pikachurin (Proteintech)
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pikachurin
Pikachurin, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pikachurin/Pikachurin+Antibody/bio_rxiv__64898__2026__03__15__710015-170-56-58
Average 93 stars, based on 7 article reviews
Pikachurin, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pikachurin/Pikachurin+Antibody/bio_rxiv__64898__2026__03__15__710015-170-56-58
Average 93 stars, based on 7 article reviews
pikachurin - by Bioz Stars,
2026-11
93/100 stars
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1) Product Images from "Dyrk1a gene dosage controls bipolar cell development and retinal connectivity"
Article Title: Dyrk1a gene dosage controls bipolar cell development and retinal connectivity
Journal: bioRxiv
doi: 10.64898/2026.03.15.710015
Figure Legend Snippet: (A) Schematic representation of electroretinogram (ERG) components. An initial negative deflection (a-wave) is observed upon photoreceptor stimulation (arrow) that is followed by a subsequent positive deflection (b-wave) that corresponds to bipolar cell activity. ERG waveform quantification involves measuring the amplitude (μV) and latency (ms) of the a- and b-waves to increasing stimulus intensities (cd.s/m 2 ) and thus provides an evaluation of retinal function. (B) Representative dark-adapted scotopic ERG waveforms recorded from Lhx2-Cre ( n = 3) and Lhx2-Cre:Dyrk1a +/f ( n = 3) animals (6 – 8 weeks) in response to a single flash stimulus (log 1.48 cd.s/m 2 ). Comparable a-wave responses are observed for both groups while b-wave amplitudes are reduced in heterozygous animals. Thick lines represent genotype-mean responses while thin lines represent individual mice. (C – D) Quantifcation of photoreceptor amplitude (C) and latency (D) responses in Lhx2-Cre ( n = 3) and Lhx2-Cre:Dyrk1a +/f ( n = 3) animals (6 – 8 weeks) in repsonse to a series of increasing scotopic flash stimuli (log cd.s/m 2 ). No significant differences are observed between the groups. (E – F) Quantification of bipolar cell amplitude (E) and latency (F) responses in Lhx2-Cre ( n = 3) and Lhx2-Cre:Dyrk1a +/f ( n = 3) animal (6 – 8 weeks) towards increasing scotopic flash stimuli (log cd.s/m 2 ). Heterozygous animals have a significant reduction in bipolar cell amplitude response at higher stimulus intensities (E) albeit with similar latency (F). (G) Representative light-adapted photopic ERG waveforms recorded from Lhx2-Cre ( n = 3) and Lhx2-Cre:Dyrk1a +/f ( n = 3) animals (6 – 8 weeks) in response to a single UV flash stimulus (log 1.00 cd.s/m 2 ). Comparable a-wave responses are observed for both groups while b-wave amplitudes are reduced in heterozygous animals. Thick lines represent genotype-mean responses while thin lines refer to individual mice. (H) Quantification of bipolar cell amplitude response in Lhx2-Cre ( n = 3) and Lhx2-Cre:Dyrk1a +/f ( n = 3) animal (6 – 8 weeks) in response to an increasing photopic UV stimulus intensity series (log cd.s/m 2 ). Heterozygous animals have a significant reduction in bipolar cell amplitude response at higher stimulus intensities. (I) Representative light-adapted photopic ERG waveforms recorded from Lhx2-Cre ( n = 3) and Lhx2-Cre:Dyrk1a +/f ( n = 3) animals (6 – 8 weeks) in response to a single green flash stimulus (log 1.48 cd.s/m 2 ). Comparable a-wave responses are observed for both groups while b-wave amplitudes are reduced in heterozygous animals. Thick lines represent genotype-mean responses while thin lines refer to individual mice. (J) Quantification of bipolar cell amplitude response in Lhx2-Cre ( n = 3) and Lhx2-Cre:Dyrk1a +/f ( n = 3) animal (6 – 8 weeks) in response to a photopic green stimulus intensity series (log cd.s/m 2 ). Heterozygous animals have a significant reduction in bipolar cell amplitude response at higher stimulus intensities. (K – R) Representative immunohistochemical staining of coronal eye sections taken from Lhx2-Cre and Lhx2-Cre:Dyrk1a +/f adult mice (6 – 8 weeks) across four designated dorsoventral quadrants (D1, D2, V3 and V4) spanning the entire retina relative to the optic nerve head. OPL ribbon synapse assemblies appear comparable in both groups (K – R, arrowheads) and were composed of dense photoreceptor terminals (vGlut1 + ) that overlapped with well organised bipolar cell dendritic processes (PKCα + ). Additionally, the linear alignment of all ribbon junctions (Pikachurin + ) indicated intact photoreceptor and bipolar cell synapses. All data represents the mean ± SEM. Statistical differences were calculated using a two-way ANOVA followed by a Sidak’s multiple comparison test. p-values are denoted as follows: **p≤ 0.01, ***p≤ 0.001 and ****p≤ 0.0001. Scale bar: (K – R) 5 µm. Abbreviations: D, dorsal; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; V, ventral.
