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phelper  (TaKaRa)


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    Structured Review

    TaKaRa phelper
    Phelper, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phelper/AAVpro+Helper+Free+System/pmc13099357-278-29-32
    Average 94 stars, based on 53 article reviews
    phelper - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: IRF8 and FOXM1 Regulation in COPD Progression: Impacts on Inflammation and Senescence
    Article Snippet: .. The The packaging system included pHelper (Takara, 6230) and the AAV5 serotype plasmid pRC5 (Fenghui Biotechnology, ZT336). .. Transfection was carried out using Lipofectamine 3000 (Invitrogen, L3000015).

    Article Title: NR4A3 alleviates diabetic neuropathy via GLS2-mediated mitochondrial repair and Schwann cell differentiation
    Article Snippet: .. Transfection was performed using Lipofectamine 3000 (Invitrogen, USA) with 43.4 μL reagent and 56 μL P3000 enhancer per dish, along with 7 μg of shuttle plasmid, 14 μg of pHelper (Cat# 6230, Takara, China), and 7 μg of serotype plasmid pRC9 (Cat# BR227, FengHuiBio, China). ..

    Article Title: IRF8 and FOXM1 Regulation in COPD Progression: Impacts on Inflammation and Senescence.
    Article Snippet: .. The The packaging system included pHelper (Takara, 6230) and the AAV5 serotype plasmid pRC5 (Fenghui Biotechnology, ..

    Article Title: Role of HAUS7 as a DOCK3 binding partner in facilitating axon regeneration
    Article Snippet: .. Briefly, subconfluent HEK293 cells were transiently transfected with the vector plasmid and two helper plasmids, pRC2-mi342 and pHelper (6652; Takara), using polyethyleneimine MAX (24765, Polysciences). ..

    Bioprocessing:

    Article Title: Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo
    Article Snippet: .. AAV-DJ-syn-jGCaMP8m-WPRE vector was generated using pGP-AAV-syn-jGCaMP8m-WPRE (Addgene, 162375), pHelper (AAVpro Helper-free system, Takara), pAAV-DJ (Cell Biolabs) and the AAVpro 293T cell line (632273, Takara) following the manufacturers’ instructions. ..

    Article Title: AAV-mediated BDNF and GAS6 muscle delivery delays disease onset in SOD1 G93A ALS mice.
    Article Snippet: .. When reaching 3 million/mL concentration, VPCs were transfected with recombinant pAAV, pHelper (Takara Bio #6234), and pUCmini-iCap-PHP.eB [38] using the AAV-Max transfection kit. ..

    Generated:

    Article Title: Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo
    Article Snippet: .. AAV-DJ-syn-jGCaMP8m-WPRE vector was generated using pGP-AAV-syn-jGCaMP8m-WPRE (Addgene, 162375), pHelper (AAVpro Helper-free system, Takara), pAAV-DJ (Cell Biolabs) and the AAVpro 293T cell line (632273, Takara) following the manufacturers’ instructions. ..

    Transfection:

    Article Title: NR4A3 alleviates diabetic neuropathy via GLS2-mediated mitochondrial repair and Schwann cell differentiation
    Article Snippet: .. Transfection was performed using Lipofectamine 3000 (Invitrogen, USA) with 43.4 μL reagent and 56 μL P3000 enhancer per dish, along with 7 μg of shuttle plasmid, 14 μg of pHelper (Cat# 6230, Takara, China), and 7 μg of serotype plasmid pRC9 (Cat# BR227, FengHuiBio, China). ..

    Article Title: AAV-mediated BDNF and GAS6 muscle delivery delays disease onset in SOD1 G93A ALS mice.
    Article Snippet: .. When reaching 3 million/mL concentration, VPCs were transfected with recombinant pAAV, pHelper (Takara Bio #6234), and pUCmini-iCap-PHP.eB [38] using the AAV-Max transfection kit. ..

    Article Title: Role of HAUS7 as a DOCK3 binding partner in facilitating axon regeneration
    Article Snippet: .. Briefly, subconfluent HEK293 cells were transiently transfected with the vector plasmid and two helper plasmids, pRC2-mi342 and pHelper (6652; Takara), using polyethyleneimine MAX (24765, Polysciences). ..

    Concentration Assay:

    Article Title: AAV-mediated BDNF and GAS6 muscle delivery delays disease onset in SOD1 G93A ALS mice.
    Article Snippet: .. When reaching 3 million/mL concentration, VPCs were transfected with recombinant pAAV, pHelper (Takara Bio #6234), and pUCmini-iCap-PHP.eB [38] using the AAV-Max transfection kit. ..

    Recombinant:

    Article Title: AAV-mediated BDNF and GAS6 muscle delivery delays disease onset in SOD1 G93A ALS mice.
    Article Snippet: .. When reaching 3 million/mL concentration, VPCs were transfected with recombinant pAAV, pHelper (Takara Bio #6234), and pUCmini-iCap-PHP.eB [38] using the AAV-Max transfection kit. ..



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