pgadt7 vector (TaKaRa)
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TaKaRa
pgadt7 vector
Pgadt7 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15972 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgadt7/EcoR+I/pmc13195091-394-17-19
Average 99 stars, based on 15972 article reviews
Pgadt7 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15972 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgadt7/EcoR+I/pmc13195091-394-17-19
Average 99 stars, based on 15972 article reviews
pgadt7 vector - by Bioz Stars,
2026-09
99/100 stars
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Polymerase Chain Reaction:Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: .. For yeast two-hybrid assays with M. musculus proteins, M. musculus SYCP2 fragments were PCR-amplified and cloned into the NdeI site of Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: After a 10 min incubation at room temperature, fluorescence polarization was read using a Tecan SPARK fluorescence plate reader, and binding data were analyzed with Graphpad Prism version 10 using a single-site binding model. .. For yeast two-hybrid assays with A. thaliana proteins, A. thaliana ASY3 fragments were PCR-amplified and cloned into the NdeI site of Article Title: Integrative regulation of axillary meristem maturation and stolon fate determination in strawberry by light, gibberellin, and ZFP6. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Clone Assay:Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: .. For yeast two-hybrid assays with M. musculus proteins, M. musculus SYCP2 fragments were PCR-amplified and cloned into the NdeI site of Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: After a 10 min incubation at room temperature, fluorescence polarization was read using a Tecan SPARK fluorescence plate reader, and binding data were analyzed with Graphpad Prism version 10 using a single-site binding model. .. For yeast two-hybrid assays with A. thaliana proteins, A. thaliana ASY3 fragments were PCR-amplified and cloned into the NdeI site of Article Title: Overexpression of MtDof32 in Medicago truncatula enhances leaf and flower organ size through modulation of cell expansion Article Snippet: Yeast two-hybrid screening was conducted using the Matchmaker GAL4 Two-Hybrid System (Clontech, USA), where the Y187 strain carrying the bait construct was mated with AH109 strain containing a M. truncatula cDNA library for protein interaction screening. .. For prey vector construction, the MtEBP1 CDS was cloned into Article Title: QUIRKY controls seed germination via precision degradation of ABI5 Article Snippet: To quantify His-mCherry-ABI5 condensates in the presence of His-GFP-QKY N834 in vitro, condensate number and intensity were similarly assessed using the Analyze Particles function in ImageJ. .. The coding regions of ABI5 , KEG , the N-terminal fragments of QKY , devoid of the sequences encoding the transmembrane regions, and various truncated versions of QKY and KEG were amplified and cloned into pGBKT7 and Article Title: Overexpression of MtDof32 in Medicago truncatula enhances leaf and flower organ size through modulation of cell expansion Article Snippet: Yeast two-hybrid screening was conducted using the Matchmaker GAL4 Two-Hybrid System (Clontech, USA), where the Y187 strain carrying the bait construct was mated with AH109 strain containing a M. truncatula cDNA library for protein interaction screening. .. For prey vector construction, the MtEBP1 CDS was cloned into Article Title: A Conserved Magnaporthe oryzae Effector Counteracts the Rice Ubiquitin-Proteasome System by Disrupting the E2 Function to Suppress Immunity. Article Snippet: .. Briefly, the fragment of OsRING10C, OsUBC11 and MoCE1ΔSP were amplified and cloned into the Activation Assay:Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: .. For yeast two-hybrid assays with M. musculus proteins, M. musculus SYCP2 fragments were PCR-amplified and cloned into the NdeI site of Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: After a 10 min incubation at room temperature, fluorescence polarization was read using a Tecan SPARK fluorescence plate reader, and binding data were analyzed with Graphpad Prism version 10 using a single-site binding model. .. For yeast two-hybrid assays with A. thaliana proteins, A. thaliana ASY3 fragments were PCR-amplified and cloned into the NdeI site of Article Title: Recreating viable YY h genotype uncovers the role of CpYYL underlying YY lethality in papaya. Article Snippet: For pGBKT7 (Clontech) bait vectors, the truncated coding sequences of CpYYL were fused downstream of the GAL4 DNA binding domain to generate BD recombinant plasmid. .. For Plasmid Preparation:Article Title: Overexpression of MtDof32 in Medicago truncatula enhances leaf and flower organ size through modulation of cell expansion Article Snippet: Yeast two-hybrid screening was conducted using the Matchmaker GAL4 Two-Hybrid System (Clontech, USA), where the Y187 strain carrying the bait construct was mated with AH109 strain containing a M. truncatula cDNA library for protein interaction screening. .. For prey vector construction, the MtEBP1 CDS was cloned into Article Title: Overexpression of MtDof32 in Medicago truncatula enhances leaf and flower organ size through modulation of cell expansion Article Snippet: Yeast two-hybrid screening was conducted using the Matchmaker GAL4 Two-Hybrid System (Clontech, USA), where the Y187 strain carrying the bait construct was mated with AH109 strain containing a M. truncatula cDNA library for protein interaction screening. .. For prey vector construction, the MtEBP1 CDS was cloned into Article Title: Recreating viable YY h genotype uncovers the role of CpYYL underlying YY lethality in papaya. Article Snippet: For pGBKT7 (Clontech) bait vectors, the truncated coding sequences of CpYYL were fused downstream of the GAL4 DNA binding domain to generate BD recombinant plasmid. .. For Transformation Assay:Article Title: Overexpression of MtDof32 in Medicago truncatula enhances leaf and flower organ size through modulation of cell expansion Article Snippet: Yeast two-hybrid screening was conducted using the Matchmaker GAL4 Two-Hybrid System (Clontech, USA), where the Y187 strain carrying the bait construct was mated with AH109 strain containing a M. truncatula cDNA library for protein interaction screening. .. For prey vector construction, the MtEBP1 CDS was cloned into Article Title: Overexpression of MtDof32 in Medicago truncatula enhances leaf and flower organ size through modulation of cell expansion Article Snippet: Yeast two-hybrid screening was conducted using the Matchmaker GAL4 Two-Hybrid System (Clontech, USA), where the Y187 strain carrying the bait construct was mated with AH109 strain containing a M. truncatula cDNA library for protein interaction screening. .. For prey vector construction, the MtEBP1 CDS was cloned into Sequencing:Article Title: Integrative regulation of axillary meristem maturation and stolon fate determination in strawberry by light, gibberellin, and ZFP6. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Amplification:Article Title: Integrative regulation of axillary meristem maturation and stolon fate determination in strawberry by light, gibberellin, and ZFP6. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. Article Title: QUIRKY controls seed germination via precision degradation of ABI5 Article Snippet: To quantify His-mCherry-ABI5 condensates in the presence of His-GFP-QKY N834 in vitro, condensate number and intensity were similarly assessed using the Analyze Particles function in ImageJ. .. The coding regions of ABI5 , KEG , the N-terminal fragments of QKY , devoid of the sequences encoding the transmembrane regions, and various truncated versions of QKY and KEG were amplified and cloned into pGBKT7 and Article Title: A Conserved Magnaporthe oryzae Effector Counteracts the Rice Ubiquitin-Proteasome System by Disrupting the E2 Function to Suppress Immunity. Article Snippet: .. Briefly, the fragment of OsRING10C, OsUBC11 and MoCE1ΔSP were amplified and cloned into the Recombinant:Article Title: Recreating viable YY h genotype uncovers the role of CpYYL underlying YY lethality in papaya. Article Snippet: For pGBKT7 (Clontech) bait vectors, the truncated coding sequences of CpYYL were fused downstream of the GAL4 DNA binding domain to generate BD recombinant plasmid. .. For |