Techniques Used: Activity Assay, Immunohistochemical staining, Staining, Comparison
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Activity Assay:Article Title: Dyrk1a gene dosage controls bipolar cell development and retinal connectivity Article Snippet: Blocking Reagent (Vector Labs) was used in experiments involving monoclonal primary antibodies.Blocking Reagent (Vector Labs) was used in experiments involving monoclonal primary antibodies.. The following primary antibodies and dilutions were used in this study: Calbindin (1:300, Sigma-Aldrich, #C9848); Calretinin (1:1000, Swant, #CG1); cleaved Caspase3 (Asp175) (1:500, Cell Signalling, #9661S); chAT (1:100, Millipore, #AB144P); Chx10 (1:50, SCBT, #SC365519); Dyrk1a (1:100, Novus, #NBP1-84032); Isl1/2 (recognises both Isl1 and Isl2 proteins) (1:500; DSHB, #39.4D5); M-opsin (1:500, Article Title: Novel Grm6 Variant in a no b-wave (nob) Mouse Model: Phenotype Characterization and Gene Therapy Article Snippet: Hematoxylin and eosin staining and IF staining of retinal cryosections were conducted using previously described methods.Hematoxylin and eosin staining and IF staining of retinal cryosections were conducted using previously described methods.. For the IF, the following antibodies were used: danti-PKCA (SC-8393; Santa Cruz, Dallas, TX, USA), anti-mGluR6 (from Dr. Melina A. Agosto), anti-TRPM1 (from Dr. Melina A. Agosto), anti-CtBP2 (10346-1-AP; Proteintech, Rosemont, IL, USA), anti-GoA (MAB-3073; Millipore–Sigma, Burlington, MA, USA), anti-PSD95 (AB18258; Abcam, Cambridge, MA, USA), Immunohistochemical staining:Article Title: Dyrk1a gene dosage controls bipolar cell development and retinal connectivity Article Snippet: Blocking Reagent (Vector Labs) was used in experiments involving monoclonal primary antibodies.Blocking Reagent (Vector Labs) was used in experiments involving monoclonal primary antibodies.. The following primary antibodies and dilutions were used in this study: Calbindin (1:300, Sigma-Aldrich, #C9848); Calretinin (1:1000, Swant, #CG1); cleaved Caspase3 (Asp175) (1:500, Cell Signalling, #9661S); chAT (1:100, Millipore, #AB144P); Chx10 (1:50, SCBT, #SC365519); Dyrk1a (1:100, Novus, #NBP1-84032); Isl1/2 (recognises both Isl1 and Isl2 proteins) (1:500; DSHB, #39.4D5); M-opsin (1:500, Article Title: Novel Grm6 Variant in a no b-wave (nob) Mouse Model: Phenotype Characterization and Gene Therapy Article Snippet: Hematoxylin and eosin staining and IF staining of retinal cryosections were conducted using previously described methods.Hematoxylin and eosin staining and IF staining of retinal cryosections were conducted using previously described methods.. For the IF, the following antibodies were used: danti-PKCA (SC-8393; Santa Cruz, Dallas, TX, USA), anti-mGluR6 (from Dr. Melina A. Agosto), anti-TRPM1 (from Dr. Melina A. Agosto), anti-CtBP2 (10346-1-AP; Proteintech, Rosemont, IL, USA), anti-GoA (MAB-3073; Millipore–Sigma, Burlington, MA, USA), anti-PSD95 (AB18258; Abcam, Cambridge, MA, USA), Staining:Article Title: Dyrk1a gene dosage controls bipolar cell development and retinal connectivity Article Snippet: Blocking Reagent (Vector Labs) was used in experiments involving monoclonal primary antibodies.Blocking Reagent (Vector Labs) was used in experiments involving monoclonal primary antibodies.. The following primary antibodies and dilutions were used in this study: Calbindin (1:300, Sigma-Aldrich, #C9848); Calretinin (1:1000, Swant, #CG1); cleaved Caspase3 (Asp175) (1:500, Cell Signalling, #9661S); chAT (1:100, Millipore, #AB144P); Chx10 (1:50, SCBT, #SC365519); Dyrk1a (1:100, Novus, #NBP1-84032); Isl1/2 (recognises