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pgl3-181-htert promoter-luciferase reporter plasmid  (Promega)

 
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    Structured Review

    Promega pgl3-181-htert promoter-luciferase reporter plasmid
    Pgl3 181 Htert Promoter Luciferase Reporter Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3-181/pgl3+basic/pm25798839-222-3-40
    Average 90 stars, based on 1 article reviews
    pgl3-181-htert promoter-luciferase reporter plasmid - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Tamoxifen regulates human telomerase reverse transcriptase (hTERT) gene expression differently in breast and endometrial cancer cells.
    Article Snippet: Tamoxifen is widely applied as an antiestrogenic agent for adjuvant therapy in the treatment of breast cancer, while its estrogen-agonistic activity occasionally causes proliferative disorders or carcinogenesis at other sites, such as the uterus.. We reported that estrogen activates telomerase in breast and endometrial cancer cells.. The present study examines the e ects of tamoxifen on the gene expression of human telomerase reverse transcriptase (hTERT) in breast and endometrial cancer cells.

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: Plasmid construction pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Luciferase:

    Article Title: Tamoxifen regulates human telomerase reverse transcriptase (hTERT) gene expression differently in breast and endometrial cancer cells.
    Article Snippet: Tamoxifen is widely applied as an antiestrogenic agent for adjuvant therapy in the treatment of breast cancer, while its estrogen-agonistic activity occasionally causes proliferative disorders or carcinogenesis at other sites, such as the uterus.. We reported that estrogen activates telomerase in breast and endometrial cancer cells.. The present study examines the e ects of tamoxifen on the gene expression of human telomerase reverse transcriptase (hTERT) in breast and endometrial cancer cells.

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: Plasmid construction pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Construct:

    Article Title: Tamoxifen regulates human telomerase reverse transcriptase (hTERT) gene expression differently in breast and endometrial cancer cells.
    Article Snippet: Tamoxifen is widely applied as an antiestrogenic agent for adjuvant therapy in the treatment of breast cancer, while its estrogen-agonistic activity occasionally causes proliferative disorders or carcinogenesis at other sites, such as the uterus.. We reported that estrogen activates telomerase in breast and endometrial cancer cells.. The present study examines the e ects of tamoxifen on the gene expression of human telomerase reverse transcriptase (hTERT) in breast and endometrial cancer cells.

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: Plasmid construction pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Plasmid Preparation:

    Article Title: Tamoxifen regulates human telomerase reverse transcriptase (hTERT) gene expression differently in breast and endometrial cancer cells.
    Article Snippet: Tamoxifen is widely applied as an antiestrogenic agent for adjuvant therapy in the treatment of breast cancer, while its estrogen-agonistic activity occasionally causes proliferative disorders or carcinogenesis at other sites, such as the uterus.. We reported that estrogen activates telomerase in breast and endometrial cancer cells.. The present study examines the e ects of tamoxifen on the gene expression of human telomerase reverse transcriptase (hTERT) in breast and endometrial cancer cells.

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: Plasmid construction pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Expressing:

    Article Title: Tamoxifen regulates human telomerase reverse transcriptase (hTERT) gene expression differently in breast and endometrial cancer cells.
    Article Snippet: Tamoxifen is widely applied as an antiestrogenic agent for adjuvant therapy in the treatment of breast cancer, while its estrogen-agonistic activity occasionally causes proliferative disorders or carcinogenesis at other sites, such as the uterus.. We reported that estrogen activates telomerase in breast and endometrial cancer cells.. The present study examines the e ects of tamoxifen on the gene expression of human telomerase reverse transcriptase (hTERT) in breast and endometrial cancer cells.

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: Plasmid construction pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Transfection:

    Article Title: Tamoxifen regulates human telomerase reverse transcriptase (hTERT) gene expression differently in breast and endometrial cancer cells.
    Article Snippet: Tamoxifen is widely applied as an antiestrogenic agent for adjuvant therapy in the treatment of breast cancer, while its estrogen-agonistic activity occasionally causes proliferative disorders or carcinogenesis at other sites, such as the uterus.. We reported that estrogen activates telomerase in breast and endometrial cancer cells.. The present study examines the e ects of tamoxifen on the gene expression of human telomerase reverse transcriptase (hTERT) in breast and endometrial cancer cells.

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: Plasmid construction pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Incubation:

    Article Title: Tamoxifen regulates human telomerase reverse transcriptase (hTERT) gene expression differently in breast and endometrial cancer cells.
    Article Snippet: Tamoxifen is widely applied as an antiestrogenic agent for adjuvant therapy in the treatment of breast cancer, while its estrogen-agonistic activity occasionally causes proliferative disorders or carcinogenesis at other sites, such as the uterus.. We reported that estrogen activates telomerase in breast and endometrial cancer cells.. The present study examines the e ects of tamoxifen on the gene expression of human telomerase reverse transcriptase (hTERT) in breast and endometrial cancer cells.

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: Plasmid construction pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Activity Assay:

    Article Title: Tamoxifen regulates human telomerase reverse transcriptase (hTERT) gene expression differently in breast and endometrial cancer cells.
    Article Snippet: Tamoxifen is widely applied as an antiestrogenic agent for adjuvant therapy in the treatment of breast cancer, while its estrogen-agonistic activity occasionally causes proliferative disorders or carcinogenesis at other sites, such as the uterus.. We reported that estrogen activates telomerase in breast and endometrial cancer cells.. The present study examines the e ects of tamoxifen on the gene expression of human telomerase reverse transcriptase (hTERT) in breast and endometrial cancer cells.

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: Plasmid construction pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Dominant Negative Mutation:

    Article Title: Tamoxifen regulates human telomerase reverse transcriptase (hTERT) gene expression differently in breast and endometrial cancer cells.
    Article Snippet: Tamoxifen is widely applied as an antiestrogenic agent for adjuvant therapy in the treatment of breast cancer, while its estrogen-agonistic activity occasionally causes proliferative disorders or carcinogenesis at other sites, such as the uterus.. We reported that estrogen activates telomerase in breast and endometrial cancer cells.. The present study examines the e ects of tamoxifen on the gene expression of human telomerase reverse transcriptase (hTERT) in breast and endometrial cancer cells.

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: Plasmid construction pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).

    Article Title: Telomerase activation by histone deacetylase inhibitor in normal cells
    Article Snippet: pGL3-3328 and pGL3-181 are hTERT promoter-luciferase reporter plasmids in which 3328 and 181 bp sequences upstream of the transcription start site (numbered as +1) as well as a 77 bp 5′-untranslated region are cloned upstream of the luciferase gene (LUC) in pGL3-Basic (Promega) at Mlu I and Bgl II sites ( 8 ).



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