Review



p pink1 s228  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Proteintech p pink1 s228
    High uric acid (HUA) induces mitophagy in hepatocytes . A , representative Western blot (WB) images of <t>PINK1,</t> LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 6, 12, 24, and 48 h). B – D , quantification of PINK1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). E , representative WB images of P62, P-PINK1 <t>S228</t> , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 3, 6, 9, and 12 h). F – M , quantification of P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). N , representative WB images of P62, P-PINK1 S228 , and PINK1 protein levels in the mitochondrial fraction of HepG2 cells following HUA treatment at various time points (0, 3, 6, and 12 h). O – Q , quantification of mitochondrial P62, P-PINK1, and PINK1 protein levels in HepG2 cells (n = 3). R , representative WB images of UB protein expression in primary mouse hepatocytes treated with or without HUA and MG132 (10 μM) for 9 h. S , RT–PCR analysis of P62, PINK1, Parkin, MFN1, MFN2, OPA1, DRP1, and VDAC1 mRNA expression in primary hepatocytes treated with HUA for different durations (0, 3, 6, and 9 h). T , RT–PCR analysis of PGC1A, NRF1, and TFAM mRNA expression in primary hepatocytes treated with HUA for different durations (0, 6, 12, and 24 h). U , representative confocal microscopy images showing LC3B ( red ) and TOM20 ( green ) colocalization in primary hepatocytes treated with HUA for 9 h. Nuclei were counterstained with DAPI ( blue ). The scale bar represents 20 μm. V , quantification of LC3B mean fluorescence intensity (n = 3). W , Pearson’s correlation coefficient between LC3B and TOM20 from experiments shown in U (n = 3). Individual data points represent each independent experiment. Data are expressed as mean ± SD. Statistical analysis was done using unpaired t test and one-way ANOVA, Tukey’s post hoc analysis. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 compared with the indicated groups. DAPI, 4',6-diamidino-2-phenylindole.
    P Pink1 S228, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 622 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p pink1 s228/product/Proteintech
    Average 96 stars, based on 622 article reviews
    p pink1 s228 - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Activation of mitophagy antagonizes high uric acid–induced hepatic lipid accumulation"

    Article Title: Activation of mitophagy antagonizes high uric acid–induced hepatic lipid accumulation

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2025.111054

    High uric acid (HUA) induces mitophagy in hepatocytes . A , representative Western blot (WB) images of PINK1, LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 6, 12, 24, and 48 h). B – D , quantification of PINK1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). E , representative WB images of P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 3, 6, 9, and 12 h). F – M , quantification of P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). N , representative WB images of P62, P-PINK1 S228 , and PINK1 protein levels in the mitochondrial fraction of HepG2 cells following HUA treatment at various time points (0, 3, 6, and 12 h). O – Q , quantification of mitochondrial P62, P-PINK1, and PINK1 protein levels in HepG2 cells (n = 3). R , representative WB images of UB protein expression in primary mouse hepatocytes treated with or without HUA and MG132 (10 μM) for 9 h. S , RT–PCR analysis of P62, PINK1, Parkin, MFN1, MFN2, OPA1, DRP1, and VDAC1 mRNA expression in primary hepatocytes treated with HUA for different durations (0, 3, 6, and 9 h). T , RT–PCR analysis of PGC1A, NRF1, and TFAM mRNA expression in primary hepatocytes treated with HUA for different durations (0, 6, 12, and 24 h). U , representative confocal microscopy images showing LC3B ( red ) and TOM20 ( green ) colocalization in primary hepatocytes treated with HUA for 9 h. Nuclei were counterstained with DAPI ( blue ). The scale bar represents 20 μm. V , quantification of LC3B mean fluorescence intensity (n = 3). W , Pearson’s correlation coefficient between LC3B and TOM20 from experiments shown in U (n = 3). Individual data points represent each independent experiment. Data are expressed as mean ± SD. Statistical analysis was done using unpaired t test and one-way ANOVA, Tukey’s post hoc analysis. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 compared with the indicated groups. DAPI, 4',6-diamidino-2-phenylindole.
    Figure Legend Snippet: High uric acid (HUA) induces mitophagy in hepatocytes . A , representative Western blot (WB) images of PINK1, LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 6, 12, 24, and 48 h). B – D , quantification of PINK1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). E , representative WB images of P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 3, 6, 9, and 12 h). F – M , quantification of P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). N , representative WB images of P62, P-PINK1 S228 , and PINK1 protein levels in the mitochondrial fraction of HepG2 cells following HUA treatment at various time points (0, 3, 6, and 12 h). O – Q , quantification of mitochondrial P62, P-PINK1, and PINK1 protein levels in HepG2 cells (n = 3). R , representative WB images of UB protein expression in primary mouse hepatocytes treated with or without HUA and MG132 (10 μM) for 9 h. S , RT–PCR analysis of P62, PINK1, Parkin, MFN1, MFN2, OPA1, DRP1, and VDAC1 mRNA expression in primary hepatocytes treated with HUA for different durations (0, 3, 6, and 9 h). T , RT–PCR analysis of PGC1A, NRF1, and TFAM mRNA expression in primary hepatocytes treated with HUA for different durations (0, 6, 12, and 24 h). U , representative confocal microscopy images showing LC3B ( red ) and TOM20 ( green ) colocalization in primary hepatocytes treated with HUA for 9 h. Nuclei were counterstained with DAPI ( blue ). The scale bar represents 20 μm. V , quantification of LC3B mean fluorescence intensity (n = 3). W , Pearson’s correlation coefficient between LC3B and TOM20 from experiments shown in U (n = 3). Individual data points represent each independent experiment. Data are expressed as mean ± SD. Statistical analysis was done using unpaired t test and one-way ANOVA, Tukey’s post hoc analysis. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 compared with the indicated groups. DAPI, 4',6-diamidino-2-phenylindole.

