anti pde4a  (Proteintech)

Journal: International Journal of Neuropsychopharmacology

Article Title: Upregulation of Phosphodiesterase 7A Contributes to Concurrent Pain and Depression via Inhibition of cAMP-PKA-CREB-BDNF Signaling and Neuroinflammation in the Hippocampus of Mice

doi: 10.1093/ijnp/pyae040

Figure Lengend Snippet: Partial sciatic nerve ligation (PSNL) or complete Freund’s adjuvant (CFA) leads to increased phosphodiesterase 4B (PDE4B) and PDE7A and decreased cyclic AMP (cAMP) expression in mouse hippocampus. Mice were treated as described in the schedule of . The expression of PDE2, PDE4, PDE5, and PDE7 in the hippocampus of mice subjected to PSNL or CFA were quantified by western blotting analysis (A–G), and the levels of cAMP and cyclic GMP (cGMP) were analyzed by ELISA assays (H–I) 33 days after surgery or injection. The optic densities of PDE2A, PDE4A, PDE4B, PDE4D, PDE5A, PDE7A, and PDE7B were normalized to those of sham (or saline) controls. Data shown are mean ± SEM (n = 5). * P < .05, ** P < .01, *** P < .001 vs corresponding sham or saline.

Article Snippet: The membrane was incubated overnight at 4°C with primary antibodies (diluted 1:1000) against the following proteins: PDE2A (Proteintech, Chicago, IL, USA), PDE4A (Proteintech), PDE4B (Cell Signaling Technology, Danvers, MA, USA), PDE4D (Abcam, Cambridge, UK), PDE5A (Abcam), PDE7A (Santa Cruz Biotechnology, Shanghai, China), PDE7B (Abcam), total PKA or phosphorylated PKA (p-PKA; Cell Signaling Technology), total CREB or phosphorylated CREB (p-CREB; Abcam), BDNF (Abcam), the p65 subunit of NF-kB (Cell Signaling Technology), or β-actin (Zsbio, Beijing, China).

Techniques: Ligation, Adjuvant, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Injection, Saline

Journal: International Journal of Neuropsychopharmacology

Article Title: Identification of Phosphodiesterase-7A (PDE7A) as a Novel Target for Reducing Ethanol Consumption in Mice

doi: 10.1093/ijnp/pyae032

Figure Lengend Snippet: PDE7A expression was upregulated following ethanol administration in female mice. (A–C) Protein expression of PDE isoforms, including PDE1B, PDE2A, PDE4A, PDE4B, PDE4D, PDE7A, PDE7B, PDE9A, and PDE10A, in the hippocampus, prefrontal cortex, and striatum of C57BL/6J female mice after saline or EtOH (i.p., 2.5 g/kg) injection for 7 days. The upper image shows the true bands of PDE7A and GAPDH at their respective KDa quantitative value ( t test, PDE7A: EtOH vs saline, **** P < .0001). (D–F) Expression of PDE isoforms in male mice after EtOH injection. The upper image shows the true bands of PDE7A and GAPDH at their respective KDa quantitative value ( t test, PDE7A: EtOH vs saline, P > .05). N = 6 mice for each group. All data shown are means ± SEM. The ratio of PDE7A to the respective GAPDH in each lane was calculated for statistical analysis.

Article Snippet: The antibodies against PDE1B (Cat# ab182565, 1:1000), PDE2A (Cat# ab140650, 1:1000), PDE4A (Cat# ab200383, 1:1000), PDE4B (Cat# ab170939, 1:1000), PDE4D (Cat# ab171750, 1:1000), PDE7B (Cat# ab170914, 1:1000), PDE10A (Cat# ab227829, 1:1000), or PKA (Cat# ab32514, 1:1000) were purchased from Abcam (Cambridge, UK).

Techniques: Expressing, Saline, Injection