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pet14b vector  (Millipore)


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    Structured Review

    Millipore pet14b vector
    Pet14b Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet14b/pet19b/us12031143-1364-9-11
    Average 90 stars, based on 1 article reviews
    pet14b vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Insecticidal proteins from plants and methods for their use
    Article Snippet: The IPD110Ca gene (SEQ ID NO: 3) and IPD110Ea gene (SEQ ID NO: 8) sequences were synthesized as gBlocksTM (IDT, Coralville, Iowa).

    Amplification:

    Article Title: A dynamic interplay between chitin synthase and the proteins Expansion/Rebuf reveals that chitin polymerisation and translocation are uncoupled in Drosophila
    Article Snippet: .. The amplified fragments were cloned into the expression vector pET14b (Novagen). .. The resulting positive clones were used to transform BL2 (C41) cells (Novagen) for protein expression.

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation.
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His6 tag followed by a TEV cleavage sequence. ..

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His 6 tag followed by a TEV cleavage sequence. ..

    Clone Assay:

    Article Title: A dynamic interplay between chitin synthase and the proteins Expansion/Rebuf reveals that chitin polymerisation and translocation are uncoupled in Drosophila
    Article Snippet: .. The amplified fragments were cloned into the expression vector pET14b (Novagen). .. The resulting positive clones were used to transform BL2 (C41) cells (Novagen) for protein expression.

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation.
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His6 tag followed by a TEV cleavage sequence. ..

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His 6 tag followed by a TEV cleavage sequence. ..

    Article Title: StpA protein from Escherichia coli condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI
    Article Snippet: .. A cDNA insert corresponding to the stpA gene from E.coli (SwissProt primary accession number P30017; kindly provided by J. C. D. Hinton) was cloned previously in the expression vector pET14b (Novagen) between the 5′ Nde1 site and the 3′ BamH1 site. ..

    Expressing:

    Article Title: A dynamic interplay between chitin synthase and the proteins Expansion/Rebuf reveals that chitin polymerisation and translocation are uncoupled in Drosophila
    Article Snippet: .. The amplified fragments were cloned into the expression vector pET14b (Novagen). .. The resulting positive clones were used to transform BL2 (C41) cells (Novagen) for protein expression.

    Article Title: StpA protein from Escherichia coli condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI
    Article Snippet: .. A cDNA insert corresponding to the stpA gene from E.coli (SwissProt primary accession number P30017; kindly provided by J. C. D. Hinton) was cloned previously in the expression vector pET14b (Novagen) between the 5′ Nde1 site and the 3′ BamH1 site. ..

    Plasmid Preparation:

    Article Title: A dynamic interplay between chitin synthase and the proteins Expansion/Rebuf reveals that chitin polymerisation and translocation are uncoupled in Drosophila
    Article Snippet: .. The amplified fragments were cloned into the expression vector pET14b (Novagen). .. The resulting positive clones were used to transform BL2 (C41) cells (Novagen) for protein expression.

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation.
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His6 tag followed by a TEV cleavage sequence. ..

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His 6 tag followed by a TEV cleavage sequence. ..

    Article Title: Combinations of insecticidal polypeptides having improved activity spectrum and uses thereof
    Article Snippet: The PCR products were digested with NdeI/XhoI (New England Biolabs, Ipswich, MA) and ligated into a pET14b (Novagen) plasmid digested by the same enzymes. .. The PCR products were digested with NdeI/XhoI (New England Biolabs, Ipswich, MA) and ligated into a pET14b (Novagen) plasmid digested by the same enzymes. ..

    Article Title: StpA protein from Escherichia coli condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI
    Article Snippet: .. A cDNA insert corresponding to the stpA gene from E.coli (SwissProt primary accession number P30017; kindly provided by J. C. D. Hinton) was cloned previously in the expression vector pET14b (Novagen) between the 5′ Nde1 site and the 3′ BamH1 site. ..

    Polymerase Chain Reaction:

    Article Title: A Novel Pathway for the Biosynthesis of Heme in Archaea : Genome-Based Bioinformatic Predictions and Experimental Evidence
    Article Snippet: .. The resulting PCR fragments and the vector pET14b (Novagen) were subsequently digested with Nde I and Bam HI and ligated to generate pET14b_ mba_A1791 . ..