    Techniques Used: Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Confocal Microscopy, Fluorescence

    High uric acid induces mitophagy in mouse liver . A , representative Wesetrn blot (WB) images showing the expression of mitophagy-related proteins (P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, and LC3B-II/I), mitochondrial fusion proteins MFN2 (mitofusin 2), and the mitochondrial marker COXIV in liver tissues from WT and Uox -KO mice. B – J , quantification of MFN2, P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B-II/I, and COXIV protein levels (n = 3–5). K , RT–PCR analysis of mitochondrial dynamics–related gene expression in liver tissues from WT and Uox -KO mice (n = 4–8). L , gene set enrichment analysis (GSEA) plot for the “mitochondrial outer membrane” Gene Ontology term. Individual data points on the graph represent data from each mouse. Data are presented as mean ± SD. Statistical analysis was done using unpaired t test and one-way ANOVA, Tukey’s post hoc analysis. ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 compared with the indicated groups.
    Figure Legend Snippet: High uric acid induces mitophagy in mouse liver . A , representative Wesetrn blot (WB) images showing the expression of mitophagy-related proteins (P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, and LC3B-II/I), mitochondrial fusion proteins MFN2 (mitofusin 2), and the mitochondrial marker COXIV in liver tissues from WT and Uox -KO mice. B – J , quantification of MFN2, P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B-II/I, and COXIV protein levels (n = 3–5). K , RT–PCR analysis of mitochondrial dynamics–related gene expression in liver tissues from WT and Uox -KO mice (n = 4–8). L , gene set enrichment analysis (GSEA) plot for the “mitochondrial outer membrane” Gene Ontology term. Individual data points on the graph represent data from each mouse. Data are presented as mean ± SD. Statistical analysis was done using unpaired t test and one-way ANOVA, Tukey’s post hoc analysis. ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 compared with the indicated groups.

    Techniques Used: Expressing, Marker, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Membrane

    Schematic diagram of activation of mitophagy antagonizes high uric acid–induced hepatic lipid accumulation (created with Figdraw.com ) . High uric acid upregulates CD36, promoting hepatic fat accumulation. Meanwhile, it activates mitophagy via the PINK1–Parkin pathway, which antagonizes this lipid accumulation.
    Figure Legend Snippet: Schematic diagram of activation of mitophagy antagonizes high uric acid–induced hepatic lipid accumulation (created with Figdraw.com ) . High uric acid upregulates CD36, promoting hepatic fat accumulation. Meanwhile, it activates mitophagy via the PINK1–Parkin pathway, which antagonizes this lipid accumulation.

