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ps100010  (OriGene)


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    Structured Review

    OriGene ps100010
    Ps100010, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 551 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv6/pCMV6-AC-GFP+Mammalian+Expression+Vector/pmc13233774-56-8-5
    Average 96 stars, based on 551 article reviews
    ps100010 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: hnRNPL phase separation activates PIK3CB transcription and promotes glycolysis in ovarian cancer.
    Article Snippet: Chemicals used in this study include: PI-103 (S1038, Selleckchem), TGX-221 (S1169, Selleckchem), ATP assay kit (A095-2-1, Nanjing Jiancheng Bioengineering Institute), Glucose content test kit (ADS-WTDX002, AIDISHENG), Lactic acid content test kit (ADS-W-T009-96, AIDISHENG), TransDetect Double-Luciferase Reporter Assay Kit (FR201-02, TransGen), Versatile DNA/siRNA transfection reagent (101000046, Polyplus), Seahorse XFe24 FluxPak mini (102342-100, Agilent), Seahorse XF Glycolysis Stress Test Kit (103020-100, Agilent), ClonExpress MultiS One Step Cloning Kit (C113, Vazyme), TIANprep midi Plasmid kit (DP106, TIANGEN), TIANamp Genomic DNA kit (DP304, TIANGEN), Magna ChIP A/G Chromatin Immunoprecipitation kit (9003, CST). .. pLKO.1 (#10878), psPAX2 (#12260) and pMD2.G (#12259) plasmid vectors were purchased from Addgene Company. pCMV6 plasmid vector was purchased from OriGene. ..

    Article Title: MAP4K2 suppresses antitumor immunity in a pancreatic cancer model by promoting Treg differentiation
    Article Snippet: The plasmid expressing 3xFlag-tagged human DDX39B protein was generated by subcloning DDX39B cDNA into the vector pCMV6-AN-3DDK (OriGene). .. The plasmids expressing CFP-fused MAP4K2 and YFP-fused DDX39B proteins were constructed by subcloning the MAP4K2 cDNA and DDX39B cDNA from the 3xMyc-MAP4K2 plasmid and 3xFlag-DDX39B plasmid into the pCMV6-AC-CFP vector (OriGene) and pCMV6-AN-YFP vector (OriGene), respectively. ..

    Article Title: MAP4K2 suppresses antitumor immunity in a pancreatic cancer model by promoting Treg differentiation
    Article Snippet: The plasmid expressing 3xFlag-tagged human DDX39B protein was generated by subcloning DDX39B cDNA into the vector pCMV6-AN-3DDK (OriGene). .. The plasmids expressing CFP-fused MAP4K2 and YFP-fused DDX39B proteins were constructed by subcloning the MAP4K2 cDNA and DDX39B cDNA from the 3xMyc- MAP4K2 plasmid and 3xFlag- DDX39B plasmid into the pCMV6-AC-CFP vector (OriGene) and pCMV6-AN-YFP vector (OriGene), respectively. ..

    Expressing:

    Article Title: Discovery of an Endonuclease G-inhibitory Ku80-peptide protecting against leukemogenic rearrangements at the MLL breakpoint cluster
    Article Snippet: For co-treatment with doxorubicin and DNA-PK inhibitor, HeLa reporter cells were treated with either H2O or 1 μM NU7441 (STEMCELL Technologies, Vancouver, Canada) for 1 h before being exposed to H2O or 0.5 μM doxorubicin in H2O for 4 h. .. For analysis of Ku80-wt, Ku80-Ct or Ku80(E720A, E721A) the following expression plasmids with a pCMV6-AN-DDK backbone were generated (OriGene, Rockville, Maryland, USA): pCMV6-AN-DDK-Ku80 expressing Ku80-wt (SC112985 ORF, OriGene) with N-terminal Flag-tag and pCMV6-AN-DDK-Ku80 C-terminus expressing Ku80-Ct through fusion of the Ku80-NLS motif P562-K568 (encoded by 5 ́-CCTACAGCTAAAAAATTAAAG-3 ́). ..

    Article Title: MAP4K2 suppresses antitumor immunity in a pancreatic cancer model by promoting Treg differentiation
    Article Snippet: The plasmid expressing 3xFlag-tagged human DDX39B protein was generated by subcloning DDX39B cDNA into the vector pCMV6-AN-3DDK (OriGene). .. The plasmids expressing CFP-fused MAP4K2 and YFP-fused DDX39B proteins were constructed by subcloning the MAP4K2 cDNA and DDX39B cDNA from the 3xMyc-MAP4K2 plasmid and 3xFlag-DDX39B plasmid into the pCMV6-AC-CFP vector (OriGene) and pCMV6-AN-YFP vector (OriGene), respectively. ..

    Article Title: MAP4K2 suppresses antitumor immunity in a pancreatic cancer model by promoting Treg differentiation
    Article Snippet: The plasmid expressing 3xFlag-tagged human DDX39B protein was generated by subcloning DDX39B cDNA into the vector pCMV6-AN-3DDK (OriGene). .. The plasmids expressing CFP-fused MAP4K2 and YFP-fused DDX39B proteins were constructed by subcloning the MAP4K2 cDNA and DDX39B cDNA from the 3xMyc- MAP4K2 plasmid and 3xFlag- DDX39B plasmid into the pCMV6-AC-CFP vector (OriGene) and pCMV6-AN-YFP vector (OriGene), respectively. ..

    Generated:

    Article Title: Discovery of an Endonuclease G-inhibitory Ku80-peptide protecting against leukemogenic rearrangements at the MLL breakpoint cluster
    Article Snippet: For co-treatment with doxorubicin and DNA-PK inhibitor, HeLa reporter cells were treated with either H2O or 1 μM NU7441 (STEMCELL Technologies, Vancouver, Canada) for 1 h before being exposed to H2O or 0.5 μM doxorubicin in H2O for 4 h. .. For analysis of Ku80-wt, Ku80-Ct or Ku80(E720A, E721A) the following expression plasmids with a pCMV6-AN-DDK backbone were generated (OriGene, Rockville, Maryland, USA): pCMV6-AN-DDK-Ku80 expressing Ku80-wt (SC112985 ORF, OriGene) with N-terminal Flag-tag and pCMV6-AN-DDK-Ku80 C-terminus expressing Ku80-Ct through fusion of the Ku80-NLS motif P562-K568 (encoded by 5 ́-CCTACAGCTAAAAAATTAAAG-3 ́). ..

    Construct:

    Article Title: MAP4K2 suppresses antitumor immunity in a pancreatic cancer model by promoting Treg differentiation
    Article Snippet: The plasmid expressing 3xFlag-tagged human DDX39B protein was generated by subcloning DDX39B cDNA into the vector pCMV6-AN-3DDK (OriGene). .. The plasmids expressing CFP-fused MAP4K2 and YFP-fused DDX39B proteins were constructed by subcloning the MAP4K2 cDNA and DDX39B cDNA from the 3xMyc-MAP4K2 plasmid and 3xFlag-DDX39B plasmid into the pCMV6-AC-CFP vector (OriGene) and pCMV6-AN-YFP vector (OriGene), respectively. ..

    Article Title: MAP4K2 suppresses antitumor immunity in a pancreatic cancer model by promoting Treg differentiation
    Article Snippet: The plasmid expressing 3xFlag-tagged human DDX39B protein was generated by subcloning DDX39B cDNA into the vector pCMV6-AN-3DDK (OriGene). .. The plasmids expressing CFP-fused MAP4K2 and YFP-fused DDX39B proteins were constructed by subcloning the MAP4K2 cDNA and DDX39B cDNA from the 3xMyc- MAP4K2 plasmid and 3xFlag- DDX39B plasmid into the pCMV6-AC-CFP vector (OriGene) and pCMV6-AN-YFP vector (OriGene), respectively. ..

    Subcloning:

    Article Title: MAP4K2 suppresses antitumor immunity in a pancreatic cancer model by promoting Treg differentiation
    Article Snippet: The plasmid expressing 3xFlag-tagged human DDX39B protein was generated by subcloning DDX39B cDNA into the vector pCMV6-AN-3DDK (OriGene). .. The plasmids expressing CFP-fused MAP4K2 and YFP-fused DDX39B proteins were constructed by subcloning the MAP4K2 cDNA and DDX39B cDNA from the 3xMyc-MAP4K2 plasmid and 3xFlag-DDX39B plasmid into the pCMV6-AC-CFP vector (OriGene) and pCMV6-AN-YFP vector (OriGene), respectively. ..

    Article Title: MAP4K2 suppresses antitumor immunity in a pancreatic cancer model by promoting Treg differentiation
    Article Snippet: The plasmid expressing 3xFlag-tagged human DDX39B protein was generated by subcloning DDX39B cDNA into the vector pCMV6-AN-3DDK (OriGene). .. The plasmids expressing CFP-fused MAP4K2 and YFP-fused DDX39B proteins were constructed by subcloning the MAP4K2 cDNA and DDX39B cDNA from the 3xMyc- MAP4K2 plasmid and 3xFlag- DDX39B plasmid into the pCMV6-AC-CFP vector (OriGene) and pCMV6-AN-YFP vector (OriGene), respectively. ..

    Clone Assay:

    Article Title: Phosphoglycerate kinase 1 cooperates with AP-2 complex to promote endocytosis
    Article Snippet: .. For mRFP-PGK1, PGK1 was cloned and inserted into the HindIII sites of pCMV6-AN-mRFP (OriGene) with a stop codon. .. PGK1 wild-type was cloned and inserted into the BamHI and XhoI sites of the wild-type pGEX4T-3 (Amersham) vector; PGK1 wild-type were cloned and inserted into the BamHI and XhoI sites of pJG4-5.



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