Journal: iScience
Article Title: APOE4 drives widespread changes to the hepatic proteome and alters metabolic function
doi: 10.1016/j.isci.2026.115035
Figure Lengend Snippet: Isolated liver mitochondrial proteomics in male and female APOE3 or APOE4 mice (A) Western blot analysis assessing purity of liver mitochondrial isolates. Blots were probed for HDAC1, HK1, and CS. (B) Pie chart showing the proportion of proteins identified in isolated liver mitochondrial samples that are annotated as mitochondrial in the MitoCarta3.0 database. (C) Volcano plot showing upregulated and downregulated proteins between female APOE4 and APOE3 mice. (D) Volcano plot showing upregulated and downregulated proteins between male APOE4 and APOE3 mice. (E) IPA pathway analysis showing top upregulated and downregulated pathways between female APOE4 and APOE3 mice. (F) IPA pathway analysis showing top upregulated and downregulated pathways between male APOE4 and APOE3 mice. (G) Heatmap of oxidative phosphorylation complex I–V components between APOE4 and APOE3 female and male mice. n = 3 for proteomic outcomes. (H) Heatmap comparing whole liver to isolated mitochondria results of proteins involved in mitochondrial protein import and degradation between APOE4 and APOE3 female mice. (I) Heatmap of proteins involved in TCA cycle and pyruvate metabolism between APOE4 and APOE3 female and male mice. See also .
Article Snippet: Primary antibodies included ALB (Abcam, ab207327), APOE (Abcam, ab183597), ASGR1 (Abcam, ab254261), CS (Cell Signaling, #14309), CYP27A1 (Abcam, ab126785), FDFT1 (Proteintech, 83020-1-RR), HDAC1 (Cell Signaling, #34589), HK1 (Cell Signaling, #2024), HNF4A (Abcam, ab92378), NANOG (Abcam, ab109250), OCT4 (Abcam, ab181557), PGAM1 (Cell Signaling, #12098), and SOX17 (Abcam, ab224637).
Techniques: Isolation, Western Blot, Phospho-proteomics
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