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imageswere takenwith nikon eclipse ts2 inverted phase contrastmicroscope  (Nikon)


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    Nikon imageswere takenwith nikon eclipse ts2 inverted phase contrastmicroscope
    Imageswere Takenwith Nikon Eclipse Ts2 Inverted Phase Contrastmicroscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 57094 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 1. Establishment of BETi-resistant cells from NCI-H1975 cells. A, A schematic diagram of the establishment of ABBV-075–resistant variants. B, The IC50 values of ABBV- 075 in the parental cells and the resistant variants were determined by SRB assays. IC50 refers to the concentrations of ABBV-075 that caused 50% inhibition of cell proliferation after 72-hour treatment. C, Cell morphology of parental cells and resistant variants (RMIX, R1, R5, R6, and R9) was imaged under an Olympus <t>CKX53</t> phase contrast microscope using a 10objectivelens.Bars,100 mm. D, IC50 values of four BETis including ABBV-075, (þ)-JQ1, OTX015, and N2817 in parental cells and resistant variants (RMIX, R1, R5, R6, and R9) were detected by SRB assays after 72-hour treatments. E, Growth rates of indicated cells measured during 1–7-day incubation. Cell viability on each day was detected using CellTiter-Glo. Relative OD values were obtained by comparing the OD values on days 2–7 to that on day 1 and OD values of day 1 were normalized as one. Data from three independent experiments, if applicable, were expressed as mean SD (error bar); , P < 0.05.
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    Image Search Results


    Figure 1. Establishment of BETi-resistant cells from NCI-H1975 cells. A, A schematic diagram of the establishment of ABBV-075–resistant variants. B, The IC50 values of ABBV- 075 in the parental cells and the resistant variants were determined by SRB assays. IC50 refers to the concentrations of ABBV-075 that caused 50% inhibition of cell proliferation after 72-hour treatment. C, Cell morphology of parental cells and resistant variants (RMIX, R1, R5, R6, and R9) was imaged under an Olympus CKX53 phase contrast microscope using a 10objectivelens.Bars,100 mm. D, IC50 values of four BETis including ABBV-075, (þ)-JQ1, OTX015, and N2817 in parental cells and resistant variants (RMIX, R1, R5, R6, and R9) were detected by SRB assays after 72-hour treatments. E, Growth rates of indicated cells measured during 1–7-day incubation. Cell viability on each day was detected using CellTiter-Glo. Relative OD values were obtained by comparing the OD values on days 2–7 to that on day 1 and OD values of day 1 were normalized as one. Data from three independent experiments, if applicable, were expressed as mean SD (error bar); , P < 0.05.

    Journal: Molecular Cancer Research

    Article Title: Loss of VOPP1 Contributes to BET Inhibitor Acquired Resistance in Non–Small Cell Lung Cancer Cells

    doi: 10.1158/1541-7786.mcr-21-1000

    Figure Lengend Snippet: Figure 1. Establishment of BETi-resistant cells from NCI-H1975 cells. A, A schematic diagram of the establishment of ABBV-075–resistant variants. B, The IC50 values of ABBV- 075 in the parental cells and the resistant variants were determined by SRB assays. IC50 refers to the concentrations of ABBV-075 that caused 50% inhibition of cell proliferation after 72-hour treatment. C, Cell morphology of parental cells and resistant variants (RMIX, R1, R5, R6, and R9) was imaged under an Olympus CKX53 phase contrast microscope using a 10objectivelens.Bars,100 mm. D, IC50 values of four BETis including ABBV-075, (þ)-JQ1, OTX015, and N2817 in parental cells and resistant variants (RMIX, R1, R5, R6, and R9) were detected by SRB assays after 72-hour treatments. E, Growth rates of indicated cells measured during 1–7-day incubation. Cell viability on each day was detected using CellTiter-Glo. Relative OD values were obtained by comparing the OD values on days 2–7 to that on day 1 and OD values of day 1 were normalized as one. Data from three independent experiments, if applicable, were expressed as mean SD (error bar); , P < 0.05.

    Article Snippet: C, Cell morphology of parental cells and resistant variants (RMIX, R1, R5, R6, and R9) was imaged under an Olympus CKX53 phase contrastmicroscope using a 10 objective lens.

    Techniques: Inhibition, Microscopy, Incubation