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vector pgbkt7 bd  (TaKaRa)


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    TaKaRa vector pgbkt7 bd
    Vector Pgbkt7 Bd, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4537 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pgbkt7 bd/product/TaKaRa
    Average 99 stars, based on 4537 article reviews
    vector pgbkt7 bd - by Bioz Stars, 2026-05
    99/100 stars

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    Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a <t>GAL4‐activating</t> domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.
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    Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.

    Journal: Molecular Plant Pathology

    Article Title: Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and Cucumbers

    doi: 10.1111/mpp.70202

    Figure Lengend Snippet: Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with Y187 yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.

    Article Snippet: Full‐length ORFs of REn were obtained from ToLCNDV‐C, ToLCNDV‐C[M9], ToLCNDV‐T&C and ToLCKV‐T plasmids and cloned into pGBKT7‐BD, fusing with the Gal4 DNA‐binding domain (Takara: 630443).

    Techniques: Expressing, Binding Assay, Selection, Plasmid Preparation