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control empty vector pcmv mir  (OriGene)


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    Structured Review

    OriGene control empty vector pcmv mir
    Control Empty Vector Pcmv Mir, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+empty+vector+control/PCMVMIR+MicroRNA+Expression+Vector/pmc06180408-129-30-37
    Average 95 stars, based on 243 article reviews
    control empty vector pcmv mir - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: Ano6 disruption impairs acinar cell regulatory volume decrease and protein secretion in murine submandibular salivary glands.
    Article Snippet: Funding information Intramural Research Program of the National Institute of Dental and Craniofacial Research,Veterinary Research Core, Grant/Award Number: 1‐ZIC‐DE000740; Intramural Research Program of the National Institute of Dental and Craniofacial Research, Grant/Award Number: NIH 1‐ZIA‐DE000738 Abstract The widely expressed Anoctamin 6 (Ano6) supports different Ca‐dependent functions, but little is known about its role in salivary glands.. Mouse submandibular gland (SMG) acinar cells exhibited a robust regulatory volume decrease (RVD) following cell swelling that was reduced approximately 70% in Ano6 mice.. Ca‐free conditions nearly eliminated the RVD response suggesting that Ano6 is an obligatory component of the cell volume‐activated, Ca‐dependent RVD pathway in salivary gland acinar cells.

    Article Title: MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3.
    Article Snippet: The pCMV6‐myc‐DDK‐tagged human ANXA3 (oeANXA3; cat. no. RS201540) and pCMV Entry‐myc‐DDK empty vector (oeCTL; cat. no. RS100001) were purchased from OriGene Technologies, Inc and used as overexpression and negative control vectors.

    Control:

    Article Title: Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, Autophagy, and YB-1 independent manner
    Article Snippet: Briefly, 10 μg of pCMV6 encoding ANKRD22 gene (OriGene, Rockville, MD, USA) was incubated with 30 μL of TransIT-293 in 600 uL of OPT-MEM for 30 minutes at RT, and then cultured with 6×10 6 293T cells in 10 mL of D10 media in a 100 mm tissue culture dish (Becton Dickinson, Franklin Lakes, NJ, USA) for 2 days. .. As a control, empty pCMV6 vector (OriGene) was transfected into the cells. ..

    Article Title: Wnt16 Promotes Arterial Smooth Muscle Contractile Phenotype via Taz(Wwtr1) Relays, Limiting Ascending Aorta Aneurysmal Dilatation
    Article Snippet: Running title: Wnt16-Taz Signaling and Arterial Smooth Muscle †These authors contributed equivalently to the manuscript.. Address for correspondence: Dwight A. Towler MD, PhD UT Southwestern Medical Center Internal Medicine – Endocrine Division 5323 Harry Hines Blvd Dallas, TX 75390-8857 Telephone: (214) 648-2982 Email: Dwight.Towler@utsouthwestern.edu The authors have declared that no conflict of interests exist, including industry relationships.

    Plasmid Preparation:

    Article Title: Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, Autophagy, and YB-1 independent manner
    Article Snippet: Briefly, 10 μg of pCMV6 encoding ANKRD22 gene (OriGene, Rockville, MD, USA) was incubated with 30 μL of TransIT-293 in 600 uL of OPT-MEM for 30 minutes at RT, and then cultured with 6×10 6 293T cells in 10 mL of D10 media in a 100 mm tissue culture dish (Becton Dickinson, Franklin Lakes, NJ, USA) for 2 days. .. As a control, empty pCMV6 vector (OriGene) was transfected into the cells. ..

    Article Title: Wnt16 Promotes Arterial Smooth Muscle Contractile Phenotype via Taz(Wwtr1) Relays, Limiting Ascending Aorta Aneurysmal Dilatation
    Article Snippet: Running title: Wnt16-Taz Signaling and Arterial Smooth Muscle †These authors contributed equivalently to the manuscript.. Address for correspondence: Dwight A. Towler MD, PhD UT Southwestern Medical Center Internal Medicine – Endocrine Division 5323 Harry Hines Blvd Dallas, TX 75390-8857 Telephone: (214) 648-2982 Email: Dwight.Towler@utsouthwestern.edu The authors have declared that no conflict of interests exist, including industry relationships.

