pd90780  (MedChemExpress)

Journal: Journal of Advanced Research

Article Title: Astragaloside II, a natural saponin, facilitates remyelination in demyelination neurological diseases via p75NTR receptor mediated β-catenin/Id2/MBP signaling axis in oligodendrocyte precursor cells

doi: 10.1016/j.jare.2025.04.028

Figure Lengend Snippet: Affinity studies between AS-II and its target p75NTR . (A, B) Coomassie Blue staining and MS-based DARTs sequencing were conducted to identify the target proteins of AS-II. (C) Molecular docking visualization of AS-II binding to p75NTR complex structure. (D) Molecular docking results for p75NTR (PDB: 5ZGG) with AS-II, NSC49652, and K252a. (E) Antiproteolytic effect of p75NTR protein in combination with AS-II (n = 4). (F) Effects of varying concentrations of AS-II on its binding and protease resistance to p75NTR (n = 4). (G) Thermal stability of p75NTR with or without AS-II (n = 4). (H) Binding affinities of AS-II and K252a (at concentrations from 1.5625-100 μM), PD90780 (at concentrations from 1.5625-200 μM) to p75NTR were determined using SPR assay. (I) Predicted binding sites of AS-II to p75NTR. (J) Effects of AS-II (100 μM) on the expression of GSK3β Tyr216 , β-catenin, and MBP in MO3.13 cells transfected with WT or mutant p75NTR (n = 4). Data are presented as mean ± SD; & p < 0.05, && p < 0.01, vs GAPDH band; # p < 0.05, ### p < 0.001, vs Ctrl group; * p < 0.05, ** p < 0.01, vs DMSO group. ns, not significant. Statistical test: Two-group comparisons used Student's t -test (E, G, J), multi-group comparisons used one-way ANOVA with Dunnett's test (F). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: 3,3′,5-Triiodo-L-thyronine (T3, Cat. No. HY-A0070), SKL2001 (Cat. No. HY-101085), PD90780 (Cat. No. HY-110166 ), and K252a (Cat. No. HY-N6732) were obtained from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Staining, Sequencing, Binding Assay, SPR Assay, Expressing, Transfection, Mutagenesis

Journal: Experimental & Molecular Medicine

Article Title: Nerve growth factor upregulates sirtuin 1 expression in cholestasis: a potential therapeutic target

doi: 10.1038/emm.2017.235

Figure Lengend Snippet: Effects of TrkA and p75 NTR blocker treatments on bile duct ligation (BDL)-induced mouse liver injury. The ICR mice receiving BDL surgery were intraperitoneally administered either recombinant NGF ( n =6) twice weekly or PBS ( n =6), GW441756 (GW; n =6), DMSO ( n =3) or PD90780 (PD; n =6) daily. At 14 days postoperation, serum levels of AST ( a ), ALT ( b ) and total bilirubin ( c ) were biochemically measured, whereas tumor necrosis factor (TNF)-α ( d ) and interleukin (IL)-6 ( e ) levels in serum were analyzed by ELISA. The H&E staining results of mice with BDL-induced cholestatic injury or simultaneous treatments show the hepatic necrotic regions in dashed circles ( f ), quantified by morphometric analysis ( g ). Scale bar=1000 μm. All of the data are shown as the mean±s.e.m. ND, not detectable. NS, not significant. * P <0.05 between the indicated groups.

Article Snippet: A p75NTR blocker, PD90780, was purchased from Axon Medchem (Groningen, The Netherlands).

Techniques: Ligation, Recombinant, Enzyme-linked Immunosorbent Assay, Staining

Journal: Experimental & Molecular Medicine

Article Title: Nerve growth factor upregulates sirtuin 1 expression in cholestasis: a potential therapeutic target

doi: 10.1038/emm.2017.235

Figure Lengend Snippet: Effects of TrkA and p75 NTR blocker treatments on bile duct ligation (BDL)-induced PARP cleavage and apoptosis of parenchymal hepatocytes. The mice receiving BDL surgery were intraperitoneally administered with either recombinant NGF ( n =6) twice weekly or PBS ( n =6), GW441756 (GW; n =6), DMSO ( n =3) or PD90780 (PD; n =6) daily. After 2 weeks of treatment, pooled liver extracts of each group were subjected to western blotting to detect both propeptides and cleaved forms of caspase-3 and PARP ( a ). Density analysis of the activation of caspase-3 ( b ) and PARP ( c ), shown as the ratios of cleavage to propeptide content, followed by normalization to negative control (NC) levels. IHC staining for active PARP peptides ( d ) and labeling index ( e ) quantitatively demonstrated that PD90780 administration potentiated the increase of PARP activation in BDL-treated mouse livers. ( f ) Representative microphotographs of TUNEL staining showing the apoptotic events in mouse liver sections. Scale bars=50 μm. ( g ) Analysis of TUNEL-positive parenchymal cells indicated that recombinant NGF administration exerted a protective effect, whereas treatment with a p75NTR antagonist, PD90780, increased apoptotic cell death in BDL-induced cholestatic injured livers. All data are shown as the mean±s.e.m. * P <0.05 between indicated groups. ND, not detectable. NS, not significant.

Article Snippet: A p75NTR blocker, PD90780, was purchased from Axon Medchem (Groningen, The Netherlands).

