Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
Article Title: Aging impairs peroxisome biogenesis in human B cells
doi: 10.1093/gerona/glaf148
Figure Lengend Snippet: Peroxisome number decreases in human immune cell aging. ( A) Immunofluorescence staining of PEX14 in CD4 + T cells isolated from young (23-year old) and old individuals (74-year old). Hoechst 33342 was used for nuclear staining. Scale bar: 2 μm. (B) Immunofluorescence staining of PEX14 in CD8 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (C) Immunofluorescence staining of PEX14 in CD14 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (D) Immunofluorescence staining of PEX14 in CD19 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (E) Quantification of PEX14-positive puncta in young individuals. one-way ANOVA, ns: not significant, n = 7. (F) Quantification of PEX14-positive puncta in four immune cell types isolated from young and old individuals. Student’s t -test, n = 7. Data are represented as mean ± SD. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).
Article Snippet: The primary and secondary antibodies are summarized below: anti-CD19 (Proteintech #66298-1-Ig, 1:200), anti-CD14 (Proteintech #60253-1-Ig, 1:200), anti-CD4 (Proteintech #67786-1-Ig, 1:200), anti-CD8 (Proteintech #66868-1-Ig, 1:200), anti-SKL (Gift from Richard Rachubinski lab, 1:200), CoraLite ® 594-conjugated PEX14 Polyclonal antibody (Proteintech # CL594-10594, 1:200), Alexa Flour ® 488 anti-mouse IgG (Jackson ImmunoResearch #715-545-150, 1:500), Alexa Fluor ® 594 anti-Rabbit IgG (Jackson ImmunoResearch #711-585-152, 1:500).
Techniques: Immunofluorescence, Staining, Isolation