phenotype  (Medicago)

Journal: Advanced Science

Article Title: Alternating Shear Force of Respiration Regulates Cell Interactions of Fibroblasts and Macrophages to Promote Soft Tissue Integration of Chest Wall Polyetheretherketone Implants

doi: 10.1002/advs.77118

Figure Lengend Snippet: Mechanical force modulates macrophage M2 polarization on PEEK surfaces. (A) The expression levels of mechanical perception (PIEZO1, YAP1) and polarization‐related proteins (iNOS, CD206, STAT6/p‐STAT6, NF‐κB/p‐NF‐κB) in dynamically cultured RAW264.7, determined by Western blot. (B) Gene expression in RAW264.7 were cultured under dynamic and static conditions for 12 h. (C) SEM images of RAW264.7 under static and dynamic culture conditions (scale bar: 10 µm). (D) Confocal fluorescence images of CD206, PIEZO1, iNOS, IL10, YAP1 and ITGB1 in macrophages under dynamic and static conditions (scale bar: 100 µm). The average fluorescence intensity of (E) CD206, IL‐10, (F) PIEZO1, YAP1, and (G) iNOS, ITGB1 were statistically analyzed. Shown are mean values ± SD (n = 3 independent experiments, each with 3 technical replicates), * P < 0.05, ** P < 0.01, and *** P < 0.001, ns, no significant difference. 2‐way ANOVA was used in (B) and (E–G).

Article Snippet: To induce M2 phenotype, M0 macrophages were stimulated with 10 ng/mL IL‐10 (HY‐P70517, MedChemExpress LLC., USA) and 20 ng/mL IL‐4 (HY‐ P70653 , MedChemExpress LLC., USA)

Techniques: Expressing, Cell Culture, Western Blot, Gene Expression, Fluorescence

Journal: Horticulture Research

Article Title: The CsBES1-14-CsCOR413 module mediated by brassinolide positively regulates cold resistance in tea plant

doi: 10.1093/hr/uhag098

Figure Lengend Snippet: The impact of CsBES1-14 silencing on the cold tolerance of tea plant is significant. (a) Examination of the phenotypic traits related to the cold tolerance in various tea plant varieties. Note: normal temperature control; Growth of tea plant treated at 4°C. (b) Expression profiling of CsSK1 , CsTCH4 , and CsCYCD3 , components of the BR signaling pathway. (c) Transcript abundance of CsICE1 , CsCBF1 , and CsCOR47 in cold-stressed tea plant. (d) The analysis of electrical conductivity for the tea leaves of WT, TRV: EV, and TRV: CsBES1-14 was conducted at different temperature ranges. TRV: EV: Empty Vector; TRV: CsBES1-14 : Plants in which the CsBES1-14 was silenced.

Article Snippet: After 2–3 days of incubation at 30°C, phenotypic observations (growth and color) were made for experimental and control sets (Yeasen, Shanghai, China; Cat. 10923ES05).

Techniques: Control, Expressing, Plasmid Preparation

Journal: Horticulture Research

Article Title: The CsBES1-14-CsCOR413 module mediated by brassinolide positively regulates cold resistance in tea plant

doi: 10.1093/hr/uhag098

Figure Lengend Snippet: Expression pattern of CsCOR413 and analysis of cold resistance in tea plant following its silencing. (a) Transcriptional levels of CsCOR413 following cold treatment of tea plant after BL application. (b) Transcriptional levels of CsCOR413 following cold treatment of tea plant after BRZ application. (c) Analysis of CsCOR413 expression in silenced plants. (d) Analysis of leaf relative conductivity of different plants under cold stress. (e) Phenotypic analysis of plants silenced by CsCOR413 under low-temperature stress.

Article Snippet: After 2–3 days of incubation at 30°C, phenotypic observations (growth and color) were made for experimental and control sets (Yeasen, Shanghai, China; Cat. 10923ES05).

Techniques: Expressing