Techniques: Ligation, Recombinant, Western Blot, Activation Assay, Negative Control, Immunohistochemistry, Labeling, TUNEL Assay, Staining

Journal: Experimental & Molecular Medicine

Article Title: Nerve growth factor upregulates sirtuin 1 expression in cholestasis: a potential therapeutic target

doi: 10.1038/emm.2017.235

Figure Lengend Snippet: Effects of simultaneous administration with NGF and its receptor blockers on bile duct ligation (BDL)-induced mouse liver injury. The ICR mice receiving BDL surgery were intraperitoneally administered recombinant NGF (1 mg kg −1 ; n =3) twice weekly and simultaneous daily injections of PBS ( n =6), GW441756 (GW; 1 mg kg −1 ; n =6), DMSO ( n =6) or PD90780 (PD; 1 mg kg −1 ; n =6). At 14 days postoperation, serum levels of AST ( a ) and total bilirubin ( b ) were biochemically measured, whereas serum interleukin (IL)-6 levels ( c ) were analyzed by ELISA. ( d ) The representative H&E staining results showing the necrotic regions in cholestasis-injured livers. Scale bar=1000 μm. ( e ) Morphometric analysis of necrotic region areas. All data are shown as the mean±s.e.m. * P <0.05 between the indicated groups. ND, not detectable. NS, not significant.

Article Snippet: A p75NTR blocker, PD90780, was purchased from Axon Medchem (Groningen, The Netherlands).

Techniques: Ligation, Recombinant, Enzyme-linked Immunosorbent Assay, Staining

Journal: Experimental & Molecular Medicine

Article Title: Nerve growth factor upregulates sirtuin 1 expression in cholestasis: a potential therapeutic target

doi: 10.1038/emm.2017.235

Figure Lengend Snippet: Effects of simultaneous administration with NGF and its receptor blockers on bile duct ligation (BDL)-induced apoptosis of parenchymal hepatocytes. The ICR mice receiving BDL surgery were intraperitoneally administered recombinant NGF (1 mg kg −1 ; n =3) twice weekly and simultaneous daily injections of PBS ( n =6), GW441756 (GW; 1 mg kg −1 ; n =6), DMSO ( n =6) or PD90780 (PD; 1 mg kg −1 ; n =6). After 2 weeks of treatment, pooled liver extracts of each group were subjected to western blotting to detect both propeptides and cleaved forms of caspase-3 and PARP ( a ). The activation of caspase-3 ( b ) and PARP ( c ) was densitometrically analyzed and is shown as the cleavage-to-propeptide ratios and normalized to negative control (NC) levels. ( d ) Representative images of IHC staining for activated PARP in the mouse livers. ( e ) Quantitative measurement of PARP activation as shown by labeling index. ( f ) Representative microphotographs of TUNEL staining showing the apoptotic events in mouse liver sections. Scale bars=50 μm. ( g ) Analysis of TUNEL-positive parenchymal cells indicated that GW treatment significantly increased apoptotic cell death in BDL-induced cholestatic injured livers. All data are shown as the mean±s.e.m. * P <0.05 between indicated groups. ND, not detectable. NS, not significant.

Article Snippet: A p75NTR blocker, PD90780, was purchased from Axon Medchem (Groningen, The Netherlands).

Techniques: Ligation, Recombinant, Western Blot, Activation Assay, Negative Control, Immunohistochemistry, Labeling, TUNEL Assay, Staining

Journal: Experimental & Molecular Medicine

Article Title: Nerve growth factor upregulates sirtuin 1 expression in cholestasis: a potential therapeutic target

doi: 10.1038/emm.2017.235

Figure Lengend Snippet: Involvement of NGF-induced SIRT1 upregulation in hepatocytes under oxidative stress. ( a ) Huh-7 hepatocytes were treated with either NGF or proNGF for 24 h. Western blotting detection indicated that NGF, but not proNGF, upregulated SIRT1 expression in human hepatocytes. ( b ) SIRT1 upregulation by NGF stimulation in cultured rat and mouse primary hepatocytes. ( c ) Kinetic profiles of NGF-mediated signaling activation. Huh-7 cells were treated with NGF for the indicated durations and measured by western blotting. ( d ) Involvement of NF-κB and SIRT1 activities in the NGF-induced SIRT1 upregulation. Huh-7 cells were pretreated with 10 μ M PDTC or 20 μM EX-527 for 2 h, followed by 2 or 24 h of NGF stimulation. ( e ) Effects of NGFR blockers, kinase inhibitors and SIRT1 modulators on oxidative stress-activated pro-apoptotic machinery. Huh-7 cells were pretreated either with 20 μ M resveratrol (RSV; a SIRT1 agonist) or with 10 ng ml −1 NGF for 24 h in the presence of 10 μ M NGFR blockers (GW441756 and PD90780), 10 μ M kinase blockers (PDTC, LY294002 and PD98059), 20 μ M EX-527 (a SIRT1 antagonist) or equivalent DMSO, followed by 1-h transient exposure to 0.8 m M H 2 O 2 . After H 2 O 2 removal and another 4-h incubation, protein lysates were collected for western blotting detection of caspase-3 and PARP. The density analysis data are shown in . ( f ) Effects of NGFR blockers, kinase inhibitors and SIRT1 modulators on oxidative stress-induced hepatotoxicity as measured by MTT-based cell viability assay. All data are shown as the mean±s.d. of three independent experiments. * P <0.05 vs DMSO solvent control or between the indicated groups; # P <0.05 vs the H 2 O 2 treatment group.

Article Snippet: A p75NTR blocker, PD90780, was purchased from Axon Medchem (Groningen, The Netherlands).

Techniques: Western Blot, Expressing, Cell Culture, Activation Assay, Incubation, Viability Assay, Solvent, Control

Journal:

Article Title: Role of p75 NTR in female rat urinary bladder with cyclophosphamide-induced cystitis

doi: 10.1152/ajprenal.90501.2008

Figure Lengend Snippet: Table 2.

Article Snippet: PD90780 was obtained through a compound transfer agreement with Pfizer (Groton, CT).

Techniques: