pcmv mir 155 (OriGene)

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OriGene pcmv mir 155
Pcmv Mir 155, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+vector/PCMVMIR+MicroRNA+Expression+Vector/pm42240309-68-3-9
Average 95 stars, based on 242 article reviews

pcmv mir 155 - by Bioz Stars, 2026-10

95/100 stars

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Plasmid Preparation:

Article Title: Restoration of myogenesis in ALS-myocytes through miR-26a-5p-mediated Smad4 inhibition and its impact on motor neuron development.
Article Snippet: .. It permits that all cells express the miRNA avoiding the 726 influence of untransfected cells. pCMVmiR-GFP vector (Origene) containing miR-26a, -134a or -727 431 gene were used to transfect C2C12 cells. .. For the stable overexpression of miRNAs, cells were 728 cultured in the proliferative medium without antibiotics 24h prior to the transfection and 60,000 cells 729 were seeded in 24 well plates (SARSTEDT) in 900 μL DMEM GlutaMAXTM (ThermoFisher 730 Scientific), 10% FBS (ThermoFisher Scientific) without antibiotics.

Article Title: MiR-200c synergizes with trastuzumab-loaded gold nanoparticles to overcome resistance in ovarian cancer cells
Article Snippet: .. SKOV3 cells were transfected with pCMVmiR-200c (OriGene) carrying the miR200c precursor and the corresponding empty vector, pCMV (OriGene), as previously described (Anastasiadou et al. 2021). ..

Article Title: Modulating populational variance of methyl-guanine methyl transferase expression through miR-181d degradation: a novel mechanism of temozolomide resistance
Article Snippet: .. Stable cell lines constitutively expressing miR-181d were established by transfecting pCMV-miR-181d miRNA expression vector (OriGene) into CMK3 and BT-83 cells selected with neomycin as described previously ( ). ..

Article Title: MIR1255A regulates pathways critical for brain development, risk genes for depression and neurodevelopmental disorders.
Article Snippet: Vectors expressing the MIR gene under control of the Cytomegalovirus (CMV) promoter were obtained from Origene (Rockville, MD, Human MicroRNA Expression Plasmid MI0006389, SC400075). .. The control expression vector was identical to the MIR vector but contained no MIR gene (Origene CAT#: PCMVMIR). ..

Purification:

Article Title: Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer.
Article Snippet: .. Then, the purified PCR product and pCMV-miR vector Origene (Rockville, MD, USA) were digested and PCR product ligated into pCMV-miR to generate pmiR-497 using T4 DNA ligase (Thermo Scientific, USA). .. Subsequently, pmiR-497 construct was transformed into Escherichia coli TOP10 cells and then, purified by an endotoxin-free GeneJET Plasmid Maxprep Kit (Thermo Scientific, USA), according to the manufacturer’s protocol.

Polymerase Chain Reaction:

Transfection:

Stable Transfection:

Expressing:

Article Title: HTLV-1 Tax and HBZ cooperatively promote leukemogenesis through miR-155-mediated PTEN suppression and PI3K-Akt activation.
Article Snippet: Wild-type and mutant PTEN 3′-UTR reporter plasmids were generated by Sangon Biotech. .. Expression plasmids for pCMV-miR-155 and pCMV6-XL5PTEN were purchased from Origene. ..

Control:

other:

Article Title: X-Chromosome–Linked miRNAs Regulate Sex Differences in Cardiac Physiology
Article Snippet: RESULTS: Four conserved miRNAs are present in a single locus on the X-chromosome and are expressed at higher levels in females than males.. We show miRNA, miR-871, is responsible for decreased expression of the protein SRL (sarcalumenin) in females.. SRL is involved in calcium signaling, and we show it contributes to differences in electrophysiology between males and females.

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