    Techniques Used: Activation Assay



    Similar Products

    gene exp pink1 mm00550827 m1  (Thermo Fisher)
    91
    Thermo Fisher gene exp pink1 mm00550827 m1
    Gene Exp Pink1 Mm00550827 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp pink1 mm00550827 m1/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    gene exp pink1 mm00550827 m1 - by Bioz Stars, 2026-03
    91/100 stars
    		  Buy from Supplier
    pink1 agonist  (MedChemExpress)
    94
    MedChemExpress pink1 agonist
    Pink1 Agonist, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pink1 agonist/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    pink1 agonist - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    p pink1 s228  (Proteintech)
    96
    Proteintech p pink1 s228
    High uric acid (HUA) induces mitophagy in hepatocytes . A , representative Western blot (WB) images of <t>PINK1,</t> LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 6, 12, 24, and 48 h). B – D , quantification of PINK1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). E , representative WB images of P62, P-PINK1 <t>S228</t> , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 3, 6, 9, and 12 h). F – M , quantification of P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). N , representative WB images of P62, P-PINK1 S228 , and PINK1 protein levels in the mitochondrial fraction of HepG2 cells following HUA treatment at various time points (0, 3, 6, and 12 h). O – Q , quantification of mitochondrial P62, P-PINK1, and PINK1 protein levels in HepG2 cells (n = 3). R , representative WB images of UB protein expression in primary mouse hepatocytes treated with or without HUA and MG132 (10 μM) for 9 h. S , RT–PCR analysis of P62, PINK1, Parkin, MFN1, MFN2, OPA1, DRP1, and VDAC1 mRNA expression in primary hepatocytes treated with HUA for different durations (0, 3, 6, and 9 h). T , RT–PCR analysis of PGC1A, NRF1, and TFAM mRNA expression in primary hepatocytes treated with HUA for different durations (0, 6, 12, and 24 h). U , representative confocal microscopy images showing LC3B ( red ) and TOM20 ( green ) colocalization in primary hepatocytes treated with HUA for 9 h. Nuclei were counterstained with DAPI ( blue ). The scale bar represents 20 μm. V , quantification of LC3B mean fluorescence intensity (n = 3). W , Pearson’s correlation coefficient between LC3B and TOM20 from experiments shown in U (n = 3). Individual data points represent each independent experiment. Data are expressed as mean ± SD. Statistical analysis was done using unpaired t test and one-way ANOVA, Tukey’s post hoc analysis. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 compared with the indicated groups. DAPI, 4',6-diamidino-2-phenylindole.
    P Pink1 S228, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p pink1 s228/product/Proteintech
    Average 96 stars, based on 1 article reviews
    p pink1 s228 - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    pink1  (Proteintech)
    96
    Proteintech pink1
    TA activated mitophagy by <t>PINK1/Parkin</t> signaling pathway. A The Western blotting detection of the protein expressions of LC3I and LC3II in HT22 cells treated with TA and Rap (20 μM). B The bar chart presents the rate of LC3II/LC3I, * p < 0.05, * p < 0.01 and *** p < 0.01 versus the control group, n = 3. C Representative images of stable GFP-LC3 U87 cells treated with or without TA, Magnification: 20 × , scale bar: 100 μm. D The bar chart presents the rate of cells with GFP-LC3 puncta formation in stable GFP-LC3 U87 cells, * p < 0.05 and *** p < 0.001 versus control group. E Representative images of stable stable RFP-GFP-LC3 U87 cells treated with or without TA, Baf (100 nM) or Rap (10 μM), Magnification: × 64, scale bar: 5 μm. F Representative images of the co-localization of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells with or without TA, CCCP or TA+AC220. G The bar chart indicates the rate of GFP/RFP, ** p < 0.05 and *** p < 0.001. H – K Line scan analysis showing the co-localization intensity (a.u.) of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells under different treatments. L Representative images of HT22 cells transiently transfecting Mito-QC plasmid and treated with or without TA, CCCP or TA+AC220. M Representative images of BV2 cells transiently transfecting Mito-QC and treated with or without TA, CCCP or TA+AC220. N The bar chart indicates the rate of RFP/GFP in HT22 ransiently transfecting Mito-QC cells. ** p < 0.01 and *** p < 0.001 versus the control group, n = 3. O The bar chart indicates the rate of RFP/GFP in BV2 ransiently transfecting Mito-QC cells. ** p <0.01 and *** p < 0.001 versus the control group, n = 3. P Western blot detection of Parkin, p-Parkin, and PINK1 protein expression in HT22 cells treated with or without TA. Q , R The bar chart presents the rate of p-Parkin/Parkin (n = 3), PINK1/GAPDH (n = 5). ** p < 0.01 and *** p < 0.001 versus the control group
    Pink1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pink1/product/Proteintech
    Average 96 stars, based on 1 article reviews
    pink1 - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    resource source identifier anti-lc3b abclonol a19665  (Proteintech)
    96
    Proteintech resource source identifier anti-lc3b abclonol a19665
    TA activated mitophagy by <t>PINK1/Parkin</t> signaling pathway. A The Western blotting detection of the protein expressions of LC3I and LC3II in HT22 cells treated with TA and Rap (20 μM). B The bar chart presents the rate of LC3II/LC3I, * p < 0.05, * p < 0.01 and *** p < 0.01 versus the control group, n = 3. C Representative images of stable GFP-LC3 U87 cells treated with or without TA, Magnification: 20 × , scale bar: 100 μm. D The bar chart presents the rate of cells with GFP-LC3 puncta formation in stable GFP-LC3 U87 cells, * p < 0.05 and *** p < 0.001 versus control group. E Representative images of stable stable RFP-GFP-LC3 U87 cells treated with or without TA, Baf (100 nM) or Rap (10 μM), Magnification: × 64, scale bar: 5 μm. F Representative images of the co-localization of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells with or without TA, CCCP or TA+AC220. G The bar chart indicates the rate of GFP/RFP, ** p < 0.05 and *** p < 0.001. H – K Line scan analysis showing the co-localization intensity (a.u.) of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells under different treatments. L Representative images of HT22 cells transiently transfecting Mito-QC plasmid and treated with or without TA, CCCP or TA+AC220. M Representative images of BV2 cells transiently transfecting Mito-QC and treated with or without TA, CCCP or TA+AC220. N The bar chart indicates the rate of RFP/GFP in HT22 ransiently transfecting Mito-QC cells. ** p < 0.01 and *** p < 0.001 versus the control group, n = 3. O The bar chart indicates the rate of RFP/GFP in BV2 ransiently transfecting Mito-QC cells. ** p <0.01 and *** p < 0.001 versus the control group, n = 3. P Western blot detection of Parkin, p-Parkin, and PINK1 protein expression in HT22 cells treated with or without TA. Q , R The bar chart presents the rate of p-Parkin/Parkin (n = 3), PINK1/GAPDH (n = 5). ** p < 0.01 and *** p < 0.001 versus the control group
    Resource Source Identifier Anti Lc3b Abclonol A19665, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resource source identifier anti-lc3b abclonol a19665/product/Proteintech
    Average 96 stars, based on 1 article reviews
    resource source identifier anti-lc3b abclonol a19665 - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    rabbit 25 pink1 23274 1 ap proteintech  (Proteintech)
    96
    Proteintech rabbit 25 pink1 23274 1 ap proteintech
    TA activated mitophagy by <t>PINK1/Parkin</t> signaling pathway. A The Western blotting detection of the protein expressions of LC3I and LC3II in HT22 cells treated with TA and Rap (20 μM). B The bar chart presents the rate of LC3II/LC3I, * p < 0.05, * p < 0.01 and *** p < 0.01 versus the control group, n = 3. C Representative images of stable GFP-LC3 U87 cells treated with or without TA, Magnification: 20 × , scale bar: 100 μm. D The bar chart presents the rate of cells with GFP-LC3 puncta formation in stable GFP-LC3 U87 cells, * p < 0.05 and *** p < 0.001 versus control group. E Representative images of stable stable RFP-GFP-LC3 U87 cells treated with or without TA, Baf (100 nM) or Rap (10 μM), Magnification: × 64, scale bar: 5 μm. F Representative images of the co-localization of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells with or without TA, CCCP or TA+AC220. G The bar chart indicates the rate of GFP/RFP, ** p < 0.05 and *** p < 0.001. H – K Line scan analysis showing the co-localization intensity (a.u.) of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells under different treatments. L Representative images of HT22 cells transiently transfecting Mito-QC plasmid and treated with or without TA, CCCP or TA+AC220. M Representative images of BV2 cells transiently transfecting Mito-QC and treated with or without TA, CCCP or TA+AC220. N The bar chart indicates the rate of RFP/GFP in HT22 ransiently transfecting Mito-QC cells. ** p < 0.01 and *** p < 0.001 versus the control group, n = 3. O The bar chart indicates the rate of RFP/GFP in BV2 ransiently transfecting Mito-QC cells. ** p <0.01 and *** p < 0.001 versus the control group, n = 3. P Western blot detection of Parkin, p-Parkin, and PINK1 protein expression in HT22 cells treated with or without TA. Q , R The bar chart presents the rate of p-Parkin/Parkin (n = 3), PINK1/GAPDH (n = 5). ** p < 0.01 and *** p < 0.001 versus the control group
    Rabbit 25 Pink1 23274 1 Ap Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit 25 pink1 23274 1 ap proteintech/product/Proteintech
    Average 96 stars, based on 1 article reviews
    rabbit 25 pink1 23274 1 ap proteintech - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    anti pink1 antibody  (Proteintech)
    96
    Proteintech anti pink1 antibody
    TA activated mitophagy by <t>PINK1/Parkin</t> signaling pathway. A The Western blotting detection of the protein expressions of LC3I and LC3II in HT22 cells treated with TA and Rap (20 μM). B The bar chart presents the rate of LC3II/LC3I, * p < 0.05, * p < 0.01 and *** p < 0.01 versus the control group, n = 3. C Representative images of stable GFP-LC3 U87 cells treated with or without TA, Magnification: 20 × , scale bar: 100 μm. D The bar chart presents the rate of cells with GFP-LC3 puncta formation in stable GFP-LC3 U87 cells, * p < 0.05 and *** p < 0.001 versus control group. E Representative images of stable stable RFP-GFP-LC3 U87 cells treated with or without TA, Baf (100 nM) or Rap (10 μM), Magnification: × 64, scale bar: 5 μm. F Representative images of the co-localization of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells with or without TA, CCCP or TA+AC220. G The bar chart indicates the rate of GFP/RFP, ** p < 0.05 and *** p < 0.001. H – K Line scan analysis showing the co-localization intensity (a.u.) of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells under different treatments. L Representative images of HT22 cells transiently transfecting Mito-QC plasmid and treated with or without TA, CCCP or TA+AC220. M Representative images of BV2 cells transiently transfecting Mito-QC and treated with or without TA, CCCP or TA+AC220. N The bar chart indicates the rate of RFP/GFP in HT22 ransiently transfecting Mito-QC cells. ** p < 0.01 and *** p < 0.001 versus the control group, n = 3. O The bar chart indicates the rate of RFP/GFP in BV2 ransiently transfecting Mito-QC cells. ** p <0.01 and *** p < 0.001 versus the control group, n = 3. P Western blot detection of Parkin, p-Parkin, and PINK1 protein expression in HT22 cells treated with or without TA. Q , R The bar chart presents the rate of p-Parkin/Parkin (n = 3), PINK1/GAPDH (n = 5). ** p < 0.01 and *** p < 0.001 versus the control group
    Anti Pink1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pink1 antibody/product/Proteintech
    Average 96 stars, based on 1 article reviews
    anti pink1 antibody - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    anti pink1  (Proteintech)
    96
    Proteintech anti pink1
    TA activated mitophagy by <t>PINK1/Parkin</t> signaling pathway. A The Western blotting detection of the protein expressions of LC3I and LC3II in HT22 cells treated with TA and Rap (20 μM). B The bar chart presents the rate of LC3II/LC3I, * p < 0.05, * p < 0.01 and *** p < 0.01 versus the control group, n = 3. C Representative images of stable GFP-LC3 U87 cells treated with or without TA, Magnification: 20 × , scale bar: 100 μm. D The bar chart presents the rate of cells with GFP-LC3 puncta formation in stable GFP-LC3 U87 cells, * p < 0.05 and *** p < 0.001 versus control group. E Representative images of stable stable RFP-GFP-LC3 U87 cells treated with or without TA, Baf (100 nM) or Rap (10 μM), Magnification: × 64, scale bar: 5 μm. F Representative images of the co-localization of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells with or without TA, CCCP or TA+AC220. G The bar chart indicates the rate of GFP/RFP, ** p < 0.05 and *** p < 0.001. H – K Line scan analysis showing the co-localization intensity (a.u.) of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells under different treatments. L Representative images of HT22 cells transiently transfecting Mito-QC plasmid and treated with or without TA, CCCP or TA+AC220. M Representative images of BV2 cells transiently transfecting Mito-QC and treated with or without TA, CCCP or TA+AC220. N The bar chart indicates the rate of RFP/GFP in HT22 ransiently transfecting Mito-QC cells. ** p < 0.01 and *** p < 0.001 versus the control group, n = 3. O The bar chart indicates the rate of RFP/GFP in BV2 ransiently transfecting Mito-QC cells. ** p <0.01 and *** p < 0.001 versus the control group, n = 3. P Western blot detection of Parkin, p-Parkin, and PINK1 protein expression in HT22 cells treated with or without TA. Q , R The bar chart presents the rate of p-Parkin/Parkin (n = 3), PINK1/GAPDH (n = 5). ** p < 0.01 and *** p < 0.001 versus the control group
    Anti Pink1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pink1/product/Proteintech
    Average 96 stars, based on 1 article reviews
    anti pink1 - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    pink1  (Bioss)
    94
    Bioss pink1
    TA activated mitophagy by <t>PINK1/Parkin</t> signaling pathway. A The Western blotting detection of the protein expressions of LC3I and LC3II in HT22 cells treated with TA and Rap (20 μM). B The bar chart presents the rate of LC3II/LC3I, * p < 0.05, * p < 0.01 and *** p < 0.01 versus the control group, n = 3. C Representative images of stable GFP-LC3 U87 cells treated with or without TA, Magnification: 20 × , scale bar: 100 μm. D The bar chart presents the rate of cells with GFP-LC3 puncta formation in stable GFP-LC3 U87 cells, * p < 0.05 and *** p < 0.001 versus control group. E Representative images of stable stable RFP-GFP-LC3 U87 cells treated with or without TA, Baf (100 nM) or Rap (10 μM), Magnification: × 64, scale bar: 5 μm. F Representative images of the co-localization of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells with or without TA, CCCP or TA+AC220. G The bar chart indicates the rate of GFP/RFP, ** p < 0.05 and *** p < 0.001. H – K Line scan analysis showing the co-localization intensity (a.u.) of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells under different treatments. L Representative images of HT22 cells transiently transfecting Mito-QC plasmid and treated with or without TA, CCCP or TA+AC220. M Representative images of BV2 cells transiently transfecting Mito-QC and treated with or without TA, CCCP or TA+AC220. N The bar chart indicates the rate of RFP/GFP in HT22 ransiently transfecting Mito-QC cells. ** p < 0.01 and *** p < 0.001 versus the control group, n = 3. O The bar chart indicates the rate of RFP/GFP in BV2 ransiently transfecting Mito-QC cells. ** p <0.01 and *** p < 0.001 versus the control group, n = 3. P Western blot detection of Parkin, p-Parkin, and PINK1 protein expression in HT22 cells treated with or without TA. Q , R The bar chart presents the rate of p-Parkin/Parkin (n = 3), PINK1/GAPDH (n = 5). ** p < 0.01 and *** p < 0.001 versus the control group
    Pink1, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pink1/product/Bioss
    Average 94 stars, based on 1 article reviews
    pink1 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    High uric acid (HUA) induces mitophagy in hepatocytes . A , representative Western blot (WB) images of PINK1, LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 6, 12, 24, and 48 h). B – D , quantification of PINK1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). E , representative WB images of P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 3, 6, 9, and 12 h). F – M , quantification of P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). N , representative WB images of P62, P-PINK1 S228 , and PINK1 protein levels in the mitochondrial fraction of HepG2 cells following HUA treatment at various time points (0, 3, 6, and 12 h). O – Q , quantification of mitochondrial P62, P-PINK1, and PINK1 protein levels in HepG2 cells (n = 3). R , representative WB images of UB protein expression in primary mouse hepatocytes treated with or without HUA and MG132 (10 μM) for 9 h. S , RT–PCR analysis of P62, PINK1, Parkin, MFN1, MFN2, OPA1, DRP1, and VDAC1 mRNA expression in primary hepatocytes treated with HUA for different durations (0, 3, 6, and 9 h). T , RT–PCR analysis of PGC1A, NRF1, and TFAM mRNA expression in primary hepatocytes treated with HUA for different durations (0, 6, 12, and 24 h). U , representative confocal microscopy images showing LC3B ( red ) and TOM20 ( green ) colocalization in primary hepatocytes treated with HUA for 9 h. Nuclei were counterstained with DAPI ( blue ). The scale bar represents 20 μm. V , quantification of LC3B mean fluorescence intensity (n = 3). W , Pearson’s correlation coefficient between LC3B and TOM20 from experiments shown in U (n = 3). Individual data points represent each independent experiment. Data are expressed as mean ± SD. Statistical analysis was done using unpaired t test and one-way ANOVA, Tukey’s post hoc analysis. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 compared with the indicated groups. DAPI, 4',6-diamidino-2-phenylindole.

    Journal: The Journal of Biological Chemistry

    Article Title: Activation of mitophagy antagonizes high uric acid–induced hepatic lipid accumulation

    doi: 10.1016/j.jbc.2025.111054

    Figure Lengend Snippet: High uric acid (HUA) induces mitophagy in hepatocytes . A , representative Western blot (WB) images of PINK1, LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 6, 12, 24, and 48 h). B – D , quantification of PINK1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). E , representative WB images of P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B, and COXⅣ protein expression in primary mouse hepatocytes treated with HUA for different durations (0, 3, 6, 9, and 12 h). F – M , quantification of P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B-II/I, and COXⅣ protein levels in primary hepatocytes (n = 3). N , representative WB images of P62, P-PINK1 S228 , and PINK1 protein levels in the mitochondrial fraction of HepG2 cells following HUA treatment at various time points (0, 3, 6, and 12 h). O – Q , quantification of mitochondrial P62, P-PINK1, and PINK1 protein levels in HepG2 cells (n = 3). R , representative WB images of UB protein expression in primary mouse hepatocytes treated with or without HUA and MG132 (10 μM) for 9 h. S , RT–PCR analysis of P62, PINK1, Parkin, MFN1, MFN2, OPA1, DRP1, and VDAC1 mRNA expression in primary hepatocytes treated with HUA for different durations (0, 3, 6, and 9 h). T , RT–PCR analysis of PGC1A, NRF1, and TFAM mRNA expression in primary hepatocytes treated with HUA for different durations (0, 6, 12, and 24 h). U , representative confocal microscopy images showing LC3B ( red ) and TOM20 ( green ) colocalization in primary hepatocytes treated with HUA for 9 h. Nuclei were counterstained with DAPI ( blue ). The scale bar represents 20 μm. V , quantification of LC3B mean fluorescence intensity (n = 3). W , Pearson’s correlation coefficient between LC3B and TOM20 from experiments shown in U (n = 3). Individual data points represent each independent experiment. Data are expressed as mean ± SD. Statistical analysis was done using unpaired t test and one-way ANOVA, Tukey’s post hoc analysis. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 compared with the indicated groups. DAPI, 4',6-diamidino-2-phenylindole.

