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mammalian expression vector pcmv p16 ink4a  (Addgene inc)


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    Structured Review

    Addgene inc mammalian expression vector pcmv p16 ink4a
    Mammalian Expression Vector Pcmv P16 Ink4a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+vector/pCMV+p16+INK4A+(Plasmid+%2310916)/us12589163-1074-11-19
    Average 92 stars, based on 16 article reviews
    mammalian expression vector pcmv p16 ink4a - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Insights into the compact CRISPR-Cas9d system.
    Article Snippet: Cloning was performed using Golden Gate assembly with BasI-HFv2, T4 Ligase and T4PNK (New England Biolabs, R3733L, M0202L, and M0201L) following the manufacture’s protocols. .. The target plasmids were Golden Gate cloned into a modified pCMV vector (Addgene #205277) with Kanamycin resistance. ..

    Article Title: Insights into the compact CRISPR–Cas9d system
    Article Snippet: Cloning was performed using Golden Gate assembly with BasI-HFv2, T4 Ligase and T4PNK (New England Biolabs, R3733L, M0202L, and M0201L) following the manufacture’s protocols. .. The target plasmids were Golden Gate cloned into a modified pCMV vector (Addgene #205277) with Kanamycin resistance. ..

    Article Title: Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide release
    Article Snippet: .. The prototype sensor was designed in silico by sequence alignment (Clustal Omega2) and ordered as a geneblock (Thermo Fisher) flanked by HindIII and NotI restriction sites to be subsequently cloned into pCMV vector (Addgene #111053). .. For sensor optimization, site directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom designed primers using a Pfu-Ultra II fusion High Fidelity DNA Polymerase (Agilent).

    Article Title: Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release
    Article Snippet: .. The prototype sensor was designed in silico by sequence alignment (Clustal Omega2) and ordered as a geneblock (Thermo Fisher) flanked by Hind III and Not I restriction sites to be subsequently cloned into pCMV vector (Addgene #111053). .. For sensor optimization, site-directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom-designed primers (Thermo Fisher) using a Pfu-Ultra II fusion High-Fidelity DNA Polymerase (Agilent).

    Article Title: Systematic investigation of zinc finger MYND-type containing 11 mediated alternative splicing reveals notable events in prostate cancer
    Article Snippet: .. 163 For overexpression of ZMYND11, the gene was cloned into the pcDNA3.1 164 vector (Addgene, Watertown, MA, USA) for 22Rv1 and LNCaP cell lines, and 165 into the pCMV vector (Addgene, Watertown, MA, USA) for the DU145 cell line. ..

    Modification:

    Article Title: Insights into the compact CRISPR-Cas9d system.
    Article Snippet: Cloning was performed using Golden Gate assembly with BasI-HFv2, T4 Ligase and T4PNK (New England Biolabs, R3733L, M0202L, and M0201L) following the manufacture’s protocols. .. The target plasmids were Golden Gate cloned into a modified pCMV vector (Addgene #205277) with Kanamycin resistance. ..

    Article Title: Insights into the compact CRISPR–Cas9d system
    Article Snippet: Cloning was performed using Golden Gate assembly with BasI-HFv2, T4 Ligase and T4PNK (New England Biolabs, R3733L, M0202L, and M0201L) following the manufacture’s protocols. .. The target plasmids were Golden Gate cloned into a modified pCMV vector (Addgene #205277) with Kanamycin resistance. ..

    Plasmid Preparation:

    Article Title: Insights into the compact CRISPR-Cas9d system.
    Article Snippet: Cloning was performed using Golden Gate assembly with BasI-HFv2, T4 Ligase and T4PNK (New England Biolabs, R3733L, M0202L, and M0201L) following the manufacture’s protocols. .. The target plasmids were Golden Gate cloned into a modified pCMV vector (Addgene #205277) with Kanamycin resistance. ..

    Article Title: Insights into the compact CRISPR–Cas9d system
    Article Snippet: Cloning was performed using Golden Gate assembly with BasI-HFv2, T4 Ligase and T4PNK (New England Biolabs, R3733L, M0202L, and M0201L) following the manufacture’s protocols. .. The target plasmids were Golden Gate cloned into a modified pCMV vector (Addgene #205277) with Kanamycin resistance. ..

    Article Title: E2F3a activates Gadd45b gene expression through PPARα-mediated transcriptional regulation in hepatic cells
    Article Snippet: .. For mammalian expression, human E2F3a and mouse E2f8 coding regions were subcloned into the pEGFPc1 vector (RRID:Addgene_194119) or pCMV vector (RRID:Addgene_20783) using Asc I- Pac I (extra added restriction sites). ..

    Article Title: E2F3a Activates Gadd45b Gene Expression Through PPARα-Mediated Transcriptional Regulation in Hepatic Cells.
    Article Snippet: .. For mammalian expression, human E2F3a and mouse E2f8 coding regions were subcloned into the pEGFPc1 vector (RRID:Addgene_194119) or pCMV vector (RRID:Addgene_20783) using AscI-PacI (extra added restriction sites). ..

    Expressing:

    Article Title: E2F3a activates Gadd45b gene expression through PPARα-mediated transcriptional regulation in hepatic cells
    Article Snippet: .. For mammalian expression, human E2F3a and mouse E2f8 coding regions were subcloned into the pEGFPc1 vector (RRID:Addgene_194119) or pCMV vector (RRID:Addgene_20783) using Asc I- Pac I (extra added restriction sites). ..

    Article Title: E2F3a Activates Gadd45b Gene Expression Through PPARα-Mediated Transcriptional Regulation in Hepatic Cells.
    Article Snippet: .. For mammalian expression, human E2F3a and mouse E2f8 coding regions were subcloned into the pEGFPc1 vector (RRID:Addgene_194119) or pCMV vector (RRID:Addgene_20783) using AscI-PacI (extra added restriction sites). ..

    In Silico:

    Article Title: Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide release
    Article Snippet: .. The prototype sensor was designed in silico by sequence alignment (Clustal Omega2) and ordered as a geneblock (Thermo Fisher) flanked by HindIII and NotI restriction sites to be subsequently cloned into pCMV vector (Addgene #111053). .. For sensor optimization, site directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom designed primers using a Pfu-Ultra II fusion High Fidelity DNA Polymerase (Agilent).

    Article Title: Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release
    Article Snippet: .. The prototype sensor was designed in silico by sequence alignment (Clustal Omega2) and ordered as a geneblock (Thermo Fisher) flanked by Hind III and Not I restriction sites to be subsequently cloned into pCMV vector (Addgene #111053). .. For sensor optimization, site-directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom-designed primers (Thermo Fisher) using a Pfu-Ultra II fusion High-Fidelity DNA Polymerase (Agilent).

    Sequencing:

    Article Title: Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide release
    Article Snippet: .. The prototype sensor was designed in silico by sequence alignment (Clustal Omega2) and ordered as a geneblock (Thermo Fisher) flanked by HindIII and NotI restriction sites to be subsequently cloned into pCMV vector (Addgene #111053). .. For sensor optimization, site directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom designed primers using a Pfu-Ultra II fusion High Fidelity DNA Polymerase (Agilent).

    Article Title: Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release
    Article Snippet: .. The prototype sensor was designed in silico by sequence alignment (Clustal Omega2) and ordered as a geneblock (Thermo Fisher) flanked by Hind III and Not I restriction sites to be subsequently cloned into pCMV vector (Addgene #111053). .. For sensor optimization, site-directed mutagenesis and Circular Polymerase Extension Cloning was performed by polymerase chain reaction with custom-designed primers (Thermo Fisher) using a Pfu-Ultra II fusion High-Fidelity DNA Polymerase (Agilent).

    Over Expression:

    Article Title: Systematic investigation of zinc finger MYND-type containing 11 mediated alternative splicing reveals notable events in prostate cancer
    Article Snippet: .. 163 For overexpression of ZMYND11, the gene was cloned into the pcDNA3.1 164 vector (Addgene, Watertown, MA, USA) for 22Rv1 and LNCaP cell lines, and 165 into the pCMV vector (Addgene, Watertown, MA, USA) for the DU145 cell line. ..



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