Journal: Physiological Reports
Article Title: Subtherapeutic lithium supplementation causes physiological eccentric cardiac hypertrophy in young‐adult wild‐type male mice
doi: 10.14814/phy2.70299
Figure Lengend Snippet: GSK3, SERCA, and PLN content are unchanged with 12 weeks LiCl treatment. Representative Western blots of phosphorylated (Ser9) GSK3β (pGSK3β) and total GSK3β (tGSK3β) (a). Optical density analysis of pGSK3β (b), tGSK3β (c), and their ratio (d). Representative blots of SERCA2 and PLN (e). Optical density analysis of SERCA2 (f), PLN (g), and their ratio (h). For (b–d) and (f–h), a two‐tailed Student's t ‐test was used with exact p values shown for statistically significant ( p < 0.05) or trending ( p < 0.10) comparisons. All values represented as mean ± SD, n = 5–6 per group.
Article Snippet: SERCA2 (MA3‐919; ThermoFisher Scientific; Waltham, MA, USA), PLN (MA3‐922; ThermoFisher Scientific), and total GSK3β (9315; Cell Signaling Technology; Beverly, MA, USA) antibodies were incubated with 5% milk, while pGSK3β (Ser9; 9336; Cell Signaling Technology) antibody was incubated with 3% BSA.
Techniques: Western Blot, Two Tailed Test