h19-pflag-cmv-2 expression vector (Millipore)
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Millipore
h19-pflag-cmv-2 expression vector
H19 Pflag Cmv 2 Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pflag-cmv-2/pflag+cmv2/pmc11299361-54-10-16
Average 90 stars, based on 1 article reviews
H19 Pflag Cmv 2 Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pflag-cmv-2/pflag+cmv2/pmc11299361-54-10-16
Average 90 stars, based on 1 article reviews
h19-pflag-cmv-2 expression vector - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Regulatory role of lncH19 in RAC1 alternative splicing: implication for RAC1B expression in colorectal cancer"
Article Title: Regulatory role of lncH19 in RAC1 alternative splicing: implication for RAC1B expression in colorectal cancer
Journal: Journal of Experimental & Clinical Cancer Research : CR
doi: 10.1186/s13046-024-03139-z
Figure Legend Snippet: The sequence of the primers used for gene expression analysis
Techniques Used: Sequencing, Expressing
Figure Legend Snippet: The sequence of the biotin-labeled H19 probes used for RNA pull-down assays
Techniques Used: Sequencing
Figure Legend Snippet: RBFOX2 is involved in RAC1 alternative splicing in CRC cells. ( A - B - D , E - F - H ) QRT-PCR for the indicated mRNA in CRC cells, silenced for RBFOX2 with two different siRNA. Graphs show 2-ΔΔct calculated in silenced cells respect to relative controls (Normality test and t-test). ( C , F ) Western Blot and densitometric analyses for RAC1B in SW620 and HCT116 silenced for RBFOX2 and relative controls. For densitometric analysis data are represented as normalized OD. ( I - L ) qRT-PCR of the indicated genes in SW620 and HCT116 silenced for H19, the graphs represent the 2^- ΔΔ ct of the indicated calculated respect the expression in control cells. ( M ) Western Blot of RAC1b protein levels in CRC cells (SW620 and HCT116) in H19 silenced cells and relative control cells. Statistical analyses were performed using normality test and t-test, p-value is shown in th e graphs
Techniques Used: Alternative Splicing, Quantitative RT-PCR, Western Blot, Expressing, Control
Figure Legend Snippet: Overexpression of lncH19 upregulates RAC1B expression. ( A ) qRT-PCR to analyze H19 mRNA levels in CRC cells (SW620 and HCT116) transfected with lncH19 or empty vector. ( B ) qRT-PCR to analyze RAC1B mRNA levels in CRC cells (SW620 and HCT116) transfected with lncH19 or empty vector. ( C ) Western Blot for RAC1B in CRC cells (SW620 and HCT116), transfected with lncH19 or empty vector. lncH19 overexpression promotes RAC1B nuclear localization. ( D ) Representative confocal microscopy images of anti-RAC1b immunofluorescence showed nuclear localization of RAC1b in lncH19-overexpressing CRC cells, SW620 (upper panel) and HCT116 (lower panel). The analysis of the RAC1B nuclear signal is reported on the histogram. ( E ) Western blot analysis and densitometric analyses for RAC1B in nuclear protein fractions of CRC cells (SW620 and HCT116) overexpressing H19 cells and relative control cells. E-F) Overexpression of lncH19 upregulate CiclinD and c-Myc expression through RAC1B activity qRT-PCR for the indicated genes in SW620 ( F ) and HCT116 ( G ) transfected with pH19 or empty vector and treated or not with RAC1B inhibitor. Statistical analyses were performed using: normality test, one sample t-test to compare different conditions to pEmpty untreated cells, and Two-tails unpaired t-test to compare two groups *; p-value is shown in the graphs
Techniques Used: Over Expression, Expressing, Quantitative RT-PCR, Transfection, Plasmid Preparation, Western Blot, Confocal Microscopy, Immunofluorescence, Control, Activity Assay
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Article Snippet: To construct an expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the FLAG epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR amplified and inserted into the EcoRI and BamHI sites of Article Title: Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein Article Snippet: CDK5RAP2 fragments were subcloned into Article Title: Deficiency in 3′-phosphoglycolate processing in human cells with a hereditary mutation in tyrosyl-DNA phosphodiesterase (TDP1) Article Snippet: In order to overexpress TDP1 in human cells, the 1.8 kb BamHI/Bsu36I fragment (with the Bsu36I site converted to a blunt end by fill-in with T4 DNA polymerase) was isolated from pHN1894S ( ) (generous gift from Howard Nash and Jeff Pouliot, NIMH) and cloned into the BglII and EcoRV sites of the Article Title: Interaction and assembly of the DNA replication core proteins of Kaposi's sarcoma-associated herpesvirus. Article Snippet: To generate the expression plasmids encoding ORF6, ORF9, ORF40/41, ORF44, ORF56, and ORF59, the corresponding DNA fragments amplified by PCR were cloned into Article Title: Inhibition of CIN85-Mediated Invasion by a Novel SH3 Domain Binding Motif in the Lysyl Oxidase Propeptide Article Snippet: The cDNA encoding rCIN85 WT was digested with Eco RI and subcloned into pGEX4T-3 (GE Healthcare, Uppsala, Sweden) and Article Title: Herpes simplex type 1 UL43 multiple membrane-spanning protein increases energy metabolism in of host cells through interacting with ARL2 Article Snippet: To construct expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the Flag epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR ampli ed and inserted into the EcoRI and BamHI sites of Article Title: Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria Article Snippet: The wild-type coding sequence of ADAR1 was amplified from pCMV6-ADAR1 (Origene) by PCR and subcloned into Isolation:Article Title: HAP1 can sequester a subset of TBP in cytoplasmic inclusions via specific interaction with the conserved TBP CORE Article Snippet: The TBP-FL cDNA was inserted between the Sal I and Not I sites in both pCMV-HA (BD Biosciences) and pFLAG-CMV-2 SN , which is a modified version of Article Title: Herpes Simplex Type 1 UL43 Multiple Membrane-Spanning Protein Increases Energy Metabolism in Host Cells through Interacting with ARL2. Article Snippet: To construct an expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the FLAG epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR amplified and inserted into the EcoRI and BamHI sites of Article Title: Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein Article Snippet: CDK5RAP2 fragments were subcloned into Article Title: Deficiency in 3′-phosphoglycolate processing in human cells with a hereditary mutation in tyrosyl-DNA phosphodiesterase (TDP1) Article Snippet: In order to overexpress TDP1 in human cells, the 1.8 kb BamHI/Bsu36I fragment (with the Bsu36I site converted to a blunt end by fill-in with T4 DNA polymerase) was isolated from pHN1894S ( ) (generous gift from Howard Nash and Jeff Pouliot, NIMH) and cloned into the BglII and EcoRV sites of the Article Title: Interaction and assembly of the DNA replication core proteins of Kaposi's sarcoma-associated herpesvirus. Article Snippet: To generate the expression plasmids encoding ORF6, ORF9, ORF40/41, ORF44, ORF56, and ORF59, the corresponding DNA fragments amplified by PCR were cloned into Article Title: Inhibition of CIN85-Mediated Invasion by a Novel SH3 Domain Binding Motif in the Lysyl Oxidase Propeptide Article Snippet: The cDNA encoding rCIN85 WT was digested with Eco RI and subcloned into pGEX4T-3 (GE Healthcare, Uppsala, Sweden) and Article Title: Herpes simplex type 1 UL43 multiple membrane-spanning protein increases energy metabolism in of host cells through interacting with ARL2 Article Snippet: To construct expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the Flag epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR ampli ed and inserted into the EcoRI and BamHI sites of Article Title: Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria Article Snippet: The wild-type coding sequence of ADAR1 was amplified from pCMV6-ADAR1 (Origene) by PCR and subcloned into Plasmid Preparation:Article Title: HAP1 can sequester a subset of TBP in cytoplasmic inclusions via specific interaction with the conserved TBP CORE Article Snippet: The TBP-FL cDNA was inserted between the Sal I and Not I sites in both pCMV-HA (BD Biosciences) and pFLAG-CMV-2 SN , which is a modified version of Article Title: Herpes Simplex Type 1 UL43 Multiple Membrane-Spanning Protein Increases Energy Metabolism in Host Cells through Interacting with ARL2. Article Snippet: To construct an expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the FLAG epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR amplified and inserted into the EcoRI and BamHI sites of Article Title: Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein Article Snippet: CDK5RAP2 fragments were subcloned into Article Title: Deficiency in 3′-phosphoglycolate processing in human cells with a hereditary mutation in tyrosyl-DNA phosphodiesterase (TDP1) Article Snippet: In order to overexpress TDP1 in human cells, the 1.8 kb BamHI/Bsu36I fragment (with the Bsu36I site converted to a blunt end by fill-in with T4 DNA polymerase) was isolated from pHN1894S ( ) (generous gift from Howard Nash and Jeff Pouliot, NIMH) and cloned into the BglII and EcoRV sites of the Article Title: Interaction and assembly of the DNA replication core proteins of Kaposi's sarcoma-associated herpesvirus. Article Snippet: To generate the expression plasmids encoding ORF6, ORF9, ORF40/41, ORF44, ORF56, and ORF59, the corresponding DNA fragments amplified by PCR were cloned into Article Title: Inhibition of CIN85-Mediated Invasion by a Novel SH3 Domain Binding Motif in the Lysyl Oxidase Propeptide Article Snippet: The cDNA encoding rCIN85 WT was digested with Eco RI and subcloned into pGEX4T-3 (GE Healthcare, Uppsala, Sweden) and Article Title: Herpes simplex type 1 UL43 multiple membrane-spanning protein increases energy metabolism in of host cells through interacting with ARL2 Article Snippet: To construct expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the Flag epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR ampli ed and inserted into the EcoRI and BamHI sites of Article Title: Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria Article Snippet: The wild-type coding sequence of ADAR1 was amplified from pCMV6-ADAR1 (Origene) by PCR and subcloned into Modification:Article Title: HAP1 can sequester a subset of TBP in cytoplasmic inclusions via specific interaction with the conserved TBP CORE Article Snippet: The TBP-FL cDNA was inserted between the Sal I and Not I sites in both pCMV-HA (BD Biosciences) and pFLAG-CMV-2 SN , which is a modified version of Article Title: Herpes Simplex Type 1 UL43 Multiple Membrane-Spanning Protein Increases Energy Metabolism in Host Cells through Interacting with ARL2. Article Snippet: To construct an expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the FLAG epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR amplified and inserted into the EcoRI and BamHI sites of Article Title: Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein Article Snippet: CDK5RAP2 fragments were subcloned into Article Title: Deficiency in 3′-phosphoglycolate processing in human cells with a hereditary mutation in tyrosyl-DNA phosphodiesterase (TDP1) Article Snippet: In order to overexpress TDP1 in human cells, the 1.8 kb BamHI/Bsu36I fragment (with the Bsu36I site converted to a blunt end by fill-in with T4 DNA polymerase) was isolated from pHN1894S ( ) (generous gift from Howard Nash and Jeff Pouliot, NIMH) and cloned into the BglII and EcoRV sites of the Article Title: Interaction and assembly of the DNA replication core proteins of Kaposi's sarcoma-associated herpesvirus. Article Snippet: To generate the expression plasmids encoding ORF6, ORF9, ORF40/41, ORF44, ORF56, and ORF59, the corresponding DNA fragments amplified by PCR were cloned into Article Title: Inhibition of CIN85-Mediated Invasion by a Novel SH3 Domain Binding Motif in the Lysyl Oxidase Propeptide Article Snippet: The cDNA encoding rCIN85 WT was digested with Eco RI and subcloned into pGEX4T-3 (GE Healthcare, Uppsala, Sweden) and Article Title: Herpes simplex type 1 UL43 multiple membrane-spanning protein increases energy metabolism in of host cells through interacting with ARL2 Article Snippet: To construct expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the Flag epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR ampli ed and inserted into the EcoRI and BamHI sites of Article Title: Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria Article Snippet: The wild-type coding sequence of ADAR1 was amplified from pCMV6-ADAR1 (Origene) by PCR and subcloned into Construct:Article Title: HAP1 can sequester a subset of TBP in cytoplasmic inclusions via specific interaction with the conserved TBP CORE Article Snippet: The TBP-FL cDNA was inserted between the Sal I and Not I sites in both pCMV-HA (BD Biosciences) and pFLAG-CMV-2 SN , which is a modified version of Article Title: Herpes Simplex Type 1 UL43 Multiple Membrane-Spanning Protein Increases Energy Metabolism in Host Cells through Interacting with ARL2. Article Snippet: To construct an expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the FLAG epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR amplified and inserted into the EcoRI and BamHI sites of Article Title: Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein Article Snippet: CDK5RAP2 fragments were subcloned into Article Title: Deficiency in 3′-phosphoglycolate processing in human cells with a hereditary mutation in tyrosyl-DNA phosphodiesterase (TDP1) Article Snippet: In order to overexpress TDP1 in human cells, the 1.8 kb BamHI/Bsu36I fragment (with the Bsu36I site converted to a blunt end by fill-in with T4 DNA polymerase) was isolated from pHN1894S ( ) (generous gift from Howard Nash and Jeff Pouliot, NIMH) and cloned into the BglII and EcoRV sites of the Article Title: Interaction and assembly of the DNA replication core proteins of Kaposi's sarcoma-associated herpesvirus. Article Snippet: To generate the expression plasmids encoding ORF6, ORF9, ORF40/41, ORF44, ORF56, and ORF59, the corresponding DNA fragments amplified by PCR were cloned into Article Title: Inhibition of CIN85-Mediated Invasion by a Novel SH3 Domain Binding Motif in the Lysyl Oxidase Propeptide Article Snippet: The cDNA encoding rCIN85 WT was digested with Eco RI and subcloned into pGEX4T-3 (GE Healthcare, Uppsala, Sweden) and Article Title: Herpes simplex type 1 UL43 multiple membrane-spanning protein increases energy metabolism in of host cells through interacting with ARL2 Article Snippet: To construct expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the Flag epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR ampli ed and inserted into the EcoRI and BamHI sites of Article Title: Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria Article Snippet: The wild-type coding sequence of ADAR1 was amplified from pCMV6-ADAR1 (Origene) by PCR and subcloned into FLAG-tag:Article Title: HAP1 can sequester a subset of TBP in cytoplasmic inclusions via specific interaction with the conserved TBP CORE Article Snippet: The TBP-FL cDNA was inserted between the Sal I and Not I sites in both pCMV-HA (BD Biosciences) and pFLAG-CMV-2 SN , which is a modified version of Article Title: Herpes Simplex Type 1 UL43 Multiple Membrane-Spanning Protein Increases Energy Metabolism in Host Cells through Interacting with ARL2. Article Snippet: To construct an expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the FLAG epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR amplified and inserted into the EcoRI and BamHI sites of Article Title: Dynamic Recruitment of CDK5RAP2 to Centrosomes Requires Its Association with Dynein Article Snippet: CDK5RAP2 fragments were subcloned into Article Title: Deficiency in 3′-phosphoglycolate processing in human cells with a hereditary mutation in tyrosyl-DNA phosphodiesterase (TDP1) Article Snippet: In order to overexpress TDP1 in human cells, the 1.8 kb BamHI/Bsu36I fragment (with the Bsu36I site converted to a blunt end by fill-in with T4 DNA polymerase) was isolated from pHN1894S ( ) (generous gift from Howard Nash and Jeff Pouliot, NIMH) and cloned into the BglII and EcoRV sites of the Article Title: Interaction and assembly of the DNA replication core proteins of Kaposi's sarcoma-associated herpesvirus. Article Snippet: To generate the expression plasmids encoding ORF6, ORF9, ORF40/41, ORF44, ORF56, and ORF59, the corresponding DNA fragments amplified by PCR were cloned into Article Title: Inhibition of CIN85-Mediated Invasion by a Novel SH3 Domain Binding Motif in the Lysyl Oxidase Propeptide Article Snippet: The cDNA encoding rCIN85 WT was digested with Eco RI and subcloned into pGEX4T-3 (GE Healthcare, Uppsala, Sweden) and Article Title: Herpes simplex type 1 UL43 multiple membrane-spanning protein increases energy metabolism in of host cells through interacting with ARL2 Article Snippet: To construct expression vector pCMV(f)UL43-WT, whose UL43 gene was tagged with only the Flag epitope sequence (DYKDDDDK), a UL43 ORF without a start codon was PCR ampli ed and inserted into the EcoRI and BamHI sites of Article Title: Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria Article Snippet: The wild-type coding sequence of ADAR1 was amplified from pCMV6-ADAR1 (Origene) by PCR and subcloned into |