    Transfection:

    Article Title: Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, Autophagy, and YB-1 independent manner
    Article Snippet: Briefly, 10 μg of pCMV6 encoding ANKRD22 gene (OriGene, Rockville, MD, USA) was incubated with 30 μL of TransIT-293 in 600 uL of OPT-MEM for 30 minutes at RT, and then cultured with 6×10 6 293T cells in 10 mL of D10 media in a 100 mm tissue culture dish (Becton Dickinson, Franklin Lakes, NJ, USA) for 2 days. .. As a control, empty pCMV6 vector (OriGene) was transfected into the cells. ..

    Over Expression:

    Article Title: MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3
    Article Snippet: .. The pCMV6-myc-DDK-tagged human ANXA3 (oeANXA3; cat. no. RS201540) and pCMV Entry-myc-DDK empty vector (oeCTL; cat. no. RS100001) were purchased from OriGene Technologies, Inc and used as overexpression and negative control vectors. ..

    Negative Control:

    Article Title: MiR‑221 and miR‑222 regulate cell cycle progression and affect chemosensitivity in breast cancer by targeting ANXA3
    Article Snippet: .. The pCMV6-myc-DDK-tagged human ANXA3 (oeANXA3; cat. no. RS201540) and pCMV Entry-myc-DDK empty vector (oeCTL; cat. no. RS100001) were purchased from OriGene Technologies, Inc and used as overexpression and negative control vectors. ..

    Expressing:

    Article Title: Wnt16 Promotes Arterial Smooth Muscle Contractile Phenotype via Taz(Wwtr1) Relays, Limiting Ascending Aorta Aneurysmal Dilatation
    Article Snippet: Running title: Wnt16-Taz Signaling and Arterial Smooth Muscle †These authors contributed equivalently to the manuscript.. Address for correspondence: Dwight A. Towler MD, PhD UT Southwestern Medical Center Internal Medicine – Endocrine Division 5323 Harry Hines Blvd Dallas, TX 75390-8857 Telephone: (214) 648-2982 Email: Dwight.Towler@utsouthwestern.edu The authors have declared that no conflict of interests exist, including industry relationships.

    Luciferase:

    Article Title: Wnt16 Promotes Arterial Smooth Muscle Contractile Phenotype via Taz(Wwtr1) Relays, Limiting Ascending Aorta Aneurysmal Dilatation
    Article Snippet: Running title: Wnt16-Taz Signaling and Arterial Smooth Muscle †These authors contributed equivalently to the manuscript.. Address for correspondence: Dwight A. Towler MD, PhD UT Southwestern Medical Center Internal Medicine – Endocrine Division 5323 Harry Hines Blvd Dallas, TX 75390-8857 Telephone: (214) 648-2982 Email: Dwight.Towler@utsouthwestern.edu The authors have declared that no conflict of interests exist, including industry relationships.



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    ( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that <t>pCMV6-Rab11a</t> plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.
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    Image Search Results


    ( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that pCMV6-Rab11a plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.

    Journal: Oncotarget

    Article Title: Rab11a promotes proliferation and invasion through regulation of YAP in non-small cell lung cancer

    doi: 10.18632/oncotarget.15359

    Figure Lengend Snippet: ( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that pCMV6-Rab11a plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.

    Article Snippet: RPMI-1640 (Gibco, USA) with 10% FBS was used for cell culture. pCMV6-Rab11a plasmid and the control empty vector pCMV6 were obtained from Origene (Origene, USA).

    Techniques: Expressing, Western Blot, Quantitative RT-PCR, Plasmid Preparation, Over Expression, Wound Healing Assay, Migration