    Article Snippet: Antibodies for PINK1 (1:1000 dilution; catalog no.: 6946), P62 (1:1000 dilution; catalog no.: 5114), and F4/80 (1:200 dilution; catalog no.: 70076) were sourced from Cell Signaling Technology; P-Parkin S65 (1:1000 dilution; Ab315376 ) was from Abcam; P-PINK1 S228 (1:1000 dilution; AF7081) was from Affinity; TOM20 (1:200 dilution; catalog no.: 66777), Parkin (1:2000 dilution; catalog no.: 14060), ubiquitin (1:2000 dilution; catalog no.: 10201), and GAPDH (1:2000 dilution; catalog no.: 60004) from Proteintech, CD36 (1:1000dilution; NB400-144) from Novus Biologicals; and MFN2 (1:1000 dilution; A19678), LC3B (1:1000 dilution; A19665), and COXIV (1:1000 dilution; A11631) from Abclone.

    Techniques: Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Confocal Microscopy, Fluorescence

    High uric acid induces mitophagy in mouse liver . A , representative Wesetrn blot (WB) images showing the expression of mitophagy-related proteins (P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, and LC3B-II/I), mitochondrial fusion proteins MFN2 (mitofusin 2), and the mitochondrial marker COXIV in liver tissues from WT and Uox -KO mice. B – J , quantification of MFN2, P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B-II/I, and COXIV protein levels (n = 3–5). K , RT–PCR analysis of mitochondrial dynamics–related gene expression in liver tissues from WT and Uox -KO mice (n = 4–8). L , gene set enrichment analysis (GSEA) plot for the “mitochondrial outer membrane” Gene Ontology term. Individual data points on the graph represent data from each mouse. Data are presented as mean ± SD. Statistical analysis was done using unpaired t test and one-way ANOVA, Tukey’s post hoc analysis. ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 compared with the indicated groups.

    Journal: The Journal of Biological Chemistry

    Article Title: Activation of mitophagy antagonizes high uric acid–induced hepatic lipid accumulation

    doi: 10.1016/j.jbc.2025.111054

    Figure Lengend Snippet: High uric acid induces mitophagy in mouse liver . A , representative Wesetrn blot (WB) images showing the expression of mitophagy-related proteins (P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, and LC3B-II/I), mitochondrial fusion proteins MFN2 (mitofusin 2), and the mitochondrial marker COXIV in liver tissues from WT and Uox -KO mice. B – J , quantification of MFN2, P62, P-PINK1 S228 , PINK1, P-Parkin S65 , Parkin, VDAC1, LC3B-II/I, and COXIV protein levels (n = 3–5). K , RT–PCR analysis of mitochondrial dynamics–related gene expression in liver tissues from WT and Uox -KO mice (n = 4–8). L , gene set enrichment analysis (GSEA) plot for the “mitochondrial outer membrane” Gene Ontology term. Individual data points on the graph represent data from each mouse. Data are presented as mean ± SD. Statistical analysis was done using unpaired t test and one-way ANOVA, Tukey’s post hoc analysis. ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 compared with the indicated groups.

    Article Snippet: Antibodies for PINK1 (1:1000 dilution; catalog no.: 6946), P62 (1:1000 dilution; catalog no.: 5114), and F4/80 (1:200 dilution; catalog no.: 70076) were sourced from Cell Signaling Technology; P-Parkin S65 (1:1000 dilution; Ab315376 ) was from Abcam; P-PINK1 S228 (1:1000 dilution; AF7081) was from Affinity; TOM20 (1:200 dilution; catalog no.: 66777), Parkin (1:2000 dilution; catalog no.: 14060), ubiquitin (1:2000 dilution; catalog no.: 10201), and GAPDH (1:2000 dilution; catalog no.: 60004) from Proteintech, CD36 (1:1000dilution; NB400-144) from Novus Biologicals; and MFN2 (1:1000 dilution; A19678), LC3B (1:1000 dilution; A19665), and COXIV (1:1000 dilution; A11631) from Abclone.

    Techniques: Expressing, Marker, Reverse Transcription Polymerase Chain Reaction, Gene Expression, Membrane

    Schematic diagram of activation of mitophagy antagonizes high uric acid–induced hepatic lipid accumulation (created with Figdraw.com ) . High uric acid upregulates CD36, promoting hepatic fat accumulation. Meanwhile, it activates mitophagy via the PINK1–Parkin pathway, which antagonizes this lipid accumulation.

