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antibody pex14 alexa fluor 488 conjugated  (Proteintech)


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    Structured Review

    Proteintech antibody pex14 alexa fluor 488 conjugated
    Antibody Pex14 Alexa Fluor 488 Conjugated, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody pex14 alexa fluor 488 conjugated/product/Proteintech
    Average 94 stars, based on 78 article reviews
    antibody pex14 alexa fluor 488 conjugated - by Bioz Stars, 2026-04
    94/100 stars

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    Peroxisome number decreases in human immune cell aging. ( A) Immunofluorescence staining of <t>PEX14</t> in CD4 + T cells isolated from young (23-year old) and old individuals (74-year old). Hoechst 33342 was used for nuclear staining. Scale bar: 2 μm. (B) Immunofluorescence staining of PEX14 in CD8 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (C) Immunofluorescence staining of PEX14 in CD14 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (D) Immunofluorescence staining of PEX14 in CD19 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (E) Quantification of PEX14-positive puncta in young individuals. one-way ANOVA, ns: not significant, n = 7. (F) Quantification of PEX14-positive puncta in four immune cell types isolated from young and old individuals. Student’s t -test, n = 7. Data are represented as mean ± SD. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).
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    Peroxisome number decreases in human immune cell aging. ( A) Immunofluorescence staining of <t>PEX14</t> in CD4 + T cells isolated from young (23-year old) and old individuals (74-year old). Hoechst 33342 was used for nuclear staining. Scale bar: 2 μm. (B) Immunofluorescence staining of PEX14 in CD8 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (C) Immunofluorescence staining of PEX14 in CD14 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (D) Immunofluorescence staining of PEX14 in CD19 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (E) Quantification of PEX14-positive puncta in young individuals. one-way ANOVA, ns: not significant, n = 7. (F) Quantification of PEX14-positive puncta in four immune cell types isolated from young and old individuals. Student’s t -test, n = 7. Data are represented as mean ± SD. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).
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    Peroxisome number decreases in human immune cell aging. ( A) Immunofluorescence staining of <t>PEX14</t> in CD4 + T cells isolated from young (23-year old) and old individuals (74-year old). Hoechst 33342 was used for nuclear staining. Scale bar: 2 μm. (B) Immunofluorescence staining of PEX14 in CD8 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (C) Immunofluorescence staining of PEX14 in CD14 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (D) Immunofluorescence staining of PEX14 in CD19 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (E) Quantification of PEX14-positive puncta in young individuals. one-way ANOVA, ns: not significant, n = 7. (F) Quantification of PEX14-positive puncta in four immune cell types isolated from young and old individuals. Student’s t -test, n = 7. Data are represented as mean ± SD. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).
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    Average 94 stars, based on 1 article reviews
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    Proteintech pex14
    Peroxisome number decreases in human immune cell aging. ( A) Immunofluorescence staining of <t>PEX14</t> in CD4 + T cells isolated from young (23-year old) and old individuals (74-year old). Hoechst 33342 was used for nuclear staining. Scale bar: 2 μm. (B) Immunofluorescence staining of PEX14 in CD8 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (C) Immunofluorescence staining of PEX14 in CD14 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (D) Immunofluorescence staining of PEX14 in CD19 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (E) Quantification of PEX14-positive puncta in young individuals. one-way ANOVA, ns: not significant, n = 7. (F) Quantification of PEX14-positive puncta in four immune cell types isolated from young and old individuals. Student’s t -test, n = 7. Data are represented as mean ± SD. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).
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    Image Search Results


    Peroxisome number decreases in human immune cell aging. ( A) Immunofluorescence staining of PEX14 in CD4 + T cells isolated from young (23-year old) and old individuals (74-year old). Hoechst 33342 was used for nuclear staining. Scale bar: 2 μm. (B) Immunofluorescence staining of PEX14 in CD8 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (C) Immunofluorescence staining of PEX14 in CD14 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (D) Immunofluorescence staining of PEX14 in CD19 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (E) Quantification of PEX14-positive puncta in young individuals. one-way ANOVA, ns: not significant, n = 7. (F) Quantification of PEX14-positive puncta in four immune cell types isolated from young and old individuals. Student’s t -test, n = 7. Data are represented as mean ± SD. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).

    Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

    Article Title: Aging impairs peroxisome biogenesis in human B cells

    doi: 10.1093/gerona/glaf148

    Figure Lengend Snippet: Peroxisome number decreases in human immune cell aging. ( A) Immunofluorescence staining of PEX14 in CD4 + T cells isolated from young (23-year old) and old individuals (74-year old). Hoechst 33342 was used for nuclear staining. Scale bar: 2 μm. (B) Immunofluorescence staining of PEX14 in CD8 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (C) Immunofluorescence staining of PEX14 in CD14 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (D) Immunofluorescence staining of PEX14 in CD19 + T cells isolated from young (23-year old) and old individuals (74-year old). Scale bar: 2 μm. (E) Quantification of PEX14-positive puncta in young individuals. one-way ANOVA, ns: not significant, n = 7. (F) Quantification of PEX14-positive puncta in four immune cell types isolated from young and old individuals. Student’s t -test, n = 7. Data are represented as mean ± SD. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).

    Article Snippet: The primary and secondary antibodies are summarized below: anti-CD19 (Proteintech #66298-1-Ig, 1:200), anti-CD14 (Proteintech #60253-1-Ig, 1:200), anti-CD4 (Proteintech #67786-1-Ig, 1:200), anti-CD8 (Proteintech #66868-1-Ig, 1:200), anti-SKL (Gift from Richard Rachubinski lab, 1:200), CoraLite ® 594-conjugated PEX14 Polyclonal antibody (Proteintech # CL594-10594, 1:200), Alexa Flour ® 488 anti-mouse IgG (Jackson ImmunoResearch #715-545-150, 1:500), Alexa Fluor ® 594 anti-Rabbit IgG (Jackson ImmunoResearch #711-585-152, 1:500).

    Techniques: Immunofluorescence, Staining, Isolation

    Peroxisome biogenesis factor PEX19 is downregulated in aged B cells. ( A) Western blot analysis of PEX14 in young and old CD19 + B cells. (B) The quantification of band intensity of PEX14 normalized to beta-actin. Student’s t -test, n = 7. Data are represented as mean ± SD. (C) Linear regression plot showing the correlation relationship between PEX14 expression and age. (D) Western blot analysis of PEX19 in young and old CD19 + B cells. (E) The quantification of band intensity of PEX19 normalized to beta-actin. Student’s t -test, n = 7. Data are represented as mean ± SD. (F) Linear regression plot showing the correlation relationship between PEX19 expression and age. (G) Linear regression plot showing the correlation relationship between PEX14 and PEX19 protein expression. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).

    Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

    Article Title: Aging impairs peroxisome biogenesis in human B cells

    doi: 10.1093/gerona/glaf148

    Figure Lengend Snippet: Peroxisome biogenesis factor PEX19 is downregulated in aged B cells. ( A) Western blot analysis of PEX14 in young and old CD19 + B cells. (B) The quantification of band intensity of PEX14 normalized to beta-actin. Student’s t -test, n = 7. Data are represented as mean ± SD. (C) Linear regression plot showing the correlation relationship between PEX14 expression and age. (D) Western blot analysis of PEX19 in young and old CD19 + B cells. (E) The quantification of band intensity of PEX19 normalized to beta-actin. Student’s t -test, n = 7. Data are represented as mean ± SD. (F) Linear regression plot showing the correlation relationship between PEX19 expression and age. (G) Linear regression plot showing the correlation relationship between PEX14 and PEX19 protein expression. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).

    Article Snippet: The primary and secondary antibodies are summarized below: anti-CD19 (Proteintech #66298-1-Ig, 1:200), anti-CD14 (Proteintech #60253-1-Ig, 1:200), anti-CD4 (Proteintech #67786-1-Ig, 1:200), anti-CD8 (Proteintech #66868-1-Ig, 1:200), anti-SKL (Gift from Richard Rachubinski lab, 1:200), CoraLite ® 594-conjugated PEX14 Polyclonal antibody (Proteintech # CL594-10594, 1:200), Alexa Flour ® 488 anti-mouse IgG (Jackson ImmunoResearch #715-545-150, 1:500), Alexa Fluor ® 594 anti-Rabbit IgG (Jackson ImmunoResearch #711-585-152, 1:500).

    Techniques: Western Blot, Expressing

    The import of peroxisomal matrix enzymes declines during B cell aging. (A) Immunofluorescence staining of SKL and PEX14 in CD19 + B cells isolated from young (23-year old) and old individuals (74-year old). Hoechst 33342 was used for nuclear staining. Scale bar: 2 μm. (B) Pearson correlation coefficient ( r ) to quantify SKL and PEX14 co-localization in four immune cell types with age. (C) Quantification of SKL puncta number in four immune cell types with age. (D) Quantification of SKL fluorescence intensity in four immune cell types with age. Student’s t -test, n = 7. Data are represented as mean ± SD. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).

    Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

    Article Title: Aging impairs peroxisome biogenesis in human B cells

    doi: 10.1093/gerona/glaf148

    Figure Lengend Snippet: The import of peroxisomal matrix enzymes declines during B cell aging. (A) Immunofluorescence staining of SKL and PEX14 in CD19 + B cells isolated from young (23-year old) and old individuals (74-year old). Hoechst 33342 was used for nuclear staining. Scale bar: 2 μm. (B) Pearson correlation coefficient ( r ) to quantify SKL and PEX14 co-localization in four immune cell types with age. (C) Quantification of SKL puncta number in four immune cell types with age. (D) Quantification of SKL fluorescence intensity in four immune cell types with age. Student’s t -test, n = 7. Data are represented as mean ± SD. Each data point (circle in the graphs) represents an individual participant, categorized into two age groups: young (19- to 35-year old) and old (60- to 74-year old).

    Article Snippet: The primary and secondary antibodies are summarized below: anti-CD19 (Proteintech #66298-1-Ig, 1:200), anti-CD14 (Proteintech #60253-1-Ig, 1:200), anti-CD4 (Proteintech #67786-1-Ig, 1:200), anti-CD8 (Proteintech #66868-1-Ig, 1:200), anti-SKL (Gift from Richard Rachubinski lab, 1:200), CoraLite ® 594-conjugated PEX14 Polyclonal antibody (Proteintech # CL594-10594, 1:200), Alexa Flour ® 488 anti-mouse IgG (Jackson ImmunoResearch #715-545-150, 1:500), Alexa Fluor ® 594 anti-Rabbit IgG (Jackson ImmunoResearch #711-585-152, 1:500).

    Techniques: Immunofluorescence, Staining, Isolation, Fluorescence