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pcmv sport6 0 empty control vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcmv sport6 0 empty control vector
    Pcmv Sport6 0 Empty Control Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+empty+vector+control/pm28601945-46-1-8
    Average 86 stars, based on 1 article reviews
    pcmv sport6 0 empty control vector - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    other:

    Article Title: Bone Marrow Stromal Cell-derived Growth Inhibitor Inhibits Growth and Migration of Breast Cancer Cells via Induction of Cell Cycle Arrest and Apoptosis
    Article Snippet: Briefly, a BMSC cDNA library was constructed in the pCMV SPORT6.0 vector (Invitrogen) using the Superscript plasmid system for cDNA synthesis and plasmid cloning (Invitrogen).

    Control:

    Article Title: CADASIL mutant NOTCH3(R90C) decreases the viability of HS683 oligodendrocytes via apoptosis.
    Article Snippet: NOTCH3(R90C) were less viable and had a higher rate of apoptosis.. Immunoblotting revealed that cells transfected with NOTCH3(R90C) had higher levels of intrinsic mitochondrial apoptosis, extrinsic death receptor path-related apoptosis, and autophagy compared with cells transfected with wild-type NOTCH3.. This study suggests that in patients with CADASIL, early defects in glia influenced by NOTCH3(R90C) may directly contribute to white matter pathology in addition to secondary vascular defects.

    Plasmid Preparation:

    Article Title: Cyclin L2, a Novel RNA Polymerase II-associated Cyclin, Is Involved in Pre-mRNA Splicing and Induces Apoptosis of Human Hepatocellular Carcinoma Cells
    Article Snippet: On the 14th day, stromal cells were activated with 10 g/ml lipopolysaccharide (Sigma) and 100 ng/ml phorbol 12-myristate 13-acetate (Sigma) for 8 h and then collected for cDNA library construction. .. A plasmid cDNA library of pCMV SPORT6.0 vector (Invitrogen) was constructed using the Superscript plasmid system (Invitrogen) for cDNA synthesis. ..

    cDNA Library Assay:

    Article Title: Cyclin L2, a Novel RNA Polymerase II-associated Cyclin, Is Involved in Pre-mRNA Splicing and Induces Apoptosis of Human Hepatocellular Carcinoma Cells
    Article Snippet: On the 14th day, stromal cells were activated with 10 g/ml lipopolysaccharide (Sigma) and 100 ng/ml phorbol 12-myristate 13-acetate (Sigma) for 8 h and then collected for cDNA library construction. .. A plasmid cDNA library of pCMV SPORT6.0 vector (Invitrogen) was constructed using the Superscript plasmid system (Invitrogen) for cDNA synthesis. ..

    Construct:

    Article Title: Cyclin L2, a Novel RNA Polymerase II-associated Cyclin, Is Involved in Pre-mRNA Splicing and Induces Apoptosis of Human Hepatocellular Carcinoma Cells
    Article Snippet: On the 14th day, stromal cells were activated with 10 g/ml lipopolysaccharide (Sigma) and 100 ng/ml phorbol 12-myristate 13-acetate (Sigma) for 8 h and then collected for cDNA library construction. .. A plasmid cDNA library of pCMV SPORT6.0 vector (Invitrogen) was constructed using the Superscript plasmid system (Invitrogen) for cDNA synthesis. ..

    cDNA Synthesis:

    Article Title: Cyclin L2, a Novel RNA Polymerase II-associated Cyclin, Is Involved in Pre-mRNA Splicing and Induces Apoptosis of Human Hepatocellular Carcinoma Cells
    Article Snippet: On the 14th day, stromal cells were activated with 10 g/ml lipopolysaccharide (Sigma) and 100 ng/ml phorbol 12-myristate 13-acetate (Sigma) for 8 h and then collected for cDNA library construction. .. A plasmid cDNA library of pCMV SPORT6.0 vector (Invitrogen) was constructed using the Superscript plasmid system (Invitrogen) for cDNA synthesis. ..



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    ( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that pCMV6-Rab11a plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.

    Journal: Oncotarget

    Article Title: Rab11a promotes proliferation and invasion through regulation of YAP in non-small cell lung cancer

    doi: 10.18632/oncotarget.15359

    Figure Lengend Snippet: ( A ) Endogenous expression of Rab11a was examined in HBE and lung cancer cell lines by western blot and RT-qPCR. Lung cancer cell lines showed significant upregulated Rab11a expression. ( B ) Western blot and RT-qPCR analysis showed that pCMV6-Rab11a plasmid markedly increases its levels in H460 and H1299 cells compared with control. Rab11a plasmid downregulated its expression in A549 cells. ( C ) MTT showed that Rab11a overexpression in H1299 and H460 cells greatly promoted the proliferation rate while Rab11a depletion inhibited proliferation rate. ( D ) Rab11a overexpression in H1299 and H460 cells promoted the colony formation ability while Rab11a depletion inhibited colony formation ability. ( E ) Rab11a overexpression in H1299 and H460 cells greatly promoted cell invasion while Rab11a depletion inhibited invading ability of A549 cells. ( F ) Wound healing assay demonstrated that Rab11a overexpression increased cell migration in H1299 and H460 cell lines while its depletion downregulated A549 cell migration.

    Article Snippet: RPMI-1640 (Gibco, USA) with 10% FBS was used for cell culture. pCMV6-Rab11a plasmid and the control empty vector pCMV6 were obtained from Origene (Origene, USA).

    Techniques: Expressing, Western Blot, Quantitative RT-PCR, Plasmid Preparation, Over Expression, Wound Healing Assay, Migration