    Journal: The Journal of Biological Chemistry

    Article Title: Activation of mitophagy antagonizes high uric acid–induced hepatic lipid accumulation

    doi: 10.1016/j.jbc.2025.111054

    Figure Lengend Snippet: Schematic diagram of activation of mitophagy antagonizes high uric acid–induced hepatic lipid accumulation (created with Figdraw.com ) . High uric acid upregulates CD36, promoting hepatic fat accumulation. Meanwhile, it activates mitophagy via the PINK1–Parkin pathway, which antagonizes this lipid accumulation.

    Article Snippet: Antibodies for PINK1 (1:1000 dilution; catalog no.: 6946), P62 (1:1000 dilution; catalog no.: 5114), and F4/80 (1:200 dilution; catalog no.: 70076) were sourced from Cell Signaling Technology; P-Parkin S65 (1:1000 dilution; Ab315376 ) was from Abcam; P-PINK1 S228 (1:1000 dilution; AF7081) was from Affinity; TOM20 (1:200 dilution; catalog no.: 66777), Parkin (1:2000 dilution; catalog no.: 14060), ubiquitin (1:2000 dilution; catalog no.: 10201), and GAPDH (1:2000 dilution; catalog no.: 60004) from Proteintech, CD36 (1:1000dilution; NB400-144) from Novus Biologicals; and MFN2 (1:1000 dilution; A19678), LC3B (1:1000 dilution; A19665), and COXIV (1:1000 dilution; A11631) from Abclone.

    Techniques: Activation Assay

    TA activated mitophagy by PINK1/Parkin signaling pathway. A The Western blotting detection of the protein expressions of LC3I and LC3II in HT22 cells treated with TA and Rap (20 μM). B The bar chart presents the rate of LC3II/LC3I, * p < 0.05, * p < 0.01 and *** p < 0.01 versus the control group, n = 3. C Representative images of stable GFP-LC3 U87 cells treated with or without TA, Magnification: 20 × , scale bar: 100 μm. D The bar chart presents the rate of cells with GFP-LC3 puncta formation in stable GFP-LC3 U87 cells, * p < 0.05 and *** p < 0.001 versus control group. E Representative images of stable stable RFP-GFP-LC3 U87 cells treated with or without TA, Baf (100 nM) or Rap (10 μM), Magnification: × 64, scale bar: 5 μm. F Representative images of the co-localization of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells with or without TA, CCCP or TA+AC220. G The bar chart indicates the rate of GFP/RFP, ** p < 0.05 and *** p < 0.001. H – K Line scan analysis showing the co-localization intensity (a.u.) of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells under different treatments. L Representative images of HT22 cells transiently transfecting Mito-QC plasmid and treated with or without TA, CCCP or TA+AC220. M Representative images of BV2 cells transiently transfecting Mito-QC and treated with or without TA, CCCP or TA+AC220. N The bar chart indicates the rate of RFP/GFP in HT22 ransiently transfecting Mito-QC cells. ** p < 0.01 and *** p < 0.001 versus the control group, n = 3. O The bar chart indicates the rate of RFP/GFP in BV2 ransiently transfecting Mito-QC cells. ** p <0.01 and *** p < 0.001 versus the control group, n = 3. P Western blot detection of Parkin, p-Parkin, and PINK1 protein expression in HT22 cells treated with or without TA. Q , R The bar chart presents the rate of p-Parkin/Parkin (n = 3), PINK1/GAPDH (n = 5). ** p < 0.01 and *** p < 0.001 versus the control group

    Journal: Chinese Medicine

    Article Title: Thonningianin A derived from Penthorum chinense Pursh alleviates cerebral ischemia/reperfusion-mediated apoptosis and pyroptosis through the activation of PINK1/Parkin-dependent mitophagy

    doi: 10.1186/s13020-025-01247-2

    Figure Lengend Snippet: TA activated mitophagy by PINK1/Parkin signaling pathway. A The Western blotting detection of the protein expressions of LC3I and LC3II in HT22 cells treated with TA and Rap (20 μM). B The bar chart presents the rate of LC3II/LC3I, * p < 0.05, * p < 0.01 and *** p < 0.01 versus the control group, n = 3. C Representative images of stable GFP-LC3 U87 cells treated with or without TA, Magnification: 20 × , scale bar: 100 μm. D The bar chart presents the rate of cells with GFP-LC3 puncta formation in stable GFP-LC3 U87 cells, * p < 0.05 and *** p < 0.001 versus control group. E Representative images of stable stable RFP-GFP-LC3 U87 cells treated with or without TA, Baf (100 nM) or Rap (10 μM), Magnification: × 64, scale bar: 5 μm. F Representative images of the co-localization of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells with or without TA, CCCP or TA+AC220. G The bar chart indicates the rate of GFP/RFP, ** p < 0.05 and *** p < 0.001. H – K Line scan analysis showing the co-localization intensity (a.u.) of GFP-LC3 and MitoTracker in stable GFP-LC3 U87 cells under different treatments. L Representative images of HT22 cells transiently transfecting Mito-QC plasmid and treated with or without TA, CCCP or TA+AC220. M Representative images of BV2 cells transiently transfecting Mito-QC and treated with or without TA, CCCP or TA+AC220. N The bar chart indicates the rate of RFP/GFP in HT22 ransiently transfecting Mito-QC cells. ** p < 0.01 and *** p < 0.001 versus the control group, n = 3. O The bar chart indicates the rate of RFP/GFP in BV2 ransiently transfecting Mito-QC cells. ** p <0.01 and *** p < 0.001 versus the control group, n = 3. P Western blot detection of Parkin, p-Parkin, and PINK1 protein expression in HT22 cells treated with or without TA. Q , R The bar chart presents the rate of p-Parkin/Parkin (n = 3), PINK1/GAPDH (n = 5). ** p < 0.01 and *** p < 0.001 versus the control group

