4 peg2 azide (MedChemExpress)

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MedChemExpress 4 peg2 azide
4 Peg2 Azide, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peg2/Pomalidomide+4'-PEG2-azide/10__1038_slash_s41598___026___74569___2-249-6-22
Average 94 stars, based on 1 article reviews

4 peg2 azide - by Bioz Stars, 2026-11

94/100 stars

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Incubation:

Article Title: eIF4E and Ezrin cooperate in pseudopods to drive a localized migratory translation program in acute myeloid leukemia
Article Snippet: Cells were then washed three times with RL buffer and blocked for 30 minutes at RT with 2% BSA with 2mg/ml Biotin (cat# B1595, Invitrogen) in RL buffer. .. After washing three times with RL buffer, cells were incubated for 1 hour at RT with 50mM Biotinylated Puromycin (cat# 2379889-82-2, MedChemExpress) in RL buffer with 2% BSA. .. After washing three times with RL buffer, cells were incubated with 2mg/ml NeutrAvidin Oregon Green 488 (cat# A6374, Invitrogen) in 2% BSA in RL buffer for 1 hour at RT, washed three times with RL buffer and either mounted in ProLong TM gold antifade media with DAPI, or stained for proteins as indicated in the text.

Article Title: Covalently targeting HSP90 with a natural small-molecule costunolide to inhibit necroptosis and ulcerative colitis.
Article Snippet: Background: Necroptosis exacerbates ulcerative colitis (UC) by disrupting intestinal barriers and promoting necroinflammation.. Heat shock protein 90 (HSP90) regulates receptor-interacting protein kinase 1/3 (RIPK1/3) activity through its chaperone function, thereby participating in necroptosis and emerging as a potential therapeutic target for its inhibition in UC therapy.. Costunolide (CTL), a principal sesquiterpene lactone from Aucklandia lappa, has poorly defined mechanisms in UC treatment.

Protein Concentration:

CTL Assay:

Generated:

Article Title: Intracellular sclerostin promotes tumor progression and metastasis as a potential therapeutic target in triple-negative breast cancer.
Article Snippet: An H-score > 50 was used as the threshold for sclerostin positivity.34 Preparation of sclerostin aptamer-based PROTACs In the plastic reaction tube, 5′-alkyne sclerostin aptamer Apc001OA or Cy3-labled Apc001OA (1.0 eq),18 azide linked-VHL/CRBN/ MDM2 ligand ((S, R, S)-AHPC-PEG4-N3, MedChemExpress, Cat: HY-103601) (Thalidomide-O-PEG4-azide, MedChemExpress, Cat: HY-140844) (Nutlin-C1-amido-PEG4-C2-N3, MedChemExpress, Cat: HY-128832) or azide linked-VHL ligands with different linkers (100 eq), and Copper (II) sulfate pentahydrate (Sigma-AIdrich, Cat: 469130) (50 eq) were transferred. .. For the azide linkedVHL ligands with different linkers, which were generated by mixing the azide-linker-NHS esters (N3-PEG2-C2-NHS ester, leyan, Cat: 1175728) (N3-PEG4-CH2COONHS, ACHEMBLOCK, Cat: V156175) (Azido-PEG6-NHS ester, BROADPHARM, Cat: BP21506) (Azido-PEG8-NHS ester, BROADPHARM, Cat: BP-215606) (Azido-PEG10-NHS ester, BROADPHARM, Cat: BP-23998) (1.0 eq), NH2-VHL ligand ((S, R, S)-AHPC, MedChemExpress, Cat: HY-125845) ((2S,4S)-1-((S)-2-Amino-3,3-dimethylbutanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl) benzyl)pyrrolidine-2-carboxamide hydrochloride, bidepharm, Cat: BD01272772) (1.5 eq), and N,NDiisopropylethylamine (MACKLIN, Cat: N741860) (1.5 eq) in DMSO for 4 h, respectively. ..

Article Title: Intracellular sclerostin promotes tumor progression and metastasis as a potential therapeutic target in triple-negative breast cancer
Article Snippet: After blocking (Beyotime, Cat: P0260), the sections were incubated overnight at 4°C with the primary antibodies against sclerostin (Thermo Fisher Scientific, Cat: PA5-113315; 1:500) or Ki-67 (Proteintech, Cat: 27309-1-AP; 1:500), followed by horseradish peroxidase (HRP)-conjugated secondary antibodies (Gene Tech, Cat: GK500710). .. For the azide linked-VHL ligands with different linkers, which were generated by mixing the azide-linker-NHS esters (N3-PEG2-C2-NHS ester, leyan, Cat: 1175728) (N3-PEG4-CH2COONHS, ACHEMBLOCK, Cat: V156175) (Azido-PEG6-NHS ester, BROADPHARM, Cat: BP-21506) (Azido-PEG8-NHS ester, BROADPHARM, Cat: BP-215606) (Azido-PEG10-NHS ester, BROADPHARM, Cat: BP-23998) (1.0 eq), NH 2 -VHL ligand ((S, R, S)-AHPC, MedChemExpress, Cat: HY-125845) ((2S,4S)-1-((S)-2-Amino-3,3-dimethylbutanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl) benzyl)pyrrolidine-2-carboxamide hydrochloride, bidepharm, Cat: BD01272772) (1.5 eq), and N,N-Diisopropylethylamine (MACKLIN, Cat: N741860) (1.5 eq) in DMSO for 4 h, respectively. ..

Magnetic Beads:

Article Title: Spatiotemporally controlled ferritin-targeting PROTAC nanoplatform for visualized manipulation of endogenous “Iron pool” in pancreatic cancer therapy
Article Snippet: Ammonium fluoride (NH 4 F) and 4-toluene sulfonic acid were obtained from Macklin (Shanghai, China). .. Fluorescamine, H 2 N-PEG 2 -CH 2 COOH, (S,R,S)-AHPC-Me hydrochloride (VHL ligand), fluorescein isothiocyanate (FITC), 1,3-diphenylisobenzofuran (DPBF), BODIPY581/591-C11, FerroOrange, MG132 and Protein A/G IP Magnetic Beads were purchased from MedChemExpress LLC (Shanghai, China). .. Dichloromethane (DCM), N,N -dimethylformamide (DMF) were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).

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