    Article Title: Combinations of insecticidal polypeptides having improved activity spectrum and uses thereof
    Article Snippet: The PCR products were digested with NdeI/XhoI (New England Biolabs, Ipswich, MA) and ligated into a pET14b (Novagen) plasmid digested by the same enzymes. .. The PCR products were digested with NdeI/XhoI (New England Biolabs, Ipswich, MA) and ligated into a pET14b (Novagen) plasmid digested by the same enzymes. ..

    Filtration:

    Article Title: Enzyme Sequence and Its Relationship to Hyperbaric Stability of Artificial and Natural Fish Lactate Dehydrogenases
    Article Snippet: Other materials were provided by the following suppliers: SMART RACETM cDNA Amplification kit and Advantage 2 PCR System were from CLONTECH, Mountain View, California, USA. .. ; restriction enzymes were from Promega, Chilworth, Southampton, U.K.; chelating Sepharose fast flow resin and gel filtration columns were from GE Healthcare, Little Chalfont, Bucks, U.K.; pET14b was from Novagen, Madison, WI, USA; pMalC2 and amylose resin were from New England Biolabs, Beverly, Maine, USA: all RNA preservation systems were from AMBION, Austin, Texas, USA; RNA extraction kits were from QIAGEN, Crawley, West Sussex, UK; and primers were from Invitrogen, Paisley, UK. ..

    Preserving:

    Article Title: Enzyme Sequence and Its Relationship to Hyperbaric Stability of Artificial and Natural Fish Lactate Dehydrogenases
    Article Snippet: Other materials were provided by the following suppliers: SMART RACETM cDNA Amplification kit and Advantage 2 PCR System were from CLONTECH, Mountain View, California, USA. .. ; restriction enzymes were from Promega, Chilworth, Southampton, U.K.; chelating Sepharose fast flow resin and gel filtration columns were from GE Healthcare, Little Chalfont, Bucks, U.K.; pET14b was from Novagen, Madison, WI, USA; pMalC2 and amylose resin were from New England Biolabs, Beverly, Maine, USA: all RNA preservation systems were from AMBION, Austin, Texas, USA; RNA extraction kits were from QIAGEN, Crawley, West Sussex, UK; and primers were from Invitrogen, Paisley, UK. ..

    RNA Extraction:

    Article Title: Enzyme Sequence and Its Relationship to Hyperbaric Stability of Artificial and Natural Fish Lactate Dehydrogenases
    Article Snippet: Other materials were provided by the following suppliers: SMART RACETM cDNA Amplification kit and Advantage 2 PCR System were from CLONTECH, Mountain View, California, USA. .. ; restriction enzymes were from Promega, Chilworth, Southampton, U.K.; chelating Sepharose fast flow resin and gel filtration columns were from GE Healthcare, Little Chalfont, Bucks, U.K.; pET14b was from Novagen, Madison, WI, USA; pMalC2 and amylose resin were from New England Biolabs, Beverly, Maine, USA: all RNA preservation systems were from AMBION, Austin, Texas, USA; RNA extraction kits were from QIAGEN, Crawley, West Sussex, UK; and primers were from Invitrogen, Paisley, UK. ..

    cDNA Library Assay:

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation.
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His6 tag followed by a TEV cleavage sequence. ..

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His 6 tag followed by a TEV cleavage sequence. ..

    Modification:

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation.
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His6 tag followed by a TEV cleavage sequence. ..

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His 6 tag followed by a TEV cleavage sequence. ..

    Sequencing:

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation.
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His6 tag followed by a TEV cleavage sequence. ..

    Article Title: Structure determination and analysis of titin A-band fibronectin type III domains provides insights for disease-linked variants and protein oligomerisation
    Article Snippet: .. DNA sequences encoding the wild type (WT) titin domains Fn3-3, Fn3-11, Fn3-20, Fn3-49, Fn3-56, Fn3-85 and Fn3-90 were amplified from a human skeletal muscle cDNA library and cloned into a vector modified from pET14b (Millipore) containing an N-terminal His 6 tag followed by a TEV cleavage sequence. ..



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