolar bone of the older rats in control group, L-Vib group and H-Vib group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗∗ p < 0.001. (M) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the older rats in control group, L-Vib group and H-Vib group. Scale bar: 100 μm. (N) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in H-Vib group were higher than those in control group and L-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. Statistical significance was determined using the one-way ANOVA.

Article Snippet: PINP ELISA kit , CUSABIO , CSB-E12774r.

Techniques: Micro-CT, Control, Enzyme-linked Immunosorbent Assay, Staining, Expressing

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: The synergistic effect of vibration and NIR light on OTM (A) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the younger rats in control group, L-Vib group and synergy group. Scale bar: 100 μm. (B) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in synergy group were higher than those in control group and L-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (C and D) Representative images of TRAP staining of alveolar bone of the younger rats in control group, L-Vib group and synergy group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4. ∗ p < 0.05. ∗∗∗ p < 0.001. (E) The content of CTX-1 in serum of the younger rats in control group, L-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. (F and G) Micro-CT images and statistical analysis showed the orthodontic teeth of the younger rats in synergy group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5–6. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (H) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the older rats in control group, H-Vib group and synergy group. Scale bar: 100 μm. (I) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in synergy group were higher than those in control group and H-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (J and K) Representative images of TRAP staining of alveolar bone of the older rats in control group, H-Vib group and synergy group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (L) The content of CTX-1 in serum of the older rats in control group, H-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗∗ p < 0.001. (M and N) Micro-CT images and statistical analysis showed the orthodontic teeth of the older rats in synergy group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5–6. ∗∗∗ p < 0.001. Statistical significance was determined using the one-way ANOVA.

Article Snippet: PINP ELISA kit , CUSABIO , CSB-E12774r.

Techniques: Control, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Micro-CT

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: Light-vibration coordination accelerates OTM without root resorption and alveolar bone resorption (A and B) Micro-CT images and statistical analysis showed that the moving distance of orthodontic tooth was increased in synergy group of the younger rats after orthodontic force application for 4 weeks. Scale bar: 2 mm. Mean ± SD. n = 5. ∗∗ p < 0.01. (C) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in synergy group were higher than those in control group after orthodontic force application for 4 weeks. Mean ± SD. n = 3. ∗∗∗ p < 0.001. (D and E) Micro-CT images and statistical analysis showed that the moving distance of orthodontic tooth was increased in synergy group of the older rats after orthodontic force application for 4 weeks. Scale bar: 2 mm. Mean ± SD. n = 6. ∗∗ p < 0.01. (F) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in synergy group were higher than those in control group after orthodontic force application for 4 weeks. Mean ± SD. n = 3. ∗∗ p < 0.01. (G) Representative images of HE staining of the longitudinal sections of the maxillary first molar of the younger rats. Scale bar: 500 μm. (H and I) There was no significant difference in the level of root resorption and alveolar bone resorption between the control group and the synergy group of the younger rats. Mean ± SD. n = 4. ns, not significant. (J) The content of PINP in serum of the younger rats in control group, L-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3–4. ∗∗∗ p < 0.001. (K) WB results showed that the protein expression levels of RUNX2 and OSX of the younger rats in synergy group were higher than those in control group. Mean ± SD. n = 3. ∗∗ p < 0.01. (L) Representative images of HE staining of the longitudinal sections of the maxillary first molar of the older rats. Scale bar: 500 μm. (M and N) There was no significant difference in the level of root resorption and alveolar bone resorption between the control group and the synergy group of the older rats. Mean ± SD. n = 3–4. ns, not significant. (O) The content of PINP in serum of the older rats in control group, H-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3–4. ∗∗∗ p < 0.001. (P) WB results showed that the protein expression levels of RUNX2 and OSX of the older rats in synergy group were higher than those in control group. Mean ± SD. n = 3. ∗∗ p < 0.01. Statistical significance was determined using the Student’s t tests one-way ANOVA.

Article Snippet: PINP ELISA kit , CUSABIO , CSB-E12774r.

Techniques: Micro-CT, Expressing, Control, Staining, Enzyme-linked Immunosorbent Assay

Journal: Cell Death & Disease

Article Title: Disrupted bone microenvironment and immune recovery following total body irradiation in a murine model

doi: 10.1038/s41419-025-08303-7

Figure Lengend Snippet: A Schematic diagram showing experiment design of the long-term experiment. Irradiation was performed in 9-week-old mice that were euthanized after an additional 1 day (1d), 2-, 6-, or 12 weeks [12w]. B Changes in body weight over the 12-week experiment post-irradiation. A significant difference was observed using the Student’s t test 1 week after irradiation and HSCT, at 10 weeks of age. The dotted line indicates the time of irradiation and HSCT. C Total body areal bone mineral density (aBMD), and lumbar spine vertebrae (LS) aBMD in irradiated and naive mice. A significant difference was observed with the Student’s t test 4 weeks after irradiation and HSCT, and this difference persisted throughout the entire 12-week period in mice aged 13–21 weeks. D Micro-computed tomography (μCT) analysis of trabecular bone volume per total volume (Tb. BV/TV) in the femur, shown as relative change versus naive mice, which is set to 100%. E Relative changes compared to naive mice (set to 100%) of trabecular number (Tb.N), trabecular separation (Tb.Sp), and trabecular thickness (Tb.Th) in the femur. F Representative images of trabecular bone in the femur. The scale bar represents 200 μm. G μCT analyses of cortical thickness (Ct.Th) in femur, shown as relative change versus naive mice, which is set to 100%. H Serum levels of C-terminal telopeptide (CTX, a bone resorption marker) and procollagen type I N-terminal propeptide (PINP, a bone formation marker). Statistical differences between irradiated and HSCT compared to naive mice at each time point were evaluated using Student’s t test. Sample sizes ranged from n = 6–12. Data are presented as mean ± SD. Significance levels are indicated as * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Enzyme-linked immunosorbent assays (ELISAs) were conducted to quantify C-terminal type I collagen fragments (CTX-I), indicative of bone resorption, (Immunodiagnostic Systems, East Boldon, UK), procollagen type I N pro-peptide (PINP), a serum marker for bone formation (Immunodiagnostic Systems), and immune cytokines IL-6 (Invitrogen) and TGF-β1 (Invitrogen).

Techniques: Irradiation, Micro-CT, Marker