    Article Snippet: Antibodies against GSDMD (20770-1-Ap), Caspase-3 (25158-1-Ap), Caspase-9 (10380-1-Ap), NQO1 (11451-1-Ap), GCLC (12601-1-Ap), LC3 (14600-1-Ap), PINK1 (23274-1-Ap), Parkin (14060-1-Ap), Antibody Caspase-1 (22,915-1-ap), GAPDH (6004-1-Ig), β-actin (66009-1-Ig), CoraLite ® Plus 488 goat anti-rabbit IgG (RGAR002), CoraLite ® Plus 594-Goat Anti-Mouse IgG (RGAR002), were purchased from Proteintech Group, Inc (Wuhan, China).

    Techniques: Western Blot, Control, Plasmid Preparation, Expressing

    TA ameliorated neurological injury in MCAO/R Rats. A Representative immunofluorescence staining images of LC3 and NLRP3 in hippocampus of the brain in MCAO/R rats, × 10, scale bar: 100 μm. B The bar chart presents the counts of LC3 + cells, NLRP3 + /cells, and LC3 + /NLRP3 + co-localized cells. C – F The Western blotting detection of the protein expressions of LC3, PINK1, p-Parkin and NLRP3 in rat brain tissues across experimental groups. G – J The bar chart presents the ratios of LC3-II/I, PINK1/β-actin, p-Parkin/ Parkin, NLRP3/β-actin. * p < 0.05, ** p < 0.01 and *** p < 0.001versus the alone MCAO/R group, n = 3. K – N The Western blotting detection of the protein expressions of ASC, Caspase-1, IL-18 in rat brain tissues across experimental groups. O – R The bar chart presents the ratios of ASC/β-actin, Cleaved-Caspase-1/Pro- Caspase-1, Cleaved-IL-18/β-actin. * p < 0.05, ** p < 0.01 and *** p < 0.001versus the alone MCAO/R group, n = 3. S The Western blotting detection of the protein expression of IL-1β. T The bar chart presents the rate of Cleaved-IL-18/GAPDH. * p < 0.05 and ** p < 0.01 versus the alone MCAO/R group, n = 3. S The Western blotting detection of the protein expression of IL-1β. T The bar chart presents the rate of Cleaved-IL-1β/GAPDH. * p < 0.05 and ** p < 0.01 versus the alone MCAO/R group, n = 3. U The Western blotting detection of the protein expression of BAX and Bcl2. V The bar chart presents the rate of BAX/Bcl2. *** p < 0.001 versus the alone MCAO/R group, n = 3

    Journal: Chinese Medicine

    Article Title: Thonningianin A derived from Penthorum chinense Pursh alleviates cerebral ischemia/reperfusion-mediated apoptosis and pyroptosis through the activation of PINK1/Parkin-dependent mitophagy

    doi: 10.1186/s13020-025-01247-2

    Figure Lengend Snippet: TA ameliorated neurological injury in MCAO/R Rats. A Representative immunofluorescence staining images of LC3 and NLRP3 in hippocampus of the brain in MCAO/R rats, × 10, scale bar: 100 μm. B The bar chart presents the counts of LC3 + cells, NLRP3 + /cells, and LC3 + /NLRP3 + co-localized cells. C – F The Western blotting detection of the protein expressions of LC3, PINK1, p-Parkin and NLRP3 in rat brain tissues across experimental groups. G – J The bar chart presents the ratios of LC3-II/I, PINK1/β-actin, p-Parkin/ Parkin, NLRP3/β-actin. * p < 0.05, ** p < 0.01 and *** p < 0.001versus the alone MCAO/R group, n = 3. K – N The Western blotting detection of the protein expressions of ASC, Caspase-1, IL-18 in rat brain tissues across experimental groups. O – R The bar chart presents the ratios of ASC/β-actin, Cleaved-Caspase-1/Pro- Caspase-1, Cleaved-IL-18/β-actin. * p < 0.05, ** p < 0.01 and *** p < 0.001versus the alone MCAO/R group, n = 3. S The Western blotting detection of the protein expression of IL-1β. T The bar chart presents the rate of Cleaved-IL-18/GAPDH. * p < 0.05 and ** p < 0.01 versus the alone MCAO/R group, n = 3. S The Western blotting detection of the protein expression of IL-1β. T The bar chart presents the rate of Cleaved-IL-1β/GAPDH. * p < 0.05 and ** p < 0.01 versus the alone MCAO/R group, n = 3. U The Western blotting detection of the protein expression of BAX and Bcl2. V The bar chart presents the rate of BAX/Bcl2. *** p < 0.001 versus the alone MCAO/R group, n = 3

    Article Snippet: Antibodies against GSDMD (20770-1-Ap), Caspase-3 (25158-1-Ap), Caspase-9 (10380-1-Ap), NQO1 (11451-1-Ap), GCLC (12601-1-Ap), LC3 (14600-1-Ap), PINK1 (23274-1-Ap), Parkin (14060-1-Ap), Antibody Caspase-1 (22,915-1-ap), GAPDH (6004-1-Ig), β-actin (66009-1-Ig), CoraLite ® Plus 488 goat anti-rabbit IgG (RGAR002), CoraLite ® Plus 594-Goat Anti-Mouse IgG (RGAR002), were purchased from Proteintech Group, Inc (Wuhan, China).

    Techniques: Immunofluorescence, Staining, Western Blot, Expressing