both Isl1 and Isl2 proteins) (1:500; DSHB, #39.4D5); M-opsin (1:500, Article Title: Novel Grm6 Variant in a no b-wave (nob) Mouse Model: Phenotype Characterization and Gene Therapy Article Snippet: Hematoxylin and eosin staining and IF staining of retinal cryosections were conducted using previously described methods.Hematoxylin and eosin staining and IF staining of retinal cryosections were conducted using previously described methods.. For the IF, the following antibodies were used: danti-PKCA (SC-8393; Santa Cruz, Dallas, TX, USA), anti-mGluR6 (from Dr. Melina A. Agosto), anti-TRPM1 (from Dr. Melina A. Agosto), anti-CtBP2 (10346-1-AP; Proteintech, Rosemont, IL, USA), anti-GoA (MAB-3073; Millipore–Sigma, Burlington, MA, USA), anti-PSD95 (AB18258; Abcam, Cambridge, MA, USA), Comparison:Article Title: Dyrk1a gene dosage controls bipolar cell development and retinal connectivity Article Snippet: Blocking Reagent (Vector Labs) was used in experiments involving monoclonal primary antibodies.Blocking Reagent (Vector Labs) was used in experiments involving monoclonal primary antibodies.. The following primary antibodies and dilutions were used in this study: Calbindin (1:300, Sigma-Aldrich, #C9848); Calretinin (1:1000, Swant, #CG1); cleaved Caspase3 (Asp175) (1:500, Cell Signalling, #9661S); chAT (1:100, Millipore, #AB144P); Chx10 (1:50, SCBT, #SC365519); Dyrk1a (1:100, Novus, #NBP1-84032); Isl1/2 (recognises both Isl1 and Isl2 proteins) (1:500; DSHB, #39.4D5); M-opsin (1:500, Article Title: Novel Grm6 Variant in a no b-wave (nob) Mouse Model: Phenotype Characterization and Gene Therapy Article Snippet: Hematoxylin and eosin staining and IF staining of retinal cryosections were conducted using previously described methods.Hematoxylin and eosin staining and IF staining of retinal cryosections were conducted using previously described methods.. For the IF, the following antibodies were used: danti-PKCA (SC-8393; Santa Cruz, Dallas, TX, USA), anti-mGluR6 (from Dr. Melina A. Agosto), anti-TRPM1 (from Dr. Melina A. Agosto), anti-CtBP2 (10346-1-AP; Proteintech, Rosemont, IL, USA), anti-GoA (MAB-3073; Millipore–Sigma, Burlington, MA, USA), anti-PSD95 (AB18258; Abcam, Cambridge, MA, USA), Western Blot:Article Title: Dyrk1a gene dosage controls bipolar cell development and retinal connectivity Article Snippet: Blocking Reagent (Vector Labs) was used in experiments involving monoclonal primary antibodies.Blocking Reagent (Vector Labs) was used in experiments involving monoclonal primary antibodies.. The following primary antibodies and dilutions were used in this study: Calbindin (1:300, Sigma-Aldrich, #C9848); Calretinin (1:1000, Swant, #CG1); cleaved Caspase3 (Asp175) (1:500, Cell Signalling, #9661S); chAT (1:100, Millipore, #AB144P); Chx10 (1:50, SCBT, #SC365519); Dyrk1a (1:100, Novus, #NBP1-84032); Isl1/2 (recognises both Isl1 and Isl2 proteins) (1:500; DSHB, #39.4D5); M-opsin (1:500, Article Title: Novel Grm6 Variant in a no b-wave (nob) Mouse Model: Phenotype Characterization and Gene Therapy Article Snippet: Hematoxylin and eosin staining and IF staining of retinal cryosections were conducted using previously described methods.Hematoxylin and eosin staining and IF staining of retinal cryosections were conducted using previously described methods.. For the IF, the following antibodies were used: danti-PKCA (SC-8393; Santa Cruz, Dallas, TX, USA), anti-mGluR6 (from Dr. Melina A. Agosto), anti-TRPM1 (from Dr. Melina A. Agosto), anti-CtBP2 (10346-1-AP; Proteintech, Rosemont, IL, USA), anti-GoA (MAB-3073; Millipore–Sigma, Burlington, MA, USA), anti-PSD95 (AB18258; Abcam, Cambridge, MA, USA), |
